Rhythmic patterns of nutrient acquisition by wheat roots

2002 ◽  
Vol 29 (5) ◽  
pp. 595 ◽  
Author(s):  
Sergey Shabala ◽  
Andrew Knowles
Keyword(s):  
Root Zone ◽  
Root Hairs ◽  
Rhythmic Activity ◽  
Root Apex ◽  
Root Surface ◽  
Ion Flux ◽  
Nutrient Acquisition ◽  
Wheat Roots ◽  
Non Invasive ◽  
Functional Zone

Oscillatory patterns in H+, K+, Ca2+ and Cl- uptake were observed at different regions of the root surface, including root hairs, using a non-invasive ion flux measuring technique (the MIFE™ technique). To our knowledge, this is the first report of ultradian oscillations in nutrient acquisition in the mature root zone. Oscillations of the largest magnitude were usually measured in the elongation region, 2–4 mm from the root apex. There were usually at least two oscillatory components present for each ion measured: fast, with periods of several minutes; and slow, with periods of 50–80 min. Even within the same functional zone, the periods of ion flux oscillations were significantly different, suggesting that they are driven by some internal mechanisms located in each cell rather than originating from one ‘central clock pacemaker’. There were also significant changes in the oscillatory characteristics (both periods and amplitudes) of fluxes from a single small cluster of cells over time. Analysis of phase shifts between oscillations in different ions suggested that rhythmic activity of a plasma membrane H+-pump may be central to observed rhythmic nutrient acquisition by plant roots. We discuss the possible adaptive significance of such an oscillatory strategy for root nutrient acquisition.

Effectiveness of complex inoculation of spring wheat with N[2] -fi xing bacteria Azospirillum brasilense and mold-antagonist Chaetomium cochliodes

2014 ◽  
Vol 1 (3) ◽  
pp. 57-61
Author(s):  
E. Kopylov
Keyword(s):  
Spring Wheat ◽  
Root Zone ◽  
Wheat Root ◽  
Root Surface ◽  
Atmospheric Nitrogen ◽  
Rhizospheric Soil ◽  
Chain Reaction ◽  
Wheat Roots ◽  
Chlorophyll A And B ◽  
Polymerase Chain

Aim. To study the specifi cities of complex inoculation of spring wheat roots with the bacteria of Azospirillum genus and Chaetomium cochliodes Palliser 3250, and the isolation of bacteria of Azospirillum genus, capable of fi xing atmospheric nitrogen, from the rhizospheric soil, washed-off roots and histoshere. Materials and meth- ods. The phenotypic features of the selected bacteria were identifi ed according to Bergi key. The molecular the polymerase chain reaction and genetic analysis was used for the identifi cation the bacteria. Results. It has been demonstrated that during the introduction into the root system of spring wheat the strain of A. brasilensе 102 actively colonizes rhizospheric soil, root surface and is capable of penetrating into the inner plant tissues. Conclusions. The soil ascomucete of C. cochliodes 3250 promotes better settling down of Azospirillum cells in spring wheat root zone, especially in plant histosphere which induces the increase in the content of chlorophyll a and b in the leaves and yield of the crop.

Cytological and histochemical characteristics of the axenic root surface of Alnus glutinosa

1986 ◽  
Vol 64 (10) ◽  
pp. 2216-2226 ◽  
Author(s):  
Yves Prin ◽  
Mireille Rougier
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Root Hairs ◽  
Root Apex ◽  
Alnus Glutinosa ◽  
Root Surface ◽  
Infection Process ◽  
Root Cap ◽  
A Cell ◽  
Histochemical Tests

The aim of the present study was to investigate the Alnus root surface using seedlings grown axenically. This study has focused on root zones where infection by the symbiotic actinomycete Frankia takes place. The zones examined extend from the root cap to the emerging root hair zone. The root cap ensheaths the Alnus root apex and extends over the root surface as a layer of highly flattened cells closely appressed to the root epidermal cell wall. These cells contain phenolic compounds as demonstrated by various histochemical tests. They are externally bordered by a thin cell wall coated by a thin mucilage layer. The root cap is ruptured when underlying epidermal cells elongate, and cell remnants are still found in the emerging root hair zone. Young emerging root hairs are bordered externally by a cell wall covered by a thin mucilage layer which reacts positively to the tests used for the detection of polysaccharides, glycoproteins, and anionic sites. The characteristics of the Alnus root surface and the biological function of mucilage and phenols present at the root surface are discussed in relation to the infection process.

