Prescient diagnostic analysis: A proposed new approach to evaluating plant nutrient diagnostic methods

Актуальность. Миодистрофия Ландузи-Дежерина (МЛД) является одной из наиболее часто встречающихся мышечных дистрофий. В 95% случаев заболевание связано с частичной делецией массива повторов D4Z4 на одном из аллелей 4-й хромосомы. Существующие диагностические методики гибридизации по Саузерну и молекулярного комбинга являются ресурсо- и времязатратными. В настоящее время в Российской Федерации молекулярно-генетическая диагностика МЛД не проводится. Цель. Поиск новых подходов к диагностике МЛД для использования в молекулярно-генетических лабораториях. Методы. ДНК выделялась в агарозных блоках и подвергалась обработке эндонуклеазой EcoRI. Полученные фрагменты ДНК разделялись методом пульс-электрофореза в агарозном геле, после этого агарозный гель фрагментировался согласно маркеру молекулярного веса и использовался в качестве матрицы для полимеразной цепной реакции (ПЦР). Принадлежность полученных ПЦР-продуктов к последовательностям повторов D4Z4 4-й хромосомы подтверждалась секвенированием по Сэнгеру. Результаты. Протокол пульс-электрофореза оптимизирован таким образом, что после всех этапов ДНК в агарозном геле пригодна для использования в качестве матрицы для ПЦР. Разработана ПЦР-система специфичной амплификации контрольных ДНК-матриц 4-й хромосомы и подтверждена секвенированием принадлежность получаемых ПЦР-продуктов к последовательности повторов D4Z4 4-й хромосомы. Выводы. Показана возможность использования ДНК в агарозном геле после пульс-электрофореза в качестве матрицы для детекции повторов D4Z4 методом ПЦР. Представленная ПЦР-система специфично амплифицирует последовательности D4Z4 4-й хромосомы. Используя данную ПЦР-систему и геномную ДНК пациента с известной длиной массива повторов D4Z4 проведена успешная диагностика МЛД. Таким образом разработан новый подход к диагностике МЛД для использования в молекулярно-генетических лабораториях. Relevance. Facioscapulohumeral muscular dystrophy (FSHD) is one of the most common muscular dystrophies. In 95% of cases, the disease is associated with partial deletion of the array of the D4Z4 repeats on one of the alleles of the 4th chromosome. The existing diagnostic methods of Southern blotting and molecular combing are quite resource-and time-consuming. At the moment, molecular genetic diagnostic of FSHD is not provided on the territory of the Russian Federation. Aim: to find new approaches for molecular genetic diagnostic of FSHD acceptable for use in standard molecular genetic laboratories Materials and methods: DNA isolated in agarose plugs and treated by the EcoRI restriction enzyme. DNA fragments then were separated by pulse field gel electrophoresis (PFGE) in agarose gel. After PFGE, the agarose gel was fragmented and used as a matrix for PCR. The identity of the obtained PCR products to the sequence of the D4Z4 repeats of the 4th chromosome was confirmed by sequencing by Sanger. Results. The PFGE protocol is optimized in such a way that, after all stages, DNA in agarose gel is suitable for use as a matrix for PCR. We achieve a specific amplification of the control DNA matrices of the 4th chromosome and confirm belonging of the PCR products to the sequence of D4Z4 repeats of the 4th chromosome by the Senger sequencing. Conclusions. This paper shows the possibility of using DNA in agarose gel after PFGE as a matrix for detection of D4Z4 repeats by PCR. The presented PCR system specifically amplify sequence of the 4th chromosome D4Z4 repeats. Using this PCR system and genomic DNA of a patient with a known length of the D4Z4 repeats array, a successful diagnosis of FSHD was performed. Thus, we propose a new approach for FSHD diagnostic, acceptable for use in standard molecular genetic laboratories.

Download Full-text

To the Evaluation of the Insulation State Based on the Analysis of Partial Discharges

Journal of Physics Conference Series ◽

10.1088/1742-6596/2096/1/012192 ◽

2021 ◽

Vol 2096 (1) ◽

pp. 012192

Author(s):

S Yu Tetiora ◽

N V Silin ◽

N N Petrunko

Keyword(s):

Deterministic Model ◽

Partial Discharge ◽

Electrical Equipment ◽

Diagnostic Value ◽

Point Of View ◽

Partial Discharges ◽

Diagnostic Methods ◽

Application Area ◽

New Approach ◽

Discharge Modeling

Abstract The existing partial discharge models, their diagnostic value, and application area are analyzed in the article. The models are considered from the point of view of their improvement or the possibility of creating new diagnostic methods for electrical equipment based on the characteristics of partial discharges. As an example of the implementation of a new approach to partial discharge modeling, a quasi-deterministic model is considered, which makes it possible to obtain information on the real number of cavities in the insulation of high-voltage equipment.

