scholarly journals Effects of the inclusion of glycerin in diets containing 30 or 70% roughage on feed disappearance and digestibility

2018 ◽  
Vol 39 (4) ◽  
pp. 1717
Author(s):  
Antonio Carlos Homem Junior ◽  
Jane Maria Bertocco Ezequiel ◽  
Leonardo Fernandes Cremasco ◽  
Marco Túlio Costa Almeida ◽  
Josimari Regina Paschoaloto ◽  
...  
Keyword(s):  
Latin Square ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fibre ◽  
Nellore Cattle

The objective of this study was to evaluate in vitro nutrient disappearance and in vivo nutrient digestibility of cattle diets containing 70 or 30% roughage, with and without glycerin supplementation. Four Nellore cattle were used in the study based on a 4 × 4 Latin Square design in a 2 × 2 factorial arrangement. Inclusion of glycerin and roughage proportion did not affect the nutrient disappearance of each ingredient (P > 0.05). Regardless of the inclusion of glycerin, a reduction in the proportion of roughage led to an increase (P < 0.05) in DMDis (64 vs. 72%), NDFDis (41 vs. 54%), and ADFDis (31 vs. 44%) of the total rations. Inclusion of glycerin resulted in an increase (P < 0.05) in DMD (70 vs. 62%) and a reduction (P < 0.05) in CPD (32 vs. 38%) and STD (82 vs. 74%). The diet containing 30% roughage led to higher (P < 0.05) DMD (67 vs. 65%) and CPD (35 vs. 33%) in comparison with the 70% roughage diet, but did not differ (P > 0.05) in STD (78%). Inclusion of glycerin at 20% of the total DM increased the utilisation of dietary DM without affecting the fibrous fraction of the diet. Glycerin supplementation in diets containing 70% roughage improves neutral detergent fibre digestibility.

scholarly journals Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

2021 ◽  
Vol 51 (3) ◽  
pp. 399-406
Author(s):  
K. Selzer ◽  
A. Hassen ◽  
A.M. Akanmu ◽  
A.Z.M. Salem
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Poor Quality ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Forage Digestibility

Forages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibility.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

scholarly journals Ancient grains as novel dietary carbohydrate sources in canine diets

2021 ◽  
Author(s):  
Zachary T Traughber ◽  
Fei He ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Maria R C de Godoy
Keyword(s):  
Latin Square ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Nutrient Digestibility ◽  
Total Dietary Fiber ◽  
Carbohydrate Source ◽  
Beneficial Effects ◽  
Food Market ◽  
Dietary Treatments

Abstract Ancient grains are becoming an increasingly abundant carbohydrate source in the pet food market as a result of their popularity and novelty in the human market. Thus, it is imperative to evaluate the characteristics of these ingredients in vivo. Ten adult intact female beagles were used in a replicated 5x5 Latin square design. Five dietary treatments were evaluated containing either: rice (CON), amaranth (AM), white proso millet (WPM), quinoa (QU), or oat groats (OG). All diets were formulated to include 40% of the test grain and to be isonitrogenous, isocaloric, and nutritionally complete and balanced for adult dogs at maintenance. The objectives were (1) to evaluate the effects of the novel carbohydrate sources on total apparent total tract digestibility (ATTD), fecal microbiota, and fermentative end-product concentrations and (2) to evaluate the effects of novel carbohydrate sources on the post-prandial glycemic and insulinemic responses in healthy adult dogs. All diets were well-accepted by the dogs and fecal scores remained within ideal range for all treatments. In terms of ATTD, all diets were well-digested by the dogs; WPM had the highest digestibility of dry and organic matter in contrast with dogs fed the other treatments (P &lt; 0.05). Additionally, ATTD of total dietary fiber was highest for WPM (72.6%) in contrast with QU (63.5%) and CON (50.8%) but did not differ from AM (65.7%) and OG (66.6%). Dogs fed AM or OG had greater (P &lt; 0.05) fecal concentrations of total short-chain fatty acids, as well as propionate and butyrate concentrations, than CON. Ancient grain inclusion appears to beneficially shift fecal microbial populations, with increases in relative abundances of butyrogenic bacteria (i.e., members of the Lachnospiraceae family) observed for OG and reductions in Fusobacteriaceae for both AM and OG when compared with CON. Post-prandial glycemic and insulinemic responses did not differ among treatments. Together, these data suggest that ancient grains can be included up to 40% of the diet while eliciting beneficial effects on overall host health without detrimentally affecting nutrient digestibility.

