The nutritive value of subterranean clover herbage grown under irrigation in northern Victoria

1992 ◽  
Vol 43 (6) ◽  
pp. 1265 ◽  
Author(s):  
CR Stockdale
Keyword(s):  
Amino Acid ◽  
Chemical Composition ◽  
Nitrogen Content ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Acid Detergent Fibre ◽  
Amino Acid Profiles ◽  
Series Of Experiments

A series of experiments is described which considered the nutritive value of subterranean clover (Trifolium subterraneum) herbage grown under irrigation in northern Victoria. Digestion studies were undertaken with sheep and dairy cows and the degradation of herbage samples in nylon bags was evaluated. Digestion coefficients were estimated, 1eaf:stem ratios were measured, mineral and amino acid profiles were obtained, and protein degradabilities were assessed. The digestibility and chemical composition of freshly cut subterranean clover herbage was relatively constant until wilting of the herbage in mid spring. With few exceptions, in vivo DM digestibility and nitrogen content were greater than 75% and 3.2%, respectively, and neutral detergent fibre, acid detergent fibre and lignin were less than 40%, 30% and 6%, respectively. The chemical composition of leaves and petioles/stems were very different. The digestibility of the leaf fraction was often much lower than that of petiole/stem, but its nitrogen content was higher. Reasons for this, and their implications, are discussed. Discussed also are the adequacy of herbage minerals and their solubilities. Rumen protein degradability for vegetative herbage ranged from 0.51 to 0.64. Of the plant components, the protein in stems was more degradable than the protein in leaves. The effects of incubation of herbage in nylon bags in the rumen for 24 h on amino acid composition are also discussed.

The nutritive value of Persian clover (Trifolium resupinatum) herbage grown under irrigation in northern Victoria

1993 ◽  
Vol 44 (7) ◽  
pp. 1557 ◽  
Author(s):  
CR Stockdale
Keyword(s):  
Chemical Composition ◽  
Nitrogen Content ◽  
Dry Matter ◽  
Nutritive Value ◽  
Acid Detergent Fibre ◽  
Amino Acid Profiles ◽  
Series Of Experiments ◽  
Persian Clover ◽  
Trifolium Resupinatum

A series of experiments is described which considered the nutritive value of Persian clover (Trifolium resupinatum) (cv. Maral) herbage grown under irrigation in northern Victoria. Digestion studies were undertaken with sheep and dairy cows, and the degradation of herbage samples in nylon bags was evaluated. Digestion coefficients were estimated, leaf and stem characteristics were measured, mineral and amino acid profiles were obtained, and protein degradabilities were assessed throughout the period of growth of Persian clover. Freshly harvested Persian clover herbage utilized before flowering appears to be an excellent feedstuff for ruminants, and its chemical composition remains relatively constant until late spring. In vivo OM digestibilities were in the range, 75-85%, although this depended on feeding level and time of the year. When vegetative, nitrogen content ranged from 3.0 to 4.6%, and neutral and acid detergent fibre levels were 24-34 and 21-27%, respectively. Acid detergent lignin concentrations were between 2.1 and 4.5% of dry matter. Dry matter contents of the herbage were often very low; this could influence intake and digestion. The chemical composition of leaves and petioles/stems were very different. The digestibility of the leaf fraction was often much lower than that of the petiole/stem fraction, but its nitrogen content was higher. Reasons for this, and their implications, are discussed. Protein degradability in the rumen was lower than expected (<70%); this was attributed to very fast rates of flow of digesta out of the rumen. Herbage minerals generally appeared to be adequate. Implications of the degree of degradation of individual amino acids and minerals in the rumen are discussed. The use of chemical characteristics to predict the digestibility of Persian clover herbage is considered and discussed.

scholarly journals Chemical composition and nutritional quality of short-ripened rennet cheeses produced by traditional methods

2018 ◽  
Vol 74 (10) ◽  
pp. 5971-2018 ◽  
Author(s):  
PRZEMYSŁAW KNYSZ ◽  
MICHAŁ GONDEK ◽  
RENATA PYZ-ŁUKASIK ◽  
MONIKA ZIOMEK ◽  
ŁUKASZ DROZD ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chemical Composition ◽  
G Protein ◽  
Nutritive Value ◽  
Salt Content ◽  
Amino Acid Content ◽  
Traditional Methods ◽  
Dairy Starter Cultures ◽  
Limiting Amino Acids

