The influence of type and timing of protein supplementation on wool growth and protein synthesis in the skin of young Merino sheep

1999 ◽  
Vol 50 (4) ◽  
pp. 497 ◽  
Author(s):  
D. G. Masters ◽  
G. Mata ◽  
S. M. Liu
Keyword(s):  
Protein Synthesis ◽  
Experimental Period ◽  
Protein Supplementation ◽  
The Other ◽  
Canola Meal ◽  
Protein Supplements ◽  
Wool Growth ◽  
Low Concentrations ◽  
High Concentrations ◽  
Dietary Treatments

There is limited evidence that the response in wool growth resulting from feeding protected protein supplements continues after the feeding has stopped. Feeding such proteins, alternated with traditional supplements, may increase wool growth as much as continuous feeding but at a lower cost. This experiment aimed to determine whether the response to protected protein continued after the sheep were switched to a cereal supplement. Over a 2-month experimental period, 56 weaners (5 months old, weighing 26 kg) were used in a 2 × 2 factorial experiment. Half were fed a diet containing 25% canola meal [partially protected protein with high concentrations of sulfur amino acids (SAA)] mixed with oaten hay, urea, and minerals. The other half were fed the same diet but with lupin seed (highly degradable protein with low concentrations of SAA) replacing the canola meal. Within each of the 2 dietary treatments and in each of 2 months, half of the weaners were fed the diet continuously, the other half were fed the diet for 2 weeks followed by 2 weeks of a barley, oats, hay, urea, and minerals diet. Another group of 8 weaners was fed the oats–barley diet continuously for 2 months. All sheep were fed to lose 35 g liveweight/day. Weaners fed canola meal grew 11% more wool during the experiment and had a higher rate of protein synthesis in the skin than weaners fed lupins. The response to canola meal of wool and skin was the same whether feeding was continuous or alternated with oats–barley, indicating that the benefits from feeding partially protected proteins continues after feeding has stopped.

scholarly journals The clinical cross-reactivity and immunological cross-antigenicity of wheat and barley

2021 ◽  
Author(s):  
Shohei Kubota ◽  
Yuji Aoki ◽  
Tomomi Sskai ◽  
Katsumasa Kitamura ◽  
Teruaki Matsui ◽  
...  
Keyword(s):  
Oral Immunotherapy ◽  
Cross Reactivity ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Allergy ◽  
Extract Concentration ◽  
Low Concentrations ◽  
High Concentrations ◽  
Food Challenges ◽  
Elisa Inhibition

Background: Some patients with a wheat allergy have been reported to show clinical cross-reactivity to barley. However, it is not clear whether the development of barley allergy in patients with a wheat allergy is due to cross-antigenicity between wheat and barley. In our study, we aimed to determine the clinical cross-reactivity and immunological cross-antigenicity of wheat and barley. Methods: We compared the results of barley oral food challenges (OFCs) before oral immunotherapy (OIT) for wheat with those after OIT in nine patients with a wheat allergy to estimate the clinical cross-reactivity of wheat and barley. Moreover, we performed enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblotting inhibition using serum from seven patients allergic to wheat and barley. Results: Nine patients who had positive barley-OFC results performed before OIT for wheat were all negative on barley-OFC performed after OIT. In ELISA inhibition, preincubation of serum from patients allergic to wheat and barley with a high barley extract concentration inhibited binding of IgE to wheat extract by less than 10%. On the other hand, wheat and barley extracts equally inhibited binding to barley sIgE at high concentrations. In the immunoblotting inhibition test, the spots of wheat were inhibited but weakly by barley extracts, and most of the spots of barley were inhibited even by low concentrations of the wheat and barley extract. Conclusion: We showed that barley allergy associated with wheat allergy is caused by cross-reactivity from wheat. The OIT for wheat was one of the promising options for barley allergy.

