scholarly journals Nonlinear hydrodynamic instability and turbulence in pulsatile flow

2020 ◽  
Vol 117 (21) ◽  
pp. 11233-11239 ◽  
Author(s):  
Duo Xu ◽  
Atul Varshney ◽  
Xingyu Ma ◽  
Baofang Song ◽  
Michael Riedl ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Layer ◽  
Hydrodynamic Instability ◽  
Finite Amplitude ◽  
Low Flow ◽  
Shear Stresses ◽  
Helical Vortices ◽  
Endothelial Cell Layer ◽  
Flow Deceleration ◽  
Pulsation Cycle

Pulsating flows through tubular geometries are laminar provided that velocities are moderate. This in particular is also believed to apply to cardiovascular flows where inertial forces are typically too low to sustain turbulence. On the other hand, flow instabilities and fluctuating shear stresses are held responsible for a variety of cardiovascular diseases. Here we report a nonlinear instability mechanism for pulsating pipe flow that gives rise to bursts of turbulence at low flow rates. Geometrical distortions of small, yet finite, amplitude are found to excite a state consisting of helical vortices during flow deceleration. The resulting flow pattern grows rapidly in magnitude, breaks down into turbulence, and eventually returns to laminar when the flow accelerates. This scenario causes shear stress fluctuations and flow reversal during each pulsation cycle. Such unsteady conditions can adversely affect blood vessels and have been shown to promote inflammation and dysfunction of the shear stress-sensitive endothelial cell layer.

The Effects of Shear Stress on Endothelial Cells at Hypothermic Temperatures

2002 ◽  
Author(s):  
John H. Slater ◽  
Shailendra Jain ◽  
Robin N. Coger ◽  
Charles Y. Lee
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Low Flow ◽  
Shear Stresses ◽  
Liver Sinusoids ◽  
Endothelial Cell Cultures ◽  
Shear Stress Response

Hypothermic machine perfusion preservation (MPP) has proven to be a successful technique for hypothermic kidney storage, however this technology has not successfully been applied to the liver. Recent research has indicated that the endothelial cells lining the liver sinusoids display rounding phenomena during MPP that is not fully understood. In order to gain a better understanding of endothelial cell shear stress response and the factors that induce rounding, a temperature-controlled micro-shear chamber has been designed and fabricated. The micro-shear chamber has been used to apply shear stresses, corresponding to those imposed during MPP, to rat liver primary endothelial cell cultures in order to form an understanding of how these stresses affect endothelial cell morphology. The chamber allows for the application of shear stresses ranging from 0.2 ± .01 dynes/cm2 to 2.3 ± 0.3 dynes/cm2, corresponding to what occurs during MPP.] Twenty-four hour in vitro experiments with shear stresses ranging from 0 to 1.49 dynes/cm2 at 4 °C were conducted in order to replicate in vivo conditions of the liver during hypothermic MPP. It has been demonstrated that endothelial cell rounding increases with increasing shear and can be prevented by utilizing low flow rates.

scholarly journals Ultralarge von Willebrand Factor Fibers Mediate LuminalStaphylococcus aureusAdhesion to an Intact Endothelial Cell Layer Under Shear Stress

Circulation ◽  
2013 ◽  
Vol 128 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Karin I. Pappelbaum ◽  
Christian Gorzelanny ◽  
Sandra Grässle ◽  
Jan Suckau ◽  
Matthias W. Laschke ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Von Willebrand Factor ◽  
Cell Layer ◽  
Endothelial Cell Layer ◽  
Von Willebrand ◽  
Willebrand Factor

scholarly journals Three-Dimensional Numerical Simulations and Antifouling Mechanism of Microorganisms on Microstructured Surfaces

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 319
Author(s):  
Hongyue Yang ◽  
Songling Wang ◽  
Chunxi Li ◽  
Hengfan Li
Keyword(s):  
Shear Stress ◽  
Flow Velocity ◽  
Vortex Flow ◽  
Flow Direction ◽  
Stress Gradient ◽  
Three Dimensional ◽  
Low Flow ◽  
Shear Stresses ◽  
Wall Area ◽  
Microstructured Surface

As marine biofouling seriously affects the development and utilization of oceans, the antifouling technology of microstructured surface has become a research hotspot due to its green and environmentally friendly advantages. In the present research, the motion models of microorganisms on the surfaces of five rectangular micropits, in co-current and counter-current flow direction, were established. Dynamic mesh technology was used to simulate the movements of microorganisms with different radii in the near-wall area, and the fluid kinematics and shear stress distributions in different-sized micropits were compared. Furthermore, moving microorganisms were included in the three-dimensional microstructure model to achieve the real situation of biofouling. Simulation results revealed that the vortex flow velocity in the micropits increased with the increase of the inlet flow velocity and the existence of the vortex flow effectively reduced the formation of conditioning layers in the micropits. In the downstream and countercurrent directions, the average shear stresses on the wall decreased with the increase of the micropit depth and width, and the shear stress on the inner wall of the Mp1 micropit (a patterned surface arranged with cubes of 2 µm × 2 µm × 2 µm) was found to be the largest. A low shear stress region with a low flow velocity was formed around microorganisms in the process of approaching the microstructured surface. The shear stress gradient of micro-ridge steps increased with the approach of microorganisms, indicating that microridge edges had a better effect on reducing microbial attachment.

