The potential of isobutyraldehyde monourea (propanal, 2-methyl-monourea) as a nonprotein nitrogen source for ruminant animals

1994 ◽  
Vol 74 (4) ◽  
pp. 665-674
Author(s):  
G. W. Mathison ◽  
R. Soofi-Siawash ◽  
M. Worsley

Experiments were earned out with sheep and in vitro to evaluate isobutyraldehyde monourea (IBMU; propanal-2-methyl-monourea) as a slow-release nitrogen source for ruminant animals. Examination of the compound for carbon, hydrogen, and nitrogen composition and infrared, NMR and mass spectral analyses indicated that IBMU was present in significant amounts. Ten sheep were randomly assigned to diets containing bromegrass hay and corn grain and the following nitrogen supplementation treatments: (1) control, (2) urea, (3) soybean meal, (4) canola meal and (5) IBMU, with the protein supplements providing approximately 25% of the nitrogen intake. Similar treatments were also prepared and evaluated in vitro, with the exception that wheat straw rather than bromegrass hay was used in the incubation. The digestibility of nitrogen in the ration containing IBMU was higher (P < 0.05) than its digestibility in the other rations when measurements were conducted with sheep over three 14-d periods. No differences in dry matter (DM) or energy digestibility were detected between the different treatments. The digestible energy content of IBMU was estimated to be greater than 21 MJ kg−1. The digestibilities of DM in vitro after 2, 4, 8, 12, 24, 48 and 72 h of incubation were not influenced by the presence of IBMU. No influence of nitrogen supplementation on nitrogen balance was observed, probably because of the high protein content of the control ration (10.6%). IBMU exhibited the characteristics of an ideal slow-release nonprotein nitrogen supplement, since concentrations of rumen ammonia nitrogen did not vary from 0 to 8 h after feeding and ammonia concentrations were higher (P < 0.05) both before feeding and 8 h after feeding when IBMU was fed than when any of the other supplements were fed. The concentration of isobutyric acid averaged 2.6-fold higher when IBMU was fed than when canola meal or soybean meal was fed, confirming that most of the compound was degraded in the rumen. Sheep fed IBMU had lower concentrations of acetic, propionic and butyric acid in the rumen than those fed natural protein supplements. It was unclear whether the decrease in VFA concentration was positive or negative in terms of nutritional value. It was concluded that IBMU exhibited some ideal characteristics for a nonprotein nitrogen supplement for ruminant animals because it was degraded at an optimum rate in the rumen and provided rumen microflora with isobutyric acid. Further experimentation is warranted to determine its usefulness when nitrogen-deficient diets are fed to ruminant animals. Key words: Nonprotein nitrogen, isobutyric acid, isobutyraldehyde, slow-release, isobutyraldehyde monourea, propanal-2-methyl-monourea

1995 ◽  
Vol 75 (3) ◽  
pp. 371-377 ◽  
Author(s):  
K. Stanford ◽  
T. A. McAllister ◽  
Z. Xu ◽  
K.-J. Cheng ◽  
M. Pickard

An experiment was undertaken to determine the effect of lignosulfonate (LSO3) treatment on the rumen-undegradable protein (RUDP) value of canola meal (CM) and soybean meal (SM). Canola meal and SM were treated with 7% LSO3 and heated (95 °C) for 1 h. Treated and untreated meals were compared in vitro, in situ and in digestibility and growth experiments using Romanov × Suffolk and Romanov × Dorset lambs. Lambs were fed four barley-based diets [13.5% crude protein (CP)] with one of CM, SM, LSO3-treated CM (LSO3-CM) or LSO3-treated SM (LSO3-SM) as protein supplements. Lignosulfonate treatment increased acid detergent insoluble nitrogen (ADIN) five-fold in SM and threefold in CM. After 6 h incubation with ruminal fluid, ammonia production from LSO3-treated meals was one-half that from untreated meals. Lignosulfonate treatment reduced (P < 0.01) in situ rates of dry matter (DM) and protein disappearance and the soluble DM and protein fractions for both SM and CM, but not the potentially digestible fractions. No differences in feed conversion or average daily gain (P > 0.05) were found among the treatments. Although LSO3 treatment did not improve the ADG of lambs fed barley-based diets, it increased the RUDP value of SM and CM without adverse effects on digestibility. Key words: Lignosulfonate, canola meal, soybean meal, lamb


