scholarly journals Net transfer of nutrients to the duodenum and disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep fed grass/legume combinations

2012 ◽  
Vol 109 (10) ◽  
pp. 1765-1778 ◽  
Author(s):  
Abdelhafid Keli ◽  
Genaro Olmos ◽  
Antonio de Vega ◽  
José A. Guada
Keyword(s):  
Organic Matter ◽  
Latin Square ◽  
Rumen Bacteria ◽  
Lolium Rigidum ◽  
Microbial Synthesis ◽  
Fibre Intake ◽  
Wimmera Ryegrass ◽  
Ryegrass Hay ◽  
Microbial N ◽  
Net Transfer

An experiment was carried out to examine the effect of increasing the proportion of Wimmera ryegrass hay in a lucerne hay-based diet on net transfer of nutrients to the intestine, and on the disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep. Following a latin square design, four adult ewes were fed 1:0, 0·33:0·67, 0·67:0·33 and 0:1 proportions of legume and grass. Increasing the proportion of ryegrass in the diet linearly decreased the intake of DM (P= 0·017), organic matter (P= 0·021) and N (P= 0·001). However, neutral-detergent fibre intake was not affected (P= 0·148), nor was its digestibility coefficient (P>0·10). Diet had no effect on duodenal flows of nutrients (P>0·10), although the proportion of N intake (NI) recovered at the duodenum as non-NH3N (NAN) increased linearly withLolium rigidumin the diet (P= 0·002). Full recovery of NI as NAN was achieved at NH3concentrations in the rumen below 110 g/l. Microbial N contribution to NAN varied in a quadratic manner (P< 0·05) with the proportion of grass in the diet, although efficiency of microbial synthesis was not affected (P>0·10). Duodenal recovery of consumedn-alkanes was not affected by diet and was complete for those present in higher concentrations in the forages. Isolated rumen bacteria contained significant amounts ofn-alkanes, contributing to the duodenal flow of these hydrocarbons in variable proportions depending on the diet consumed.

scholarly journals The simultaneous estimation of the amounts of protozoal, bacterial and dietary nitrogen entering the duodenum of steers

1984 ◽  
Vol 51 (1) ◽  
pp. 111-132 ◽  
Author(s):  
J. E. Cockburn ◽  
A. P. Williams
Keyword(s):  
Latin Square ◽  
Diaminopimelic Acid ◽  
Simultaneous Estimation ◽  
Rumen Bacteria ◽  
Bacterial Protein ◽  
Flow Estimation ◽  
Dietary Nitrogen ◽  
Amino Acid Profiling ◽  
Dietary Casein ◽  
Microbial N

1. Four steers were given straw and tapioca diets, twice daily, in a 4 x 4 Latin-square design. These diets, containing 4.2 g nitrogen/kg dry matter (DM), were further supplemented with either urea, decorticated groundnut meal (DCGM), untreated (UT) casein or formaldehyde-treated (FT) casein to give a total of 19.7 g N/kg DM and 10.5 MJ/kg DM daily.2. Concurrent samples of rumen bacteria and protozoa and abomasal digesta were collected for each period of the experiment and the concentrations of 2-aminoethyl phosphonic acid (AEPA), diaminopimelic acid (DAPA), total nitrogen (TN), total phosphorus (TP), amino acids and hexosamines were determined in the dried preparations. The nature of the dietary supplements had little effect on the concentrations of most of these constituents or on the total protozoal numbers.3. Abomasal digesta samples marked with polyethylene glycol (PEG) and chromic oxide for flow estimation were collected over 24 h, and the proportions of protozoal-N, bacterial-N and microbial-N estimated simultaneously using the markers AEPA, DAPA and RNA respectively. These digesta-N components were also estimated using an amino acid profiling (AAP) method which gave, in addition, estimates of the dietary and endogenous components. For the diets containing casein, the proportion of dietary casein was estimated directly using casein-P as a marker.4. Estimates of the respective mean proportions of microbial-N in abomasal digesta non-ammonia-N (NAN) for the diets containing urea, DCGM, UT casein or FT casein were: AEPA 0.56, 0.32, 0.27 and 0.16; DAPA 0.88, 0.70, 0.81 and 0.57; RNA 0.98, 0.85, 0.92 and 0.53.5. Giving FT casein significantly (P< 0.001) increased the flow of casein-N at the abomasum and a significantly (P< 0.001) greater proportion of casein-N was found in abomasal NAN (0.51v.0.09) where FT rather than UT casein was given.6. The AAP method gave results for the proportions of microbial- and dietary-N (where casein was given) which were, in general, slightly lower than those obtained using RNA and casein-P as markers. Agreement with estimates of bacterial protein (from DAPA) and of protozoal protein (from AEPA) was less satisfactory.7. Comparisons of the various estimates of the proportions of microbial-N in abomasal digesta suggested that the results obtained for protozoal-N by AEPA were overestimates. AEPA was found in mixed rumen bacteria which may have accounted in part for these overestimates. However, AEPA was not detected in any of the dietary ingredients.

