Chromium and protein supplements for growing-finishing beef steers fed barley-based diets

1995 ◽  
Vol 75 (4) ◽  
pp. 549-558 ◽  
Author(s):  
G. W. Mathison ◽  
D. F. Engstrom
Keyword(s):  
Stress Protein ◽  
Protein Supplementation ◽  
Canola Meal ◽  
Measured Parameter ◽  
Detectable Effect ◽  
Feed Conversion ◽  
Or Efficiency ◽  
Protein Supplements ◽  
Growing Period ◽  
Barley Silage

The effect of chromium supplementation on rate and efficiency of gain and morbidity in normally managed calves and calves with additional imposed stress was assessed in a 28-d trial involving 192 crossbred steer calves (262 ± 20 kg) in exp. 1. Imposed stress consisted of withholding of feed and water for an additional 12 h after arrival in the feedlot and mixing cattle 12 and 72 h after arrival. Chromium supplementation had no influence on rate of gain or efficiency of gain or on morbidity during the initial 28 d in the feedlot. Additional imposed stress increased weight loss from 7.0 kg to 14 kg during first 10 d in the feedlot and was associated with a 19% reduction (P < 0.001) in feed dry matter (DM) intake during the first 10 d. However, by 28 d no differences in daily gain or feed conversion due to imposed stress were detected. Morbidities during exp. 1 were 31% for normally managed calves and 44% for stressed steers (P = 0.05). In exp. 2, the effect of chromium supplementation, maturity of barley silage, and type of protein supplement [control, urea, canola meal, and blood meal/corn gluten meal (bypass protein)], on rate and efficiency of gain and carcass characteristics was determined in a growing-finishing experiment using the calves from exp. 1. Supplemental chromium had no detectable effect on any measured parameter when a barley silage-based diet (silage 68% of DM) was fed for 70 d or when a high concentrate diet (concentrate 86% of DM) diet was fed for 91 d. Maturity of silage did not affect rate or efficiency of gain in the growing period however fewer steers (P = 0.03) fed the more mature silage were sick than when silage harvested 10 d earlier was fed (morbidity of 3 vs. 12%). During the 70-d growing period steers supplemented with canola meal tended (P = 0.06) to consume more DM than those fed the control or bypass protein supplements (7.84 vs. 7.37 and 7.38 kg d−1). However, no differences in rate or efficiency of gain were detected in this period. During the 91 d finishing period steers fed canola meal supplement consumed more DM (P = 0.004) than those fed control or bypass supplements. The amounts of DM consumed per kg gain during this period were 6.07, 6.05, 6.09, and 5.79 kg (P = 0.09) for steers fed the control, urea, canola, and bypass supplements, respectively. It was concluded that rate and efficiency of gain and incidence of sickness were not influenced by chromium supplementation either in the first 28 d in the feedlot or throughout the total feeding period. Protein supplementation had no detectable influence on rate or efficiency of gain in either the growing or finishing penods. Key words: Chromium, stress, protein, calves, gain, feed efficiency

The influence of type and timing of protein supplementation on wool growth and protein synthesis in the skin of young Merino sheep

1999 ◽  
Vol 50 (4) ◽  
pp. 497 ◽  
Author(s):  
D. G. Masters ◽  
G. Mata ◽  
S. M. Liu
Keyword(s):  
Protein Synthesis ◽  
Experimental Period ◽  
Protein Supplementation ◽  
The Other ◽  
Canola Meal ◽  
Protein Supplements ◽  
Wool Growth ◽  
Low Concentrations ◽  
High Concentrations ◽  
Dietary Treatments

There is limited evidence that the response in wool growth resulting from feeding protected protein supplements continues after the feeding has stopped. Feeding such proteins, alternated with traditional supplements, may increase wool growth as much as continuous feeding but at a lower cost. This experiment aimed to determine whether the response to protected protein continued after the sheep were switched to a cereal supplement. Over a 2-month experimental period, 56 weaners (5 months old, weighing 26 kg) were used in a 2 × 2 factorial experiment. Half were fed a diet containing 25% canola meal [partially protected protein with high concentrations of sulfur amino acids (SAA)] mixed with oaten hay, urea, and minerals. The other half were fed the same diet but with lupin seed (highly degradable protein with low concentrations of SAA) replacing the canola meal. Within each of the 2 dietary treatments and in each of 2 months, half of the weaners were fed the diet continuously, the other half were fed the diet for 2 weeks followed by 2 weeks of a barley, oats, hay, urea, and minerals diet. Another group of 8 weaners was fed the oats–barley diet continuously for 2 months. All sheep were fed to lose 35 g liveweight/day. Weaners fed canola meal grew 11% more wool during the experiment and had a higher rate of protein synthesis in the skin than weaners fed lupins. The response to canola meal of wool and skin was the same whether feeding was continuous or alternated with oats–barley, indicating that the benefits from feeding partially protected proteins continues after feeding has stopped.

