Characterization of antimicrobial resistance of recentSalmonella entericaserovar Gallinarum isolates from chickens in South Korea

2010 ◽  
Vol 39 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Min S. Kang ◽  
Aeran Kim ◽  
Byeong Y. Jung ◽  
Moon Her ◽  
Wooseog Jeong ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
South Korea

scholarly journals 1667. Change in characteristics of community-onset ciprofloxacin-resistant E. coli isolates causing community-acquired acute pyelonephritis in South Korea

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S820-S820
Author(s):  
Bongyoung Kim ◽  
Ki Tae Kwon ◽  
Seong-yeol Ryu ◽  
Seong-Heon Wie ◽  
Hyun-uk Jo ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
South Korea ◽  
Molecular Characterization ◽  
Acute Pyelonephritis ◽  
Multilocus Sequence Typing ◽  
Quinolone Resistance ◽  
E Coli ◽  
Extended Spectrum ◽  
Community Onset

Abstract Background The aim of this study was to examine the change in characteristics of community-onset ciprofloxacin-resistant (CIP-R) E. coli isolates causing community-acquired acute pyelonephritis (CA-APN) in South Korea between 2010-2011 and 2017-2018. Methods E. coli samples isolated from the blood or urine were collected from patients with CA-APN aged 19 years and more who were admitted to 8 Korean hospitals from September 2017 to August 2018, prospectively. One isolate was collected from each patient. Phylogenetic typing, multilocus sequence typing (MLST), and molecular characterization of β-lactamase resistance and plasmid-mediated quinolone resistance (PMQR) determinants were performed. The data were compared with those from the previous study with same design in 2010-2011. Results A total of 346 and 300 isolates were collected during 2017-2018 and 2010-2011, respectively. Among them, 76 (22.0%) and 77 (25.7%) were CIP-R isolates. Significantly higher antimicrobial resistance against ampicillin (75.7% vs. 100%, P < 0.001) and cefotaxime (23.9% vs. 77.9%, P < 0.001) were observed for isolates in 2017-2018 compared to those in 2010-2011. The proportion of phylogenic group B2 had increased significantly (44.7% vs. 79.2%, P < 0.001). As for MLST, the proportion of ST131 (27.6% vs. 66.2%, P < 0.001) had increased while that of ST393 (18.4% vs. 3.9%, P =0.004) had decreased significantly. Higher proportion of CIP-R E. coli isolates in 2017-2018 had extended-spectrum β-lactamase (ESBL)/plasmid-mediated AmpC β-lactamase (PABL) (23.7% vs. 79.2%, P < 0.001) and PMQR determinant (11.8% vs. 40.8%, P < 0.001) compared to those in 2010-2011. Phlogenetic tree Analyzed by SplitsTree Conclusion Among uropathogenic CIP-R E. coli isolates in South Korea, ST131 predominance had become more prominent and the proportion of containing ESBL/PABL and/or PMQR determinants had increased. Disclosures All Authors: No reported disclosures

scholarly journals Antimicrobial Resistance and PFGE Molecular Typing of Salmonella enterica Serovar Gallinarum Isolates from Chickens in South Korea from 2013 to 2018

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 83
Author(s):  
Jun-Feng Zhang ◽  
Ke Shang ◽  
Jong-Yeol Park ◽  
Yea-Jin Lee ◽  
Yu-Ri Choi ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
South Korea ◽  
Control Strategies ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Cross Contamination ◽  
Antimicrobial Resistance Profile ◽  
Horizontal Spread ◽  
And Control

Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) genotypes of collected S. enterica ser. Gallinarum isolates were investigated to examine the epidemiological relationship between field outbreak isolates of S. enterica ser. Gallinarum. Thirty S. enterica ser. Gallinarum isolates collected from poultry farms with FT outbreaks from 2013 to 2018 in South Korea were analyzed. All isolates were resistant to at least 3 of the 18 antimicrobials tested and exhibited an MDR phenotype. All isolates showed resistance to streptomycin, sulfisoxazole, and colistin. One isolate was resistant to 9 antimicrobials. The antimicrobial resistance profile, streptomycin-sulfisoxazole-colistin-nalidixic acid-ciprofloxacin-gentamicin (18/30, 60.0%), was the most prevalent. PFGE types were classified into 10 groups with a 100% correlation cutoff in dendrograms for 30 field isolates. The dominant PFGE types were 1 (8/30, 26.7%), 4 (7/30, 23.3%), and 9 (5/30, 16.7%). Interestingly some isolates collected from the same and different companies had the same PFGE type. We reported a high MDR rate in S. enterica ser. Gallinarum isolates. The present study highlights the occurrence of horizontal spread and cyclic contamination of MDR S. enterica ser. Gallinarum within the same company. Furthermore, we showed cross-contamination between different companies. The characterization of these isolates would be helpful in the development of prevention and control strategies for MDR S. enterica ser. Gallinarum infection in South Korea.