Impacts of rapeseed meal, canola meal and their mixture substitute for soybean meal on performance of lactating sows and their offspring

2018 ◽  
Author(s):  
Wen Chao Liu ◽  
Shi Hui Zhou ◽  
Yong Min Kim ◽  
Sang In Lee ◽  
Huan Ying Pang and In Ho Kim
Keyword(s):  
Soybean Meal ◽  
Body Weight Loss ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
Canola Meal ◽  
Negative Effects ◽  
Lactating Sows ◽  
Dietary Treatments ◽  
The Impact ◽  
Piglet Survival

The present study was to evaluate the impact of rapeseed meal (RSM), canola meal (CM), and their mixture substitute for soybean meal on performance of lactating sows and their offspring. In total, 16 Yorkshire×Landrace sows with initial BW 258.87±5.36 kg were used in this trial. The experimental period lasted 30 days. The sows were allotted to one of four dietary treatments (4 sows per treatment): 1) corn-soybean meal (SBM) basal diets; 2) basal diets containing 8% RSM; 3) basal diets containing 8% CM; 4) basal diets containing RSM (4%) and CM (4%) mixture (RSCM). Sows fed with RSM and RSCM diets had a higher (P less than 0.05) body weight loss than sows fed SBM diet. The parity, litter number, piglet survival rate, weaning pig number, backfat, ADFI and estrus interval were not influenced by dietary treatments (P greater than 0.05). The ADG of piglets from sows fed SBM was higher (P less than 0.05) than sows fed RSM at 1 week. After farrowing, the apparent total tract digestibility (ATTD) of dry matter in sows fed with SBM diet was higher (P less than 0.05) than the RSCM group. The ATTD of energy in SBM and CM treatments was higher (P less than 0.05) than that in RSM and RSCM treatments. On the weaning day, the ATTD of nitrogen and energy in SBM treatment was highest (P less than 0.05) than other treatments. In conclusion, inclusion of 8% rapeseed meal, canola meal, and their mixture substitute for soybean meal had negative effects on reproductive performance of lactating sows and their offspring.

Fibrinolytic Activity of Thrombin Preparations

1964 ◽  
Vol 11 (01) ◽  
pp. 234-242 ◽  
Author(s):  
Pieter Brakman ◽  
Panpit Klug ◽  
Tage Astrup
Keyword(s):  
Protease Activity ◽  
Fibrinolytic Activity ◽  
The Other ◽  
Fibrinolytic Agents ◽  
Fibrinolytic Agent ◽  
Low Concentrations ◽  
Fibrin Plate ◽  
Simple Chemical ◽  
Per Se ◽  
High Concentrations

SummarySome thrombin samples have a slight unspecific protease activity probably caused by contaminating plasmin. All investigated samples were fibrinolytically active. This activity was caused by an activator of plasminogen. Fibrinolytic activity was apparently produced by two components of the thrombin preparations. One of these components was a contaminant with fibrinolytic activity but with no thrombin activity. This component could be separated from the thrombin by simple chemical procedures. The other fibrinolytic component appeared to be the thrombin molecule per se. It was not fibrinolytically active when used in the low concentrations required for clotting of fibrinogen, but in high concentrations, assayed on the fibrin plate, it activated plasminogen. In the accurate assay of fibrinolytic agents it is necessary to use preparations of thrombin from which the contaminating fibrinolytic agent has been removed.

Transport and exchange diffusion of l-tryptophan in Ehrlich cells

1961 ◽  
Vol 200 (5) ◽  
pp. 1063-1068 ◽  
Author(s):  
John A. Jacquez
Keyword(s):  
Amino Acid ◽  
Initial Velocity ◽  
The Other ◽  
Preliminary Loading ◽  
Exchange Diffusion ◽  
Diaminobutyric Acid ◽  
Low Concentrations ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
High Concentrations

The initial velocity of uptake of l-tryptophan by Ehrlich ascites cells can be explained as the sum of two processes: diffusion and an active transport that shows a saturation effect. Azaserine, l-2,4 diaminobutyric acid, l-histidine, and l-leucine, at low concentrations, increase the initial velocity of uptake of l-tryptophan but compete with l-tryptophan at high concentrations. Preliminary loading of the cells with glycine decreases the initial tryptophan flux: preliminary loading of the ascites cells with azaserine or tryptophan markedly increases the initial flux of uptake of the other amino acid.