Measurement of protozoa, using phosphatidyl choline, and of bacteria, using nucleic acids, in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativaL.) diets

1984 ◽  
Vol 102 (1) ◽  
pp. 33-44 ◽  
Author(s):  
A. John ◽  
M. J. Ulyatt
Keyword(s):  
Organic Matter ◽  
Dietary Intake ◽  
Feed Intake ◽  
Phosphatidyl Choline ◽  
Close Agreement ◽  
Rumen Bacteria ◽  
Once Daily ◽  
Rumen Protozoa ◽  
N Yield ◽  
Microbial N

SummaryThe use of phosphatidyl choline (PC) as a marker of protozoa in duodenal digesta was examined in sheep fed chaffed lucerne hay (Medicago sativaL.; 330–950 g organic matter/day) either once daily or hourly.PC was present in rumen protozoa (N:PC-P = 185±6·4) but was not detected in rumen bacteria. Dietary intake of PC was less than 5% of the daily passage of PC from the stomach (7·49 mg PC-P/day). A comparison of the SA of PC-P in protozoa and in duodenal digesta of sheep given a continuous intraruminal infusion of32P-orthophosphate showed that the proportion of dietary PC-P in duodenal digesta was negligible.The limitations and validity of PC as a marker of protozoa in duodenal digesta are discussed. The daily passage of protozoal N from the stomach (1·38 ± 0·125 g/day) estimated with PC was not affected by frequency of feeding but protozoal N yield (4·96 ± 0·509 g/kg organic matter apparently digested) declined with increasing feed intake.The SA of DNA-32P, RNA-32P and readily extractable phospholipid-32P (REPL-32P) in rumen protozoa, bacteria and duodenal digesta were similar, showing that the DNA, RNA and REPL-P in duodenal digesta were of microbial origin. A method is described for the estimation of bacterial REPL-P and bacterial N in duodenal digesta using DNA, RNA or DAPA as markers.The calculated total microbial REPL-P (bacterial plus protozoal) in digesta using DNA or RNA as markers was in close agreement with values obtained by direct measurement. It was estimated that protozoa contributed 22 and 14% of microbial REPL-P and microbial N, respectively. Bacterial REPL-P and bacterial N values estimated with DAPA as the marker were higher than values obtained with DNA or RNA. These results are discussed with regard to the use of these markers.

Utilisation of forage rape (Brassica napus) and Persian clover (Trifolium resupinatum) diets by sheep: effects on whole tract digestibility and rumen parameters

2010 ◽  
Vol 50 (1) ◽  
pp. 59 ◽  
Author(s):  
R. Kaur ◽  
S. C. Garcia ◽  
W. J. Fulkerson ◽  
I. Barchia
Keyword(s):  
Organic Matter ◽  
Nutritive Value ◽  
Latin Square ◽  
Neutral Detergent Fibre ◽  
Short Rotation ◽  
Digestible Organic Matter ◽  
Persian Clover ◽  
Forage Rape ◽  
Microbial N

