Effect of soybean roasting and monensin on microbial protein synthesis, ruminal parameters and plasma metabolites of lactating dairy cows

2015 ◽  
Vol 55 (5) ◽  
pp. 625
Author(s):  
E. Abdi ◽  
F. Fatahnia ◽  
M. Dehghan Banadaki ◽  
A. Azarfar ◽  
S. G. Mosavi
Keyword(s):  
Plasma Concentration ◽  
Dairy Cows ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Soybean Seed ◽  
Plasma Metabolites ◽  
Microbial Protein ◽  
Lactating Dairy Cows ◽  
Dietary Treatments

This study was conducted to evaluate the effects of inclusion of roasted whole soybean seed and monensin (MO) in the diets of lactating dairy cows on plasma metabolites, ruminal parameters, and microbial protein synthesised in the rumen. Four multiparous Holstein lactating dairy cows (third parity; 656 ± 55 kg of liveweight; 83 ± 10 days in milk; 35 ± 4 kg/day milk yield) were assigned to a balanced 4 × 4 Latin square design. Each experimental period lasted 21 days with 14 days of treatment adaptation and 7 days of data collection. The control diet (C) was a total mixed ration consisting of 40% forage and 60% concentrate mixture on a dry matter (DM) basis. These cows were randomly assigned to one of the four dietary treatments. The first treatment was the C diet of unprocessed whole soybean seed, second was the C diet supplemented with 24 mg of MO/kg of DM (M), the third was roasted whole soybean seed (R) and the fourth treatment was R diet supplemented with 24 mg of MO/kg of DM (RM). Urinary excretion of creatinine and purine derivatives, microbial protein synthesised in the rumen, rumen pH and rumen concentrations of volatile fatty acids and ammonia were similar among the dietary treatments (P > 0.05). Orthogonal contrasts showed that the rumen concentration of acetate was lower in MO-supplemented cows than non-supplemented cows (P < 0.05). Dietary treatments had no effects on plasma concentrations of glucose, total cholesterol, triglycerides and total protein (P > 0.05). Plasma concentration of urea was significantly lower in cows fed with the RM diet compared with cows fed the C and M diets (P < 0.05). In conclusion, dietary treatments had no effect on microbial protein synthesised in the rumen, plasma metabolites (except for plasma concentration of urea) and ruminal parameters of dairy cows.

Intraruminal administration ofMegasphaera elsdeniimodulated rumen fermentation profile in mid-lactation dairy cows

2011 ◽  
Vol 79 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Qendrim Zebeli ◽  
Sarah J Terrill ◽  
Alberto Mazzolari ◽  
Suzanna M Dunn ◽  
Wen Z Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Plasma Concentrations ◽  
Rumen Fermentation ◽  
Holstein Cows ◽  
Plasma Metabolites ◽  
Fermentation Profile

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

Utilisation of white clover silage fed alone or with maize silage by lactating dairy cows

1999 ◽  
Vol 39 (3) ◽  
pp. 237 ◽  
Author(s):  
D. E. Auldist ◽  
K. L. Atkinson ◽  
D. W. Dellow ◽  
M. J. Silvapulle ◽  
G. H. McDowell
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
White Clover ◽  
Volatile Fatty Acids ◽  
Plasma Concentrations ◽  
Mixed Diet ◽  
Maize Silage ◽  
Dietary Nitrogen ◽  
Lactating Dairy Cows ◽  
High Yields

Six lactating dairy cows in mid lactation were fed either white clover silage or a mixture of white clover and maize silages (70 : 30, dry matter basis). Milk yields (about 20 L/day) and compositions were similar when cows were fed either diet. Although the mixed diet was less digestible and provided less energy and crude protein than white clover, rumen fermentation may have been improved relative to that when white clover silage was fed alone. Flow rates of fluid from the rumen were faster, pH values for rumen liquor were higher and concentrations of ammonia in rumen liquor were lower when cows were fed the mixed diet than white clover silage. Concentrations of total volatile fatty acids and the proportions of the major volatile fatty acids in rumen liquor were similar for both diets. The efficiency of utilisation of dietary nitrogen for liveweight gain and milk production also appeared to be similar for both diets. Plasma concentrations of key metabolites were consistent with the cows being well nourished when fed both diets. Overall, it was evident that white clover silage may be used effectively in combination with lower quality maize silage to meet the nutrient requirements of cows with reasonably high yields of milk during mid lactation.

