A soft tactile sensor featuring subcutaneous tissue structure with collagen fibers

2020 ◽  
pp. 1-12
Author(s):  
Y. Sonoi ◽  
Y. Tanaka ◽  
J. Nishizawa ◽  
N. Usuda
Keyword(s):  
Subcutaneous Tissue ◽  
Tactile Sensor ◽  
Collagen Fibers ◽  
Tissue Structure

Basic Study on a Soft Tactile Sensor Based on Subcutaneous Tissue with Collagen Fibers

2016 ◽  
pp. 35-43 ◽  
Author(s):  
Yuto Sonoi ◽  
Yoshihiro Tanaka ◽  
Masayoshi Hashimoto ◽  
Motoaki Fukasawa ◽  
Nobuteru Usuda ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Tactile Sensor ◽  
Collagen Fibers ◽  
Basic Study

scholarly journals Biocompatibility evaluation of a new bioresorbable pin for membrane fixation

2010 ◽  
Vol 21 (6) ◽  
pp. 482-490 ◽  
Author(s):  
Tania Mary Cestari ◽  
Rodrigo Cardoso de Oliveira ◽  
Jefferson Tomio Sanada ◽  
Gustavo Pompermaier Garlet ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Plasma Cells ◽  
Immune Reaction ◽  
Subcutaneous Tissue ◽  
Giant Cells ◽  
Collagen Fibers ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Bovine Bone ◽  
Microstructural Aspect ◽  
Alternative Material

The aim of this study was to morphometrically analyze the tissue response to a customized pin obtained from devitalized bovine cortical bone (DBCB-pin) implanted in the subcutaneous tissue of rats, as well as to assess its microstructural aspect by scanning electron microscopy (SEM). The pins were implanted in the subcutaneous tissue of 20 rats, which were killed at 7, 14, 28 and 60 days (5 rats/period) after implantation. In the subcutaneous tissue, DBCB-pin promoted the formation of a fibrous capsule. At 7 days, capsule showed thickness of 70 ± 3.2 µm with higher density of newly formed capillaries and smaller density of collagen fibers. Between 14 and 60 days, more organized fibrous capsule exhibited smaller thickness (53 ± 5.5 µm) with higher density of fibroblasts and collagen fibers. In this period, a small and slow bioresorption of the DBCB-pin by macrophages and rare multinucleated giant cells without tissue damage was observed. The thickness of DBCB-pin resorbed was in mean only of 9.3 µm. During all experimental periods not occurred presence of immune reaction cells as lymphocytes and plasma cells. It was concluded that the pin derived from cortical bovine bone was well tolerated by subcutaneous tissue of rats and slowly resorbed could be an alternative material for membrane fixation in the guided tissue regeneration procedures.

scholarly journals Rat subcutaneous tissue response to MTA Fillapex® and Portland cement

2013 ◽  
Vol 24 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Nádia Carolina Teixeira Marques ◽  
Natalino Lourenço Neto ◽  
Ana Paula Fernandes ◽  
Camila de Oliveira Rodini ◽  
Marco Antônio Hungaro Duarte ◽  
...  
Keyword(s):  
Portland Cement ◽  
Propylene Glycol ◽  
Inflammatory Reaction ◽  
Normal Tissue ◽  
Subcutaneous Tissue ◽  
Collagen Fibers ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Filling Material ◽  
Tissue Healing

The aim of this study was to evaluate the response of rat subcutaneous tissue to MTA Fillapex® (Angelus), an experimental root canal filling material based on Portland cement and propylene glycol (PCPG), and a zinc oxide, eugenol and iodoform (ZOEI) paste. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 7 and 15 days. The specimens were stained with hematoxylin and eosin, and evaluated regarding inflammatory reaction parameters by optical microscopy. The intensity of inflammatory response against the sealers was analyzed by two blinded and previously calibrated examiners for all experimental periods (kappa=0.96). The histological evaluation showed that all materials caused a moderate inflammatory reaction at 7 days, which subsided with time. A greater inflammatory reaction was observed at 7 days in the tubes filled with ZOEI paste. Tubes filled with MTA Fillapex presented some giant cells, macrophages and lymphocytes after 7 days. At 15 days, the presence of fibroblasts and collagen fibers was observed indicating normal tissue healing. The tubes filled with PCPG showed similar results to those observed in MTA Fillapex. At 15 days, the inflammatory reaction was almost absent at the tissue, with several collagen fibers indicating normal tissue healing. Data were analyzed by the nonparametric Kruskal-Wallis test (α=0.05). Statistically significant difference (p<0.05) was found only between PCPG at 15 days and ZOEI at 7 days groups. No significant differences were observed among the other groups/periods (p>0.05). MTA Fillapex and Portland cement added with propylene glycol had greater tissue compatibility than the PCPG paste.

