Molecular characterisation and expression profiling of calcineurin B-like (CBL) genes in Chinese cabbage under abiotic stresses

Calcium signals act as a second messenger in plant responses to various abiotic stresses, which regulate a range of physiological processes. Calcium-binding proteins, like calcineurin B-like (CBL) proteins, belong to a unique group of calcium sensors that play a role in calcium signalling. However, their identities and functions are unknown in Chinese cabbage. In this study, 17 CBL genes were identified from the Brassica rapa L. (Chinese cabbage) database and Br135K microarray datasets. They were used to construct a phylogenetic tree with known CBL proteins of other species. Analysis of genomic distribution and evolution revealed different gene duplication in Chinese cabbage compared to Arabidopsis. The microarray expression analysis showed differential expression of BrCBL genes at various temperatures. Organ-specific expression was observed by RT–PCR, and qRT–PCR analyses revealed responsiveness of BrCBL genes to cold, drought and salt stresses. Our findings confirm that CBL genes are involved in calcium signalling and regulate responses to environmental stimuli, suggesting this family gene have crucial role to play in plant responses to abiotic stresses. The results facilitate selection of candidate genes for further functional characterisation. In addition, abiotic stress-responsive genes reported in this study might be exploited for marker-aided backcrossing of Chinese cabbage.

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Characterization and abiotic stress-responsive expression analysis of SGT1 genes in Brassica oleracea

Genome ◽

10.1139/gen-2015-0128 ◽

2016 ◽

Vol 59 (4) ◽

pp. 243-251 ◽

Cited By ~ 9

Author(s):

Ashokraj Shanmugam ◽

Senthil Kumar Thamilarasan ◽

Jong-In Park ◽

Mi Young Jung ◽

Ill-Sup Nou

Keyword(s):

Brassica Oleracea ◽

Expression Analysis ◽

Abiotic Stresses ◽

Target Genes ◽

Expression Patterns ◽

Strong Association ◽

Stress Conditions ◽

Plant Responses ◽

Functional Domain ◽

Specific Expression

SGT1 genes are involved in enhancing plant responses to various biotic and abiotic stresses. Brassica oleracea is known to contain two types of SGT1 genes, namely suppressor of G2 allele of SKP1 and suppressor of GCR2. In this study, through systematic analysis, four putative SGT1 genes were identified and characterized in B. oleracea. In phylogenetic analysis, the genes clearly formed separate groups, namely BolSGT1a, BolSGT1b (both suppressor of G2 allele of SKP1 types), and BolSGT1 (suppressor of GCR2). Functional domain analysis and organ-specific expression patterns suggested possible roles for BolSGT1 genes during stress conditions. BolSGT1 genes showed significant changes in expression in response to heat, cold, drought, salt, or ABA treatment. Interaction network analysis supported the expression analysis, and showed that the BolSGT1a and BolSGT1b genes are strongly associated with co-regulators during stress conditions. However, the BolSGT1 gene did not show any strong association. Hence, BolSGT1 might be a stress resistance-related gene that functions without a co-regulator. Our results show that BolSGT1 genes are potential target genes to improve B. oleracea resistance to abiotic stresses such as heat, cold, and salt.

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Expression pattern of SLWRKY33 and SLERF5 in tomato plants under elevated salt concentration and water deficit

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v20.777 ◽

1970 ◽

pp. 266-270

Author(s):

H. R. Jafarov ◽

K. G. Gasimov

Keyword(s):

Water Deficit ◽

Expression Pattern ◽

Salt Concentration ◽

Abiotic Stresses ◽

Northern Hybridization ◽

Transcriptional Level ◽

Plant Responses ◽

Elevated Concentration ◽

Specific Expression ◽

Tomato Plants

Aim. High salinity, drought, and low temperature are the major abiotic stresses affecting plant growth and development and can lead to serious yield losses of agricultural crops. In plants, the majority of responses to abiotic stresses are con-trolled at the transcriptional level that is regulated by transcription factors (TFs). The family of WRKY and AP2/ERF are important superfamilies involved in response of plants to abiotic stress. Methods. The tissue-specific expression pattern of two superfamily members – SlWRKY33 from WRKY and SlERF5 from AP2/ERF were studied in tomato plants by means of Northern RNA hybridization and immunoblotting of total proteins. Results. Northern hybridization revealed very quick and strong expression of SlWRKY33 at elevated concentration of salt and water deficit, and rela-tively late and weaker expression of SlERF5 genes. Western hybridization with specific polyclonal antibodies revealed that during water deficit SlERF5 and SlWRKY33 were intensively expressed in variety of tissues of tomato plants. SlERF5 expressed in shoot apex, young leaves, roots and stems, while SlWRKY33 expressed in the same tissues as above and in axillary buds. Conclusions. Obtained results indicate that both SlWRKY33 and SlERF5 are involved in plant responses to abiotic stresses like elevated salt concentration and water deficit.Keywords: Solanum lycopersicum L., transcription factor, cloning, immunohybridization abiotic stresses.

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Calcium Sensors as Key Hubs in Plant Responses to Biotic and Abiotic Stresses

Frontiers in Plant Science ◽

10.3389/fpls.2016.00327 ◽

2016 ◽

Vol 7 ◽

Cited By ~ 122

Author(s):

Benoît Ranty ◽

Didier Aldon ◽

Valérie Cotelle ◽

Jean-Philippe Galaud ◽

Patrice Thuleau ◽

...

