Major plasma proteins in pig serum during postnatal development

2005 ◽  
Vol 17 (4) ◽  
pp. 439 ◽  
Author(s):  
M. Martin ◽  
M. A. Tesouro ◽  
N. González-Ramón ◽  
A. Piñeiro ◽  
F. Lampreave
Keyword(s):  
Acute Phase ◽  
Plasma Proteins ◽  
Time Course ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Serum Levels ◽  
Acid Glycoprotein ◽  
Newborn Pigs ◽  
Low Levels

The time-course of changes in the levels of albumin, α-fetoprotein (AFP), α1-protease inhibitor (α1-antitrypsin), α1-acid glycoprotein, fetuin, haptoglobin, transferrin, IgG and the major acute-phase protein (Pig-MAP) in the blood sera of pigs during the first days and weeks of life was investigated by quantitative radial immunodiffusion. The serum of newborn pigs before suckling was characterised by a very low concentration of total proteins (approximately 25 mg mL–1), low levels of albumin and transferrin and the lack of immunoglobulins. In contrast, α1-acid glycoprotein and fetuin are present at high levels (approximately 12 and 5 mg mL–1 respectively). The results of the present study show that the piglets undergo a very rapid metabolic maturation with regard to serum proteins, evolving from a characteristic ‘fetal’ pattern to an ‘adult’ one. We have paid special attention to the evolution of haptoglobin and Pig-MAP, which are two important acute-phase proteins in pigs. The evolution of serum levels of these proteins suggests that piglets must overcome a moderate acute-phase situation during the first week of life.

scholarly journals Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea

2016 ◽  
Vol 68 (4) ◽  
pp. 865-872 ◽  
Author(s):  
T.G. Rocha ◽  
F.D.F. Silva ◽  
C. Bortoletto ◽  
D.G. Silva ◽  
M.G. Buzinaro ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Least Square ◽  
Serum Concentrations ◽  
Fecal Samples ◽  
Acid Glycoprotein ◽  
Group B ◽  
Rotavirus Diarrhea

ABSTRACT The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and α1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves.

scholarly journals ACUTE PHASE PROTEIN INCREASE IN HIGH ALTITUDE MOUNTAINEERS

2017 ◽  
Vol 23 (6) ◽  
pp. 455-459
Author(s):  
Tolga Saka ◽  
Mehmet Karakus
Keyword(s):  
High Altitude ◽  
Acute Phase ◽  
Biochemical Parameters ◽  
Creatine Phosphokinase ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Serum Levels ◽  
Mountain Climbing ◽  
Sedentary Group ◽  
Hematological And Biochemical Parameters

ABSTRACT Introduction: Many middle-aged Turks go hiking in mountains to breathe some fresh air or to maintain fitness. Objective: This study investigated the effects of regular high altitude mountain climbing on the metabolic and hematological responses of mountaineers. Methods: Hematological and biochemical parameters were studied, as well as some hormonal values of 21 mountaineers and 16 healthy age-matched sedentary volunteers. Results: The neutrophil to lymphocyte ratio (NLR) was significantly lower (p<0.04) in mountaineers compared with the sedentary group. Total protein (p<0.001) and albumin (p<0.001) were lower, while the levels of ferritin (p<0.04), creatine (p<0.03) and creatine phosphokinase (p<0.01) were higher in mountaineers. Other hematological and biochemical parameters, i.e., erythrocytes, leukocytes, hemoglobin and hematocrit, did not change significantly. Conclusion: Our results show that regular exposure to high altitude increased the serum levels of some acute phase proteins with anti-inflammatory properties.