The aim of the study was to determine the variability in the chemical composition and nutritive value parameters of smoked and unsmoked short-ripened rennet cheeses and unsmoked long-ripened rennet cheeses produced by traditional methods. The raw material for the production of short-ripened cheeses was pasteurized cows’ milk obtained from a dairy, whereas the long-ripened cheeses were manufactured from raw cows’ milk obtained from the producer’s farm. All three varieties of cheese examined were produced with commercial dairy starter cultures. The material for the study was collected in winter, directly at the producers’ retail outlets in southern and eastern Poland. The basic chemical composition was determined according to the Polish Standards, whereas the amino acid profiles of proteins from the cheeses were determined by ion-exchange chromatography. The result analysis revealed significant differences between the different varieties of cheese in terms of their water content, ranging from 32.2% to 42.1%, as well as protein content, which varied from 25.6% to 31.6%. Fat levels ranged between 22.2% and 24%, whereas total ash content amounted to 5.1-5.8%. The significantly highest salt content was found in unsmoked short-ripened cheeses. In all three cheese varieties, the total exogenous amino acid content was comparable, ranging from 46.17 g to 47.36 g/100 g protein, and that of endogenous amino acids varied from 52 g to 53 g/100 g protein. The biological value of proteins was determined by calculating to the chemical score (CS), as described by Mitchell and Block, and the essential amino acid index (EAAI), as described by Oser. A comparison of the results with the standard chicken egg white proteins showed that the limiting amino acids for all varieties of cheese were methionine and cysteine. On the other hand, a comparison with the FAO/WHO-suggested pattern of amino acid requirements (1991) for all age groups over 1 year of age showed that the limiting amino acids were methionine and cysteine in smoked and unsmoked short-ripened cheeses, and treonine in long-ripened cheeses. Considering the chemical indices, such as CS and EAAI, it may be concluded that the traditional rennet cheeses produced in southern and eastern Poland have a favourable amino acid composition of proteins and a high nutritive value..

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and Variety

2011 ◽  
Vol 75 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Anna Kotlarz ◽  
Agnieszka Sujak ◽  
Wacław Strobel ◽  
Wilhelm Grzesiak
Keyword(s):  
Amino Acid ◽  
Chemical Composition ◽  
Crude Oil ◽  
Crude Protein ◽  
Nutritive Value ◽  
Amino Acid Content ◽  
Crude Fibre ◽  
Nutritional Values ◽  
Fiber Fractions ◽  
Pea Seeds

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and VarietyWe tested nutritional usability of pea seeds (Pisum sativumL.) at full maturity. Four white-flowering and 5 colour-flowering new Polish cultivars were compared. We determined proximate composition of ground seeds collected over 4 years period as well as amino acids, minerals (Ca, P, Na, K), tannins and fiber fractions (NDF, ADF, ADL). The seeds contained between 224 and 260 g·kg-1of crude protein which was rich in Lys (6.8±0.8 g) but poor in Met+Cys (2.0±0.2 g·16 g-1N). The amount of tannins (as tannic acid equivalent) in white-flowering cvs was 4.3±0.9 g and in colour-flowering - 7.4±2.2 g. The calculated protein nutritional values were compared against amino acid standards of human and animal nutrition and the whole egg protein. Effects of the cropping year and variety variance in respect of chemical composition of seeds were examined. Crude protein, crude oil, N-free extractives, tannins, K, amino acid content: Leu, Phe+Tyr and Ala content were influenced significantly by the cultivar, while the cropping year had a significant influence on dry matter, crude ash, crude protein, crude fibre, crude oil, N-free extractives ADF and ADL fiber fractions, content of P, Na and K, most of the amino acid levels and on nutritional values of the protein measured by means of CS and EAAI indices.

