Histochemical and histological evaluations of the effects of high incubation temperature on embryonic development of thymus and bursa of Fabricius in broiler chickens

2010 ◽  
Vol 51 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Y. Oznurlu ◽  
I. Celik ◽  
T. Telatar ◽  
E. Sur
Keyword(s):  
Embryonic Development ◽  
Broiler Chickens ◽  
Incubation Temperature ◽  
Bursa Of Fabricius

scholarly journals Effect of cold and warm white light on selected endocrine and immune parameters of broiler embryos and hatchlings

2020 ◽  
Vol 65 (No. 11) ◽  
pp. 431-441
Author(s):  
Angelika Drozdova ◽  
Zuzana Kankova ◽  
Michal Zeman
Keyword(s):  
Embryonic Development ◽  
Thyroid Hormones ◽  
White Light ◽  
Broiler Chickens ◽  
Production Efficiency ◽  
Bursa Of Fabricius ◽  
Corticosterone Concentration ◽  
Pineal Melatonin ◽  
Immune Parameters ◽  
Warm White Light

Lighting conditions during incubation can influence embryonic development, post-hatching ontogeny and production efficiency. Previous studies revealed that different light colours differently affect pineal melatonin biosynthesis in embryos and postembryonic development of broiler chickens, but physiological mechanisms mediating these effects are not known. Cold and warm white light consists of different wavelengths and therefore the aim of the present study was to explore if these two lights can differently influence the development of circadian melatonin biosynthesis, production of thyroid hormones and corticosterone, concentration of metabolites (glucose, cholesterol and triacylglycerols) as well as expression of two important immune genes, presenilin 1 and avian beta-defensin 1 (AvBD-1). We evaluated these traits in embryos before hatching (456, 460, 465, 468 and 472 h of incubation) and in hatchlings. The rhythmic profile of pineal melatonin with higher concentrations during the dark time was determined in both treatment groups. Melatonin levels increased considerably in hatchlings in comparison with embryos, but we found no difference in rhythm characteristics between groups. We did not identify any daily rhythms in plasma corticosterone and thyroid hormone levels in either studied age and no differences were found between light treatments in concentrations of thyroid hormones, corticosterone, metabolites and expression of presenilin and AvBD-1. The expected developmental increase of thyroid hormones was proved. Gene expression of presenilin increased in the duodenum of hatchlings in comparison with embryos, but the expression did not change in the bursa of Fabricius. On the other hand, expression of AvBD-1 decreased in hatchlings compared to embryos in both tissues. Based on these results, we can conclude that the colour temperature of white light did not influence endocrine and immune parameters determined in this study and probably monochromatic rather than polychromatic light should be used to influence embryonic development and postembryonic ontogeny of broiler chickens.

scholarly journals Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides)

2012 ◽  
Vol 70 (2) ◽  
pp. 342-351 ◽  
Author(s):  
Rosario Domínguez-Petit ◽  
Patrick Ouellet ◽  
Yvan Lambert
Keyword(s):  
Embryonic Development ◽  
Reproductive Strategy ◽  
Biochemical Composition ◽  
Incubation Temperature ◽  
Life Stage ◽  
Early Life Stage ◽  
Greenland Halibut ◽  
Hatching Time ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides

Abstract Domínguez-Petit, R., Ouellet, P., and Lambert, Y. 2013. Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides). – ICES Journal of Marine Science, 70: 342–351. Despite the commercial importance of Greenland halibut (GH), important gaps exist in our knowledge of the reproductive and early life stage biology for this species. The present study examined through laboratory experiments the spawning strategy, realized fecundity, egg characteristics, biochemical composition, and embryonic development of GH. The results confirmed the hypothesis that GH is a single-batch spawner producing large eggs, resulting in low realized fecundity. Embryonic development and hatching time are highly dependent on incubation temperature; 50% hatching occurred after 46, 30, and 24 days at 2, 4, and 6°C, respectively. Few changes in the biochemical composition of the eggs are observed during embryonic development. Newly hatched larvae are not well developed, having a large yolk sac, no pigmentation and incomplete development of the jaws. Egg specific density confirmed the mesopelagic distribution of the eggs at sea. However, important buoyancy changes occurring in the last 3–4 days before hatching indicate that larvae hatch higher in the water column. These results are important for understanding advection and dispersion processes of GH eggs and larvae and the connectivity between spawning grounds and nursery areas.