Comparison of lignosulfonate-treated canola meal and soybean meal as rumen undegradable protein supplements for lambs

1995 ◽  
Vol 75 (3) ◽  
pp. 371-377 ◽  
Author(s):  
K. Stanford ◽  
T. A. McAllister ◽  
Z. Xu ◽  
K.-J. Cheng ◽  
M. Pickard
Keyword(s):  
Soybean Meal ◽  
Average Daily Gain ◽  
Canola Meal ◽  
Feed Conversion ◽  
Protein Supplements ◽  
Rumen Undegradable Protein ◽  
Daily Gain ◽  
Growth Experiments

An experiment was undertaken to determine the effect of lignosulfonate (LSO3) treatment on the rumen-undegradable protein (RUDP) value of canola meal (CM) and soybean meal (SM). Canola meal and SM were treated with 7% LSO3 and heated (95 °C) for 1 h. Treated and untreated meals were compared in vitro, in situ and in digestibility and growth experiments using Romanov × Suffolk and Romanov × Dorset lambs. Lambs were fed four barley-based diets [13.5% crude protein (CP)] with one of CM, SM, LSO3-treated CM (LSO3-CM) or LSO3-treated SM (LSO3-SM) as protein supplements. Lignosulfonate treatment increased acid detergent insoluble nitrogen (ADIN) five-fold in SM and threefold in CM. After 6 h incubation with ruminal fluid, ammonia production from LSO3-treated meals was one-half that from untreated meals. Lignosulfonate treatment reduced (P < 0.01) in situ rates of dry matter (DM) and protein disappearance and the soluble DM and protein fractions for both SM and CM, but not the potentially digestible fractions. No differences in feed conversion or average daily gain (P > 0.05) were found among the treatments. Although LSO3 treatment did not improve the ADG of lambs fed barley-based diets, it increased the RUDP value of SM and CM without adverse effects on digestibility. Key words: Lignosulfonate, canola meal, soybean meal, lamb

scholarly journals Utilization of canola and sunflower meals as replacements for soybean meal in a corn silage-based stocker system1

2017 ◽  
Vol 1 (4) ◽  
pp. 592-598 ◽  
Author(s):  
J. M. Lourenço ◽  
M. A. Froetschel ◽  
J. R. Segers ◽  
J. J. Tucker ◽  
R. L. Stewart
Keyword(s):  
Soybean Meal ◽  
In Vitro Digestion ◽  
Corn Silage ◽  
Canola Meal ◽  
Feed Conversion ◽  
Protein Supplements ◽  
Daily Feeding ◽  
Feed Costs ◽  
And Performance

Abstract Two experiments were conducted to evaluate 3 silage-based stocker diets. In Exp. 1, diets were fed to a total of 276 animals over a period of 3 yr and performance data was collected. In Exp. 2, the same diets were subjected to in vitro digestion for 5 time periods: 0, 6, 12, 24, and 48 h, to evaluate IVDMD, production of fermentation end products, and efficiency of transformation of energy. The experimental diets were similar, except for their protein supplements. They were composed of: 1) 74% corn silage, 15.2% ground ear corn, and 10.8% soybean meal (SBM); 2) 74.4% corn silage, 9.8% ground ear corn, and 15.8% canola meal (CAN); 3) 74.5% corn silage, 9.8% ground ear corn, and 15.7% sunflower meal (SUN). Results from Exp. 1 showed that DMI was similar across all treatments (P = 0.167), but ADG was greater (P = 0.007) for animals fed either SBM or CAN than for animals fed SUN (1.29, 1.28, and 1.20 kg/d, respectively). Both CAN and SUN significantly reduced (P &lt; 0.001) daily feeding cost per animal in comparison to SBM. Exp. 2 revealed that total VFA production was similar for all treatments (P = 0.185), and greatest molar proportions of propionate were observed for SBM and CAN (P = 0.02). Additionally, IVDMD was highest for SBM (P &lt; 0.001). Regression analysis showed that most of the evaluated traits followed a quadratic trend for incubation times (P ≤ 0.02). On average, the in vitro technique used in this study was able to account for 97.03% of the caloric transformations suffered by DE throughout the different incubation times. Overall, our findings revealed that although animals receiving SUN had the cheapest daily feeding cost, important traits like ADG and feed conversion rate were negatively affected by this treatment. In contrast, data showed that CAN was an effective replacement for SBM for it maintained similar animal performance while decreasing feed costs. Therefore, from a producer standpoint, CAN is a viable alternative to replace the more costly SBM diet in silage-based stocker operations.

