scholarly journals Experimental Ehrlichia canis infection changes acute-phase proteins

2012 ◽  
Vol 21 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Thiago Demarchi Munhoz ◽  
Joice Lara Maia Faria ◽  
Giovanni Vargas-Hérnandez ◽  
José Jurandir Fagliari ◽  
Áureo Evangelista Santana ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Ehrlichia Canis ◽  
Laboratory Findings ◽  
Early Detection Of Disease ◽  
Acid Glycoprotein ◽  
The Third ◽  
Canine Ehrlichiosis

Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.

scholarly journals Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs

2011 ◽  
Vol 20 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Joice Lara Maia Faria ◽  
Thiago Demarchi Munhoz ◽  
Carolina Franchi João ◽  
Giovanny Vargas-Hernández ◽  
Marcos Rogério André ◽  
...  
Keyword(s):  
Acute Phase ◽  
Ehrlichia Canis ◽  
Post Inoculation ◽  
Doxycycline Hyclate ◽  
Blood Leukocytes ◽  
Canine Ehrlichiosis ◽  
Tnf Α ◽  
Study Results ◽  
Splenic Cells ◽  
Ifn Γ

Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-α, IL-10, IFN-γ, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-α 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-γ 18 and 30 days post-inoculation, respectively. This study suggests that TNF-α plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia.

scholarly journals Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

1986 ◽  
Vol 32 (5) ◽  
pp. 743-747 ◽  
Author(s):  
J Braun ◽  
T Schultek ◽  
K F Tegtmeier ◽  
A Florenz ◽  
C Rohde ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Coefficients Of Variation ◽  
Alpha 1 Antitrypsin ◽  
Heel Prick ◽  
Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

scholarly journals Concentrations of acute-phase proteins and immunoglobulins in serum and synovial fluid in clinically healthy heifers and steers

2019 ◽  
Vol 39 (6) ◽  
pp. 388-392
Author(s):  
Paula A. Di Filippo ◽  
Saulo T. Lannes ◽  
Marcos A.D. Meireles ◽  
Andressa F.S. Nogueira ◽  
Célia R. Quirino
Keyword(s):  
Synovial Fluid ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
Crossbred Cattle ◽  
Acid Glycoprotein ◽  
Molecular Weights ◽  
Sds Page ◽  
Radiocarpal Joint ◽  
Statistical Program

ABSTRACT: The aim of the study was to determine the concentration pattern of intra-articular acute phase proteins (APPs) and immunoglobulins in healthy crossbred cattle. Synovial fluid (SF) samples were collected from the radiocarpal joint of 25 heifers and 25 steers. Concentrations of APPs were measured by SDS-PAGE. The results were submitted to analysis of variance using the SAS statistical program, and means were compared by the Student-Newman-Keuls test (P<0.05). Thirty-seven proteins with molecular weights ranging from 7 to 37kDa were identified in SF of all animals. Eight were nominally identified with immunoglobulin A (IgA) and G (IgG), ceruloplasmin (Cp), transferrin (Tf), albumin (Ab), α1-antitripsin (AAT), α1-acid glycoprotein (AGP), and haptoglobin (Hp). The α1-antitripsin was only identified in the Sf of the heifers. The SF values of Cp, Hp, AGP and IgA were significantly higher in heifers than in steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, however the α1-antitrypsin was identified only in SF. The serum values Tf, AGP and IgG were significantly higher in heifers compared with steers. In conclusion, the physiological acute-phase proteins concentrations in synovial fluid of healthy ruminants can be useful in the interpretation of samples from animals with joint diseases. The SF electrophoretic profile of healthy ruminants differs depending on gender. Similar proteins were nominally identified in the sera, but only the SF of α1-antitrypsin.

scholarly journals Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea

2016 ◽  
Vol 68 (4) ◽  
pp. 865-872 ◽  
Author(s):  
T.G. Rocha ◽  
F.D.F. Silva ◽  
C. Bortoletto ◽  
D.G. Silva ◽  
M.G. Buzinaro ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Least Square ◽  
Serum Concentrations ◽  
Fecal Samples ◽  
Acid Glycoprotein ◽  
Group B ◽  
Rotavirus Diarrhea

ABSTRACT The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and α1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves.