Keyword(s):

Abiotic Stresses ◽

Plant Responses ◽

Biotic And Abiotic Stresses ◽

Calcium Sensors

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Metabolomics-Guided Elucidation of Plant Abiotic Stress Responses in the 4IR Era: An Overview

Metabolites ◽

10.3390/metabo11070445 ◽

2021 ◽

Vol 11 (7) ◽

pp. 445

Author(s):

Morena M. Tinte ◽

Kekeletso H. Chele ◽

Justin J. J. van der Hooft ◽

Fidele Tugizimana

Keyword(s):

Machine Learning ◽

Abiotic Stress ◽

Stress Responses ◽

Abiotic Stresses ◽

Industrial Revolution ◽

Chemical Space ◽

Big Data Analytics ◽

Plant Responses ◽

Next Generation ◽

Computational Tools

Plants are constantly challenged by changing environmental conditions that include abiotic stresses. These are limiting their development and productivity and are subsequently threatening our food security, especially when considering the pressure of the increasing global population. Thus, there is an urgent need for the next generation of crops with high productivity and resilience to climate change. The dawn of a new era characterized by the emergence of fourth industrial revolution (4IR) technologies has redefined the ideological boundaries of research and applications in plant sciences. Recent technological advances and machine learning (ML)-based computational tools and omics data analysis approaches are allowing scientists to derive comprehensive metabolic descriptions and models for the target plant species under specific conditions. Such accurate metabolic descriptions are imperatively essential for devising a roadmap for the next generation of crops that are resilient to environmental deterioration. By synthesizing the recent literature and collating data on metabolomics studies on plant responses to abiotic stresses, in the context of the 4IR era, we point out the opportunities and challenges offered by omics science, analytical intelligence, computational tools and big data analytics. Specifically, we highlight technological advancements in (plant) metabolomics workflows and the use of machine learning and computational tools to decipher the dynamics in the chemical space that define plant responses to abiotic stress conditions.

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Characterization of the calcium-binding sites of calcineurin B

FEBS Letters ◽

10.1016/0014-5793(95)00207-p ◽

1995 ◽

Vol 362 (1) ◽

pp. 55-58 ◽

Cited By ~ 35

Author(s):

Lazaros T Kakalis ◽

Michael Kennedy ◽

Robert Sikkink ◽

Frank Rusnak ◽

Ian M Armitage

Keyword(s):

Binding Sites ◽

Calcium Binding ◽

Calcineurin B ◽

Calcium Binding Sites

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Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure

International Journal of Molecular Sciences ◽

10.3390/ijms22083835 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3835

Author(s):

Nicola Tempest ◽

Elizabeth Batchelor ◽

Christopher J. Hill ◽

Hannan Al-Lamee ◽

Josephine Drury ◽

...

Keyword(s):

Calcium Binding ◽

Binding Protein ◽

Embryo Implantation ◽

Calcium Signalling ◽

Premenopausal Women ◽

Subcellular Location ◽

Early Embryo ◽

Calcium Binding Protein ◽

Regulatory Function ◽

S100 Calcium Binding Protein

Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. We initially examined 20 full-thickness endometrial biopsies from premenopausal women across the menstrual cycle to characterize levels of AGR3 protein in each endometrial sub-region at the cellular level. A further 53 endometrial pipelle biopsies collected in the window of implantation were subsequently assessed to determine differential endometrial AGR3 and S100P levels relevant to RIF (n = 13) and RPL (n = 10) in comparison with parous women (n = 30) using immunohistochemistry. Significantly higher AGR3 and S100P immunostaining was observed in ciliated cells of the LE of women with recurrent reproductive failure compared with parous women, suggesting aberrant subcellular location-associated pathophysiology for these conditions. The nuclear localisation of S100P may allow transcriptional regulatory function, which is necessary for implantation of a viable pregnancy. Further work is thus warranted to assess their utility as diagnostic/therapeutic targets.

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The calcium-dependent ATP-Mg/Pi mitochondrial carrier is a target of glucose-induced calcium signalling in Saccharomyces cerevisiae

Biochemical Journal ◽

10.1042/bj20050806 ◽

2005 ◽

Vol 392 (3) ◽

pp. 537-544 ◽

Cited By ~ 37

Author(s):

Santiago Cavero ◽

Javier Traba ◽

Araceli Del Arco ◽

Jorgina Satrústegui

Keyword(s):

Calcium Binding ◽

Mitochondrial Protein ◽

Calcium Signalling ◽

Cytosolic Calcium ◽

Calcium Signal ◽

Transport Activity ◽

Wild Type ◽

Binding Motifs ◽

Mitochondrial Carrier ◽

Calcium Dependent

Sal1p is a mitochondrial protein that belongs to the SCaMC (short calcium-binding mitochondrial carrier) subfamily of mitochondrial carriers. The presence of calcium-binding motifs facing the extramitochondrial space allows the regulation of the transport activity of these carriers by cytosolic calcium and provides a new mechanism to transduce calcium signals in mitochondria without the requirement of calcium entry in the organelle. We have studied its transport activity, finding that it is a carboxyatractyloside-resistant ATP-Mg carrier. Mitochondria from a disruption mutant of SAL1 have a 50% reduction in the uptake of ATP. We have also found a clear stimulation of ATP-transport activity by calcium, with an S0.5 of approx. 30 μM. Our results also suggest that Sal1p is a target of the glucose-induced calcium signal which is non-essential in wild-type cells, but becomes essential for transport of ATP into mitochondria in yeast lacking ADP/ATP translocases.

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