Utilisation of forage rape (Brassica napus) and Persian clover (Trifolium resupinatum) diets by sheep: effects on whole tract digestibility and rumen parameters

2010 ◽  
Vol 50 (1) ◽  
pp. 59 ◽  
Author(s):  
R. Kaur ◽  
S. C. Garcia ◽  
W. J. Fulkerson ◽  
I. Barchia

This study investigated the effect of increasing the proportion of forage rape or Persian clover in the diet on dry matter intake (DMI), whole tract digestibility and rumen function. Two experiments were conducted simultaneously; each in a 3 by 3 Latin square design with rumen-fistulated sheep fed either increasing amounts (10, 25 or 40% DM of the diet) of forage rape (experiment 1) or Persian clover (experiment 2). The remaining diet comprised short-rotation ryegrass, cereal-based pellets and maize silage, formulated to give similar metabolisable energy (MJ/kg DM), neutral detergent fibre and protein values between treatments. Purine derivatives excreted in the urine were used to estimate microbial protein synthesis. The study found similar DMI within forage rape and Persian clover treatments, although total DMI was 16% higher for the latter (29 v. 25 g/kg liveweight.day, respectively). The in vivo DM (mean = 0.75) and neutral detergent fibre (mean = 0.65) digestibility coefficients were similar (P > 0.05) within and between experiments. The mean (±s.e.) rumen pH in sheep fed forage rape-based diets (6.17 ± 0.10) was higher than for Persian clover-based diets (5.70 ± 0.09). The efficiency of microbial N supply expressed as microbial N (g/day) per kg of digestible organic matter fermented in the rumen was also similar (P > 0.05) within and between forage rape and Persian clover treatments (mean = 23.4 g microbial N/kg digestible organic matter fermented). The results indicate that increasing the proportion of high nutritive value forage rape or Persian clover within the diet of sheep did not affect the efficiency of feed utilisation.

1992 ◽  
Vol 55 (2) ◽  
pp. 285-287 ◽  
Author(s):  
A. Karalazos ◽  
D. Dotas ◽  
J. Bikos

Four castrated crossbred (Karagouniki♂ × Friesian♀) rams were used in a 4 × 4 Latin-square design to determine the apparent digestibility of four diets based on alfalfa hay and maize grain and containing varying proportions of whole cottonseed (WCS). The proportions of WCS studied were 0, 175, 355 and 530 g/kg diet dry matter (DM) and the respective apparent digestibility coefficients of DM were 0·744, 0·716, 0·689 and 0·625; of organic matter, 0·772, 0·739, 0·718 and 0·643; and of neutral-detergent fibre (NDF), 0·409, 0·481, 0·522 and 0·472. The gross energy (GE) apparent digestibility coefficients were 0·745, 0·722, 0·704 and 0·641, for the same order of treatments. The effect of the incorporation of WCS instead of maize grain in the diet in significantly reducing DM, OM and GE digestibility was evident. The lowest digestible energy value of WCS was observed in the treatment with the highest level of WCS.


Author(s):  
D I Givens ◽  
Jeannie M Everington

Despite its importance, relatively few measurements have been made of the metabolisable energy (ME) content of fresh herbage. Changes in the nutritive value of spring herbage are commonly described in terms of its digestible organic matter content (DOMD) either measured in vitro or predicted from a fibre fraction. ME is normally assumed to be directly proportional to DOMD.Energetic studies of herbage such as those of Armstrong (1964) and Beever et al (1985) have been restricted to pure species and one location. The purpose of the present experiment was to examine changes in DOMD and energy values with increasing maturity in vivo using sheep in commercial swards over several years.