Chemical and Physical Modification of the Rhizosphere

2000 ◽  
Author(s):  
Peter B. Tinker ◽  
Peter Nye
Keyword(s):  
Root Hairs ◽  
Soil Surface ◽  
Fine Sand ◽  
Root Surface ◽  
Root Penetration ◽  
Wheat Roots ◽  
Order Of Magnitude ◽  
Seminal Roots ◽  
Dry Soil ◽  
Living Organisms

The term ‘rhizosphere’ tends to mean different things to different people. In discussing how a root affects the soil, it is well to bear in mind the spread of the zone being exploited for a particular solute: if this is wide, there may be no point in emphasizing effects close to the root; but if it is narrow, predictions based on the behaviour of the bulk soil may be wide of the mark. In a moist loam after 10 days, a simple non-adsorbed solute moves about 1 cm, but a strongly adsorbed one will move about 1 mm. In a dry soil, the spread may be an order of magnitude less. The modifications to the soil in the rhizosphere may be physical, chemical or microbiological. In this chapter, we discuss essentially non-living modifications, and in chapter 8 the modifications that involve living organisms and their effects. Roots tend to follow pores and channels that are not much less, and are often larger, in diameter than their own. If the channels are larger, the roots are not randomly arranged in the void (Kooistra et al. 1992), but tend to be held against a soil surface by surface tension, and to follow the channel geotropically on the down-side. If the channels are smaller, good contact is assured, but the roots do not grow freely unless some soil is displaced as the root advances. For example, in winter wheat, Low (1972) cites minimum pore sizes of 390–450 μm for primary seminal roots, 320–370 μm for primary laterals, 300–350 μm for secondary laterals, and 8–12 μm for root hairs, though some figures seem large. Whiteley & Dexter (1984) and Dexter (1986a, b, c) have studied the mechanics of root penetration in detail (section 9.3.5). It may compact and reorient the soil at the root surface. Greacen et al. (1968) found that wheat roots penetrating a uniform fine sand increased the density only from 1.4 to 1.5 close to the root; and a pea radicle, a comparatively large root, raised the density of a loam from 1.5 to 1.55.

Structure and ontogeny of Betula alleghaniensis – Pisolithus tinctorius ectomycorrhizae

1990 ◽  
Vol 68 (3) ◽  
pp. 579-593 ◽  
Author(s):  
H. B. Massicotte ◽  
R. L. Peterson ◽  
C. A. Ackerley ◽  
L. H. Melville
Keyword(s):  
Root Hairs ◽  
Root Surface ◽  
Pisolithus Tinctorius ◽  
Initial Contact ◽  
Broad Host Range ◽  
Betula Alleghaniensis ◽  
Cortical Cells ◽  
Structural Modifications ◽  
Hartig Net ◽  
Primary Roots

The ontogeny and ultrastructure of ectomycorrhizae synthesized between Betula alleghaniensis (yellow birch) and Pisolithus tinctorius, a broad host range fungus, were studied to determine the structural modifications in both symbionts during ectomycorrhiza establishment. A number of stages, including initial contact of hyphae with the root surface, early mantle formation, and mature mantle formation, were distinguished. Interactions between hyphae and root hairs were frequent. As a paraepidermal Hartig net developed, root epidermal cells elongated in a radial direction, but wall ingrowths were not formed. Repeated branching of Hartig net hyphae resulted in extensive fine branches and the compartmentalization of hyphal cytoplasm. Nuclei and elongated mitochondria were frequently located in the narrow cytoplasmic compartments, and [Formula: see text] thickenings developed along walls of cortical cells in primary roots.

scholarly journals Host Specificity In The Root Hair "Curling Factor" of Rhizobium Spp.

1969 ◽  
Vol 22 (2) ◽  
pp. 413 ◽  
Author(s):  
Phaik Y Yao ◽  
JM Vincent
Keyword(s):  
Medicago Sativa ◽  
Host Specificity ◽  
Root Hair ◽  
Root Zone ◽  
Root Hairs ◽  
Degree Of Deformation ◽  
Medicago Sativa L ◽  
Rhizobium Spp ◽  
Hair Curling ◽  
Root Hair Curling

Thirty-eight cultures of rhizobia and 10 non-rhizobia growing in the root zone of clover (Trifolium glomeratum L.), 5 rhizobia and 3 non-rhizobia in that of lucerne (Medicago sativa L.), and 8 rhizobia in that ofSiratro (Phaseolus atropurpureus DO.) revealed a specific relationship between bacteria and host that determined the kind and degree of deformation of the root hairs.

scholarly journals Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass

Proteomes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Zhujia Ye ◽  
Sasikiran Reddy Sangireddy ◽  
Chih-Li Yu ◽  
Dafeng Hui ◽  
Kevin Howe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drought Stress ◽  
Molecular Mechanisms ◽  
Root Zone ◽  
Root Apex ◽  
Root Tip ◽  
Leaf Water Content ◽  
Severe Drought ◽  
Proteomics Data ◽  
Drought Treatment

Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, the leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. The root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), after one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially accumulated proteins (DAPs). From RT1 tissues, 6156, 7687, and 7699 proteins were quantified, and 296, 535, and 384 DAPs were identified in the SDT, D1W, and D3W samples, respectively. From RT2 tissues, 7382, 7255, and 6883 proteins were quantified, and 393, 587, and 321 proteins DAPs were identified in the SDT, D1W, and D3W samples. Between RT1 and RT2 tissues, very few DAPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DAPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DAPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to select proteins (genes) as better defined targets for developing drought tolerant plants. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD017441.

scholarly journals Local auxin biosynthesis acts downstream of brassinosteroids to trigger root foraging for nitrogen