Download Full-text

DIAGNOSTICS OF CRISIS CONDITIONS OF THE AVIATION ENTERPRISE

Three Seas Economic Journal ◽

10.30525/2661-5150/2020-2-7 ◽

2020 ◽

Vol 1 (2) ◽

pp. 42-47

Author(s):

Larysa Turova ◽

Tetiana Gavrilko

Keyword(s):

Management Accounting ◽

Diagnostic Methods ◽

Systemic Approach ◽

Management Systems ◽

Aviation Industry ◽

Diagnostic Analysis ◽

Accounting Systems ◽

Financial Condition ◽

Management Accounting Systems ◽

Crisis Conditions

The purpose of the article is to study the systems and methods of diagnostics of the crisis conditions of the aviation enterprise, to justify the role of controlling as a management accounting system in the detection and prevention of crisis phenomena, to clarify the mechanism and iterations of diagnostics of the crisis conditions taking into account the use of appropriate management systems. Methodology. The key provisions of economic and financial theory, methodological and methodological principles of crisis diagnosis of enterprises, presented in the works of Ukrainian and foreign scientists, were used as the theoretical basis of the study. The application of a systemic approach allowed to analyze the essence of diagnostics of crisis conditions, its purpose and objectives. The methods of logical generalization used in the research process allowed to formulate the conclusions on the conditions for the effective implementation of diagnostics of crisis conditions based on improving of the functioning of management accounting systems and management systems. Results. The study allowed us to analyze the factors that causing crisis conditions in the aviation industry. The main methods for diagnosing crisis conditions are analyzed placing emphasis on the special place that belongs to management accounting and the use of management systems when diagnosing crisis conditions. The basic provisions of effective organization of management accounting at aviation enterprises to form the information base at development of the strategy for crisis prevention or recovering from crisis conditions are defined. The peculiarities of diagnostic analysis of the financial condition of the enterprise as a system of diagnostic methods are identified; on the basis of a systemic approach, they allow to establish causal changes in the economic system through quantitative and qualitative measurement of the influence of factors on adjustments and development. Features of different types of diagnostic analysis depending on the hierarchy in the management system, goals and directions of analysis, as well as the availability of information about the problem areas of the enterprise, are discussed. Tasks that need to be implemented to achieve the goal of diagnostic analysis are specified. The necessity of diagnostics of possible crisis conditions of the aviation enterprise with simultaneous risk research and its influence on financial stability of the investigated object is justified. The system of indicator values for diagnostics of a financial condition of the aviation enterprise is offered. The sequence of calculations for the implementation of monitoring and controlling process of the financial condition of aviation enterprises is presented. Practical implications. The approach for diagnosing financial condition of the enterprise, which allows to identify the symptoms and threats of the financial crisis and to develop the policy for prevention (anticipation) of weak signals of a crisis situation based on the analysis, is justified. The conditions of effective application of management accounting systems on the basis of monitoring and controlling and modern methods of diagnostics of the financial condition of aviation enterprises are determined. The directions for improvement of analytical activity on the basis of information systems and technologies for the aviation industry are justified. Value/originality. Results given in the article can be used for further research on the problem of diagnosing the crisis conditions of aviation enterprises.

Download Full-text

Pharmaceutical Inventions

Patents for Chemicals, Pharmaceuticals, and Biotechnology ◽

10.1093/oso/9780199684731.003.0016 ◽

2016 ◽

Author(s):

Philip W. Grubb ◽

Peter R. Thomsen ◽

Tom Hoxie ◽

Gordon Wright

Keyword(s):

Trips Agreement ◽

Diagnostic Methods ◽

European Patent ◽

New Approach ◽

Animal Body ◽

European Patent Convention ◽

New Chemical Entities ◽

Per Se ◽

The Law ◽

The Uk

This chapter considers the law governing the patentability of pharmaceutical inventions, including new chemical entities, pharmaceutical compositions, and dosage regimes. Provisions in the UK Patents Act 1977 and the European Patent Convention (EPC) exclude from patentability ‘methods for treatment of the human or animal body by surgery or therapy and diagnostic methods practised on the human or animal body’, although this does not prevent a substance or composition from being patentable for use in any of these methods. Under the EPC, such methods were excluded by being defined as not industrially applicable; the new approach introduced by EPC 2000 is more logical and also clarifies the patentability of further medical uses. Novel compounds that have a pharmaceutical utility are patentable per se in all countries that have implemented the TRIPs Agreement.