scholarly journals Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler

2019 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Aan Awaludin ◽  
Yudhi Ratna Nugraheni ◽  
Theo Mahiseta Syahniar ◽  
Dyah Laksito Rukmi ◽  
Agus Hadi Prayitno ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Care Management ◽  
Clinical Symptoms ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Farms ◽  
The One ◽  
Organ Changes

Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.

scholarly journals Cryptosporidium sp. infection in the broiler chickens and turkeys on farms in north central Algeria

2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Sihem Guechtouli ◽  
Nora Mimoune ◽  
Chafik-Reda Messai ◽  
Omar Salhi ◽  
Rachid Kaidi ◽  
...  
Keyword(s):  
High Frequency ◽  
Broiler Chickens ◽  
Climatic Conditions ◽  
Bursa Of Fabricius ◽  
Tissue Samples ◽  
North Central ◽  
Genetic Potential ◽  
Hygienic Measures ◽  
The Impact ◽  
Poor Management

This study aimed to determine the prevalence of cryptosporidiosis in broiler chickens and turkeys and the factors that can influence its development, the precision of the most frequent sites of Cryptosporidium, and the severity of lesions associated with the disease. From October 2019 to September 2020, the prevalence of Cryptosporidia infection was determined on 22 farms located in north central Algeria. For each farm, weekly visits were conducted during the study period and information on the type of farming and health status was collected. At these farms, 98 chickens and 22 turkeys were examined and clinical lesions recorded. Analyses of faeces, tissue samples and scrapings made from the intestine, bursa of Fabricius, trachea, cloaca and proventriculus revealed a prevalence of 55% at chicken farms. The age of chickens was found to be a significant factor, with a high frequency of the disease observed in chickens aged between 10 to 50 days with a maximum between 31 and 40 days (84.62%). The parasite was not detected in chickens younger than 7 days. The most common site of Cryptosporidium sp. was the bursa (24.2%), followed by the trachea (21.5%), cloaca (18.9%), intestine (16.3%), and proventriculus (6.6%). In turkeys, the overall prevalence was 41%. The bursa was the most infected organ (23.81%), followed by the cloaca (15.79%) and intestine (9%). For both avian species, data confirmed the impact of season, strain (genetic potential), and rearing conditions on the prevalence of Cryptosporidium sp. To conclude, our results showed that Cryptosporidium was very common on these farms, and was related mainly to climatic conditions and poor management, particularly hygienic measures.

scholarly journals The influence of difference temperature against the rate of embryonic development and larval abnormality of Cantik Grouper (Epinephelus sp.)

2019 ◽  
Vol 2 (1) ◽  
pp. 16
Author(s):  
Aprisianus Julkarman Simbolon ◽  
Ganjar Adhywirawan Sutarjo ◽  
Hariyadi Hariyadi
Keyword(s):  
Low Temperature ◽  
Embryonic Development ◽  
Incubation Temperature ◽  
Temperature Treatment ◽  
Optimum Temperature ◽  
Long Time ◽  
Epinephelus Fuscoguttatus ◽  
Low Levels ◽  
Difference Temperature ◽  
F 31

Cantikgrouper is the hybridization results grouper or cross-breeding between Epinephelus fuscoguttatus as a female and Epinephelus microdon as a male. The main barriers faced in the development of this commodity is still low levels of spawning up to seeding grouper. Based on the background, this study aimed to investigate optimum temperature observations against the rate of embryonic development Epinephelus sp.larvae. This study used the results of artificial spawning eggs.The fertilized eggs were incubated on six pieces of the container temperature treatment;each treatment there was repeated three times.The incubation temperature was kept on (A) 21-22°C; (B) 23-24°C; (C) 25-26°C; (D) 27-28°C; (E) 29-30°C; (F) 31-32°C. Results showed that eggswere incubated at a temperature of 21-22 ℃ embryonic development to a halt in the blastula, and temperature 23-24°C stalled on phasemyomere embryos. The low-temperature incubation period lasts a long time. Temperature 25-26°C needed 18 hours 6 minutes by 8.33% abnormality rate. Temperature 27-28°C needed 16 hours to hatch witha degree of abnormality of 7.6%. Temperature 29-30°C needed 15 hours 1 minute for the hatch tothe degree of abnormality of 5.33%. The 31-32°C temperature needed 14 hours 6 minutes to hatch witha degree of abnormality of 17.3%. The limits of tolerance for the incubation of the eggs ofcantik grouper (Epinephelusspp.) were 26-32°C.The best temperature of each treatment were obtained at a temperature of 29-30°C. Based on our results, it concluded that the changing temperature affected how long eggs could hatch.