Chemical composition and in vitro digestibility of nine plant species from Semnan rangeland for camel in Iran

2007 ◽  
Vol 2007 ◽  
pp. 233-233 ◽  
Author(s):  
Armin Towhidi ◽  
Farnoush Rostami ◽  
Reza Masoumi
Keyword(s):  
Chemical Composition ◽  
Plant Species ◽  
Nutritive Value ◽  
Poor Quality ◽  
In Vitro Digestibility ◽  
Gross Energy ◽  
Arid Rangelands

In Iran, Javan (2001) has reported the digestibility some arid rangelands plants by bovine rumen liquor. The determination of in vivo digestibility of wheat straw implies that camel apparently digested poor quality roughages more than cattle and sheep (Cianci et al., 2004). Therefore, It is required to measure the in vitro digestibility of herbages by camel rumen liquor. In province of yazd, nutritive value of 11 different plant species for camel were determined (Towhidi, 2007). The objectives of the current study were to determine 1) the chemical composition, gross energy of the most consuming plant species from rangeland of Semnan province including Seidlitzia rosmarinu, Tamarix tetragyna, Tamarix strica, Halostachys spp, Saudea fruticosa., Alhagi camelorum, Haloxylon ammondendron., Salsola arbescola, Hammada salicornica and, 2) in vitro digestibility of the plants by camel rumen liquor.

Seed yield and nutritive value of dry, mature subterranean clover (Trifolium subterraneum L.)

2001 ◽  
Vol 41 (2) ◽  
pp. 169 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune
Keyword(s):  
Seed Yield ◽  
Dry Matter ◽  
Mineral Content ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
Zinc Content ◽  
Trifolium Subterraneum ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Wide Range

The nutritive value of 26 cultivars of dry, mature subterranean clover was evaluated at Shenton Park, Perth, Western Australia. The cultivars were divided into 3 maturity groups according to flowering time and each cultivar was sown in blocks comprising 4 replicates. The plots were grazed by sheep at 2-week intervals during the growing season. Dry mature plant material and soil were sampled in summer to examine the effect of grazing and cultivar on seed yield and nutritive value of feed residues. Cultivars heavily grazed in spring had a low herbage mass. There was no difference in seed yield and seed weight between heavily and lightly grazed cultivars. Dry matter digestibility and mineral content of dry residues was inconsistent for the 2 grazing treatments. The dry matter digestibility of dry, mature subterranean clover ranged from 40 to 56%, with a wide range of crude fibre, nitrogen and mineral content for the 26 cultivars. While most minerals in the dry residues were above the requirement for sheep, 7 cultivars had a zinc content less than the maintenance requirement for sheep. There was an imbalance for all cultivars in calcium: phosphorus with a range of 4–10: 1. Concurrent estimates on the yield and composition of seed indicated that seed can be resource of minerals for grazing animals in summer. Most cultivars had a seed yield over 100 g/m2 with that of 9 cultivars being over 130 g/m2. Seed was rich in nitrogen, sodium, phosphorus, potassium, magnesium, zinc and copper, and poor in sodium, calcium and manganese. However, there were no cultivars with an appropriate ratio of calcium and phosphorus. The imbalance in nitrogen and sulfur was a result of high nitrogen content with the ratio ranging from 19: 1 to 29: 1.

Plant factors influencing the nutritive value of some temperate annual pasture species

1973 ◽  
Vol 13 (63) ◽  
pp. 404 ◽  
Author(s):  
JG McIvor ◽  
DF Smith
Keyword(s):  
Nutritive Value ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Growth Cycle ◽  
Matter Content ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Hordeum Leporinum ◽  
Brome Grass

Changes in herbage production, dry matter content, nitrogen content and in vitro digestibility of two sown species-subterranean clover (Trifolium subterraneum) and annual ryegrass (Lolium rigidum) - and five common volunteer species-capeweed (Arctotheca calendula), musky crowfoot (Erodium moschatum), barley grass (Hordeum leporinum), ripgut brome grass (Bromus rigidus) and soft brome grass (Bromus mollis)-were measured throughout their growth cycle. The volunteer species compared favourably with the sown species for these measurements and should be capable of providing nutritious grazing for animals.