2010 ◽  
Vol 55 (No. 9) ◽  
pp. 372-381 ◽  
Author(s):  
V. Koukolová ◽  
P. Homolka ◽  
O. Koukol ◽  
F. Jančík

Seven clover (Trifolium pratense L.) samples were collected at three different stages of the same sward (first growth (I), n = 3; first regrowth (II), n = 3; second regrowth (III), n = 1) during the growing season from 10<sup>th</sup> of May to 17<sup>th</sup> of August. Samples were analyzed for chemical composition, gross energy (GE) content, in vivo organic matter digestibility (OMD) and gross energy digestibility (GED) in sheep, and in situ rumen degradability of neutral detergent fibre (NDF). The contents of ash, crude protein (CP), crude fibre (CF), NDF, acid detergent fibre (ADF), acid detergent lignin (ADL) and GE were significantly (P &lt; 0.05) affected by the time of cutting. Average values of 119.2, 197.7, 214.1, 400.7, 296.2, 73.8 g/kg of dry matter (DM) and 18.2 MJ/kg of DM were obtained for ash, CP, CF, NDF, ADF, ADL and GE, respectively. In general, OMD and GED decreased as the cutting time progressed, with average values of 72.4% and 70.2%, respectively. Effective ruminal degradability (ED) of NDF generally decreased (P &lt; 0.05) with the increasing date of cutting at each stage, with the values 66.1% (May 10), 63.6% (May 18), 59.2% (May 25), 64.8% (June 29), 57.4% (July 7), 56.9% (July 13) and 51.6% (August 17). In situ measurements were characterised by an average value of 77.1% for the fraction of NDF potentially degradable in the rumen (b), 0.0703 h&ndash;1 for the rate constant of disappearance of fraction b (c), and 77.7% for digestible NDF (DNDF).


1990 ◽  
Vol 114 (1) ◽  
pp. 49-54 ◽  
Author(s):  
G. J. Cruickshank ◽  
D. P. Poppi ◽  
A. R. Sykes ◽  
A. S. Familton

SUMMARYThe voluntary food intake, in vivo digestibility and site of nutrient digestion was measured in 12 Polled Dorset ram lambs, from 6 to 24 weeks of age, offered clover hay. Lambs were weaned at 6 weeks of age. Six were fitted with a permanent T-shaped cannula in the abomasum and a temporary rumen catheter during the periods of digesta flow measurement.Surgical modification of lambs did not influence intake (g/kg live weight (W) per day), in vivo digestibility or marker retention time in the rumen. However, intact lambs grew significantly faster than cannulated lambs (139 v. 109g/day, P < 0·05) apparently because of an overall higher cumulative intake over the whole experimental period rather than any change in efficiency of use of nutrients or maintenance requirement.Relative to live weight, intake increased rapidly until c. 10 weeks of age and remained relatively constant at c. 37 g DM/kg W per day for the remainder of the experiment. Age had no effect on in vivo digestibility of organic matter (OM) or neutral detergent fibre (NDF) (average 0·69).The proportion of digestible organic matter apparently digested in the rumen (DOMADR) decreased from 0·63 to 0·47 between 8 and 24 weeks of age whilst intake between these measurement periods increased. Nonammonia N (NAN) flow past the abomasum was similar at all ages in relation to digestible organic matter intake (55·1 g NAN/kg DOMI) but was higher at 16 and 24 weeks of age than at 8 and 12 weeks of age, respectively, in relation to organic matter apparently digested in the rumen (OMADR) (116 and 85 g NAN/kg OMADR, respectively, P < 0·05).Fractional outflow rate of digesta markers from the rumen, when estimated from abomasal digesta, was significantly higher than when estimated from faeces (103Ru-p 0·121 v. 0·09; 51Cr-EDTA 0·169 v. 0·111/h; P > 0·05).It was concluded that cannulation did not affect intake and digestion characteristics in these young lambs. The changes in the site of nutrient digestion as the animals aged may have been due to agerelated changes in digestive function but appeared to be associated with the increase in intake that occurred between measurement periods of the cannulated lambs.


2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.


