scholarly journals Rumen protein degradation and biosynthesis

1983 ◽  
Vol 50 (3) ◽  
pp. 569-582 ◽  
Author(s):  
L. Raab ◽  
B. Cafantaris ◽  
T. Jilg ◽  
K. H. Menke
Keyword(s):  
Protein Synthesis ◽  
Protein Degradation ◽  
Rumen Fluid ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Total N ◽  
Fluid Medium ◽  
The Difference ◽  
Fermentable Carbohydrates

1. A method is described for the determination of protein degradation based on measurements of ammonia concentration and gas production (Menke et al. 1979) when a feedingstuff was incubated with rumen fluid in vitro.2. NH3 liberated during incubation is in part used for microbial protein synthesis. Production of carbon dioxide and methane can be regarded as a measure of energy available for protein synthesis. The ratio, gas production: incorporation of NH3-nitrogen was estimated by addition of starch to the substrate. The response in gas production was linear in the range 0–200 mg starch, when starch was added to 0–200mg feedingstuff dry matter and 30 ml rumen fluid-medium mixture.3. Linear regression between NH3-N concentration (y, mg) and gas production (x, ml) yielded an intercept (b0) representing that amountof NH3-N which would be released when no fermentable carbohydrates were available and consequently no bacterial protein synthesis took place.4. The difference between this intercept b0 and NH3-N content in the blank (rumen fluid without substrate added) indicated the amount of NH3 liberated from protein and other N-containing compounds of the feedingstuff incubated. In vitro-degradable N (IVDN) was calculated as a proportion of total N by the equation:

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

Variation in the in vitro hydrolytic activity of rumen and faecal inocula

2002 ◽  
Vol 2002 ◽  
pp. 166-166 ◽  
Author(s):  
M. Afdal ◽  
F.L. Mould ◽  
C. Rymer ◽  
E. Owen ◽  
D.I. Givens
Keyword(s):  
Rumen Fluid ◽  
Hydrolytic Activity ◽  
Potential Method ◽  
Gas Production ◽  
Microbial Populations ◽  
Fluid Medium ◽  
Faecal Material ◽  
Evaluation Systems

Considerable efforts have been made regarding the use of faecal material to provide a microbial inoculum for in vitro feed evaluation systems. However total gas production, rate of gas release and the extent of degradation of feeds incubated using faecal inoculum are lower than those incubated in a rumen fluid medium. It has been suggested that this is due to lower microbial activity, a consequence of the different microflora and reduced microbial numbers (e.g. Mauricio, 1999). Microbial populations are dynamic so, as their enzyme activity profiles change rapidly, little information is obtained from examining these. However, their hydrolytic activity as reflected by their ability to degrade specific substrates can be simply measured and provides a potential method with which to assess the quality of inocula with respect to their use in in vitro systems. The data presented here are from a larger study in which the differences between the hydrolytic activity of faecal material and rumen contents as influenced by the time of sampling were assessed in vitro.

scholarly journals Dynamics of protozoa in the rumen of sheep

1982 ◽  
Vol 48 (2) ◽  
pp. 399-415 ◽  
Author(s):  
R. A. Leng
Keyword(s):  
Protein Synthesis ◽  
Sodium Chloride ◽  
Half Life ◽  
Rumen Fluid ◽  
Specific Radioactivity ◽  
Microbial Protein ◽  
Replacement Rate ◽  
Irreversible Loss ◽  
Fluid Medium

1. Protozoa were labelled by incubating 100 ml rumen fluid with [14C]choline for 1 h. The protozoa were concentrated by centrifugation and then washed with rumen fluid. This reduced residual14C in the fluid medium to insignificant amounts whilestill retaining the viability of the labelled protozoa. Washing procedures using formal saline (40 g formaldehyde/1 saline (9 g sodium chloride/1)) and saline were developed to isolate protozoa for estimation of specific radioactivity.2. The protozoal pool in freshly-collected rumen fluid incubated in vitro retained 90%of the radioactivity for up to 6 h following addition of14C-I a belled protozoa produced as indicated previously. The specific radioactivity of protozoa did not change during the incubation period.3. Protozoa labelled with [14C]choline and then stored until they died rapidly lost14C to methane when they were incubated in rumen fluid or were injected into the rumen. Some [14C]choline was salvaged under these conditions by the live protozoa present as they apparently incorporated up to 13% of the label from the dead protozoa. However, protozoal debris from the injected solution could also have been present in the isolated protozoa.4. The in vitro results suggested that the protozoal preparations were viable, and that the incorporated choline did not have a turnover in excess of the turnover of nitrogen (i.e. specific radioactivity remained constant with time in vitro) suggesting that the dilution of specific radioactivity of protozoa following mixing of a14C-labelled dose of protozoa represented the rate of irreversible loss and also replacement of protozoa in the rumen.5.14C-labelled protozoa had a half-life in the rumen which was greater than that of rumen fluid and in six animals the protozoal replacement rate was l-4·l mg N/min.6. Losses of14C from labelled protozoa in the rumen in methane or via abomasal digesta were 65 and 35% respectively.7. The results suggest that protozoal growth may be as high as 32% of the total microbial protein synthesis in the rumen but that 65% of the protozoa die and are degraded in the rumen.