Studies on Acute Phase Proteins of Rat Serum. V. Effect of Induced Inflammation on the Synthesis of Albumin and α1-Acid Glycoprotein by Liver Slices

1975 ◽  
Vol 53 (4) ◽  
pp. 401-414 ◽  
Author(s):  
J. C. Jamieson ◽  
K. E. Morrison ◽  
D. Molasky ◽  
B. Turchen
Keyword(s):  
Acute Phase ◽  
Polypeptide Chain ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Rat Serum ◽  
Acid Glycoprotein ◽  
Liver Slices ◽  
Microsome Fraction ◽  
Lower Capacity ◽  
Acute Phase Serum

Liver slices from normal rats and those suffering from inflammation for 24–48 h were incubated with L-[14C]leucine or D-[I4C]glucosamine. Immunological techniques coupled with radioautography indicated that the microsome fraction prepared from slices contained the subcellular site of synthesis of the polypeptide chain of serum albumin, and the polypeptide and carbohydrate chains of α1-acid glycoprotein; both proteins were also present in the medium in labelled forms. The contents of albumin and α1-acid glycoprotein in the medium and in extracts of liver from experiments with liver slices from control rats and 8–72 h experimental rats were determined using the quantitative precipitin technique. There was a net increase in synthesis of both proteins when slices from control and experimental animals were used, the increase showing up in medium proteins. However, slices from livers from 8–72 h experimental rats had a greater capacity for synthesis of α1-acid glycoprotein and a lower capacity for synthesis of albumin than slices from livers from control rats, the greatest changes occurring with slices from 24 h experimental rats. Changes in synthetic capacities of liver slices from experimental rats for albumin and α1-acid glycoprotein were always accompanied by large increases in specific radioactivities of total medium proteins when experiments involved incubation of slices with L-[3H]leucine and D-[14C]glucosamine. It is suggested that the increase in specific radioactivities of medium proteins following incubation of liver slices from experimental rats with labelled leucine and glucosamine is a characteristic of the response of liver to inflammation, and reflects changes in the capacity of liver for the synthesis of α1-acid glycoprotein and other acute phase serum proteins.

Acute Phase Proteins and Interleukin?6 Serum Levels in Patients with Chronic Arterial Occlusion

2006 ◽  
Vol 762 (1) ◽  
pp. 493-495 ◽  
Author(s):  
WACLAW MAJEWSKI ◽  
RYSZARD STANISZEWSKI ◽  
ARTUR SLUPIANEK ◽  
ALEKSANDER GORNY ◽  
ANDRZEJ MACKIEWICZ
Keyword(s):  
Acute Phase ◽  
Interleukin 6 ◽  
Acute Phase Proteins ◽  
Arterial Occlusion ◽  
Serum Levels ◽  
Chronic Arterial Occlusion

scholarly journals Experimental Ehrlichia canis infection changes acute-phase proteins

2012 ◽  
Vol 21 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Thiago Demarchi Munhoz ◽  
Joice Lara Maia Faria ◽  
Giovanni Vargas-Hérnandez ◽  
José Jurandir Fagliari ◽  
Áureo Evangelista Santana ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Ehrlichia Canis ◽  
Laboratory Findings ◽  
Early Detection Of Disease ◽  
Acid Glycoprotein ◽  
The Third ◽  
Canine Ehrlichiosis

Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.

scholarly journals Plasma Hepcidin Correlates Positively With Interleukin-6 in Patients Undergoing Pulmonary Endarterectomy

2011 ◽  
pp. 493-502 ◽  
Author(s):  
P. MARUNA ◽  
M. VOKURKA ◽  
J. LINDNER
Keyword(s):  
Acute Phase ◽  
Iron Metabolism ◽  
Time Course ◽  
Acute Phase Proteins ◽  
Human Subjects ◽  
Circulatory Arrest ◽  
Experimental Studies ◽  
Acute Phase Reactant ◽  
Deep Hypothermic Circulatory Arrest ◽  
Pulmonary Endarterectomy