PATTERNS OF PLASMA PROLACTIN IN POST-PARTUM SUCKLED COWS

1981 ◽  
Vol 90 (3) ◽  
pp. 391-396 ◽  
Author(s):  
R. WEBB ◽  
G. E. LAMMING
Keyword(s):  
Post Partum ◽  
Significant Negative Correlation ◽  
Prolactin Secretion ◽  
The Other ◽  
Plasma Prolactin ◽  
Blood Samples ◽  
Biphasic Pattern ◽  
Stage Of Lactation ◽  
Low Concentrations ◽  
High Concentrations

Blood samples taken on alternate days through indwelling jugular venous catheters from 12 suckled cows between days 14 and 48 post partum contained significantly less prolactin than samples collected on intermediate days by jugular venepuncture. Samples taken through the catheter every 2 h for 72 h periods revealed a repetitive daily biphasic pattern of prolactin secretion with low concentrations at 09.00 and 19.00 h and high concentrations at 13.00 and 23.00 h. In two groups of cows, one group calving at the beginning of March (increasing photoperiod) and the other calving during June (decreasing photoperiod), there was a significant negative correlation between stage of lactation and plasma prolactin concentrations in samples taken by venepuncture.

Influence of liveweight, liveweight change, and diet on wool growth, staple strength, and fibre diameter in young sheep

1998 ◽  
Vol 49 (2) ◽  
pp. 269 ◽  
Author(s):  
D. G. Masters ◽  
A. D. Peterson ◽  
G. Mata ◽  
S. M. Liu
Keyword(s):  
Management Strategy ◽  
Fibre Diameter ◽  
Experimental Period ◽  
Canola Meal ◽  
Wool Growth ◽  
Lupin Seed ◽  
Staple Strength ◽  
Wool Yield ◽  
Fleece Weight

The effects of initial liveweight, liveweight change, and composition of the diet on wool growth and staple strength in weaner sheep were investigated. Weaner wethers (64) were allocated to treatments in a factorial design (23). The factors were initial liveweight (heavy, 33 kg; light, 25 kg), liveweight change (fed at maintenance for 56 days, or 28 days at 0 ·6×maintenance then 28 days at 1·6×maintenance), and diet (lupin seed based diet or canola meal based diet). The heavier weaners grew approximately 1·9 g/day more wool than the light sheep during the experimental period and this resulted in a higher clean fleece weight (1·8 v. 1·4 kg), staple strength (37 v. 27·4 N/ktex), and fibre diameter (18·0 v. 17·4 µm). Substituting canola meal for lupin seed increased wool growth during the experiment and clean wool yield, but did not affect clean fleece weight or staple strength. The weaners fed to lose and then gain weight grew wool with a lower staple strength than the weaners fed to maintain weight through the experiment (28·6 v. 35·8 N/ktex), but there were no differences in any of the other fleece characteristics. The ratio of wool growth to dry matter intake (DMI) was higher in the sheep fed canola meal than those fed lupins (by approximately 1·5 g/kg DMI) and higher in weaners losing weight than those fed to maintain weight (by approximately 4·5 g/kg DMI). From a practical perspective, these results indicate that initial liveweight and liveweight change both influence staple strength and wool growth and need to be included in any management strategy to improve wool quality of young sheep.

Existence of two types of postjunctional α-adrenoceptors in the isolated canine internal carotid artery

1988 ◽  
Vol 66 (5) ◽  
pp. 655-659 ◽  
Author(s):  
Yasuaki Kawai ◽  
Shigeaki Kobayashi ◽  
Toshio Ohhashi
Keyword(s):  
Response Curve ◽  
Carotid Arteries ◽  
Internal Carotid ◽  
The Other ◽  
Low Concentrations ◽  
Selective Agonists ◽  
High Concentrations ◽  
The Right ◽  
Internal Carotid Arteries ◽  
Concentration Response Curve