In situ and in vitro techniques for estimating degradation parameters and digestibility of diets based on maize or sorghum

2020 ◽  
Vol 158 (1-2) ◽  
pp. 150-158 ◽  
Author(s):  
B. C. Silva ◽  
M. V. C. Pacheco ◽  
L. A. Godoi ◽  
F. A. S. Silva ◽  
D. Zanetti ◽  
...  
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
In Vitro Techniques ◽  
Sorghum Grains ◽  
Sorghum Silage ◽  
The Individual ◽  
Individual Grains

AbstractAn experiment was conducted to evaluate: (1) the effects of ensiling maize or sorghum grains after reconstitution on readily soluble fraction (a), potentially degradable fraction in the rumen (b) and rate constant for degradation of b (c) of dry matter (DM), organic matter (OM) and starch (STA); and (2) an appropriate incubation time for in situ or in vitro procedures to estimate in vivo digestibility. Four rumen-cannulated Nellore bulls (body weight = 262 ± 19.6 kg) distributed in a 4 × 4 Latin square were used. Diets were based on dry ground maize (DGM); or dry ground sorghum (DGS); or reconstituted ground maize silage; or reconstituted ground sorghum silage. In vitro and in situ incubations of the individual grains and diets were simultaneously performed with in vivo digestibility. In general, reconstituted grains and diets based on reconstituted grains presented greater (P < 0.05) fraction a and lower (P < 0.05) fraction b of DM, OM and STA compared to dry grains and diets based on dry grain. However, the magnitude of response of the reconstitution and ensiling process on DM and OM degradability parameter was greater for maize than that for sorghum. Moreover, no differences (P > 0.05) were observed between DGM- and DGS-based diets for c estimates. The results suggest that the reconstitution process promotes grains protein matrix breakdown increasing STA availability. The incubation times required for in vivo digestibility estimations of DM, OM and STA are 24 h for in situ and 36 h for in vitro procedures.

The effects of anAspergillus oryzaeextract containing alpha-amylase activity on ruminal fermentation and milk production in lactating Holstein cows

2005 ◽  
Vol 81 (3) ◽  
pp. 365-374 ◽  
Author(s):  
J. M. Tricarico ◽  
J. D. Johnston ◽  
K. A. Dawson ◽  
K. C. Hanson ◽  
K. R. McLeod ◽  
...  
Keyword(s):  
Milk Production ◽  
Amylase Activity ◽  
Latin Square ◽  
Alpha Amylase ◽  
Holstein Cows ◽  
Ruminal Fermentation ◽  
Nutrient Metabolism ◽  
Lactating Cows

AbstractThe effects of anAspergillus oryzaeextract containing alpha-amylase activity (Amaize™, Alltech Inc., Nicholasville, KY) were examinedin vivoandin vitro. A lactating cow study employed 20 intact and four ruminally fistulated Holstein cows in a replicated 4 × 4 Latin-square design to examine the effects of four concentrations of dietary Amaize™ extract on milk production and composition, ruminal fermentation and serum metabolite concentrations. The treatment diets contained 0, 240, 480 or 720 alpha-amylase dextrinizing units (DU) per kg of total mixed ration (TMR) (dry-matter basis). The supplemental alpha-amylase increased the yields of milk (P= 0·02), fat (P= 0·02) and protein (P= 0·06) quadratically. The maximum milk yield was obtained when 240 DU per kg of TMR were offered. Ruminalin situstarch disappearance was not affected by alpha-amylase supplementation in lactating cows or ruminally cannulated steers. Supplemental alpha-amylase extract reduced the molar proportion of propionate in the rumen of steers (P= 0·08) and lactating cows (P= 0·04), and in rumen-simulating cultures (P= 0·04). The supplement also increased the molar proportions of acetate (P= 0·06) and butyrate (P= 0·05), and the serum beta-hydroxybutyrate (P= 0·01) and non-esterified fatty acid (P= 0·03) concentrations in lactating cows. The improvements in milk production appear to be a consequence of the effects of alpha-amylase on ruminal fermentation and the potential changes in nutrient metabolism that result from them. We conclude that supplemental alpha-amylase may be given to modify ruminal fermentation and improve milk and component yield in lactating Holstein cattle.