Hepcidin, a recently discovered antimicrobial peptide synthesized in the liver, was identified to be the key mediator of iron metabolism and distribution. Despite our knowledge of hepcidin increased in recent years, there are only limited data on hepcidin regulation during systemic inflammatory response in human subjects. In a prospective study, the time course of plasma hepcidin was analyzed in relations to six inflammatory parameters – plasma cytokines and acute-phase proteins in patients undergoing uncomplicated pulmonary endarterectomy. Twenty-four patients (males, aged 52.6±10.2 years, treated with pulmonary endarterectomy in a deep hypothermic circulatory arrest) were enrolled into study. Hepcidin, interleukin (IL)-6, IL-8, tumor necrosis factor-α, C-reactive protein, α1-antitrypsin and ceruloplasmin arterial concentrations were measured before surgery and repeatedly within 120 h post-operatively. Hemodynamic parameters, hematocrit and markers of iron metabolism were followed up. In a postoperative period, hepcidin increased from preoperative level 8.9 ng/ml (6.2-10.7) (median and interquartile range) to maximum 16.4 ng/ml (14.1-18.7) measured 72 h after the end of surgery. Maximum post-operative concentrations of hepcidin correlated positively with maximum IL-6 levels. Both hepcidin and IL-6 maximum concentrations correlated positively with extracorporeal circulation time. In conclusions, the study demonstrated that plasma hepcidin is a positive acute-phase reactant in relation to an uncomplicated large cardiac surgery. Hepcidin increase was related to IL-6 concentrations and to the duration of surgical procedure. Our clinical findings are in conformity with recent experimental studies defining hepcidin as a type II acute-phase protein.

Abstract 1629: Genetic Polymorphism A1675G On Angiotensin Receptor Type 2, Is Implicated In The Expression Of Acute Phase Proteins And The Development Of Coronary Atherosclerosis In Hypertensive Patients

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Dimitris Tousoulis ◽  
Nikolas Koumallos ◽  
Charalambos Antoniades ◽  
Despina Kardara ◽  
Alexis S Antonopoulos ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Arterial Hypertension ◽  
Acute Phase ◽  
Coronary Atherosclerosis ◽  
Acute Phase Proteins ◽  
Renin Angiotensin System ◽  
Serum Levels ◽  
Hypertensive Patients ◽  
The Impact

Introduction: Renin-angiotensin system affects cardiovascular disease pathogenesis through a balance of angiotensin II effects on proatherogenic constitutive type 1 and antiatherogenic inducible type 2 (AT2R) receptors. The impact of A1675G polymorphism on the development of hypertension and advanced atherosclerosis is controversial. We examined the impact of A1675G polymorphism on AT2R, on the risk for arterial hypertension and coronary atherosclerosis, and its effect on the expression of proatherogenic inflammatory molecules. Methods. The study population consisted of 310 males: 145 with arterial hypertension and 165 controls, matched for age and risk factors for atherosclerosis. Among hypertensive subjects, 37 had angiographically documented coronary atherosclerosis and 108 had no evidence of atherosclerosis. The presence of A1675G polymorphism on AT2R gene (located in chromosome X) was determined by PCR. Serum levels of C-reactive protein and fibrinogen was measured in all the participants. Results. The frequency of the A allele was similar between patients with arterial hypertension (64/145, 44.1%) and non-hypertensive subjects (73/165, 44.2%, p=NS), while the risk for arterial hypertension was OR[95%CI]:1.004[0.641–1.574], p=0.985 for the G vs A carriers. However, the risk for coronary atherosclerosis within the group of hypertensive subjects was significantly elevated in the carriers of the A allele (OR[95%CI]:2.128[1.003–4.513], p=0.04 vs carriers of the G allele). Importantly, the presence of the A allele was also associated with significantly higher levels of CRP (4.8±0.8mg/dl) compared to the carriers of the G allele (3.0±0.3mg/dl, p<0.05). Similarly, fibrinogen levels were higher in A-allele carriers (median(25 th –75th percentile) 395(340 – 455) mg/ml) compared to G-allele carriers (369(320 – 406) mg/ml, p<0.05). Conclusions: Although genetic polymorphism A1675G on AT2R is not associated with the development of arterial hypertension, it affects the risk for coronary artery disease among hypertensive patients. The presence of the A allele also leads to higher levels of CRP and fibrinogen, implying that this polymorphism may induce atherogenesis by modulating acute phase response in hypertensive individuals.

Sign in / Sign up

Export Citation Format

Share Document