Antimicrobial Resistance and Virulence Characterization of E. Coli Isolated from Subclinical Mastitic Sheep and Goats

2018 ◽  
Vol 34 (3) ◽  
pp. 267-278
Author(s):  
Ashraf A. Abd El-Tawab ◽  
Mohamed G. Aggour ◽  
Fatma I. El- Hofy ◽  
Marwa M. Y. El- Mesalami
Keyword(s):  
Antimicrobial Resistance ◽  
Sheep And Goats ◽  
E Coli

scholarly journals Genotypic Characterization of Antimicrobial Resistant Salmonella spp. Strains from Three Poultry Processing Plants in Colombia

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 491
Author(s):  
Alejandra Ramirez-Hernandez ◽  
Ana K. Carrascal-Camacho ◽  
Andrea Varón-García ◽  
Mindy M. Brashears ◽  
Marcos X. Sanchez-Plata
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Spp ◽  
Microbial Profile ◽  
Antimicrobial Resistance Genes ◽  
Processing Plants ◽  
Poultry Processing ◽  
Paratyphi B ◽  
And Control ◽  
Sequence Types

The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicrobial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Additionally, whole-genome sequencing was conducted to characterize the isolated strains genotypically. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six distinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.

scholarly journals Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance

2021 ◽  
Vol 22 (1) ◽  
pp. 456
Author(s):  
Simone Rentschler ◽  
Lars Kaiser ◽  
Hans-Peter Deigner
Keyword(s):  
Antimicrobial Resistance ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Rapid Identification ◽  
Adequate Treatment ◽  
Resistance Patterns ◽  
Detection And Diagnosis ◽  
Patient Prognosis ◽  
Point Of Care Detection

Precise and rapid identification and characterization of pathogens and antimicrobial resistance patterns are critical for the adequate treatment of infections, which represent an increasing problem in intensive care medicine. The current situation remains far from satisfactory in terms of turnaround times and overall efficacy. Application of an ineffective antimicrobial agent or the unnecessary use of broad-spectrum antibiotics worsens the patient prognosis and further accelerates the generation of resistant mutants. Here, we provide an overview that includes an evaluation and comparison of existing tools used to diagnose bacterial infections, together with a consideration of the underlying molecular principles and technologies. Special emphasis is placed on emerging developments that may lead to significant improvements in point of care detection and diagnosis of multi-resistant pathogens, and new directions that may be used to guide antibiotic therapy.

scholarly journals Risk characterization of antimicrobial resistance of Salmonella in meat products

Food Control ◽  
2015 ◽  
Vol 57 ◽  
pp. 18-23 ◽  
Author(s):  
Eva Doménech ◽  
Ana Jiménez-Belenguer ◽  
Rosa Pérez ◽  
María Antonia Ferrús ◽  
Isabel Escriche
Keyword(s):  
Antimicrobial Resistance ◽  
Meat Products ◽  
Risk Characterization

scholarly journals Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1163
Author(s):  
So-Youn Youn ◽  
Ji-Youn Lee ◽  
You-Chan Bae ◽  
Yong-Kuk Kwon ◽  
Hye-Ryoung Kim
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Prevention ◽  
South Korea ◽  
Prevention And Control ◽  
Genetic Recombination ◽  
Detection Methods ◽  
Infectious Bronchitis ◽  
Reference Sequences ◽  
And Control

Infectious bronchitis viruses (IBVs) are evolving continuously via genetic drift and genetic recombination, making disease prevention and control difficult. In this study, we undertook genetic and pathogenic characterization of recombinant IBVs isolated from chickens in South Korea between 2003 and 2019. Phylogenetic analysis showed that 46 IBV isolates belonged to GI-19, which includes nephropathogenic IBVs. Ten isolates formed a new cluster, the genomic sequences of which were different from those of reference sequences. Recombination events in the S1 gene were identified, with putative parental strains identified as QX-like, KM91-like, and GI-15. Recombination detection methods identified three patterns (rGI-19-I, rGI-19-II, and rGI-19-III). To better understand the pathogenicity of recombinant IBVs, we compared the pathogenicity of GI-19 with that of the rGI-19s. The results suggest that rGI-19s may be more likely to cause trachea infections than GI-19, whereas rGI-19s were less pathogenic in the kidney. Additionally, the pathogenicity of rGI-19s varied according to the genotype of the major parent. These results indicate that genetic recombination between heterologous strains belonging to different genotypes has occurred, resulting in the emergence of new recombinant IBVs in South Korea.

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