2017 ◽  
Vol 1 (4) ◽  
pp. 592-598 ◽  
Author(s):  
J. M. Lourenço ◽  
M. A. Froetschel ◽  
J. R. Segers ◽  
J. J. Tucker ◽  
R. L. Stewart

Abstract Two experiments were conducted to evaluate 3 silage-based stocker diets. In Exp. 1, diets were fed to a total of 276 animals over a period of 3 yr and performance data was collected. In Exp. 2, the same diets were subjected to in vitro digestion for 5 time periods: 0, 6, 12, 24, and 48 h, to evaluate IVDMD, production of fermentation end products, and efficiency of transformation of energy. The experimental diets were similar, except for their protein supplements. They were composed of: 1) 74% corn silage, 15.2% ground ear corn, and 10.8% soybean meal (SBM); 2) 74.4% corn silage, 9.8% ground ear corn, and 15.8% canola meal (CAN); 3) 74.5% corn silage, 9.8% ground ear corn, and 15.7% sunflower meal (SUN). Results from Exp. 1 showed that DMI was similar across all treatments (P = 0.167), but ADG was greater (P = 0.007) for animals fed either SBM or CAN than for animals fed SUN (1.29, 1.28, and 1.20 kg/d, respectively). Both CAN and SUN significantly reduced (P &lt; 0.001) daily feeding cost per animal in comparison to SBM. Exp. 2 revealed that total VFA production was similar for all treatments (P = 0.185), and greatest molar proportions of propionate were observed for SBM and CAN (P = 0.02). Additionally, IVDMD was highest for SBM (P &lt; 0.001). Regression analysis showed that most of the evaluated traits followed a quadratic trend for incubation times (P ≤ 0.02). On average, the in vitro technique used in this study was able to account for 97.03% of the caloric transformations suffered by DE throughout the different incubation times. Overall, our findings revealed that although animals receiving SUN had the cheapest daily feeding cost, important traits like ADG and feed conversion rate were negatively affected by this treatment. In contrast, data showed that CAN was an effective replacement for SBM for it maintained similar animal performance while decreasing feed costs. Therefore, from a producer standpoint, CAN is a viable alternative to replace the more costly SBM diet in silage-based stocker operations.


2019 ◽  
Vol 64 (No. 7) ◽  
pp. 294-301
Author(s):  
S Gonzalez-Munoz ◽  
J Sanchez ◽  
S Lopez-Aguirre ◽  
J Vicente ◽  
J Pinos-Rodriguez

One in vitro assay and one in vivo trial with ruminally cannulated Holstein steers were conducted to evaluate the effects of a dietary substitution of soybean meal by a urea and slow-release urea source of fermentation and degradation of diets for cattle. The experimental diets consisted of the total mixed rations defined as the control with soybean meal (SBM), U (urea), SRU (slow-release urea), and SRU+U+AA (0.42% + 0.42% + 1% amino acids methionine and lysine). The dietary substitution of SBM by U or SRU reduced (P &lt; 0.05) the total gas production (V), microbial mass and degradation at 72 h incubation under the in vitro conditions, as well as the degradation rate (c) and the total volatile fatty acids (VFA) in the rumen of the steers; however, when the dietary substitution of SBM was by U+SRU+AA, those values did not decrease. In the steers, the dietary substitution of SBM by U and SRU reduced the ruminal degradation rate and the total VFA, and increased the ammonia N, but when SBM was substituted by U+SRU+AA in the diets, these changes were not observed. No advantage of SRU over U was found. The dietary substitution of SBM by U, SRU, U+SRU+AA did not modify the molar proportion of the VFA in the rumen nor were there changes in the nutrient digestion or excretion. Both the in vitro assay and the in vivo trial indicated that replacing SBM with U or SRU increases the ruminal ammonia N concentrations and reduces the degradation rate in the rumen, although those undesirable findings were not found when the SBM was replaced by U+SRU+AA. Therefore, it is feasible to replace the SBM with a combination of urea, slow-release urea, lysine and methionine in the diet for the ruminants.