2002 ◽  
Vol 82 (4) ◽  
pp. 621-626 ◽  
Author(s):  
H. A. Lardner ◽  
S. B. M. Wright ◽  
R. D. H. Cohen ◽  
P. Curry ◽  
L. MacFarlane

Rejuvenation of forage stands is probably the most economic and practical method to improve production and quality of forage stands. Animal data are ultimately needed to validate the viability of the rejuvenation technique. This study determined animal responses to changes in forage nutrient composition following three methods of rejuvenation. Smooth bromegrass (Bromus inermis L.) and alfalfa (Medicago sativa L.) hay was harvested from rejuvenated plots on Black Chernozemic and Gray Luvisolic soils in Saskatchewan and fed to 24 ram lambs. The hay was harvested at two stages of maturity from plots that underwent the following rejuvenation techniques, selected to provide a range of responses: deep-banded liquid plus broadcast granular fertilizer (providing 200 kg N ha-1, 90 kg P2O5 ha-1, 46 kg K2O ha-1, 24 kg S ha-1), spring burn, and control. As maturity advanced, digestible organic matter intake (DOMI) (g d-1 kg-0.75) decreased (P < 0.05) for all diets harvested from burn and fertilizer plots. DMI (g d-1) and DOMI were always greater (P < 0.05) for hay (both early or late harvest) from the rejuvenated plots than from control plots. Grass-legume hay harvested early from spring burn treatment from the Gray Luvi solic soil site had a greater (P < 0.05) voluntary intake, (85.4 g d-1 kg-0.75) as compared with forage from control plots (76.2 g d-1 kg-0.75). Apparent digestibilities of DM and organic matter were greater (P < 0.01) for early harvested forage from control plots at the Gray Luvisolic soil site than early harvested hay from the fertilized plots. Metabolizable energy content was higher (P < 0.05) for early harvested hay from burn and fertilized plots at the Black soil site than hay from control plots. These results suggest that existing forage species, if harvested early, can be responsive to rejuvenation, resulting in improved animal performance. Key words: Rejuvenation, fertilizer, burn, lamb, grass-legume hay


1996 ◽  
Vol 1996 ◽  
pp. 41-41
Author(s):  
A.T. Adesogan ◽  
E. Owen ◽  
D.I. Givens

Estimates of the metabolisable energy (ME) content of whole crop wheat (WCW) derived using measured energy losses as methane (ELMm) are lacking due to the cost of measuring ELMm. Published ME values of WCW are largely calculated using predicted energy losses as methane (ELMp, Blaxter and Clapperton, 1965) or digestible organic matter content (DOMD) in vivo. However, there appears to be no published information about the accuracy with which DOMD in vivo or ELMp predicts the ME content of WCW. Therefore, this study assessed the validity of such ME predictions by comparing them with ME contents calculated using ELMm.


2003 ◽  
Vol 76 (2) ◽  
pp. 319-326
Author(s):  
J. van E. Nolte ◽  
A.V. Ferreirat ◽  
H.H. Köster

AbstractA 5 ✕ 5 Latin square was conducted with ruminally and duodenally cannulated Dohne Merino wethers consuming wheat straw to determine the effect of different rumen degradable protein (RDP) levels on forage intake, fermentation characteristics, nutrient flow and digestion. The wethers had ad libitum access to water and wheat straw (32 g crude protein (CP) per kg dry matter (DM); 742 g neutral-detergent fibre (NDF) per kg DM) that was offered twice daily, immediately after intraruminal infusion of the supplements at 07:00 and 19:00 h. The supplemental RDP (calcium caseinate; 900 g CP per kg DM) levels were: 0, 40, 80, 120 and 160 g/day. Each period consisted of 14 days of adaptation and 6 days of sampling. Forage and total organic matter (OM) intakes increased in a linear manner (P < 0•01) with increasing supplemental RDP levels. Digestible organic matter intake (DOMI) displayed a quadratic increase with elevated amounts of RDP (P < 0•01). The effects of treatments on rumen and total tract digestion, as well as fluid dilution rate were minimal. Microbial nitrogen (MN) flow to the duodenum and microbial efficiency increased quadratically (P < 0•04) with increased RDP supplementation. Rumen ammonia nitrogen (NH3-N) concentrations increased linearly (P < 0•01) and total volatile fatty acids (VFA) tended to increase linearly (P = 0•07). In conclusion, RDP supplementation to Dohne Merino wethers consuming wheat straw generally enhanced rumen fermentation and forage intake. A total RDP intake (sources: calcium caseinate and wheat straw) of 3•30 g/kg M0•75 or 0•12 of DOM maximized DOMI.