The pharmacological characteristics of postjunctional α-adrenoceptors in isolated canine internal carotid arteries were investigated by the use of selective agonists and antagonists for α1 and α2-adrenoceptors. Norepinephrine, phenylephrine, and xylazine caused concentration-dependent contractions in the helical strips. The contraction induced by 10−4 M xylazine was significantly smaller than that produced by 10−4 M norepinephrine or 10−4 M phenylephrine. The contraction induced by 10−4 M phenylephrine was almost the same value as that induced by 10−4 M norepinephrine. Phentolamine (10−8 and 10−7 M) caused a parallel shift to the right of the concentration–response curve to norepinephrine. The contractile responses to low concentrations of norepinephrine were significantly suppressed by pretreatment with an α2-antagonist such as yohimbine (10−9 and 10−8 M) or DG 5128(10−7 and 10−6 M). On the other hand, the responses to higher concentrations of norepinephrine were mainly reduced by low concentrations of an α1-antagonist, prazosin (3 × 10−10 and 3 × 10−9 M). These results suggest that both α1- and α2-adrenoceptors are located on the plasma membrane of smooth muscle cells in canine internal carotid arteries and that the norepinephrine-induced contractions at low and high concentrations are mainly mediated by activation of α2- and α1-adrenoceptors, respectively.

Chromium and protein supplements for growing-finishing beef steers fed barley-based diets

1995 ◽  
Vol 75 (4) ◽  
pp. 549-558 ◽  
Author(s):  
G. W. Mathison ◽  
D. F. Engstrom
Keyword(s):  
Stress Protein ◽  
Protein Supplementation ◽  
Canola Meal ◽  
Measured Parameter ◽  
Detectable Effect ◽  
Feed Conversion ◽  
Or Efficiency ◽  
Protein Supplements ◽  
Growing Period ◽  
Barley Silage

The effect of chromium supplementation on rate and efficiency of gain and morbidity in normally managed calves and calves with additional imposed stress was assessed in a 28-d trial involving 192 crossbred steer calves (262 ± 20 kg) in exp. 1. Imposed stress consisted of withholding of feed and water for an additional 12 h after arrival in the feedlot and mixing cattle 12 and 72 h after arrival. Chromium supplementation had no influence on rate of gain or efficiency of gain or on morbidity during the initial 28 d in the feedlot. Additional imposed stress increased weight loss from 7.0 kg to 14 kg during first 10 d in the feedlot and was associated with a 19% reduction (P < 0.001) in feed dry matter (DM) intake during the first 10 d. However, by 28 d no differences in daily gain or feed conversion due to imposed stress were detected. Morbidities during exp. 1 were 31% for normally managed calves and 44% for stressed steers (P = 0.05). In exp. 2, the effect of chromium supplementation, maturity of barley silage, and type of protein supplement [control, urea, canola meal, and blood meal/corn gluten meal (bypass protein)], on rate and efficiency of gain and carcass characteristics was determined in a growing-finishing experiment using the calves from exp. 1. Supplemental chromium had no detectable effect on any measured parameter when a barley silage-based diet (silage 68% of DM) was fed for 70 d or when a high concentrate diet (concentrate 86% of DM) diet was fed for 91 d. Maturity of silage did not affect rate or efficiency of gain in the growing period however fewer steers (P = 0.03) fed the more mature silage were sick than when silage harvested 10 d earlier was fed (morbidity of 3 vs. 12%). During the 70-d growing period steers supplemented with canola meal tended (P = 0.06) to consume more DM than those fed the control or bypass protein supplements (7.84 vs. 7.37 and 7.38 kg d−1). However, no differences in rate or efficiency of gain were detected in this period. During the 91 d finishing period steers fed canola meal supplement consumed more DM (P = 0.004) than those fed control or bypass supplements. The amounts of DM consumed per kg gain during this period were 6.07, 6.05, 6.09, and 5.79 kg (P = 0.09) for steers fed the control, urea, canola, and bypass supplements, respectively. It was concluded that rate and efficiency of gain and incidence of sickness were not influenced by chromium supplementation either in the first 28 d in the feedlot or throughout the total feeding period. Protein supplementation had no detectable influence on rate or efficiency of gain in either the growing or finishing penods. Key words: Chromium, stress, protein, calves, gain, feed efficiency

The potential of isobutyraldehyde monourea (propanal, 2-methyl-monourea) as a nonprotein nitrogen source for ruminant animals

1994 ◽  
Vol 74 (4) ◽  
pp. 665-674
Author(s):  
G. W. Mathison ◽  
R. Soofi-Siawash ◽  
M. Worsley
Keyword(s):  
Soybean Meal ◽  
Slow Release ◽  
Isobutyric Acid ◽  
The Other ◽  
Canola Meal ◽  
Nonprotein Nitrogen ◽  
Nitrogen Supplementation ◽  
Protein Supplements ◽  
Ruminant Animals