1999 ◽  
Vol 50 (4) ◽  
pp. 497 ◽  
Author(s):  
D. G. Masters ◽  
G. Mata ◽  
S. M. Liu

There is limited evidence that the response in wool growth resulting from feeding protected protein supplements continues after the feeding has stopped. Feeding such proteins, alternated with traditional supplements, may increase wool growth as much as continuous feeding but at a lower cost. This experiment aimed to determine whether the response to protected protein continued after the sheep were switched to a cereal supplement. Over a 2-month experimental period, 56 weaners (5 months old, weighing 26 kg) were used in a 2 × 2 factorial experiment. Half were fed a diet containing 25% canola meal [partially protected protein with high concentrations of sulfur amino acids (SAA)] mixed with oaten hay, urea, and minerals. The other half were fed the same diet but with lupin seed (highly degradable protein with low concentrations of SAA) replacing the canola meal. Within each of the 2 dietary treatments and in each of 2 months, half of the weaners were fed the diet continuously, the other half were fed the diet for 2 weeks followed by 2 weeks of a barley, oats, hay, urea, and minerals diet. Another group of 8 weaners was fed the oats–barley diet continuously for 2 months. All sheep were fed to lose 35 g liveweight/day. Weaners fed canola meal grew 11% more wool during the experiment and had a higher rate of protein synthesis in the skin than weaners fed lupins. The response to canola meal of wool and skin was the same whether feeding was continuous or alternated with oats–barley, indicating that the benefits from feeding partially protected proteins continues after feeding has stopped.


1988 ◽  
Vol 68 (2) ◽  
pp. 523-532 ◽  
Author(s):  
R. MISIR ◽  
R. BLAIR

The biological availability of biotin in canola meal (CM), cereal grain, cereal grain plus CM, and soybean meal (SBM) was estimated, using 140 pigs in the 10- to 20-kg weight range. In exp. I, pigs (six/treatment) were individually housed and fed a basal casein-cornstarch diet with 0, 50, 100, 150, 200 or 250 μg d-biotin kg−1 to generate data for a dose-response curve based on plasma biotin concentration. Other pigs were fed CM, SBM or soyprotein isolate (SPI) included in the diet. In exp. II, pigs were housed in pairs and four pairs were fed diets based on cereal grain or cereal grain plus CM. Results (exp. I) showed a higher correlation between daily biotin intake and plasma biotin (r = 0.835, P < 0.01) than on growth rate (r = 0.627, P < 0.05). The regression for plasma biotin (Y) on biotin intake (X) computed from the linear portion of the curve (i.e., 0-200 μg added biotin kg−1) was[Formula: see text]Biotin bioavailability in various feedstuffs was highest for corn (101.2%), followed by SBM (85.5%), CM (70.9%), and wheat (33.3%), triticale (25.9%), sorghum (25.1%) and barley (24.0%). Key words: Biotin bioavailability, protein supplements, cereal grains, swine


1984 ◽  
Vol 64 (1) ◽  
pp. 159-164 ◽  
Author(s):  
F. G. PROUDFOOT ◽  
H. W. HULAN ◽  
K. B. McRAE

Three experiments involving 11 600 male broiler chickens sought to determine if the pelleting process affects the dietary micronutrient, fat and protein components to increase the incidence of sudden death syndrome. Processed dietary micronutrient and fat components were not significantly associated with an increase in sudden death syndrome among broiler chickens. In one of the experiments, the incidence of sudden death syndrome was reduced (P < 0.01) when the dietary protein supplements (soybean meal, canola meal and fishmeal) bypassed the pelleting process. Key words: Sudden death syndrome, broiler chickens, feed pelleting, fat, micronutrients, protein supplements


1991 ◽  
Vol 71 (2) ◽  
pp. 497-506 ◽  
Author(s):  
J. M. Bell ◽  
M. O. Keith ◽  
D. S. Hutcheson

Two very low glucosinolate (VLG) canola meals from crop years 1987 and 1988 (CM; 1.66 and 0.53 μmol g−1) and two Tobin CM (B. campestris) (10.71 and 15.62 μmol g−1) were evaluated in experiments with pigs. In exp. 1, 1987 Tobin CM, VLG-CM and soybean meal (SBM) were compared in individually fed meal and pelleted diets. Daily gains from 23 to 57 kg were similar for the CMs (VLG-CM 662 g, Tobin CM 645 g) but lower than those obtained with SBM (730 g; P < 0.01). Daily feed intakes of pigs fed CM were lower than for those fed SBM (P < 0.05). Plasma thyroxine (T4) levels were similar for SBM and VLG-CM and were above those from pigs fed Tobin (P < 0.05) CM. Triiodothyronine (T3) levels were lower for VLG-CM than for SBM (P < 0.05) and the T3 value for Tobin CM was intermediate. Pelleting enhanced T3 (0.70 vs 0.94 ng mL−1) and T4 (50 vs. 60 ng mL−1) levels with all protein supplements (P < 0.01). In exp. 2, similar diets were fed ad libitum. Daily gains for SBM were greater than for Tobin CM (803 vs. 744, P < 0.05) and gains for pigs fed VLG-CM was 774 g. Daily feed intakes were similar (2.01, 1.99 and 1.92 kg). In exp. 3 energy digestibility of 1987 VLG-CM was greater (P < 0.01) than for Tobin CM (75.0 vs 67.4% for 30-kg pigs). Apparent digestibility coefficients of crude protein (CP) were 71.9, 71.2 and 71.6 in 16% CP diets. In exp. 4 Tobin and VLG-CMs from 1987 and 1988 crops were compared with SBM and commercial CM using 96 pigs (48M, 48F). Daily gains and feed intakes were similar for the CMs but T3 and T4 values were below those of SBM (P < 0.05). Key words: Canola meal, very low glucosinolate, digestibility, feeding value, pigs