RESULT OF STUDYING SOME ACUTE PHASE PROTEINS AND CORTISOL IN PREGNANT EWES

2017 ◽  
Vol 19 (3) ◽  
pp. 27-31
Author(s):  
Ulaankhuu A. ◽  
Lkhamjav G. ◽  
Yoshio Nakamura ◽  
Zolzaya M.
Keyword(s):  
Temperature Stress ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Cold Temperature ◽  
Cold Winter ◽  
Acid Glycoprotein

One of the specific characteristics of Mongolian livestock is that parturition occurs during the cold winter and spring seasons. Aim of our study is to investigate acute phase proteins (APP) in pregnant ewes during the time of cold temperature stress. According to the study, haptoglobin (Hp) concentration in serum of pregnant ewes was highest in February, as well as high variation of Hp was detected during the remaining months. In addition, alpha -1 acid glycoprotein (α1AGР) was also highest in February, but decreased gradually in the following months.

Studies on Biomarkers of Hepatic Lipidosis in Transition Cows with Special Reference to Liver Ultrasonograhy, Liver Specific Enzymes and Acute Phase Proteins

2020 ◽  
Author(s):  
Randhir Singh ◽  
S. N.S. Randhawa ◽  
C. S. Randhawa ◽  
Sushma Chhabra ◽  
Naimi Chand
Keyword(s):  
Early Diagnosis ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Gamma Glutamyl Transferase ◽  
Hepatic Lipidosis ◽  
Plasma Urea ◽  
Amyloid A ◽  
Total Plasma Protein ◽  
Glutamyl Transferase ◽  
Liver Ultrasonography

Background: Use of novel biomarkers is the need of hour for prediction and early diagnosis of bovine production diseases such as hepatic lipidosis (HL). Liver ultrasonography and estimation of liver specific enzymes activities have been successfully used for diagnosis of HL. However, use of inflammatory mediator like tumor necrosis factor-á (TNF–á) or acute phase proteins (APPs) like C reactive protein (CRP), serum amyloid A (SAA) and haptoglobin (Hp) as biomarkers of HL has not been elucidated. The present study was therefore designed to evaluate liver ultrasonography and serum APPs concentrations as early predictive biomarkers of bovine HL. Methods: This study included one hundred one (101) multiparous crossbred cows in advanced pregnancy from dairy farm in Punjab, India. The cows were grouped according to their transition stage i.e., Far off dry (FOD), close up dry (CUD) and Fresh (F). Liver ultrasonography along with hemato-biochemical analysis and estimation of acute phase proteins was carried out during different stages of transition period. Result: This study included 101 cows, of which 71 (70.3%) had ultrasonographic features of normal liver and 30 (29.7%) had ultrasonographic features of HL (Grade I, 10 cows; Grade II, 12 cows; Grade III, 8 cows). Cows with HL had significantly lower levels of hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total plasma protein (TPP), glucose, total calcium (Ca) and zinc (Zn) and significantly higher levels of plasma urea nitrogen (PUN), non-esterified fatty acid (NEFA), beta hydroxyl butyric acid (BHBA), alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH), TNF–á, CRP, SAA and Hp compared to the negative controls. The APPs, liver ultrasonography, liver enzymes along with NEFA and BHBA could be used as biomarkers for prediction and early diagnosis of HL in cows.

scholarly journals Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

2018 ◽  
Vol 56 (3) ◽  
pp. 436-440 ◽  
Author(s):  
Sigurd Delanghe ◽  
Wim Van Biesen ◽  
Nadeige Van de Velde ◽  
Sunny Eloot ◽  
Anneleen Pletinck ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Acute Phase ◽  
High Performance ◽  
Acute Phase Proteins ◽  
Hemodialysis Patients ◽  
Uremic Toxins ◽  
Bromocresol Green ◽  
Bromocresol Purple ◽  
Acid Glycoprotein ◽  
Reactive Protein

AbstractBackground:Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.Methods:Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.Results:Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.Conclusions:In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.

scholarly journals Saliva: A Miraculous Biofluid for Early Detection of Disease

2013 ◽  
Vol 7 (1) ◽  
pp. 64-68 ◽  
Author(s):  
NS Yadav ◽  
A Singh ◽  
V Saxena ◽  
V Juneja ◽  
V Tiwari ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Sensitivity And Specificity ◽  
Diagnostic Tool ◽  
General Health ◽  
Oral Fluid ◽  
Clinical Applications ◽  
Early Detection Of Disease ◽  
Health And Disease ◽  
Salivary Diagnostics

ABSTRACT Saliva, a multi constituent oral fluid has high potential for the surveillance of general health and disease. To reach the above goal through saliva based diagnostics, two requisites must be fulfilled as discovering biomarker for different diseases among the complicated components of saliva and advancing sensitivity and specificity of biomarkers through persistent development of technologies with collective efforts over several years. Saliva has been demonstrated to be a promising bodily fluid for early diagnosis of disease and salivary diagnostics has exhibited tremendous potential in clinical applications. This review presents an impression of the value of saliva as a credible diagnostic tool, the innovation of salivary biomarkers and development of salivary diagnostics in the future.

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