Effect of a dried live yeast culture on in vivo apparent digestibility and on in vitro fibrolytic activity of large intestine fluid contents, in horses fed high fibre or high starch pelleted feeds

2000 ◽  
Vol 2000 ◽  
pp. 58-58
Author(s):  
B. Medina ◽  
D. Poillon ◽  
R. Power ◽  
V. Julliand
Keyword(s):  
Large Intestine ◽  
Nutrient Digestibility ◽  
Yeast Culture ◽  
Microbial Composition ◽  
Yeast Cultures ◽  
High Fibre ◽  
High Starch ◽  
Live Yeast

Performance horses are often fed very energetic diets including large amount of grains. Thus, a important quantity of soluble carbohydrate is bound to reach the hindgut, altering biochemical and microbial composition of the intestinal contents (Julliand et al., 1999) and leading to a wide variety of diseases like colic, laminitis and diarrhoea (Clarke et al., 1990). In high concentrate rations, live yeast cultures have been reported to stimulate specific groups of bacteria, and moderate large ruminal pH decreases (Williams et al., 1991). Therefore, this trial was designed to evaluate the effect of a live yeast culture preparation (Yea Sacc1026™) on the activity of the intestinal ecosystem (caecum and colon) of horses fed high fibre (HF) or high starch (HS) pelleted feeds. This current summary reports only the results of the nutrient digestibility in vivo and the ability in vitro of large intestine fluid contents to degrade straw.

EFFECT OF FORMALDEHYDE TREATMENT OF SOYBEAN MEAL ON RATES OF PROTEIN DEGRADATION AND MICROBIAL PROTEIN CONCENTRATION IN THE BOVINE RUMEN

1983 ◽  
Vol 63 (1) ◽  
pp. 181-190 ◽  
Author(s):  
S. MAHADEVAN ◽  
R. M. TEATHER ◽  
J. D. ERFLE ◽  
F. D. SAUER
Keyword(s):  
Soybean Meal ◽  
Latin Square ◽  
Experimental Period ◽  
Ammonia Concentration ◽  
Bovine Rumen ◽  
Rumen Degradation ◽  
Formaldehyde Treatment ◽  
Highly Correlated

Four rumen-fistulated cows were fed concentrate:corn silage (40: 60) diets in a 4 × 4 latin square design experiment. The diets differed in the soybean meal or soybean meal treated with 0.1, 0.3 or 0.6% (wt/wt) formaldehyde. Rumen contents were collected hourly from 0900 h to 1600 h with an additional sample at half an hour after the 1100 h feeding on 3 days in the second week of each 2-wk experimental period. The maximum ammonia concentration in rumen contents decreased with increasing levels of formaldehyde used to treat the soybean meal. From 1130 to 1200 h the rates of ammonia appearance in the rumen contents were different (P < 0.05) for all four diets. Treatment of soybean meal with between 0.1 and 0.3% (wt/wt) formaldehyde reduced its in vitro degradation by rumen protease by about 50% compared with the untreated control. The rates of in vivo ammonia appearance and in vitro proteolysis were highly correlated (r = 0.966, P < 0.01) between 1130 h and 1200 h. These rates of rumen ammonia appearance reflected the in vivo rate of proteolysis. Both bacterial and protozoal protein content decreased significantly at the higher levels of formaldehyde treatment. There was a negative correlation (r = − 0.63, P < 0.01) between protozoal and bacterial protein. Key words: Formaldehyde, soybean meal, bovine, rumen, degradation, protease

scholarly journals Inclusion of crude glycerin with different roughages changes ruminal parameters and in vitro gas production from beef cattle

2016 ◽  
Vol 46 (5) ◽  
pp. 889-894 ◽  
Author(s):  
Josimari Regina Paschoaloto ◽  
Jane Maria Bertocco Ezequiel ◽  
Marco Túlio Costa Almeida ◽  
Vanessa Ruiz Fávaro ◽  
Antonio Carlos Homem Junior ◽  
...  
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Corn Silage ◽  
Biodiesel Production ◽  
Crude Glycerin ◽  
Ruminal Fermentation ◽  
Nellore Cattle