Protein supplementation of cows grazing limited amounts of paspalum (Paspalum dilatatum Poir.)-dominant irrigated pasture in mid-lactation

2000 ◽  
Vol 40 (7) ◽  
pp. 923 ◽  
Author(s):  
W. J. Wales ◽  
D. W. Dellow ◽  
P. T. Doyle
Keyword(s):  
Milk Production ◽  
Cottonseed Meal ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Protein Supplementation ◽  
Canola Meal ◽  
Paspalum Dilatatum ◽  
Protein Supplements ◽  
Cereal Grain

Two experiments were undertaken in summer to investigate production responses by dairy cows offered paspalum (Paspalum dilatatum Poir.)-dominant irrigated pasture to the inclusion of protein supplements with different rumen degradable protein characteristics in cereal grain-based concentrates. In experiment 1, cows in mid lactation were offered limited amounts of herbage, at an allowance of 28 kg DM/cow. day together with 8.0 kg DM/cow. day of a pelleted supplement containing: (i) mainly barley and wheat; (ii) barley, wheat and canola meal; or (iii) barley, wheat and cottonseed meal for 35 days. The 3 supplements were formulated to have similar in vitro DM digestibilities. In experiment 2 aspects of rumen function were measured in 9 rumen fistulated lactating cows, individually fed indoors on paspalum-dominant herbage harvested daily with a finger bar mower. Cows were offered the same amount of supplements for 28 days. Pasture intakes were similar to those of the grazing cows in experiment 1. In experiment 1, there were no effects (P>0.05) of dietary treatment on herbage intake (10.2 kg DM/cow. day) or selection of nutrients from herbage. The concentration of crude protein in the total diet consumed was lower (P<0.05) for the barley + wheat treatment (137 g/kg DM), compared with the barley + wheat + canola meal and barley + wheat + cottonseed meal treatments, which were 179 and 183 g/kg DM, respectively. In vitro DM digestibility of the feed consumed by cows was similar across the treatments, and all diets contained greater than 400 g neutral detergent fibre/kg DM, indicating there were no limitations due to dietary fibre. There were no significant effects (P>0.05) of dietary treatments on milk production (21.8 kg/cow. day), milk fat concentration (34.1 g/kg) or milk protein concentration (29.8 g/kg). Urea-N was higher (P<0.01) in the milk of cows fed the barley + wheat + canola meal and the barley + wheat + cottonseed meal treatments compared with the barley + wheat treatment. In experiment 2, rumen fluid pH was higher in cows consuming barley + wheat pellets than in cows consuming barley + wheat + canola meal on most sampling occasions. Rumen ammonia-N concentration was lowest in the barley + wheat treatment and highest in the barley + wheat + canola meal treatment. When cows grazing paspalum-dominant irrigated pasture were supplemented with 8 kg DM of a cereal-based supplement, metabolisable protein was calculated to be present in sufficient quantities to support at least 22 kg milk/cow. day and, hence, was not limiting milk production. It is concluded that protein supplements offered to cows in mid-lactation, grazing irrigated pastures in summer, are unlikely to give a milk production response beyond that achieved with cereal grain.