scholarly journals Effects of ammonia concentration and microbial population on in vitro degradation of 14C-labelled dietary proteins

1991 ◽  
Vol 71 (1) ◽  
pp. 125-133
Author(s):  
M. K. Song ◽  
J. J. Kennelly
Keyword(s):  
Protein Degradation ◽  
Microbial Population ◽  
Rumen Fluid ◽  
Protein Solubility ◽  
Ammonia Concentration ◽  
Barley Grain ◽  
Minor Role ◽  
Reductive Methylation ◽  
Ruminal Fluid

Rumen fluid from two nonlactating cows fed barley silage and rolled barley grain based concentrates (75:25 on a dry matter basis) was incubated for 0.5, 1, 2, 3, and 4 h with 14C-labelled soybean meal (SBM), fish meal (FM) and corn gluten meal (CGM) to examine the effects of ammonia concentration and protein solubility on rate and extent of protein degradation by total mixed ruminal microorganisms (TMM) or mixed ruminal bacteria (MB). Proteins were labelled by reductive methylation. Ammonia concentration in control ruminal fluid was 4.0 mg dL−1; graded levels of 1 M (NH4)2SO4 were added to achieve ammonia concentrations of 10, 20, and 30 mg dL−1 ruminal fluid. Soluble protein was extracted with borate-phosphate buffer (pH 7.8). Removal of protozoa reduced the extent of protein degradation. However, relative to bacteria, protozoa appeared to play a minor role in protein degradation. Rate and extent of protein degradation was not influenced by ammonia concentration, but was highly correlated with protein solubility. Therefore, it is concluded that ammonia concentration is not the primary factor regulating proteolytic activity of rumen microorganisms, rather their activities may depend upon the solubility and physico-chemical properties of proteins. Key words: In vitro, ammonia concentration, protein degradation, microbial population

scholarly journals AMMONIATION OF RICE STRAW AND SUPPLEMENTATION OF Paraserianthes falcataria AND Sapindus rarak ON IN VITRO RUMEN FERMENTATION AND METHANE PRODUCTION

2017 ◽  
Vol 41 (4) ◽  
pp. 420
Author(s):  
Anuraga Jayanegara ◽  
Nanang Krisnawan ◽  
Yeni Widyawati ◽  
Asep Sudarman
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Rumen Fluid ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Paraserianthes Falcataria ◽  
Buffer Mixture ◽  
Experimental Treatments

This study aimed to observe the effects of rice straw ammoniation and supplementation of Paraserianthes falcataria and Sapindus rarak on rumen fermentation and methane production in vitro. Rice straw was ammoniated by adding 2% urea. Rice straw, ammoniated rice straw, P. falcataria leaves and S. rarak fruits were oven-dried and finely ground. Experimental treatments were arranged as follow: rice straw (T1), ammoniated rice straw (T2), T2 80% + P. falcataria 20% (T3), T2 60% + P. falcataria 40% (T4), T2 80% + P. falcataria 10% + S. rarak 10% (T5), and T2 60% + P. falcataria 20% + S. rarak 20% (T6). An amount of 1 g sample from each treatment was added with 100 ml rumen fluid and buffer mixture (1:2 v/v), and incubated in a water bath at 39 ºC for 48 h. The incubation was performed in four replicates and each replicate was represented by four incubation bottles. Results showed that urea treatment increased gas production of rice straw at 24 and 48 h, higher ammonia production, higher IVDMD, and lower methane production as compared to the untreated rice straw (P<0.05). Addition of P. falcataria or S. rarak at lower level produced similar ammonia concentration as ammoniated rice straw whereas their addition at higher level decreased ammonia concentration (P<0.05). Paraserianthes falcataria addition to ammoniated rice straw decreased protozoa population (P<0.05) and S. rarak further decreased the fauna population (P<0.05) as well. Inclusion of S. rarak at 20% DM (T6) produced the lowest methane production both at 24 and 48 h after incubation and lowest methane production per unit of DM degraded. It was concluded that ammoniation of rice straw improved its nutritional quality with lower methane production, and its mixture with P. falcataria and S. rarak further increased the quality and lower the production of methane, respectively.        

