Plant-Dependent Soil Bacterial Responses Following Amendment With a Multispecies Microbial Biostimulant Compared to Rock Mineral and Chemical Fertilizers

Biostimulants are gaining momentum as potential soil amendments to increase plant health and productivity. Plant growth responses to some biostimulants and poorly soluble fertilizers could increase soil microbial diversity and provide greater plant access to less soluble nutrients. We assessed an agricultural soil amended with a multispecies microbial biostimulant in comparison with two fertilizers that differed in elemental solubilities to identify effects on soil bacterial communities associated with two annual pasture species (subterranean clover and Wimmera ryegrass). The treatments applied were: a multispecies microbial biostimulant, a poorly soluble rock mineral fertilizer at a rate of 5.6 kg P ha–1, a chemical fertilizer at a rate of 5.6 kg P ha–1, and a negative control with no fertilizer or microbial biostimulant. The two annual pasture species were grown separately for 10 weeks in a glasshouse with soil maintained at 70% of field capacity. Soil bacteria were studied using 16S rRNA with 27F and 519R bacterial primers on the Mi-seq platform. The microbial biostimulant had no effect on growth of either of the pasture species. However, it did influence soil biodiversity in a way that was dependent on the plant species. While application of the fertilizers increased plant growth, they were both associated with the lowest diversity of the soil bacterial community based on Fisher and Inverse Simpson indices. Additionally, these responses were plant-dependent; soil bacterial richness was highly correlated with soil pH for subterranean clover but not for Wimmera ryegrass. Soil bacterial richness was lowest following application of each fertilizer when subterranean clover was grown. In contrast, for Wimmera ryegrass, soil bacterial richness was lowest for the control and rock mineral fertilizer. Beta diversity at the bacterial OTU level of resolution by permanova demonstrated a significant impact of soil amendments, plant species and an interaction between plant type and soil amendments. This experiment highlights the complexity of how soil amendments, including microbial biostimulants, may influence soil bacterial communities associated with different plant species, and shows that caution is required when linking soil biodiversity to plant growth. In this case, the microbial biostimulant influenced soil biodiversity without influencing plant growth.

Use of barley crops, and combinations of lucerne and Wimmera grass, as pasture for grazing sheep in the semiarid zones of Spain

Taking into account the relationship between rainfall and temperature, 64% of the Spanish territory can be classified as arid (25%) or semiarid (39%). In these areas, vegetal surface includes permanent pastures and drought grasslands (34.0%), fallows (13.4%), winter cereals for grain production (21.2%), forage crops (mainly lucerne, and winter cereals harvested as green forage; 2.6%) and woodland hills. Ruminants in these zones account for 15.4 million head, of which nearly 80% are sheep. In the present paper, the possibilities of integrating sheep in cropping systems are discussed, and an alternative based on the use of permanent sowed prairies (lucerne), self-sowing annuals (Wimmera ryegrass) and winter cereals (barley) will be analysed.

Net transfer of nutrients to the duodenum and disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep fed grass/legume combinations

An experiment was carried out to examine the effect of increasing the proportion of Wimmera ryegrass hay in a lucerne hay-based diet on net transfer of nutrients to the intestine, and on the disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep. Following a latin square design, four adult ewes were fed 1:0, 0·33:0·67, 0·67:0·33 and 0:1 proportions of legume and grass. Increasing the proportion of ryegrass in the diet linearly decreased the intake of DM (P= 0·017), organic matter (P= 0·021) and N (P= 0·001). However, neutral-detergent fibre intake was not affected (P= 0·148), nor was its digestibility coefficient (P>0·10). Diet had no effect on duodenal flows of nutrients (P>0·10), although the proportion of N intake (NI) recovered at the duodenum as non-NH3N (NAN) increased linearly withLolium rigidumin the diet (P= 0·002). Full recovery of NI as NAN was achieved at NH3concentrations in the rumen below 110 g/l. Microbial N contribution to NAN varied in a quadratic manner (P< 0·05) with the proportion of grass in the diet, although efficiency of microbial synthesis was not affected (P>0·10). Duodenal recovery of consumedn-alkanes was not affected by diet and was complete for those present in higher concentrations in the forages. Isolated rumen bacteria contained significant amounts ofn-alkanes, contributing to the duodenal flow of these hydrocarbons in variable proportions depending on the diet consumed.

Initial activity of atrazine and chlorsulfuron residues is greater in the grey clays than in the black earths of southern Queensland

Commercial experience is that soil type appears to be a major factor influencing initial weed control from soil applications of atrazine and chlorsulfuron. We tested this by collecting 26 soils from the major cropping areas in southern Queensland. Initial activity was determined by measuring the dose required for 50% inhibition (ID50) of seedling growth in Wimmera ryegrass in controlled environment cabinets. The mean ID50 for atrazine and chlorsulfuron in black earths was 3 and 4 times greater, respectively, than in grey clays. Lower activity in the black earths was probably due to higher clay and cation contents and, consequently, greater adsorption of the herbicides. These results suggest that application rates of atrazine and chlorsulfuron can be adjusted for differences in initial activity between soil types.