Performance of growing steers fed either hay or silage supplemented with canola or fish meal

1993 ◽  
Vol 73 (1) ◽  
pp. 57-65 ◽  
Author(s):  
J. R. Seoane ◽  
A-M. Christen ◽  
A. Amyot ◽  
H. V. Petit
Keyword(s):  
Feed Efficiency ◽  
Crude Protein ◽  
Fish Meal ◽  
Early Spring ◽  
Protein Supplementation ◽  
Canola Meal ◽  
Protein Supplements ◽  
Medium Quality ◽  
Grazing Period ◽  
Supplemental Protein

One hundred and eighty-six crossbred steers (194.4 ± 1.5 kg) were used to determine the effect of protein supplementation of medium-quality grass hay or silage on performance during wintering and compensatory growth during the grazing period. All steers received the hay or the silage ad libitum during the 182-d wintering period. They were also fed fixed quantities of one of three supplements: C, a control with no supplemental protein; CM, a canola meal; and FM, a fish meal containing supplements. Daily amounts of supplementation were fixed to supply to each steer the same amount of barley (85 g 100 kg−1 BW), of dry molasses (15 g 100 kg−1 BW) and of minerals, vitamins and Bovatec (10 g 100 kg−1 BW). The protein supplements CM and FM supplied the equivalent of 95 g of crude protein daily 100 kg−1 BW compared with 15 g 100 kg−1 BW for the control. Expressed as g kg−0.75, forage DM intake was 13.3% higher when fed as hay than as silage (P < 0.0001). Protein supplementation increased forage DM intake when fed as hay (P < 0.02) but not when fed as silage. Average daily gains (ADG) tended to be 5.8% higher for steers fed hay than for those fed silage (P = 0.06). Protein supplementation markedly improved ADG (P < 0.0001). When hay was fed, no differences were found in ADG between CM and FM supplementation; however, FM supplementation produced higher ADG than CM when silage was fed (P < 0.001). Feed efficiency was not affected by the type of forage fed, but it was higher for steers receiving protein supplementation (P < 0.001), the effect being more evident when the forage was fed as silage (P < 0.001) than when fed as hay (P = 0.07). Compensatory gains were obtained when the steers were taken out to pasture in early spring; steers fed silage, which obtained smaller ADG during wintering, showed higher ADG in pasture (P < 0.01). After 62 d grazing, the effect of protein supplementation during winter on body weight had almost disappeared. Key words: Protein supplementation, forages, cattle, growth

Effects of concentrate proportion and protein supplementation on performance of growing and finishing crossbred bulls fed a whole-crop barley silage-based diet

2014 ◽  
Vol 54 (9) ◽  
pp. 1399 ◽  
Author(s):  
M. Pesonen ◽  
E. Joki-Tokola ◽  
A. Huuskonen
Keyword(s):  
Energy Intake ◽  
Crude Protein ◽  
Dry Matter ◽  
Rapeseed Meal ◽  
Protein Supplementation ◽  
Protein Supplements ◽  
Carcass Conformation ◽  
Fat Score ◽  
Dietary Treatments ◽  
Barley Silage

A 2 × 3 factorial design was used to study the effects of concentrate proportion and protein supplementation on performance of growing and finishing bulls fed a whole-crop barley silage (WCBS)-based diet. A feeding experiment comprised 30 crossbred bulls with average initial liveweight of 315 kg. The bulls were offered WCBS ad libitum. The two concentrate proportions were 200 and 400 g/kg diet dry matter (DM). The concentrate used was (1) rolled barley, (2) rolled barley plus rapeseed meal or (3) rolled barley plus urea-based supplement. The crude protein (CP) content of rolled barley was 119 g/kg DM and both protein supplements were given so that the CP content of the concentrate was raised to 170 g/kg DM. The final liveweight of the bulls was 669 kg, on average, and it was not affected by dietary treatments. The dietary treatments had no effects on total DM intake of the bulls (8.91 kg/day). However, the increasing concentrate proportion increased energy intake (P < 0.05), carcass gain (P < 0.01) and dressing proportion (P < 0.05) and improved carcass conformation (P < 0.05). Protein supplementation had no effects on energy intake, carcass gain, dressing proportion or carcass conformation but it tended to increase carcass fat score (P = 0.06) compared to the bulls fed without protein supplementation. Overall, the results indicate that there is no reason to use protein supplementation for growing and finishing bulls more than 300-kg liveweight when they are fed with good quality whole-crop barley silage and barley-based concentrate.