This study investigated the effect of increasing the proportion of forage rape or Persian clover in the diet on dry matter intake (DMI), whole tract digestibility and rumen function. Two experiments were conducted simultaneously; each in a 3 by 3 Latin square design with rumen-fistulated sheep fed either increasing amounts (10, 25 or 40% DM of the diet) of forage rape (experiment 1) or Persian clover (experiment 2). The remaining diet comprised short-rotation ryegrass, cereal-based pellets and maize silage, formulated to give similar metabolisable energy (MJ/kg DM), neutral detergent fibre and protein values between treatments. Purine derivatives excreted in the urine were used to estimate microbial protein synthesis. The study found similar DMI within forage rape and Persian clover treatments, although total DMI was 16% higher for the latter (29 v. 25 g/kg liveweight.day, respectively). The in vivo DM (mean = 0.75) and neutral detergent fibre (mean = 0.65) digestibility coefficients were similar (P > 0.05) within and between experiments. The mean (±s.e.) rumen pH in sheep fed forage rape-based diets (6.17 ± 0.10) was higher than for Persian clover-based diets (5.70 ± 0.09). The efficiency of microbial N supply expressed as microbial N (g/day) per kg of digestible organic matter fermented in the rumen was also similar (P > 0.05) within and between forage rape and Persian clover treatments (mean = 23.4 g microbial N/kg digestible organic matter fermented). The results indicate that increasing the proportion of high nutritive value forage rape or Persian clover within the diet of sheep did not affect the efficiency of feed utilisation.

scholarly journals What is the digestibility and caloric value of different botanical parts in corn residue to cattle?1

2019 ◽  
Vol 97 (7) ◽  
pp. 3056-3070 ◽  
Author(s):  
Emily A Petzel ◽  
Evan C Titgemeyer ◽  
Alexander J Smart ◽  
Kristin E Hales ◽  
Andrew P Foote ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutrient Availability ◽  
Heat Production ◽  
Latin Square ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Digestible Energy ◽  
Randomized Design ◽  
Corn Residue ◽  
Completely Randomized Design

AbstractTwo experiments were conducted to measure rates of ruminal disappearance, and energy and nutrient availability and N balance among cows fed corn husks, leaves, or stalks. Ruminal disappearance was estimated after incubation of polyester bags containing husks, leaves or stalks in 2 separate ruminally cannulated cows in a completely randomized design. Organic matter (OM) that initially disappeared was greatest for stalks and least for husks and leaves (P < 0.01), but amounts of NDF that initially disappeared was greatest for husks, intermediate for stalks, and least for leaves (P < 0.01). Amounts of DM and OM that slowly disappeared were greatest in husks, intermediate in leaves, and least in stalks (P < 0.01). However, amounts of NDF that slowly disappeared were greatest in leaves, intermediate in husks, and least in stalks (P < 0.01). Rate of DM and OM disappearance was greater for leaves, intermediate for husks and least for stalks, but rate of NDF disappearance was greatest for stalks, intermediate for leaves, and least for husks (P < 0.01). Energy and nutrient availability in husks, leaves, or stalks were measured by feeding ruminally cannulated cows husk-, leaf-, or stalk-based diets in a replicated Latin square. Digestible energy lost as methane was less (P = 0.02) when cows were fed leaves in comparison to husks or stalks, and metabolizable energy (Mcal/kg DM) was greater (P = 0.03) when cows were fed husks and leaves compared with stalks. Heat production (Mcal/d) was not different (P = 0.74) between husks, leaves, or stalks; however, amounts of heat produced as a proportion of digestible energy intake were less (P = 0.05) among cows fed leaves in comparison to stalks or husks. Subsequently, there was a tendency (P = 0.06) for net energy available for maintenance from leaves (1.42 Mcal/kg DM) to be greater than stalks (0.91 Mcal/kg DM), and husks (1.30 Mcal/kg DM) were intermediate. Nitrogen balance was greater when cows were fed leaves, intermediate for husks, and least for stalks (P = 0.01). Total tract digestion of NDF was greater (P < 0.01) for husks and leaves compared with stalks. Husks had greater (P = 0.04) OM digestibility in comparison to stalks, and leaves were intermediate. Apparently, greater production of methane from husks in comparison to leaves limited amounts of energy available for maintenance from husks even though total-tract nutrient digestion was greatest when cows were fed husks or leaves.