Pathogenesis-Related Proteins in Trifolium subterraneum: A General Survey and Subsequent Characterisation of a Protein Inducible by Ethephon and Redlegged Earth Mite Attack

1997 ◽  
Vol 24 (6) ◽  
pp. 819 ◽  
Author(s):  
Kevin Broderick ◽  
Christopher Pittock ◽  
Tony Arioli ◽  
Ernest H. Creaser ◽  
Jeremy J. Weinman ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Chitinase Activity ◽  
Pr Proteins ◽  
Gene Encoding ◽  
Pathogenesis Related ◽  
Redlegged Earth Mite ◽  
Ethephon Treatment

One of the chief predators of subterranean clover (Trifolium subterraneum) pastures is redlegged earth mite (Halotydeus destructor; RLEM). Subterranean clover pathogenesis-related (PR) proteins induced by RLEM attack and ethephon treatment were surveyed, and PR proteins with peroxidase, β-1,3-glucanase and chitinase activities were detected. A protein co-migrating with a chitinase activity, induced by RLEM predation and treatment with ethephon, was isolated. It was purified and the N-terminal amino acid sequence determined. Using a degenerate oligonucleotide primer designed from this sequence, a corresponding cDNA fragment was amplified by reverse transcriptase-PCR, then cloned, and used as a probe to screen a subterranean clover cv. Karridale genomic library. The cDNA and a 97% homologous genomic clone were sequenced and the deduced amino acid sequence revealed an open reading frame of 157 amino acids capable of encoding a peptide of 16 478 Da. Significant homology (80%) was found between this protein and an abscisic acid (ABA)-responsive protein from Pisum sativum of unknown function which is an intracellular pathogenesis-related (IPR) protein. The gene encoding this protein also has homology to pea ‘disease response resistance genes’ and to proteins from other plant species in the PR-10 family. The induced protein was designated TsPR-10a due to its homology to other PR-10 proteins. Genomic Southern analysis indicates that the gene encoding this protein, designated Ypr10a, is a member of a multigene family with at least three members. Northern blot analysis indicates that the subterranean clover Ypr10a mRNA, or homologous transcript, level is strongly induced by ethephon treatment in both root and aerial tissues of 3 week old plants. The rapid induction kinetics of Ypr10a mRNA under ethephon treatment, its correlation with a putative chitinase activity, and homology to other PR-protein genes, suggests a pathogenesis-related role for TsPR-10a protein in subterranean clover.

Effect of variety and maturity stage on chemical composition, carbohydrate and protein subfractions, in vitro rumen degradability and energy values of timothy and alfalfa

2003 ◽  
Vol 83 (2) ◽  
pp. 279-290 ◽  
Author(s):  
P. Yu ◽  
D. A. Christensen ◽  
J. J. McKinnon ◽  
J. D. Markert
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Energy Content ◽  
Climatic Conditions ◽  
Phleum Pratense ◽  
Maturity Stage ◽  
Insoluble Protein ◽  
Timothy 2 ◽  
Acid Detergent Fibre

The objective of this study was to investigate the effects of forage variety and stage of maturity on chemical composition, protein and carbohydrate subfractions, in vitro rumen degradability and energy content under the climatic conditions of western Canada. Two varieties of alfalfa (Medicago sativa L. cvs. Pioneer and Beaver) and timothy (Phleum pratense L. cvs. Climax and Joliette), grown at three locations in Saskatchewan (Canada), were cut at three stages [1 = 1 wk before commercial cut (early bud for alfalfa; joint for timothy); 2 = at commercial cut (late bud for alfalfa; pre-bloom head for timothy); and 3 = one week after commercial cut (early bloom for alfalfa; full head for timothy)]. The results showed that: (1) the varieties had minimal effects on nutritional value; however, (2) stage of cutting had a large impact on chemical composition, protein and carbohydrate fractions, in vitro rumen degradability and energy value. As plant maturity advanced from stage 1 to 3, the following changes were noticed: (a) CP was decreased [alfalfa: 20.2 to 17.6; timothy: 11.1 to 7.9% dry matter (DM)]; fibre was affected very little in alfalfa but increased in timothy [acid detergent fibre (ADF): 38.0 to 40.7; neutral detergent fibre (NDF): 68.7 to 72.8% DM]; lignin and neutral detergent insoluble protein (NDIP) were not affected in either forage; acid detergent insoluble protein (ADIP) was increased in alfalfa (3.5 to 17.1% CP), but no effect was seen in timothy; (b) the rapidly degradable protein fraction (PA) was reduced in alfalfa (51.2 to 34.8% CP) but increased in timothy (9.2 to 27.5% CP); the rapidly degradable fraction (PB1) increased in alfalfa (0.0 to 25.2% CP), but decreased in timothy (31.6 to 16.7%); the intermediate degradable fraction (PB2) decreased in both forages (alfalfa: 14.1 to 5.2; timothy: 37.8 to 29.4% CP); the slowly degradable fraction (PB3) declined in alfalfa (33.1 to 17.7% CP), but was not affected in timothy (averaging 21.3% CP); the unavailable fraction (PC) associated with the cell wall increased in alfalfa (3.5 to 17.1% CP), but did not change in timothy (averaging 5.2% CP); (c) in vitro rumen degradabilities of DM (IVDMD) and NDF (IVNDFD) increased at stage 2 and then declined at stage 3. The highest IVDMD and IVNDFD after a 48-h incubation were at cutting stage 2 (50.5 and 37.3%; 42.8 and 37.3% for alfalfa and timothy, respectively); (d) the energy content behaved in a quadratic fashion; it increased initially and then decreased with the highest energy contents at stage 2 for both forages (TDN1x, DE1x, DEp, MEp, NELp: 63.8% DM, 2.92, 2.68, 2.26, 1.40 Mcal kg-1 DM for alfalfa; 45.3% DM, 2.03, 1.86, 1.43, 0.82 Mcal kg-1 DM for timothy, respectively). In general, the best cutting was found at stage 2, which was late-bud for alfalfa varieties and pre-bloom head for timothy. Key words: Alfalfa, timothy, variety, maturity, nutritive value