Comparison of sweet white lupin seed, canola meal and soybean meal as protein supplements for lambs

1996 ◽  
Vol 76 (2) ◽  
pp. 215-219 ◽  
Author(s):  
K. Stanford ◽  
B. M. Lees ◽  
T. A. McAllister ◽  
Z. J. Xu ◽  
K.-J. Cheng
Keyword(s):  
Soybean Meal ◽  
Average Daily Gain ◽  
White Lupin ◽  
Canola Meal ◽  
Protein Supplement ◽  
Feed Conversion ◽  
Protein Supplements ◽  
Lupin Seed ◽  
Matter Energy

Western-Canadian grown sweet white lupin seed (LS), canola meal (CM) and soybean meal (SM) were compared in situ and in digestibility and feedlot experiments using Romanov × Suffolk lambs. Barley-based (75.5% as fed) diets were isonitrogenous (15.5% crude protein, CP) with LS, CM or SM as protein source. Sunflower hulls were substituted as necessary for protein supplement to ensure the diets were isonitrogenous. The LS used in this trial was relatively low in alkaloids (0.03%), as compared with other lupins, and high in manganese (2347 mg kg−1). In situ, the effective rumen degradability of protein of LS was higher than that of either CM or SM. Rates of DM and protein disappearance were also higher for LS than for CM and SM. Digestibilities of DM organic matter, energy and nitrogen were higher (P < 0.05) in LS than in CM or SM diets, but there were no differences (P > 0.05) among the supplements in digestibilities of starch or NDF. No differences (P > 0.05) were found in feed conversion or DM intake of the three diets. Average daily gain (ADG) of the lambs receiving LS did not differ (P > 0.05) from those of lambs on the other two diets, but lambs on CM had higher ADG (P < 0.05) than lambs on SM. We conclude that LS is comparable to SM and CM as a protein supplement for lambs, provided care is taken to avoid toxic levels of manganese and alkaloids. Key words: Lupin, lamb, protein, canola meal, soybean meal

LYSINE SUPPLEMENTATION OF GROWER AND FINISHER PIG DIETS BASED ON HIGH PROTEIN BARLEY, WHEAT AND SOYBEAN MEAL OR CANOLA MEAL, WITH OBSERVATIONS ON THYROID AND ZINC STATUS

1988 ◽  
Vol 68 (3) ◽  
pp. 931-940 ◽  
Author(s):  
J. M. BELL ◽  
M. O. KEITH ◽  
C. S. DARROCH
Keyword(s):  
Soybean Meal ◽  
Average Daily Gain ◽  
Serum Zinc ◽  
Feed Consumption ◽  
Canola Meal ◽  
Lower Serum ◽  
Protein Supplements ◽  
Growing Period ◽  
Specific Pathogen ◽  
Daily Gain

A factorial experiment with four dietary levels of supplemental lysine (0.10, 0.15, 0.20, 0.25%), three protein supplements (soybean meal (SBM), canola meal (CM), CM + 50 mg iodinated casein per kg diet), and two sexes of pigs (barrows, gilts) was conducted. Crossbred Lacombe × (Yorkshire × Landrace), specific pathogen-free pigs were randomly allotted at 23 ± 1 kg weight in four replicates to pens of four pigs, individually fed. The pigs were slaughtered at 100 ± 2 kg. The diets were based on barley and wheat (2:1) and were pelleted (4.8 mm diam.). Average daily gain (ADG) responses during the growing period (23–57 kg) to increments of lysine were not significant (P > 0.05) but when ADG was adjusted by regression for variation in feed consumption there was a significant (P < 0.05) linear response (743, 748, 757, 772 g d−1). Feed:gain ratio also showed improvement (P < 0.05): 2.45, 2.44, 2.40, 2.35. Responses to lysine were similar for SBM and CM diets but pigs fed SBM had greater (P < 0.05) ADG than those fed CM (779 vs. 745 g). There was no response to added lysine in the 57–100 kg weight range but pigs fed SBM had greater adjusted ADG than those fed CM (908 vs. 873 g) and better feed:gain ratio (3.33 vs. 3.46). Thyroid status was not affected but iodinated casein improved grower period feed:gain ratio. Carcass quality index was not affected (P > 0.05) by lysine level, protein source, iodinated casein or sex of pig. Pigs fed CM had lower serum zinc than those fed SBM. It was estimated that the addition of 0.25% lysine to diets containing 0.8% lysine resulted in 7% improvement in ADG and efficiency of feed utilization. Key words: Lysine, canola meal, zinc, thyroid, pigs

Validation of the cannulated pig model for determining intestinal nutrient disappearance in cattle

2005 ◽  
Vol 85 (1) ◽  
pp. 85-91
Author(s):  
D. M. Loveday ◽  
P. A. Thacker ◽  
D. G. Wilson ◽  
J. J. McKinnon
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Distillers Grains ◽  
Pig Model ◽  
Barley Straw ◽  
Canola Meal ◽  
Regression Equations ◽  
Protein Supplements ◽  
Barley Silage ◽  
Feed Samples