The effect of different proportions of hay and fortified rolled barley in the diet on digestible-energy value and urinary-energy excretion by cattle

1972 ◽  
Vol 79 (1) ◽  
pp. 99-103 ◽  
Author(s):  
A. M. Raven
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Progressive Increase ◽  
Linear Increase ◽  
Metabolizable Energy ◽  
Digestible Energy ◽  
Gross Energy ◽  
Concentrate Treatment

SUMMARYA 6 x 6 Latin Square balance experiment was carried out using six Friesian steers, each of which initially weighed about 304 kg. The six treatments studied were an all-hay diet and five other diets containing 20,40,60,80 and 100 % of rolled barley fortified with mineral and vitamin supplements, accompanied by correspondingly reduced proportions of hay. Each diet was fed at an estimated maintenance level of feeding.The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in both digestible and calculated metabolizable energy. The actual increase in digestible energy was from 2·62Mcal/kg dry matter (59·3% of the gross energy) on the all-hay treatment to 3·42 Mcal/kg dry matter (79·5% of the gross energy) on the all-concentrate treatment. Use of the determined digestible energy values for the all-hay and fortified barley diets to calculate the digestible energy of the four mixed diets gave results in reasonably good agreement with the determined values, the maximum difference being 0·12 Mcal/kg dry matter, which represented 3·83 % of the determined value. The losses of energy in the urine expressed as percentages of the gross energy of the diets showed a small but significantly linear decrease (P < 0·01) with increase in proportion of barley in the diet. The molar proportions of steamvolatile acids in samples of rumen fluid taken from two animals on each treatment indicated that increase in the proportion of concentrate was associated with tendencies for increase in acetic acid, decrease in propionic acid and little change in butyric acid. The mean digestibility of the organic matter was 62·6 % on the all-hay treatment and 81·8 % on the all concentrate treatment. The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in digestibility of the organic matter. Although intakes of nitrogen decreased with increase in the proportion of concentrate due to a decrease in the amount of dry matter fed, the weights of nitrogen retained were well maintained and when expressed as percentages of intake showed a significantly linear increase (P < 0·01).

scholarly journals KECERNAAN NDF DAN ADF YANG MENDAPAT SUPLEMENTASI UREA MOLASSES MULTINUTRIENT BLOCK (UMMB) DARI BEBERAPA JENIS LIMBAH PERTANIAN DAN RUMPUT LAPANG PADA SAPI PERANAKAN ONGOLE (PO)

ZOOTEC ◽  
2018 ◽  
Vol 38 (2) ◽  
pp. 320
Author(s):  
Giandi Grahadi Turangan ◽  
B. Tulung ◽  
Y.R.L. Tulung ◽  
M.R. Waani
Keyword(s):  
Body Weight ◽  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Crop Residues ◽  
Latin Square ◽  
Crossbred Cattle ◽  
Corn Straw ◽  
Peanut Straw

NDF AND ADF DIGESTIBILITY WHICH GET THE SUPPLEMENTED UREA MOLASSES MULTINUTRIENT BLOCK (UMMB) OF SOME CROP RESIDUES TYPE AND LOCAL GRASS ON ONGOLE CROSSBRED CATTLE. A research evaluating dry matter (DM) and organic matter (OM) digestibility of crops residues and local grass supplemented urea molasses multinutrient block (UMMB) on Ongole crossbred cattle (PO). Four male PO cattle, 12 – 18 months of age with ± 100 kg of body weight were used in this experiment. Latin Square Design 4 x 4 with 4 cows and 4 treatments was used. The treatments were : R1 : UMMB + local grass, R2: UMMB + Corn straw, R3: UMMB + Rice straw, and R4: UMMB + peanut straw. The results showed that there were significant effect (P<0.01) of treatments on NDF and ADF digestibility. C1 showed that NDF and ADF digestibility of local grass (68.44% and 61.37%) were higher (P<0.05) compared to crop residues (corn straw 65.25% and 58.59%, rice straw 62.63% dan 52.80%, and peanut straw 67.53% and 59.81%) respectively. C2 showed that NDF and ADF digestibility of legume straw was higher than grasses straw; and C3 showed that  NDF and ADF digestibility of rice straw was lower than corn straw. The results have concluded that : 1. NDF and ADF digestibility of local grass were higher compared to crop residues. 2. NDF and ADF digestibility of legume straw (peanut straw) was higher than grasses straw  (corn straw and ricce straw);3. NDF and ADF digestibility of  corn straw was higher than peanut straw.Key words: NDF and ADF digestibility, crop residues, UMMB, ongole crossbred cattle