Experiments were earned out with sheep and in vitro to evaluate isobutyraldehyde monourea (IBMU; propanal-2-methyl-monourea) as a slow-release nitrogen source for ruminant animals. Examination of the compound for carbon, hydrogen, and nitrogen composition and infrared, NMR and mass spectral analyses indicated that IBMU was present in significant amounts. Ten sheep were randomly assigned to diets containing bromegrass hay and corn grain and the following nitrogen supplementation treatments: (1) control, (2) urea, (3) soybean meal, (4) canola meal and (5) IBMU, with the protein supplements providing approximately 25% of the nitrogen intake. Similar treatments were also prepared and evaluated in vitro, with the exception that wheat straw rather than bromegrass hay was used in the incubation. The digestibility of nitrogen in the ration containing IBMU was higher (P < 0.05) than its digestibility in the other rations when measurements were conducted with sheep over three 14-d periods. No differences in dry matter (DM) or energy digestibility were detected between the different treatments. The digestible energy content of IBMU was estimated to be greater than 21 MJ kg−1. The digestibilities of DM in vitro after 2, 4, 8, 12, 24, 48 and 72 h of incubation were not influenced by the presence of IBMU. No influence of nitrogen supplementation on nitrogen balance was observed, probably because of the high protein content of the control ration (10.6%). IBMU exhibited the characteristics of an ideal slow-release nonprotein nitrogen supplement, since concentrations of rumen ammonia nitrogen did not vary from 0 to 8 h after feeding and ammonia concentrations were higher (P < 0.05) both before feeding and 8 h after feeding when IBMU was fed than when any of the other supplements were fed. The concentration of isobutyric acid averaged 2.6-fold higher when IBMU was fed than when canola meal or soybean meal was fed, confirming that most of the compound was degraded in the rumen. Sheep fed IBMU had lower concentrations of acetic, propionic and butyric acid in the rumen than those fed natural protein supplements. It was unclear whether the decrease in VFA concentration was positive or negative in terms of nutritional value. It was concluded that IBMU exhibited some ideal characteristics for a nonprotein nitrogen supplement for ruminant animals because it was degraded at an optimum rate in the rumen and provided rumen microflora with isobutyric acid. Further experimentation is warranted to determine its usefulness when nitrogen-deficient diets are fed to ruminant animals. Key words: Nonprotein nitrogen, isobutyric acid, isobutyraldehyde, slow-release, isobutyraldehyde monourea, propanal-2-methyl-monourea

ION PAIRS IN A SOLONETZIC SOIL

1983 ◽  
Vol 63 (3) ◽  
pp. 479-484 ◽  
Author(s):  
A. ALZUBAIDI ◽  
G. R. WEBSTER
Keyword(s):  
Ion Pairs ◽  
Soil Samples ◽  
The Other ◽  
Sulfate Ion ◽  
Carbonate Ion ◽  
Low Concentrations ◽  
Solonetzic Soil ◽  
Ap Horizon ◽  
Ion Activities ◽  
High Concentrations

The kinds and concentrations of the major ion pairs were determined in saturation extracts of 141 soil samples collected from a Solonetzic soil treated with various kinds of tillage combined with surface-applied chemical amendments. The correlations between concentrations of ion pairs and EC and pH of saturation extracts were statistically tested. Sulfate ion pairs NaSO4−, MgSO40 and CaSO40 occurred in relatively high concentrations. The other ion pairs were of low concentrations. Of the total soluble Ca, ion pairs ranged from 20.8% in the Ap horizon to 50.5% in the Csk horizon and Mg was approximately the same. The comparable values for Na were 0.8% and 4.6%, respectively. Ion pairs NaSO4−, KSO4−, MgSO40 and CaSO40 were significantly correlated with EC. Only carbonate ion pairs were significantly correlated with pH. Correcting concentrations of Na, Ca and Mg for ion pairs and activities changed considerably the SAR values in the Bnt1 horizon. Key words: Ion pairs, Solonetzic soils, ion activities

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