scholarly journals F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis

2018 ◽  
Vol 116 (1) ◽  
pp. 319-327 ◽  
Author(s):  
Yang Zhang ◽  
Jie Zhang ◽  
Jinliang Guo ◽  
Fanglin Zhou ◽  
Somesh Singh ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Crop Production ◽  
Arable Land ◽  
Acid Soils ◽  
26S Proteasome ◽  
Protein Encoding ◽  
Proteasome Pathway ◽  
Encoding Gene ◽  
The Stability ◽  
F Box Protein

Aluminum (Al) toxicity is a major factor limiting crop production on acid soils, which represent over 30% of the world’s arable land. Some plants have evolved mechanisms to detoxify Al. Arabidopsis, for example, secretes malate via the AtALMT1 transporter to chelate and detoxify Al. The C2H2-type transcription factor STOP1 plays a crucial role in Al resistance by inducing the expression of a set of genes, including AtALMT1. Here, we identify and characterize an F-box protein-encoding gene regulation of Atalmt1 expression 1 (RAE1) that regulates the level of STOP1. Mutation and overexpression of RAE1 increases or decreases the expression of AtALMT1 and other STOP1-regulated genes, respectively. RAE1 interacts with and promotes the degradation of STOP1 via the ubiquitin-26S proteasome pathway, while Al stress promotes the accumulation of STOP1. We find that STOP1 up-regulates RAE1 expression by directly binding to the RAE1 promoter, thus forming a negative feedback loop between STOP1 and RAE1. Our results demonstrate that RAE1 influences Al resistance through the ubiquitination and degradation of STOP1.

The zinc finger transcription factor ATF1 regulates aluminum tolerance in barley

2020 ◽  
Vol 71 (20) ◽  
pp. 6512-6523
Author(s):  
Liyuan Wu ◽  
Yiyi Guo ◽  
Shengguan Cai ◽  
Liuhui Kuang ◽  
Qiufang Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Al Tolerance ◽  
Al Stress ◽  
Transcription Factor 1 ◽  
Differently Expressed Genes

Abstract Aluminum (Al) toxicity is a major abiotic stress that restricts crop production in acid soils. Plants have evolved internal and external mechanisms of tolerance, and among them it is well known that AtSTOP1 and OsART1 are key transcription factors involved in tolerance through regulation of multiple downstream genes. Here, we identified the closest homolog of these two proteins in barley, namely HvATF1, Al-tolerance Transcription Factor 1, and determined its potential function in Al stress. HvATF1 is expressed in the nucleus, and functions in transcriptional activation. The transcription of HvATF1 was found to be constitutive in different tissues, and was little affected by Al stress. Knockdown of HvATF1 by RNAi resulted in increased Al sensitivity. Transcriptomics analysis identified 64 differently expressed genes in the RNAi lines compared to the wild-type, and these were considered as candidate downstream genes regulated by HvATF1. This study provides insights into the different molecular mechanisms of Al tolerance in barley and other plants.

scholarly journals The Arabidopsis F-Box Protein CORONATINE INSENSITIVE1 Is Stabilized by SCFCOI1 and Degraded via the 26S Proteasome Pathway

2013 ◽  
Vol 25 (2) ◽  
pp. 486-498 ◽  
Author(s):  
Jianbin Yan ◽  
Haiou Li ◽  
Shuhua Li ◽  
Ruifeng Yao ◽  
Haiteng Deng ◽  
...  
Keyword(s):  
26S Proteasome ◽  
Proteasome Pathway ◽  
F Box Protein

scholarly journals Problems of sour soil fertility increasing in Tomsk region

2018 ◽  
Vol 48 (3) ◽  
pp. 31-37 ◽  
Author(s):  
I. B. Sorokin ◽  
A. V. Gaag ◽  
Iu. V. Chudinova ◽  
E. A. Sirotina
Keyword(s):  
Agricultural Production ◽  
Crop Production ◽  
Agricultural Land ◽  
Arable Land ◽  
Acid Soils ◽  
Weighted Average ◽  
Fertilizer Efficiency ◽  
Improve Efficiency ◽  
Tomsk Region

The paper reveals the experimental results of long-term agroecological monitoring of agricultural land, conducted by Tomsk state agrochemical service, and experiments on designing the measures to improve efficiency of acidic arable land. In Tomsk region, 83% of arable land is acidic and the degree of acidity increases annually. Every 10 years weighted average pH is reduced on 0.1-0.2 units; the area of acidic soils increases. There is an urgent need for liming these soils in order to improve efficiency of agricultural production in Tomsk region. According to the calculations, the region requires 3,248 million tons of chalky flour. The region has its own large deposits of limestone, for example Kamenskoe (61 million tons), located 50 km from Tomsk, where it is possible to produce valuable meliorant, increasing efficiency of agricultural production. The authors make case, that for successful development of agricultural production in Tomsk region, it is necessary to design and implement long-term target program “Chalking of acid soils in Tomsk region”, which assumes compensation for agricultural producers for the part of the cost of liming; design and technical support of works; local production of limestone meliorants. Implementation of the acidic soil liming program will increase annual crop production in Tomsk region on 54.9 - 129.2 thousand tons of grain units, therefore it will increase annual income on 494.1 - 1162.8 million rubles (in 2017 prices), as well as ensure further increase in agricultural land fertility with high fertilizer efficiency, product quality and profitability.

scholarly journals Fbxw15/Fbxo12J Is an F-Box Protein-Encoding Gene Selectively Expressed in Oocytes of the Mouse Ovary1