Basic quantitative study on stress dispersion in subcutaneous tissue by the distribution of collagen fibers

2017 ◽  
Vol 2017.29 (0) ◽  
pp. 1D33
Author(s):  
Yoshihiro TANAKA ◽  
Masayoshi HASHIMOTO ◽  
Yuto SONOI ◽  
Motoaki FUKASAWA ◽  
Nobuteru USUDA ◽  
...  
Keyword(s):  
Quantitative Study ◽  
Subcutaneous Tissue ◽  
Collagen Fibers

A Study on FEM Analysis of a Flexible Tactile Sensor Using Structure of Subcutaneous Tissue

2018 ◽  
Vol 2018 (0) ◽  
pp. 1P1-K17
Author(s):  
Junpei NISHIZAWA ◽  
Yuto SONOI ◽  
Yoshihiro TANAKA ◽  
Motoaki FUKASAWA ◽  
Nobuteru USUDA ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Fem Analysis ◽  
Tactile Sensor

scholarly journals Biocompatibility, Biomineralization, and Maturation of Collagen by RTR®, Bioglass and DM Bone® Materials

2020 ◽  
Vol 31 (5) ◽  
pp. 477-484
Author(s):  
Francine Benetti ◽  
Carlos Roberto Emerenciano Bueno ◽  
Alexandre Henrique dos Reis-Prado ◽  
Marina Trevelin Souza ◽  
Juliana Goto ◽  
...  
Keyword(s):  
Collagen Fiber ◽  
Subcutaneous Tissue ◽  
Polarized Light ◽  
Collagen Fibers ◽  
Control Group ◽  
Tissue Replacement ◽  
Parametric Data ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract This study evaluated the biocompatibility, biomineralization, and collagen fiber maturation induced by Resorbable Tissue Replacement (RTR®; β-tricalcium phosphate [TCP]), Bioglass (BIOG; bioactive glass), and DM Bone® (DMB; hydroxyapatite and β-TCP) in vivo. Sixty-four polyethylene tubes with or without (control group; CG) materials (n=8/group/period) were randomly implanted in the subcutaneous tissue of 16 male Wistar rats (four per rat), weighting 250 to 280 g. The rats were killed after 7 and 30 days (n=8), and the specimens were removed for analysis of inflammation using hematoxylin-eosin; biomineralization assay using von Kossa (VK) staining and polarized light (PL); and collagen fiber maturation using picrosirius red (PSR). Nonparametric data were statistically analyzed by Kruskal-Wallis and Dunn tests, and parametric data by one-way ANOVA test (p<0.05). At 7 days, all groups induced moderate inflammation (p>0.05). At 30 days, there was mild inflammation in the BIOG and CG, and moderate inflammation in the RTR and DMB groups, with a significant difference between the CG and RTR (p<0.05). The fibrous capsule was thick at 7 days and predominantly thin at 30 days in all groups. All materials exhibited structures that stained positively for VK and PL. Immature collagen fibers were predominant at 7 and 30 days in all groups (p>0.05), although DMB exhibited more mature fibers than BIOG at 30 days (p<0.05). RTR, BIOG, and DMB were biocompatible, inducing inflammation that reduced over time and biomineralization in the subcutaneous tissue of rats. DMB exhibited more mature collagen fibers than BIOG over a longer period.

Methionine-Specific Staining of Collagen

1982 ◽  
Vol 40 ◽  
pp. 294-295
Author(s):  
E.M. Kuhn ◽  
K.D. Marenus ◽  
M. Beer
Keyword(s):  
Amino Acids ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Electron Microscopic ◽  
Good Correspondence ◽  
Specific Staining ◽  
Type Iii ◽  
Different Types ◽  
Sulfur Containing

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

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