ABSTRACT: The increasing availability of crude glycerin from biodiesel production has generated great stock in the industries. To solve this problem, crude glycerin is being used as an energy source to replace corn in livestock diets. This study evaluated the effects of the association of crude glycerin (10% on DM of diets) with different roughages in Nellore cattle diets on ruminal pH and ammonia, degradability, digestibility of dry matter and nutrients, and greenhouse gas production. Six ruminally cannulated Nellore steers were assigned to a 6×6 Latin square design. The following treatments were evaluated: Hydrolyzed Sugarcane associated or not with crude glycerin, Corn Silage associated or not with crude glycerin or Tifton-85 Hay associated or not with crude glycerin. Association of crude glycerin with roughages did not affect the rumen ammonia concentration and pH and dry matter intake, but reduced the intake of NDF for diets with Hydrolyzed Sugarcane and Corn Silage and reduced the digestibility of DM, OM, NDF, EE, CNF and starch and decreased the effective degradation at the rate of 8% h-1 for diets with Tifton-85 Hay. The association crude glycerin with Hydrolyzed Sugarcane reduced the production of CH4 and CO2 in mL g-1 of DM. The inclusion of crude glycerin affects differently nutrient utilization in diets with Corn Silage, Hydrolyzed Sugarcane or Tifton-85 hay. Moreover, promotes mitigation of greenhouse gases in diets with Hydrolyzed Sugarcane. Association of crude glycerin with Corn Silage in Nellore cattle diets showed better conditions of ruminal fermentation and utilization of nutrients.

scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

Cultivation of housefly larvae (Musca domestica L.) as a biological method to enrich rice straw and maximise its utilisation

2021 ◽  
pp. 1-12
Author(s):  
F.M. Abo-Donia ◽  
U.A. Nayel
Keyword(s):  
Rice Straw ◽  
Musca Domestica ◽  
Crude Protein ◽  
In Vivo Studies ◽  
Feed Consumption ◽  
Neutral Detergent Fibre ◽  
Biological Technique ◽  
Acid Detergent Fibre

This study is aimed at presenting a new biological technique to enrich rice straw via the cultivation of Musca domestica (MD) and at evaluating the outcome of this technique using in vitro and in vivo studies. Rice straw was treated using molasses and water at 17.5: 0.375: 7.0 (w/v/v), respectively. The mixture was left in an open shaded area for 11 successive days to allow MD to lay eggs and produce larvae. During this time, the recorded temperature was 35.6±2.2 °C and the humidity was 49±5.7%. Rice straw treated with 4% urea (URS) and untreated rice straw (RS) were used for comparison with in vitro and in vivo MD maggot rice straw (MRS). The crude protein (CP) and ether extract (EE) contents gradually increased during the first 8 days, and then began to gradually decrease until the 11th day. In vitro studies were performed on RS, MRS and URS as a single substrate. Instead, in the in vivo studies, experimental RS was offered ad libitum to sheep, and a concentrate feed mixture was provided as pellets to cover the maintenance requirements. The volume of produced gas and the in vitro degradability of the organic matter (OM), ash-free neutral detergent fibre, and CP (P<0.05) increased with the fermentation of the mg of MRS, compared to URS, which in turn was (P<0.05) higher than that of RS incubation. The consumed MRS was significantly (P<0.05) higher than the consumed URS. At the same time, the URS consumption was highly (P<0.05) significant, compared to the RS consumption. The eating, ruminating and chewing time (min/head/d) was significantly (P<0.05) higher when RS was consumed, compared to URS and MRS. Eating a diet including MRS led to higher (P<0.05) dry matter, OM, CP, EE, neutral detergent fibre, and acid detergent fibre digestibility, than the diet containing URS, which was significantly (P<0.05) superior to the diet containing RS. The total digestible nutrients (TDN) and digestible crude protein values of the MRS-containing diet improved (P<0.05) more than that of URS, which was significantly (P<0.05) higher than RS. In short, the results indicate that MD maggots could be produced on rice straw to elevate the protein content and nutritional value of feeds. Moreover, this technique improved the feed consumption and digestion coefficients more than the treated and untreated rice straw.

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