The mobile nylon bag technique was used to determine the potential of cannulated pigs to model intestinal dry matter (DM) and crude protein (CP) digestibility of ruminal undegradable residues (RUDR) of various feed samples in cattle. A total of 11 feeds (six protein supplements and five forages) varying in rumen degradability were utilized: canola meal (regular vs. heated); soybean meal (regular vs. heated); distillers’ grains (regular vs. heated ); alfalfa hay (regular vs. dehydrated alfalfa pellets); barley silage (regular vs. heated); and barley straw. Ruminal undegradable residues of each feedstuff were placed into eight polyester bags and inserted into the duodenum of two cannulated Hereford steers (644 ± 14 kg). Another eight bags for each RUDR sample were randomly allocated to six crossbred gilts (Camborough 15 line female × Canabred sire; 50 ± 1.2 kg body weight) and inserted into the pigs’ duodenum via duodenal cannulas. Bags were recovered in the feces from both the cattle and pigs. Regression analysis was used to relate intestinal DM and CP digestibility obtained in pigs with those obtained in steers. Intercepts not different from zero were forced through the zero intercept to obtain a measure of bias and loss of precision resulting from forcing the zero intercept. The non-zero regression equations relating steer estimates to those obtained from pigs were -3.17 + 0.95 X [r2 = 0.98, residual standard deviation (RSD) = 3.84] and 0.35 + 0.96 X (r2 = 0.98, RSD = 6.16) for DM and CP disappearance, respectively. Forcing the non-zero intercept resulted in pigs over predicting intestinal DM disappearance in cattle by 10.6% with a RSD of 4.30. Using the pig model, intestinal CP disappearance of cattle was over predicted by 3.1% with a RSD of 5.85. It was concluded that pigs can be used to model intestinal DM and CP disappearance of ruminal undegraded feed residues in cattle. Key words: Cattle, pigs, mobile nylon bag technique, intestinal nutrient availability

scholarly journals PSXI-15 Effects of post-pyrolysis treated biochars on nutrient disappearance, methane production and ruminal fermentation of a silage-based diet in an artificial rumen system (RUSITEC)

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 395-395
Author(s):  
Paul Tamayao ◽  
Gabriel O Ribeiro ◽  
Tim A McAllister ◽  
Hee-Eun Yang ◽  
A M Saleem ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Block Design ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Canola Meal ◽  
Acid Mixture ◽  
Vitamin Supplement ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Barley Silage

Abstract This study investigated the effects of post-pyrolysis treated biochar on nutrient disappearance, total gas and methane (CH4) production, rumen fermentation and microbial protein synthesis in an artificial rumen system (RUSITEC) fed a barley silage-based diet. The basal diet consisted of 60% barley silage, 27% barley grain, 10% canola meal and 3% mineral/vitamin supplement (DM basis). Three spruced-based biochars, treated post-pyrolysis with either zinc chloride, hydrochloric acid/nitric acid mixture or sulfuric acid were added at 2.0% of substrate DM. In a randomized complete block design, treatments were assigned to sixteen vessels (n = 4/treatment) in two RUSITEC systems. The experiment was conducted over 15 d, with 8 d of adaptation and 7 d of sampling. Nutrient disappearance of dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) was determined after 48 h of incubation from d 9 to 12, and microbial protein synthesis was measured from d 13–15. Data were analyzed using PROC MIXED in SAS, with the fixed effect of treatment and random effect of RUSITEC system and vessel. Biochar inclusion did not affect disappearance of DM (P = 0.49), OM (P = 0.60), CP (P = 0.14), NDF (P = 0.48), ADF (P = 0.11) or starch (P = 0.58). Biochar also had no effect on total gas production (P = 0.31) or CH4 produced expressed as a % of total gas production (P = 0.06), mg/d (P = 0.70), mg/g of DM incubated (P = 0.74), or mg/g of DM digested (P = 0.64). No effect on total VFA (P = 0.56) or NH3-N (P = 0.20) production were observed. Neither microbial protein synthesis nor total protozoa count were affected by biochar addition (P &gt; 0.05). In conclusion, biochar inclusion in a silage-based diet did not exhibit the potential to mitigate CH4 emissions or improve digestion in a RUSITEC system.

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