Anchoring Fibrils in Arterial Endothelium

1969 ◽  
Vol 27 ◽  
pp. 274-275
Author(s):  
A. Trillo
Keyword(s):  
Carotid Arteries ◽  
Animal Species ◽  
Cell Layer ◽  
Present Communication ◽  
Internal Elastic Lamina ◽  
Endothelial Cell Layer ◽  
Structural Details ◽  
Rats And Mice ◽  
Arterial Endothelium ◽  
Elastic Lamina

There are conflicting reports regarding some fine structural details of arteries from several animal species. Buck denied the existence of a sub-endothelial space, while Karrer and Keech described a space of variable width which separates the endothelium from the underlying internal elastic lamina in aortas of aging rats and mice respectively.The present communication deals with the ultrastrueture of the interface between the endothelial cell layer and the internal elastic lamina as observed in carotid arteries from rabbits of varying ages.

Tire Treadwear — A Comprehensive Evaluation of the Factors: Generic Type, Aspect Ratio, Tread Pattern, and Tread Composition Part IV: Laboratory Measurement of Mechanical Properties and Mechanical Actions of Tires Relating to Treadwear

1986 ◽  
Vol 14 (4) ◽  
pp. 264-291
Author(s):  
K. L. Oblizajek ◽  
A. G. Veith
Keyword(s):  
Mechanical Properties ◽  
Shear Stress ◽  
Contact Zone ◽  
Flexural Rigidity ◽  
Comprehensive Evaluation ◽  
Shear Stiffness ◽  
Shear Stresses ◽  
Lateral Shear ◽  
Band Bending ◽  
Tread Rubber

Abstract Treadwear is explained by specific mechanical properties and actions of tires. Rubber shear stresses in the contact zone between the tire and the road become large at large slip angles. When normal stresses are insufficient to prevent sliding at the rear of the footprint, wear occurs at a rate that depends on test severity. Two experimental approaches are described to relate treadwear to tire characteristics. The first uses transducers imbedded in a simulated road surface to obtain direct measurements of contact stresses on the loaded, freely-rolling, steered tires. The second approach is developed with the aid of a simple carcass, tread-band, tread-rubber tire model. Various tire structural configurations; characterized by carcass spring rate, edgewise flexural band stiffness, and tread rubber shear stiffness; are simulated and lateral shear stress response in the contact zone is determined. Tires featuring high band stiffness and low carcass stiffness generate lower lateral shear stress levels. Furthermore, coupling of tread-rubber stiffness and band flexural rigidity are important in determining level of shear stresses. Laboratory measurements with the described apparatus produced values of tread-band bending and carcass lateral stiffness for several tire constructions. Good correlation is shown between treadwear and a broad range of tire stiffness and test course severities.

Finite Element Analysis of Tire/Rim Interface Forces Under Braking and Cornering Loads

1992 ◽  
Vol 20 (2) ◽  
pp. 83-105 ◽  
Author(s):  
J. P. Jeusette ◽  
M. Theves
Keyword(s):  
Finite Element ◽  
Shear Stress ◽  
Contact Pressure ◽  
Element Model ◽  
Shear Stresses ◽  
Detailed Knowledge ◽  
Stress Distributions ◽  
Element Analysis ◽  
Plane Shear ◽  
Normal Contact

Abstract During vehicle braking and cornering, the tire's footprint region may see high normal contact pressures and in-plane shear stresses. The corresponding resultant forces and moments are transferred to the wheel. The optimal design of the tire bead area and the wheel requires a detailed knowledge of the contact pressure and shear stress distributions at the tire/rim interface. In this study, the forces and moments obtained from the simulation of a vehicle in stationary braking/cornering conditions are applied to a quasi-static braking/cornering tire finite element model. Detailed contact pressure and shear stress distributions at the tire/rim interface are computed for heavy braking and cornering maneuvers.

