The effect of freeze-drying and storage on lysozyme activity, lactoferrin content, superoxide dismutase activity, total antioxidant capacity and fatty acid profile of freeze-dried human milk

2020 ◽  
pp. 1-11
Author(s):  
Dorota Martysiak-Żurowska ◽  
Patrycja Rożek ◽  
Małgorzata Puta
Keyword(s):  
Superoxide Dismutase ◽  
Fatty Acid ◽  
Human Milk ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Freeze Drying ◽  
Lysozyme Activity ◽  
Freeze Dried ◽  
Total Antioxidant ◽  
And Storage

scholarly journals The effect of lyophilization on selected biologically active components (vitamin C, catalase, lysozyme), total antioxidant capacity and lipid oxidation in human milk

2017 ◽  
Vol 112 (3) ◽  
pp. 121-128
Author(s):  
Dorota Martysiak-Żurowska ◽  
Małgorzata Puta ◽  
Anna Rodzik ◽  
Edyta Malinowska-Pańczyk
Keyword(s):  
Vitamin C ◽  
Human Milk ◽  
Lipid Oxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Freeze Drying ◽  
Biologically Active ◽  
Initial Water Content ◽  
Initial Water ◽  
Total Antioxidant

Human milk is rich in polyunsaturated fatty acids, as well as lysozyme, vitamin C and other bioactive compounds. The effect of lyophilization on the content of antioxidants (vitamin C and catalase CAT), bactericidal compounds (lysozyme), total antioxidant capacity (TAC) and lipid peroxidation in human milk was investigated in this study. Samples of mature human milk were collected from five healthy women who gave birth on the scheduled date and without complications. Freeze drying resulted in the removal of 88.2 % of the initial water content from milk. The human milk lyophilizate was readily soluble in water. Lyophilization had no effect on the content of primary (lipid peroxides LP) and secondary (thiobarbituric acid reactive substances TBARS) products of lipid oxidation. Freeze-drying led to a significant decrease in the vitamin C content and TAC values of milk (by 31 % and 16.5 %, respectively). Catalase and lysozyme were resistant to freeze-drying. Lyophilization induced a decrease in lysozyme content (9 %) and catalase activity (11 %) but these changes were not statistically significant. Low-temperature dehydration and rehydration of human milk lyophilizates promote satisfactory retention of biologically active ingredients and prevent the oxidation of human milk lipids. The results of this study indicate that lyophilization can be considered as an effective method for prolonging the shelf life of human milk.

The Effects of Individual Vitamin and Mineral Complex on Some Parameters of Physical Capacity and Blood Oxidative Metabolism in Athletes

2015 ◽  
Vol 22 (4) ◽  
pp. 127-131
Author(s):  
Мартусевич ◽  
A. Martusevich ◽  
Карузин ◽  
K. Karuzin
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Oxidative Metabolism ◽  
Total Antioxidant Capacity ◽  
Physical Capacity ◽  
Mineral Complex ◽  
Total Antioxidant ◽  
Oxidative Processes ◽  
The Individual ◽  
Positive Effect

The purpose of this work was the estimation of the effects of the individual technology of metabolic correction on the parameters of the physical capacity and blood oxidative processes in athletes. The authors studied physical capacity indexes and blood oxidative metabolism (by total antioxidant capacity and activity of superoxide dismutase) in 24 athletes of main group (with vitamin and mineral complex) and 30 sportsmen, which got a placebo. It was stated the positive effect of investigated complex on the physical capacity, is realized through the directed optimization of the blood oxidative metabolism, first of all – antioxidant enzymes.

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