scholarly journals Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes

2013 ◽  
Vol 37 (5) ◽  
pp. 457-463 ◽  
Author(s):  
Ana Carolina Fluck ◽  
Gilberto Vilmar Kozloski ◽  
Andressa Ana Martins ◽  
Mariana Patricia Mezzomo ◽  
Filipe Zanferari ◽  
...  
Keyword(s):  
Production Rate ◽  
Nutritional Value ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Chemical Components ◽  
Forage Legumes ◽  
In Vitro Fermentation ◽  
Total N ◽  
Tropical Forage

Inclusion of forage legumes in diet may improve tropical ruminant systems productivity and sustainability. However, it is not well stablished which chemical components more impact their nutritional value. The relationship between chemical composition and in vitro fermentation of tropical forage legume was evaluated with the objective of obtaining indicators of their nutritional value. Samples of Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna aterrina, Stylosantis sp. and Canavalia ensiformis, cut from plants at growth age between 47 to 110 days, were analysed. Total gas production showed negative correlation (P<0.05) with total N (R=-0.51), acid detergent fibre (ADF, R=-0.62) and acid detergent lignin (ADL, R=-0.65), and positive correlation with non-fibre carbohydrates (NFC, R=0.70). Gas production rate was negatively related (P<0.05) to NDF (R=-0.73), ADF (R=-0.62) and ADL (R=-0.74). Ammonia concentration in the incubation medium was positively related (P<0.05) to total (R=0.74) and soluble (R=0.56) N, and negatively related to NFC (R=-0.81). The level of bacterial adhesion on residue of incubation was negatively related (P<0.05) to cell wall components, mainly to ADL (R=-0.57). The inclusion of polyehtylene glycol increased both gas volume and gas production rate whereas decreased ammonia concentration (P<0.05). In conclusion, even at low concentrations tannins impact the in vitro fermentation of tropical legumes. However, among the analyzed chemical components, the ADL was the best indicator of the nutritional value of the tropical forage legumes.

scholarly journals Feeding Value Assessment of Substituting Cassava (Manihot esculenta) Residue for Concentrate of Dairy Cows Using an In Vitro Gas Test

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 307
Author(s):  
Yuhui Zheng ◽  
Yanyan Zhao ◽  
Shenglin Xue ◽  
Wei Wang ◽  
Yajing Wang ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Rumen Fluid ◽  
Gas Production ◽  
The Other ◽  
Holstein Cows ◽  
Value Assessment ◽  
Buffer Solution ◽  
Feeding Value ◽  
Cassava Residue

The feeding value of replacing concentrate with cassava (Manihot esculenta) residue in the feed of Holstein cows was confirmed using an in vitro gas test. The treatments consisted of 0% (control, CON), 5%, 10%, 15%, 20%, 25%, and 30% inclusion of cassava residue in fermentation culture medium composed of buffer solution (50 mL) and filtrated rumen fluid (25 mL). The parameters analyzed included the kinetics of gas production and fermentation indexes. Forty-eight hours later, there were no significant differences on in vitro dry matter disappearance (IVDMD), pH, and microbial crude protein (MCP) content among treatments (p > 0.05). However, the “cumulative gas production at 48 h” (GP48), the “asymptotic gas production” (A), and the “maximum gas production rate” (RmaxG) all increased linearly or quadratically (p < 0.01). The GP48 was significantly higher in the 25% treatment compared to the other treatments, except for the 30% (p < 0.01). The A was significantly larger in the 25% treatment compared to the other treatments, except for the 20% and 30% (p < 0.01). The RmaxG was distinctly larger in the 25% treatment compared to other treatments (p < 0.01); moreover, the “time at which RmaxG is reached” (TRmaxG) and the “time at which the maximum rate of substrate degradation is reached” (TRmaxS) were significantly higher in the 25% treatment than the CON, 20%, and 30% treatments (p < 0.01). Additionally, the content of ammonia-N (NH3-N) in all treatments showed linearly and quadratically decreases (p < 0.01), whereas total volatile fatty acid (VFA), iso-butyrate, butyrate, and iso-valerate contents changed quadratically (p = 0.02, p = 0.05, p = 0.01, and p = 0.02, respectively); all of these values peaked in the 25% treatment. In summary, the 25% treatment was associated with more in vitro gas and VFA production, indicating that this cassava residue inclusion level may be used to replace concentrate in the feed of Holstein cows. However, these results need to be verified in vivo.

Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques

2005 ◽  
Vol 123-124 ◽  
pp. 107-118 ◽  
Author(s):  
Gonzalo Hervás ◽  
Pilar Frutos ◽  
F. Javier Giráldez ◽  
Manuel J. Mora ◽  
Begoña Fernández ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Fermentative Activity

Cell-free, protein-synthesizing system of photosynthetic and heterotrophic Rhodospirillum rubrum

1976 ◽  
Vol 22 (2) ◽  
pp. 304-308
Author(s):  
C. T. Chow
Keyword(s):  
Protein Synthesis ◽  
Protease Activity ◽  
Rhodospirillum Rubrum ◽  
Chemical Compounds ◽  
Protein Synthesis Inhibitors ◽  
Free Protein ◽  
Two Systems ◽  
The Difference ◽  
Vitro Protein

An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylfluoride. The difference in protein-synthesizing activity between the photosynthetic and the heterotrophic systems has been tested in regard to the requirement of various chemicals and the response to protein synthesis inhibitors or various chemical compounds. It has been concluded that only minor differences in protein-synthesizing activity exist between these two systems.

PSXIII-8 Effects of different sources of Zn on in vitro ruminal fermentation and digestibility of a lactation diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 466-466
Author(s):  
Angela R Boyer ◽  
Yun Jiang ◽  
Alon Blakeney ◽  
Dennis Nuzback ◽  
Brooke Humphrey ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Random Effect ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Negative Effects ◽  
Minimum Concentration ◽  
High Metal Content ◽  
Different Sources

Abstract Vistore® minerals are hydroxychloride minerals that feature high metal content and improved bioavailability. This study was conducted to compare different sources of zinc (Zn) on in vitro rumen fermentation parameters. Three ruminally-cannulated Jersey heifers were adapted to a lactation diet for two weeks before used as donors. Three sources of Zn were tested at 20 ppm: No supplemental Zn (CON), ZnSO4, Vistore Zn, and another Zn hydroxychloride (Vistore-competitor). The concentration of Zn in this study was selected from a titration study (0 to 40 ppm ZnSO4) to identify the minimum concentration of ZnSo4 affecting rumen fermentation. The lactation diet (TMR) was dried and ground to 1mm and used as substrate. Rumen fluid was collected two hours after feeding. Substrate (0.5 g) was inoculated with 100 mL of 3:1 McDougall’s buffer: ruminal flued mixture at 39ºC for 24 h. Each treatment was run in triplicate and in three runs. Data were analyzed with R 3.0. The model included fixed effect of treatment and random effect of run. ZnSO4 reduced (P &lt; 0.05) maximum gas production, DMD (54 vs. 55.9%) and cellulose (27.5 and 40.7%) digestibility. acetate to propionate ration (2.20 vs. 2.24) and NH3-N concentration (6.0 vs. 7.0 mg/dL), increased (P &lt; 0.05) propionate % (27.2 vs 26.7%) compared to control. Vistore had higher pH than control (6.44 vs. 6.40, P = 0.02) but did not affect other parameters compared to CON. Vistore-competitor reduced total VFA production compared to control, ZnSO4, and Vistore (94 vs. 102, 106 and 107 mM, respectively, P = 0.01) but did not affect other parameters. In general, Vistore Zn maintained in vitro ruminal fermentation and digestibility, while ZnSO4 had negative effects on both fermentation and digestibility and Vistore-competitor reduced total VFAs. Results indicate hydroxychloride minerals may stabilize rumen parameters versus sulfate sources but different hydroxychloride sources appear to influence rumen parameters differently.

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