Determination of the transverse shear stress in layered composites

2020 ◽  
Vol 86 (2) ◽  
pp. 44-53
Author(s):  
Yu. I. Dudarkov ◽  
M. V. Limonin
Keyword(s):  
Shear Stress ◽  
Transverse Shear ◽  
Composite Beam ◽  
Layered Composite ◽  
Equivalent Model ◽  
Shear Stresses ◽  
Transverse Shear Stress ◽  
Layered Composites ◽  
Laminate Composites ◽  
Transverse Shear Stresses

An engineering approach to estimation of the transverse shear stresses in layered composites is developed. The technique is based on the well-known D. I. Zhuravsky equation for shear stresses in an isotropic beam upon transverse bending. In general, application of this equation to a composite beam is incorrect due to the heterogeneity of the composite structure. According to the proposed method, at the first stage of its implementation, a transition to the equivalent model of a homogeneous beam is made, for which the Zhuravsky formula is valid. The transition is carried out by changing the shape of the cross section of the beam, provided that the bending stiffness and generalized elastic modulus remain the same. The calculated shear stresses in the equivalent beam are then converted to the stress values in the original composite beam from the equilibrium condition. The main equations and definitions of the method as well as the analytical equation for estimation of the transverse shear stress in a composite beam are presented. The method is verified by comparing the analytical solution and the results of the numerical solution of the problem by finite element method (FEM). It is shown that laminate stacking sequence has a significant impact both on the character and on the value of the transverse shear stress distribution. The limits of the applicability of the developed technique attributed to the conditions of the validity of the hypothesis of straight normal are considered. It is noted that under this hypothesis the shear stresses do not depend on the layer shear modulus, which explains the absence of this parameter in the obtained equation. The classical theory of laminate composites is based on the similar assumptions, which gives ground to use this equation for an approximate estimation of the transverse shear stresses in in a layered composite package.

scholarly journals Assessing Machine Learning versus a Mathematical Model to Estimate the Transverse Shear Stress Distribution in a Rectangular Channel

Mathematics ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 596
Author(s):  
Babak Lashkar-Ara ◽  
Niloofar Kalantari ◽  
Zohreh Sheikh Khozani ◽  
Amir Mosavi
Keyword(s):  
Shear Stress ◽  
Stress Distribution ◽  
Fuzzy Inference ◽  
Rectangular Channel ◽  
Tsallis Entropy ◽  
Shear Stress Distribution ◽  
Data Series ◽  
Shear Stresses ◽  
Inference System ◽  
Aspect Ratios

One of the most important subjects of hydraulic engineering is the reliable estimation of the transverse distribution in the rectangular channel of bed and wall shear stresses. This study makes use of the Tsallis entropy, genetic programming (GP) and adaptive neuro-fuzzy inference system (ANFIS) methods to assess the shear stress distribution (SSD) in the rectangular channel. To evaluate the results of the Tsallis entropy, GP and ANFIS models, laboratory observations were used in which shear stress was measured using an optimized Preston tube. This is then used to measure the SSD in various aspect ratios in the rectangular channel. To investigate the shear stress percentage, 10 data series with a total of 112 different data for were used. The results of the sensitivity analysis show that the most influential parameter for the SSD in smooth rectangular channel is the dimensionless parameter B/H, Where the transverse coordinate is B, and the flow depth is H. With the parameters (b/B), (B/H) for the bed and (z/H), (B/H) for the wall as inputs, the modeling of the GP was better than the other one. Based on the analysis, it can be concluded that the use of GP and ANFIS algorithms is more effective in estimating shear stress in smooth rectangular channels than the Tsallis entropy-based equations.

scholarly journals Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Semra Zuhal Birol ◽  
Rana Fucucuoglu ◽  
Sertac Cadirci ◽  
Ayca Sayi-Yazgan ◽  
Levent Trabzon
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Vascular System ◽  
Circulatory System ◽  
Disease Process ◽  
Microfluidic Channels ◽  
Shear Stresses ◽  
Stress Effects ◽  
Adhesion Behavior ◽  
Cycling System

AbstractAtherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stress levels, rupture of endothelial cells, accumulation of lipids, and recruitment of leukocytes. Shear stress is one of the main factors that regulates the homeostasis of the circulatory system; therefore, sudden and chronic changes in shear stress may cause severe pathological conditions. In this study, microfluidic channels with cavitations were designed to mimic the shape of the atherosclerotic blood vessel, where the shear stress and pressure difference depend on design of the microchannels. Changes in the inflammatory-related molecules ICAM-1 and IL-8 were investigated in THP-1 cells in response to applied shear stresses in an continuous cycling system through microfluidic channels with periodic cavitations. ICAM-1 mRNA expression and IL-8 release were analyzed by qRT-PCR and ELISA, respectively. Additionally, the adhesion behavior of sheared THP-1 cells to endothelial cells was examined by fluorescence microscopy. The results showed that 15 Pa shear stress significantly increases expression of ICAM-1 gene and IL-8 release in THP-1 cells, whereas it decreases the adhesion between THP-1 cells and endothelial cells.

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