Degradability of crude protein from clover herbages used in irrigated dairy production systems in northern Victoria

2001 ◽  
Vol 52 (3) ◽  
pp. 415 ◽  
Author(s):  
D. C. Cohen
Keyword(s):  
Protein Content ◽  
White Clover ◽  
Crude Protein ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Crude Protein Content ◽  
Persian Clover ◽  
Protein Supply ◽  
In Sacco

An in sacco (nylon bag) technique was used to estimate the degradability of dry matter (DM) and crude protein, and to estimate the effective rumen degradability of protein (ERDP), for 3 irrigated clover herbages. Pasture characteristics (nutritive values and leaf: stem ratios) were also described, and relationships to ERDP established. The nutritive value characteristics and degradabilities of white clover (Trifolium repens L.), Persian clover (Trifolium resupinatum L.), and subterranean clover (Trifolium subterraneum L.) were also compared for various regrowth periods (of 3, 4, 6, and 12 weeks). Nutritive value characteristics of clover herbages varied from 9.9 to 11.9 MJ/kg DM for metabolisable energy, 153 to 304 g/kg DM for crude protein, and 209 to 377 g/kg DM for neutral detergent fibre. The leaf: stem ratio correlated well with the crude protein content of the clovers, with herbages consisting of more leaf generally having superior crude protein content (R2 = 0.64, P < 0.001). The effective rumen degradability of protein for clover herbages ranged from 60 g/kg DM for mature (12 week regrowth) subterranean clover to 195 g/kg DM for vegetative (3 weeks regrowth) Persian clover. For clover herbages with a 3-week regrowth period, after initial cutting to 5 cm, and at ruminal outflow rates of 0.08/h, the effective rumen degradability of protein varied from 136 to 195 g/kg DM. A positive relationship between crude protein and ERDP (R2 = 0.82) suggested that ERDP could be estimated for clover herbages that have been previously assessed for crude protein content, obviating the need for in sacco studies. Calculated degradabilities were lower for all clovers when higher ruminal outflow rates were assumed. Using the metabolisable protein system, metabolisable protein supply and metabolisable protein in excess of animal requirements were calculated. Ruminal losses of nitrogen were also estimated for cows consuming white clover of varying regrowth periods in both early and late lactation. It was concluded that metabolisable protein supply is unlikely to limit production in these examples. At regrowth periods of 3 weeks, metabolisable protein contributions from microbial and dietary sources were similar, and twice that required by the animal. Ruminal losses of nitrogen were substantial and amounted to 66—23% of nitrogen intake. In the experiments reported here, if all of the energy required to excrete excess protein (as urea) could instead be used for the production of milk, cows may have produced 0.5mp;mdash;2.0 kg more milk per day. Such losses could potentially be reduced if the protein content and/or degradabilities of clover herbages were reduced, and/or energy rich supplements were offered.

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