2008 ◽  
Vol 78 (4) ◽  
pp. 714-725 ◽  
Author(s):  
Elsa De La Chesnaye ◽  
Bredford Kerr ◽  
Alfonso Paredes ◽  
Horacio Merchant-Larios ◽  
Juan Pablo Méndez ◽  
...  
Keyword(s):  
Protein Encoding ◽  
Encoding Gene ◽  
F Box Protein

scholarly journals Mechanisms of adaptation of small grains to soil acidity

2010 ◽  
pp. 107-120 ◽  
Author(s):  
Ivica Djalovic ◽  
Ivana Maksimovic ◽  
Rudolf Kastori ◽  
Miodrag Jelic
Keyword(s):  
Crop Production ◽  
Soil Acidity ◽  
Arable Land ◽  
Acid Soils ◽  
Mineral Elements ◽  
Al Toxicity ◽  
Mineral Nutrient ◽  
Soil Conditions ◽  
Small Grains ◽  
Mineral Soils

Acid soils limit crop production on 30-40% of the world's arable land and up to 70% of the world's potentially arable land. Over 60% of the total arable lands in Serbia are acid soils. Soil acidity is determined by hydrogen (H+) in soil solution and it is influenced by edaphic, climatic, and biological factors. Major constraints for plant growth on acid mineral soils are toxic concentrations of mineral elements like Al of H+ and/or low mineral nutrient availability due to low solubility (e.g. P and Mo) or low reserves and impaired uptake (e.g. Mg2+) at high H+ concentrations. Aluminum (Al) toxicity is primary factor limiting crop production on acid soils. This review examines our current understanding of mechanisms of Al-toxicity, as well as the physiological and genetic basis for Al-toxicity and tolerance. Inhibition of root growth by Al leads to more shallow root systems, which may affect the capacity for mineral nutrient acquisition and increase the risk of drought stress. Of the two principal strategies (tolerance and avoidance) of plants for adaptation to adverse soil conditions, the strategy of avoidance is more common for adaptation to acid mineral soils. At the same, the short view of the most important genetics tolerance mechanisms, developed and determined in some small grains genotypes, is showed as well.

scholarly journals Characterization of Arabidopsis thaliana R2R3 S23 MYB Transcription Factors as Novel Targets of the Ubiquitin Proteasome-Pathway and Regulators of Salt Stress and Abscisic Acid Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Chase Beathard ◽  
Sutton Mooney ◽  
Raed Al-Saharin ◽  
Aymeric Goyer ◽  
Hanjo Hellmann
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Expression Patterns ◽  
26S Proteasome ◽  
E3 Ligases ◽  
Ubiquitin Proteasome Pathway ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway

Rapid response to environmental changes and abiotic stress to coordinate developmental programs is critical for plants. To accomplish this, plants use the ubiquitin proteasome pathway as a flexible and efficient mechanism to control protein stability and to direct cellular reactions. Here, we show that all three members of the R2R3 S23 MYB transcription factor subfamily, MYB1, MYB25, and MYB109, are degraded by the 26S proteasome, likely facilitated by a CUL3-based E3 ligase that uses MATH-BTB/POZ proteins as substrate adaptors. A detailed description of MYB1, MYB25, and MYB109 expression shows their nuclear localization and specific tissue specific expression patterns. It further demonstrates that elevated expression of MYB25 reduces sensitivities toward abscisic acid, osmotic and salt stress in Arabidopsis, while downregulation of all S23 members results in hypersensitivities. Transcriptional profiling in root and shoot of seedlings overexpressing MYB25 shows that the transcription factor widely affects cellular stress pathways related to biotic and abiotic stress control. Overall, the work extends our knowledge on proteins targeted by CUL3-based E3 ligases that use MATH-BTB/POZ proteins as substrate adaptors and provides first information on all members of the MYB S23 subfamily.

scholarly journals DEPTOR ubiquitination and destruction by SCFβ-TrCP

2012 ◽  
Vol 303 (2) ◽  
pp. E163-E169 ◽  
Author(s):  
Zhiwei Wang ◽  
Jiateng Zhong ◽  
Daming Gao ◽  
Hiroyuki Inuzuka ◽  
Pengda Liu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Critical Role ◽  
Mammalian Target Of Rapamycin ◽  
26S Proteasome ◽  
Dependent Manner ◽  
The Novel ◽  
Functional Aspects ◽  
Ubiquitin Ligase Complex ◽  
The Stability ◽  
F Box Protein

β-Transducin repeats-containing protein (β-TrCP) is the substrate recognition subunit of the SCF (SKP1, CUL1, and F-box protein)-type E3 ubiquitin ligase complex. SCFβ-TrCP ubiquitinates specifically phosphorylated substrates to promote their subsequent destruction by the 26S proteasome and plays a critical role in various human diseases including tumorigenesis. We and others (Duan S et al. Mol Cell 44: 317–324, 2011; Gao D et al. Mol Cell 44: 290–303, 2011; Zhao Y et al. Mol Cell 44: 304–316, 2011) recently reported that SCFβ-TrCP regulates cell growth and autophagy by controlling the ubiquitination and destruction of DEPTOR, an endogenous mammalian target of rapamycin inhibitor, in a phosphorylation-dependent manner. In this review, we discuss β-TrCP's new downstream substrate, DEPTOR, as well as summarize the novel functional aspects of β-TrCP in controlling cell growth and regulating autophagy, in part through governing the stability of DEPTOR.

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