2018 ◽  
Vol 19 (2) ◽  
pp. 119
Author(s):  
Efka Aris Rimbawanto ◽  
Sri Suhermiyati ◽  
Bambang Hartoyo

The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis.


1994 ◽  
Vol 74 (3) ◽  
pp. 479-485 ◽  
Author(s):  
G. R. Khorasani ◽  
J. J. Kennelly ◽  
P. H. Robinson

The objectives of the present study were to evaluate the nutritive value of expeller linseed meal (ELM) and solvent linseed meal (SLM) as protein supplements for lactating dairy cows utilizing analytical and in situ procedures, and determine the in vivo nutritive value of the most promising linseed meal in diets containing either barley or corn-based concentrate. Canola meal (CM) and soybean meal (SBM) were included in the evaluative stage for comparative purposes. Linseed meals contained 37.8 and 35.4% crude protein (CP); 22.6 and 25.9% neutral detergent fibre (NDF); and 12.9 and 7.1% crude fat for ELM and SLM, respectively. Rate and extent of dry matter, NDF, and CP digestion, as determined by the rumen in situ technique, indicated that ruminal digestibility of SLM was similar to that observed for CM, but ruminal escape of ELM protein was higher than for CM. The proportion of ruminal escape protein was similar for SLM, CM and SBM. Total tract CP digestibilities of SLM and CM were similar to and lower than those observed for ELM and SBM. Soybean meal had the highest total tract CP digestibility. Based on results of the analytical and in situ procedures, SLM was selected for evaluation in the in vivo experiment. Results indicated that SLM did not have any deleterious effect on digestion of other components of the diet, and parameters of rumen fermentation indicated similar digestion patterns for cows fed SLM and CM. Overall, results indicate that SLM has potential as a replacement for CM or SBM in diets of dairy cattle. It was also concluded that replacement of barley with corn does not influence the nutritive value of SLM for dairy cows. Key words: Linseed meal, canola meal, dairy cattle


2019 ◽  
Vol 46 (1) ◽  
pp. 236-245
Author(s):  
A. Y. Abdullahi ◽  
A. S. Muhammad ◽  
M. H. Zango ◽  
S. Madaki ◽  
M. A. Dambatta ◽  
...  

An experiment was conducted to assess the appearance, smell, texture, pH, temperature, chemical composition and energy content of ensiled Rice offal (RO) treated with non-protein nitrogen sources and soybean meal residue in a completely randomized design. Rice offal was treated with different proportions of Urea, Poultry litter (PL) and Soybean meal residue (SBMR) and ensiled. The treatment combinations were; A (100%RO), B (100%RO + Urea), C (75%RO + 25%PL), D (50%RO + 50%PL), E (75%RO + 25%SBMR) and F (50%RO + 50%SBMR). The treatments were ensiled for 21 days in triplicates. The results showed that silages were adequately fermented with sweet aroma. The silages were acidic (4.70 – 5.80) except for treatments C and D which had pH of 7.20 and 7.00, respectively. The temperatures of the silages were significantly (P<0.05) different. The proximate composition of silages differed significantly (P<0.05), being highest for %CP, %EE and ash in treatment B. Dry matter contents were also significantly different (P<0.05) with treatment A having the highest (90.25%). Rice offal and Urea mixtures resulted in silages with highest NDF, HEM and Energy. Lowest NDF was obtained in treatment without additive (Treatment A) compared to all other treatments. Least ADF was obtained in Treatment E (75% RO + 25% SBMR). Enhancement of rice offal with urea, poultry litter and soybean meal residue has shown improvement in the silage physiochemical quality


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