scholarly journals Performance of Lactating Dairy Cows Fed Dried Sardines

1999 ◽  
Vol 4 (1) ◽  
pp. 35 ◽  
Author(s):  
A.S. AI-Abri ◽  
C.D. Lu ◽  
R.J. Early ◽  
A. Srikandakumar ◽  
O. Mahgoub ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Experimental Period ◽  
Milk Composition ◽  
Ruminal Ph ◽  
Lactating Dairy Cows ◽  
Continuous Feeding

To utilize locally available feed resources with livestock production in hot climates, dried sardines were incorporated into diets for lactating dairy cows. Fourteen Holstein and 13 Australian Milk Zebu multiparous cows were used in a 70-day continuous feeding experiment. lsonitrogenous and isoenergetic diets containing either soybean meal or dried sardines (supplied 40% of dietary crude protein) were fed ad - libitum. Comparisons between diets were made during the 7-week experimental period. The experiment was conducted as a 2 x 2 factorial arrangement of treatment, with diet and breed as main factors. Ruminal pH, ammonia N, total and individual volatile fatty acids concentrations were not altered by the feeding of dried sardines. Intakes of dry matter, energy, crude protein, and acid detergent fiber were lower (P<0.05) in both breeds of cows fed dried sardines. Intakes of ether extract and ash were higher (P<0.05) in cows fed dried sardines. Although it was not statistically significant, milk production was higher (P>0.05) in Holstein cows fed dried sardines than those fed the control diet (16.2 vs 15.1 kg/d). Feeding of dried sardines did not affect milk composition and compositional yields. Milk production was higher (P<0.01) in Holstein than Australian Milk Zebu cows. Effect of diet and breed interaction on milk production was significant (P<0.01 ). Potential of feeding marine proteins may be higher for higher milk producers (Holstein) than lower producers (Australian Milk Zebu). Reproduction parameters, body condition scores, and heat-stress associated parameters were not affected by the feeding of dried sardines. This study suggests that dried sardines could be incorporated into diets of lactating dairy cows without affecting milk production.

The effect of asynchronous diets on the function of the rumen in the lactating dairy cow

1998 ◽  
Vol 1998 ◽  
pp. 19-19
Author(s):  
A.R. Henderson ◽  
P.C. Garnsworthy ◽  
J.R. Newbold ◽  
P.J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Production Performance ◽  
Early Lactation ◽  
Microbial Protein ◽  
Nutrient Flows ◽  
Lactating Dairy Cows ◽  
Overall Efficiency ◽  
Fermentation Parameters

Sinclair et al. (1993) found that a diet formulated to be synchronous with regard to hourly release of nitrogen and energy increased microbial protein synthesis by 14% in sheep. Dairy cows in early lactation experience a shortfall of energy and protein, with available protein determining the overall efficiency of metabolism (MacRae and Lobley, 1986) and subsequent milk production. It is therefore necessary to maximise microbial protein yield during this period. In this study diets were designed for lactating dairy cows to contain the same feed ingredients, but to release nitrogen and energy in the rumen at different times. Rumen fermentation parameters, nutrient flows to the small intestine and production performance were investigated.

scholarly journals Effect of Different Tannin Sources on Nutrient Intake, Digestibility, Performance, Nitrogen Utilization, and Blood Parameters in Dairy Cows

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 507 ◽  
Author(s):  
Zhang ◽  
Xu ◽  
Cao ◽  
Wang ◽  
Yang ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Nutrient Intake ◽  
Control Diet ◽  
Nitrogen Retention ◽  
Blood Parameters ◽  
Nitrogen Utilization ◽  
Utilization Efficiency ◽  
Nitrogen Excretion ◽  
Nitrogen Utilization Efficiency ◽  
Dietary Treatments

This study investigated the effect of tannin sources on nutrient intake, digestibility, performance, nitrogen utilization, and blood parameters in lactating dairy cows. Four multiparous lactating Holstein cows were used in a balanced 4 × 4 Latin square design, with each period lasting 28 days. Cows were randomly assigned to one of four dietary treatments: Control diet (CON, a totally mixed ration without tannin supplements), control diet supplemented with 3% bayberry condensed tannins (BCT), control diet supplemented with 3% Acacia mangium condensed tannins (ACT), and control diet supplemented with 3% valonia hydrolyzed tannins (VHT). Dietary treatments did not significantly affect nutrient intake, milk yield or composition, microbial protein synthesis, nitrogen utilization efficiency, or plasma concentrations of glucose, non-esterified fatty acids, β-hydroxybutyrate, total protein, and globulin, or the albumin-to-globulin ratio. Tannin supplements decreased the apparent total tract nutrient digestibility to varying degrees and significantly decreased the milk and blood urea nitrogen contents (p < 0.05). Tannin supplements altered nitrogen excretion routes in lactating dairy cows, and BCT significantly decreased the urinary nitrogen excretion (p = 0.04). Compared with the CON, ACT, and VHT diets, BCT yielded the highest nitrogen retention and nitrogen retention-to-digestible nitrogen ratio despite having a similar nitrogen utilization efficiency (p < 0.05). Bayberry condensed tannin supplementation may be a potential way to improve nitrogen utilization and reduce concerns regarding nitrogen excretion in dairy cows.