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhongtao Jia ◽  
Ricardo F. H. Giehl ◽  
Nicolaus von Wirén
Keyword(s):  
Lateral Roots ◽  
Root Surface ◽  
Nutrient Acquisition ◽  
Fine Tuning ◽  
Auxin Biosynthesis ◽  
N Availability ◽  
Hormonal Crosstalk ◽  
Soil Exploration ◽  
N Deficiency ◽  
Coding Variants

AbstractLateral roots (LRs) dominate the overall root surface of adult plants and are crucial for soil exploration and nutrient acquisition. When grown under mild nitrogen (N) deficiency, flowering plants develop longer LRs to enhance nutrient acquisition. This response is partly mediated by brassinosteroids (BR) and yet unknown mechanisms. Here, we show that local auxin biosynthesis modulates LR elongation while allelic coding variants of YUCCA8 determine the extent of elongation under N deficiency. By up-regulating the expression of YUCCA8/3/5/7 and of Tryptophan Aminotransferase of Arabidopsis 1 (TAA1) under mild N deficiency auxin accumulation increases in LR tips. We further demonstrate that N-dependent auxin biosynthesis in LRs acts epistatic to and downstream of a canonical BR signaling cascade. The uncovered BR-auxin hormonal module and its allelic variants emphasize the importance of fine-tuning hormonal crosstalk to boost adaptive root responses to N availability and offer a path to improve soil exploration by expanded root systems in plants.

scholarly journals Mannose inhibition of nitrate uptake by wheat roots

2011 ◽  
Vol 79 (2) ◽  
pp. 107-110
Author(s):  
Jin Zhi Li ◽  
William John Cram ◽  
Guang Yuan He
Keyword(s):  
Triticum Aestivum ◽  
Cell Death ◽  
Steady State ◽  
Programmed Cell Death ◽  
Nitrate Uptake ◽  
Atp Depletion ◽  
Invasive Technique ◽  
Wheat Roots ◽  
Control Value ◽  
Non Invasive

The effect of mannose on nitrate uptake was investigated by a new non-invasive technique. Under normal condition, nitrate uptake by wheat (<em>Triticum aestivum</em> L.) roots was about 1-7 µmol gfwt<sup>-1</sup> h<sup>-1</sup>. After the addition of 10 mM mannose, net nitrate influx by wheat roots started to decrease and reached a new steady state at -40 ±50% of the control value after 73 min. After mannose supplied for 2 h, its removal caused net nitrate influx to be recovered to an original rate. Therefore, the inhibition of mannose on nitrate uptake is not due to the onset of programmed cell death because it starts too rapidly and it is reversible, however, it is probably due to Pi and consequent ATP depletion.

Early signalling processes in roots play a crucial role in the differential salt tolerance in contrasting Chenopodium quinoa accessions

2021 ◽  
Author(s):  
Nadia Bazihizina ◽  
Federico Vita ◽  
Raffaella Balestrini ◽  
Claudia Kiferle ◽  
Stefania Caparrotta ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Root Zone ◽  
Regulatory Protein ◽  
Membrane Integrity ◽  
Chenopodium Quinoa ◽  
Root Apex ◽  
Tolerance Mechanisms ◽  
Bladder Cell ◽  
Receptor Kinases ◽  
Root Plasma Membrane

Abstract Significant variation in epidermal bladder cell (EBC) density and salt tolerance (ST) exists amongst quinoa accessions, suggesting that salt sequestration in EBCs is not the only mechanism conferring ST in this halophyte. In order to reveal other traits that may operate in tandem with salt sequestration in EBCs and whether these additional tolerance mechanisms acted mainly at the root or shoot level, two quinoa (Chenopodium quinoa) accessions with contrasting ST and EBC densities (Q30, low ST with high EBC density versus Q68, with high ST and low EBC density) were studied. The results indicate that responses in roots, rather than in shoots, contributed to the greater ST in the accession with low EBC density. In particular, the tolerant accession had improved root plasma membrane integrity and K+ retention in the mature root zone in response to salt. Furthermore, superior ST in the tolerant Q68 was associated with faster and root-specific H2O2 accumulation and reactive oxygen species-induced K+ and Ca2+ fluxes in the root apex within 30 min after NaCl application. This was found to be associated with the constitutive up-regulation of the membrane-localized receptor kinases regulatory protein FERONIA in the tolerant accession. Taken together, this study shows that differential root signalling events upon salt exposure are essential for the halophytic quinoa; the failure to do this limits quinoa adaptation to salinity, independently of salt sequestration in EBCs.

Non-invasive estimation of root zone soil moisture from coarse root reflections in ground-penetrating radar images

2019 ◽  
Vol 436 (1-2) ◽  
pp. 623-639 ◽  
Author(s):  
Xinbo Liu ◽  
Xihong Cui ◽  
Li Guo ◽  
Jin Chen ◽  
Wentao Li ◽  
...  
Keyword(s):  
Soil Moisture ◽  
Ground Penetrating Radar ◽  
Root Zone ◽  
Coarse Root ◽  
Radar Images ◽  
Non Invasive ◽  
Ground Penetrating
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