2018 ◽  
Vol 39 (4) ◽  
pp. 1717
Author(s):  
Antonio Carlos Homem Junior ◽  
Jane Maria Bertocco Ezequiel ◽  
Leonardo Fernandes Cremasco ◽  
Marco Túlio Costa Almeida ◽  
Josimari Regina Paschoaloto ◽  
...  

The objective of this study was to evaluate in vitro nutrient disappearance and in vivo nutrient digestibility of cattle diets containing 70 or 30% roughage, with and without glycerin supplementation. Four Nellore cattle were used in the study based on a 4 × 4 Latin Square design in a 2 × 2 factorial arrangement. Inclusion of glycerin and roughage proportion did not affect the nutrient disappearance of each ingredient (P > 0.05). Regardless of the inclusion of glycerin, a reduction in the proportion of roughage led to an increase (P < 0.05) in DMDis (64 vs. 72%), NDFDis (41 vs. 54%), and ADFDis (31 vs. 44%) of the total rations. Inclusion of glycerin resulted in an increase (P < 0.05) in DMD (70 vs. 62%) and a reduction (P < 0.05) in CPD (32 vs. 38%) and STD (82 vs. 74%). The diet containing 30% roughage led to higher (P < 0.05) DMD (67 vs. 65%) and CPD (35 vs. 33%) in comparison with the 70% roughage diet, but did not differ (P > 0.05) in STD (78%). Inclusion of glycerin at 20% of the total DM increased the utilisation of dietary DM without affecting the fibrous fraction of the diet. Glycerin supplementation in diets containing 70% roughage improves neutral detergent fibre digestibility.


2012 ◽  
Vol 109 (10) ◽  
pp. 1765-1778 ◽  
Author(s):  
Abdelhafid Keli ◽  
Genaro Olmos ◽  
Antonio de Vega ◽  
José A. Guada

An experiment was carried out to examine the effect of increasing the proportion of Wimmera ryegrass hay in a lucerne hay-based diet on net transfer of nutrients to the intestine, and on the disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep. Following a latin square design, four adult ewes were fed 1:0, 0·33:0·67, 0·67:0·33 and 0:1 proportions of legume and grass. Increasing the proportion of ryegrass in the diet linearly decreased the intake of DM (P= 0·017), organic matter (P= 0·021) and N (P= 0·001). However, neutral-detergent fibre intake was not affected (P= 0·148), nor was its digestibility coefficient (P>0·10). Diet had no effect on duodenal flows of nutrients (P>0·10), although the proportion of N intake (NI) recovered at the duodenum as non-NH3N (NAN) increased linearly withLolium rigidumin the diet (P= 0·002). Full recovery of NI as NAN was achieved at NH3concentrations in the rumen below 110 g/l. Microbial N contribution to NAN varied in a quadratic manner (P< 0·05) with the proportion of grass in the diet, although efficiency of microbial synthesis was not affected (P>0·10). Duodenal recovery of consumedn-alkanes was not affected by diet and was complete for those present in higher concentrations in the forages. Isolated rumen bacteria contained significant amounts ofn-alkanes, contributing to the duodenal flow of these hydrocarbons in variable proportions depending on the diet consumed.


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