scholarly journals Feeding Agroindustrial Byproducts to Light Lambs: Influence on Growth Performance, Diet Digestibility, Nitrogen Balance, Ruminal Fermentation, and Plasma Metabolites

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 600 ◽  
Author(s):  
Trinidad de Evan ◽  
Almudena Cabezas ◽  
Jesús de la Fuente ◽  
María Dolores Carro
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Animal Health ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Barley Straw ◽  
Plasma Metabolites ◽  
Ruminal Fermentation ◽  
Feed Conversion ◽  
Homogeneous Groups

The objective of this study was to evaluate the effect of replacing cereals and protein concentrates in a high-cereal concentrate (control) for light lambs with corn distiller’s dried grains with solubles (DDGS; 18%), dried citrus pulp (DCP; 18%), and exhausted olive cake (EOC; 8%) in a byproduct (BYP) concentrate on growth performance, digestibility, ruminal fermentation, and plasma metabolites. Two homogeneous groups of Lacaune lambs (13.8 kg ± 0.25 kg) were fed one of each concentrates and barley straw ad libitum until reaching about 26 kg body weight. There were no differences between groups on feed intake, average daily gain, or feed conversion ratio, but the control diet had greater (p < 0.001) dry matter digestibility. Diet had no effect on post-mortem ruminal pH and total volatile fatty acid concentrations and profile, but NH3-N concentrations were lower (p = 0.003) for the BYP-fed group compared with the control one. However, plasma concentrations of amino acids, total proteins, urea, and hepatic enzymes were not affected by the diet. In conclusion, 44% of feed ingredients in the concentrate for light lambs can be replaced with a mixture of corn DDGS, DCP, and EOC without negatively affecting growing performance and animal health.

scholarly journals Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume

1993 ◽  
Vol 69 (2) ◽  
pp. 385-396 ◽  
Author(s):  
Jan Dijkstra ◽  
Huug Boer ◽  
Jaap Van Bruchem ◽  
Marianne Bruining ◽  
Seerp Tamminga
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Acid Concentration ◽  
Butyric Acid ◽  
Absorption Rate ◽  
Volatile Fatty Acids ◽  
Liquid Volume ◽  
Lactating Dairy Cows ◽  
Volatile Fatty Acid Concentration ◽  
Fractional Absorption

The effect of rumen liquid volume, pH and concentration of volatile fatty acids (VFA) on the rates of absorption of acetic, propionic and butyric acids from the rumen was examined in lactating dairy cows. Experimental solutions introduced into the emptied, washed rumen comprised two different volumes (10 or 30 1), four levels of pH (4.5, 5.4, 6.3, 7.2) and three levels of individual VFA concentrations (20, 50 or 100 mM-acetic, propionic or butyric acid). All solutions contained a total of 170 mM-VFA and an osmotic value of 400 mOsmol/l. Absorption rates were calculated from the disappearance of VFA from the rumen corrected for passage with liquid phase to the omasum. An increase in initial fluid pH caused a reduction in fractional absorption rates of propionic and butyric acids. Increasing the initial pH from 4.5 to 7.2 reduced fractional absorption rates of acetic, propionic and butyric acids from 0.35, 0.67 and 0.85 to 0.21, 0.35 and 0.28/h respectively. The fractional absorption rates of all VFA were reduced (P < 0.05) by an increase in initial rumen volume. The fractional absorption rate of acetic acid was lower (P < 0.05) at an initial concentration of 20 mM than of 50 mM. The fractional absorption rate of propionic acid tended (P < 0.10) to decrease as the level of concentration increased while fractional absorption rate of butyric acid was not affected by butyric acid concentration. These results indicate that relative concentrations of VFA in rumen fluid might not represent relative production rates and that attempts to estimate individual VFA production from substrate digestion must take account of pH and VFA concentration.

Sign in / Sign up

Export Citation Format

Share Document