scholarly journals Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers

2021 ◽  
Vol 14 (2) ◽  
pp. 523-536
Author(s):  
Essam S. Soliman ◽  
Rania T. Hamad ◽  
Mona S. Abdallah
Keyword(s):  
Bacillus Subtilis ◽  
Broiler Chickens ◽  
Trichophyton Mentagrophytes ◽  
Antimicrobial Activities ◽  
Lymphoid Hyperplasia ◽  
Bursa Of Fabricius ◽  
Tissue Architecture ◽  
E Coli

Background and Aim: Probiotics improve intestinal balance through bacterial antagonism and competitive exclusion. This study aimed to investigate the in vitro antimicrobial activity, as well as the in vivo preventive, immunological, productive, and histopathological modifications produced by probiotic Bacillus subtilis. Materials and Methods: The in vitro antimicrobial activities of B. subtilis (5×106 CFU/g; 0.5, 1.0*, 1.5, and 2.0 g/L) were tested against Escherichia coli O157: H7, Salmonella Typhimurium, Candida albicans, and Trichophyton mentagrophytes after exposure times of 0.25, 0.5, 1, and 2 h using minimal inhibitory concentration procedures. A total of 320 1-day-old female Ross broiler chickens were divided into five groups. Four out of the five groups were supplemented with 0.5, 1.0*, 1.5, and 2.0 g/L probiotic B. subtilis from the age of 1 day old. Supplemented 14-day-old broiler chickens were challenged with only E. coli O157: H7 (4.5×1012 CFU/mL) and S. Typhimurium (1.2×107 CFU/mL). A total of 2461 samples (256 microbial-probiotic mixtures, 315 sera, 315 duodenal swabs, and 1575 organs) were collected. Results: The in vitro results revealed highly significant (p<0.001) killing rates at all-time points in 2.0 g/L B. subtilis: 99.9%, 90.0%, 95.6%, and 98.8% against E. coli, S. Typhimurium, C. albicans, and T. mentagrophytes, respectively. Broilers supplemented with 1.5 and 2.0 g/L B. subtilis revealed highly significant increases (p<0.01) in body weights, weight gains, carcass weights, edible organs' weights, immune organs' weights, biochemical profile, and immunoglobulin concentrations, as well as highly significant declines (p<0.01) in total bacterial, Enterobacteriaceae, and Salmonella counts. Histopathological photomicrographs revealed pronounced improvements and near-normal pictures of the livers and hearts of broilers with lymphoid hyperplasia in the bursa of Fabricius, thymus, and spleen after supplementation with 2.0 g/L B. subtilis. Conclusion: The studies revealed that 1.5-2.0 g of probiotic B. subtilis at a concentration of 5×106 CFU/g/L water was able to improve performance, enhance immunity, and tissue architecture, and produce direct antimicrobial actions.

Thermal influence on the embryonic development and hatching rate of the flameback pygmy angelfish Centropyge aurantonotus eggs

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 80-82
Author(s):  
Raoani Cruz Mendonça ◽  
Sarah Pittigliani Ikebata ◽  
Sérgio Leandro Araújo-Silva ◽  
João Vitor Azevedo Manhães ◽  
Mônica Yumi Tsuzuki
Keyword(s):  
Embryonic Development ◽  
Incubation Temperature ◽  
Abiotic Factors ◽  
Environmental Parameters ◽  
Hatching Rate ◽  
Influence Of Temperature ◽  
Different Temperatures ◽  
Influence Of Temperature On ◽  
Thermal Influence ◽  
Hatching Rates

SummaryThe flameback pygmy angelfish Centropyge aurantonotus, highly appreciated and valued by the aquarium market, is heavily harvested and traded. Temperature is one of the abiotic factors that has the most influence on fish development, especially in the early stages of life. For captive production, it is essential to know the appropriate environmental parameters for each species. In this sense, this study aimed to evaluate the influence of temperature on the embryonic development and hatching rates of C. aurantonotus incubated at six different temperatures (20, 22, 24, 26, 28, 30°C). Embryonic development events were very similar in terms of morphological and chronological characteristics compared with other species of the genus Centropyge. Incubation time was inversely proportional to temperature. The treatment at 22°C required twice the time of that required by 30°C treatment for hatching to occur. The best incubation temperature range was 24–28°C. Values below 22°C and at 30°C showed lower hatching rates compared with other treatments. Based on these results, the recommended temperature at which to incubate C. aurantonotus eggs is between 24–28°C.

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