Effect of leucaena supplementation level, and provision of urea, on utilisation by sheep of Rhodes grass hay

2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rhodes Grass ◽  
Microbial Nitrogen ◽  
Grass Hay ◽  
Digestible Organic Matter ◽  
Ruminal Degradation ◽  
Chloris Gayana ◽  
Dietary Treatments

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.

scholarly journals Intake, digestibility, and ruminal parameters of Nellore bulls supplemented with increasing levels of crude glycerin in Brachiaria Brizantha pastures

2017 ◽  
Vol 18 (4) ◽  
pp. 518-529 ◽  
Author(s):  
Adriano Jorge Possamai ◽  
Joanis Tilemahos Zervoudakis ◽  
Luciana Keiko Hatamoto-Zervoudakis ◽  
Lucien Bissi da Freiria ◽  
Pedro Ivo José Lopes da Rosa Silva ◽  
...  
Keyword(s):  
Organic Matter ◽  
Latin Square ◽  
Serum Glucose ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Brachiaria Brizantha ◽  
Mineral Mixture ◽  
Organic Matter Digestibility ◽  
Four Levels ◽  
Different Levels

SUMMARY We evaluated the effects of increasing levels of crude glycerin in multiple supplements for grazing beef cattle on intake, nutrient digestibility, pH, ruminal ammoniacal nitrogen, and serum glucose. Five Nellore steers (initial BW of 480 kg [SD 15]), grazing a tropical pasture, were used in a 5 × 5 Latin square design. The treatments used were control (no supplementation; only a mineral mixture ad libitum) and four levels of crude glycerin (0, 80, 160, 240 g/kg of supplements). Supplementation type had no impact (P > 0.05) on pH at 0 and 4 h after supplementation. Animals supplemented with different levels of crude glycerin showed higher N-NH3 values (P < 0.05) at 4 h after supplementation. Crude glycerin supplementation did not reduce pasture intake (P > 0.05), increasing dry matter intake (P > 0.05) due to increased organic matter digestibility (P > 0.05). Supplementation of 4 kg/animal with crude glycerin (240 g/kg supplement) did not reduce pasture intake and organic matter digestibility.

scholarly journals Trade-Offs between Sugarcane Straw Removal and Soil Organic Matter in Brazil

2020 ◽  
Vol 12 (22) ◽  
pp. 9363
Author(s):  
Maristela C. Morais ◽  
Marcos Siqueira-Neto ◽  
Henrique P. Guerra ◽  
Lucas S. Satiro ◽  
Amin Soltangheisi ◽  
...  
Keyword(s):  
Organic Matter ◽  
Soil Health ◽  
Removal Rates ◽  
Total N ◽  
No Removal ◽  
Soil C ◽  
Sugarcane Straw ◽  
C Stock ◽  
Microbial C ◽  
Microbial N

Environmental benefits from bioenergy production derived from sugarcane crop residues (straw) can be lost by soil organic matter depletion resulting from excessive straw removal rates from fields. Soil organic carbon stock is the core for sustaining soil health, supporting nutrient cycling, and sequestering carbon dioxide. To find out that how much sugarcane straw can be removed from the field to produce bioenergy without changes in soil C concentrations, we investigated effects of straw removal rates (total, moderate, and no removal of sugarcane straw) on soil carbon and nitrogen fractions in an Oxisol and an Ultisol in southeastern Brazil for two years. Soil C and N fractions were affected by increased rates of straw removal at the second year. In the Oxisol, total straw removal decreased labile and microbial-C by ~30% and soil C stock by 20% compared to no straw removal. No removal decreased microbial-N and total N stock by ~15% and ~20%, respectively. In the Ultisol, no straw removal resulted in increases in C stock by >10% and labile and microbial-C by ~20% related to total straw removal. Total straw removal showed more microbial-N (~10%) and total-N stock (~25%) compared to no straw removal. The moderate straw removal intensity (i.e., 8 to 10 Mg ha−1 of straw) may control the straw-C release to soil by straw decomposition. This study suggests that excessive straw removal rates should be avoided, preventing SOM depletion and consequently, soil health degradation. Moderate straw removal seems to be a promising strategy, but long-term soil C monitoring is fundamental to design more sustainable straw management and bioenergy production systems.

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