Download Full-text

Prenatal prediction and typing of placental invasion using MRI deep and radiomic features

BioMedical Engineering OnLine ◽

10.1186/s12938-021-00893-5 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Rongrong Xuan ◽

Tao Li ◽

Yutao Wang ◽

Jian Xu ◽

Wei Jin

Keyword(s):

Placental Tissue ◽

Objective Evaluation ◽

Regions Of Interest ◽

Diagnostic Methods ◽

Multi Layer Perceptron ◽

Mr Image ◽

New Approach ◽

Average Accuracy ◽

Therapeutic Measures ◽

Placental Invasion

Abstract Background To predict placental invasion (PI) and determine the subtype according to the degree of implantation, and to help physicians develop appropriate therapeutic measures, a prenatal prediction and typing of placental invasion method using MRI deep and radiomic features were proposed. Methods The placental tissue of abdominal magnetic resonance (MR) image was segmented to form the regions of interest (ROI) using U-net. The radiomic features were subsequently extracted from ROI. Simultaneously, a deep dynamic convolution neural network (DDCNN) with codec structure was established, which was trained by an autoencoder model to extract the deep features from ROI. Finally, combining the radiomic features and deep features, a classifier based on the multi-layer perceptron model was designed. The classifier was trained to predict prenatal placental invasion as well as determine the invasion subtype. Results The experimental results show that the average accuracy, sensitivity, and specificity of the proposed method are 0.877, 0.857, and 0.954 respectively, and the area under the ROC curve (AUC) is 0.904, which outperforms the traditional radiomic based auxiliary diagnostic methods. Conclusions This work not only labeled the placental tissue of MR image in pregnant women automatically but also realized the objective evaluation of placental invasion, thus providing a new approach for the prenatal diagnosis of placental invasion.

Download Full-text

A New Approach to the Diagnosis of Enzootic Leukosis by Genetic Markers of Bovine Leukemia Virus

Biointerface Research in Applied Chemistry ◽

10.33263/briac124.44484462 ◽

2021 ◽

Vol 12 (4) ◽

pp. 4448-4462

Keyword(s):

Genetic Markers ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

High Sensitivity ◽

Bioinformatic Analysis ◽

Diagnostic Methods ◽

Online Resources ◽

Full Genome ◽

New Approach ◽

Pcr Method

In this paper, we consider the gp51 and p24 genes of the bovine leukemia virus (BLV) as "DNA-targets" for the detection of the BLV proviral DNA. We found that the gp51 and p24 genes of BLV are sufficiently specific to be used as marker sequences in PCR. The bioinformatic analysis of intraspecies specificity was conducted for each primer combination, and as a result, we found that they are complementary to most of the full-genome nucleotide sequences of BLV isolates displayed at NCBI online resources. We were able to show that the designed primer combinations possess high sensitivity, reaching 15 cells/μl in both variants. Furthermore, we compared the PCR method with the suggested primer combinations against other laboratory diagnostic methods, namely BLV-AGID and ELISA. Regarding BLV detection, the PCR method with each primer combination applied to genes gp51 and p24 separately was more effective than both AGID and ELISA; it surpassed the former by 13.5% and 14.4%, respectively, and the latter by 6.1% and 7.0%, respectively. The simultaneous use of both primer combinations on a single amplified mixture showed the largest number of BLV-infected cows (53 animals) compared to the separate amplification of each genetic marker.

Download Full-text

The O–C diagrams of minor planets − a new approach to modelling the rotation

International Astronomical Union Colloquium ◽

10.1017/s0252921100031407 ◽

1999 ◽

Vol 173 ◽

pp. 185-188

Author(s):

Gy. Szabó ◽

K. Sárneczky ◽

L.L. Kiss

Keyword(s):

Error Analysis ◽

Time Dependence ◽

Theoretical Work ◽

Minor Planet ◽

Minor Planets ◽

New Approach ◽

Preliminary Results ◽

Ccd Observations

AbstractA widely used tool in studying quasi-monoperiodic processes is the O–C diagram. This paper deals with the application of this diagram in minor planet studies. The main difference between our approach and the classical O–C diagram is that we transform the epoch (=time) dependence into the geocentric longitude domain. We outline a rotation modelling using this modified O–C and illustrate the abilities with detailed error analysis. The primary assumption, that the monotonity and the shape of this diagram is (almost) independent of the geometry of the asteroids is discussed and tested. The monotonity enables an unambiguous distinction between the prograde and retrograde rotation, thus the four-fold (or in some cases the two-fold) ambiguities can be avoided. This turned out to be the main advantage of the O–C examination. As an extension to the theoretical work, we present some preliminary results on 1727 Mette based on new CCD observations.

Download Full-text

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073933 ◽

1973 ◽

Vol 31 ◽

pp. 698-699

Author(s):

V. Mizuhira ◽

Y. Futaesaku

Keyword(s):

Cross Section ◽

Tannic Acid ◽

Elastic Fiber ◽

The Other ◽

New Approach ◽

Tissue Block ◽

Active Reaction ◽

The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

Download Full-text

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120266 ◽

1985 ◽

Vol 43 ◽

pp. 714-715

Author(s):

K. Chien ◽

R. Van de Velde ◽

I.P. Shintaku ◽

A.F. Sassoon

Keyword(s):

Cell Monolayer ◽

Cell Types ◽

Surface Antigens ◽

Immunoperoxidase Method ◽

Reaction Step ◽

Hot Plate ◽

Air Drying ◽

New Approach ◽

Cell Monolayers ◽

Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Download Full-text