Selection by grazing sheep of pasture plants at low herbage availability and responses of the plants to grazing

Merino sheep grazing annual pasture at the beginning of the growing season when the amount of herbage on offer was small, preferred to graze Wimmera ryegrass Lolium rigidum or subterranean clover Trifolium subterraneum rather than capeweed Arctotheca calendula, and Erodium botrys was avoided completely. Behaviour observations showed that capeweed plants were usually avoided. When the plants were grasped they were sometimes pulled up by the roots and then dropped so that the number of capeweed plants in the pasture declined. Supplementation with oats reduced grass intake. Harvesting behaviour changed with pasture conditions: as grass height declined in the pasture, the rates of biting, stepping and head swinging increased. Pasture measurements showed that, whilst capeweed plants continued to increase in height during grazing, as did ungrazed controls, ryegrass and clover plants decreased or remained short. Herbage dry matter increased in all species, owing especially to basal growth. The proportion of shoots and petioles which were erect increased in ungrazed plants, but the proportion which were prostrate was much greater in grazed plants. Individual plants adapted their growth form in a way which counteracted the depredations of grazers. The ecological implications of these findings are important. Firstly, the sheep were not foraging optimally in terms of maximising rate of intake, since two abundant species were largely ignored even though food availability was low. Secondly, because of their selectivity the sheep were giving the capeweed and Erodium a competitive advantage which, in these pastures, will persist through the growing season.

Competition between Wimmera ryegrass and narrow-leafed lupins

The ways in which Wimmera ryegrass (Lolium rigidum) affects the growth and yield of narrow-leafed lupins (Lupinus angustifolius) were studied in two experiments at Baker's Hill, W.A. (average annual rainfall 650 mm). In the first experiment, Uniharvest lupins and Wimmera ryegrass were grown alone and together starting at two dates. The three rates of ryegrass produced similar biomasses (total dry matter) at maturity (about 8000 kg/ha) but lupin grain yield decreased significantly with ryegrass sowing rate. One effect of ryegrass at the highest sowing rate was to alter the distribution of dry matter in the lupin canopy so that, at the end of the growing season, there was a lower percentage of leaf in the lower part of the canopy and this was associated with less light being received. Water stress in lupins at the end of the growing season was higher at the later planting with ryegrass. A multiple regression analysis showed that 74% of the variation in lupin grain yield between plots was due to variation in lupin height and biomass, and ryegrass tiller numbers in October. Lupins had little effect on ryegrass yield; although there were fewer tillers in the presence of lupins, weights of tillers were higher. In the second experiment, the effects of 38 ryegrass plants/m2 on growth of both Uniharvest and Unicrop lupins grown at a density of 40 plants/m2 were studied. Lupin biomass was unaffected until October but was then reduced significantly by the ryegrass, which yielded 4000 kg/ha dry matter. Grain yield was reduced because fewer pods were produced on lateral branches in both varieties, and on main stems in Uniharvest. The experiments showed that in good growing seasons, in high rainfall areas, lupins will yield grain even when grown with very high densities of ryegrass.

Sulfur nutrition of temperate pasture species. II. A comparison of subterranean clover cultivars, medics and grasses

The external and internal requirements for sulfur of five pasture legumes and three grasses of temperate origin were examined in a pot experiment in which plants were grown with seven levels of sulfur supply for 58 days. Nitrogen supply was non-limiting. There was no distinct difference between legumes and grasses in their external requirement for sulfur (i.e. the amount of sulfur required for 90% of maximum yield) or in their sensitivity to sulfur deficiency (i.e. yield at the lowest sulfur supply expressed as a percentage of maximum yield). The requirement for sulfur (mg/pot) fell in the following order: Hunter River lucerne (24) > Jemalong barrel medic (19) > brome grass and Wimmera ryegrass (18) > barley grass (15) > Clare subterranean clover (13) > Trikkala subterranean clover (11) > Seaton Park subterranean clover (10). The high external requirement for sulfur of Hunter River lucerne appears to be due to its poor ability to distribute sulfur from root to shoot and to its high internal requirement for sulfur. However, for Jemalong barrel medic, the high external requirement was solely due to its high internal requirement for sulfur. The higher external requirement for sulfur of the grasses compared with the subterranean clover cultivars appears to be due to the poorer ability of the grasses to obtain sulfur from the soil used in this experiment, rather than to differences in the transfer of sulfur from root to shoot, or in their internal requirement for sulfur.

Feeding value of subterranean clover, lucerne, phalaris and Wimmera ryegrass for lambs

The feeding values of subterranean clover, lucerne, phalaris and Wimmera ryegrass were measured in three experiments using weaned lambs. With dried herbage, the voluntary intake of each species increased linearly with organic matter digestibility over the range tested (57-83%). Regressions for the two grasses did not differ from each other, nor did those for the two legumes; a common regression for the legumes had the same slope as that for the grasses but dry matter intake was about 190 g/d greater on legume diets. Digestibility accounted for 50 and 68% of variation in intake of legumes and grasses, respectively, but when cellulose concentration in the diet was used as the predictor, a single regression satisfied all the data and accounted for 76% of variation in intake. Energy retention by weaned lambs offered diets of dried subterranean clover or lucerne was 53% greater than that by lambs receiving the same intake of metabolizable energy from phalaris. When the intake of the two legume diets was unrestricted, this difference increased to 128%. Despite the 30% higher voluntary intake of the legume diets, the weight of digesta in the reticula-rumen at the end of a meal was the same as it was for the phalaris diet. When all four species were compared in the field, with herbage amply available, lambs grazing the two legumes retained 57% more energy than those on the grasses during the main spring period when the species were of comparable digestibility. As there was little or no difference, under the conditions of this experiment, in the intake of food from the four swards, it is concluded that the difference in feeding value resulted from more efficient use of metabolizable energy. Overall, subterranean clover had as high a feeding value for lambs as lucerne, and phalaris was not inferior to Wimmera ryegrass.