Changes in serum virus-specific IgM/IgG antibody in asymptomatic and discharged patients with reoccurring positive COVID-19 nucleic acid test (RPNAT)

Abstract Background: Coronavirus disease 2019 (COVID-19) has spread rapidly in China and globally. In order to control the spread of the epidemic, it is important to find an efficient diagnostic method. Objectives: The aim of this study was to assess the responses of antibodies during SARS-CoV-2 infection in relation to disease severity and to evaluate the association between the positive rate of antibody detection and nucleic acid test. Methods: Ninety patients with SARS-CoV-2 infection were recruited in this retrospective observational study. Demographic, clinical data, and SARS-CoV-2 IgM and IgG antibodies in serum specimens were detected at 4 and 6 weeks after diagnosis. Results: IgM and IgG antibody levels showed a decreased tendency, the titers at week 4 were higher than the titers at week 6: The positive rates of IgM at week 4 and 6 were 92.9% and 67.9%, respectively. The positive rates of IgG at week 4 and week 6 were 100%. No association was found between the positive rate of antibody detection at week 4 or 6 and that of nucleic acid test (P>0.05). No difference between the positive rate of antibodies against SARS-CoV-2 in severe and non-severe COVID-19 patients was observed. Conclusions: Antibody detection is an effective means in the diagnosis of COVID-19. The titer and positive rate of IgM are lower than those of IgG in the first six weeks after infection. Positive rate of antibodies was not different between severe and non-severe patients.

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The Long-Lasting Persistence of SARS-CoV-2 Nucleic Acid in COVID-19 Patients

10.21203/rs.3.rs-354369/v1 ◽

2021 ◽

Author(s):

Shan Li ◽

Chaojie Wu ◽

Mingshun Zhang ◽

Lianzheng Zhou ◽

Jie Xiang ◽

...

Keyword(s):

Nucleic Acid ◽

Severe Acute Respiratory Syndrome ◽

Disease Course ◽

Nucleic Acid Test ◽

Discharged Patients ◽

Case Presentations ◽

Underlying Diseases ◽

Acid Test

Abstract Background: Although recurring cases of COVID-19 have been sporadically reported, the long-persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still arguable. We aimed to provide the evidences of recovered COVID-19 patients with long-lasting positive SARS-CoV-2 RNA tests in a Chinese hospital. Case presentations: We reported 4 discharged patients with COVID-19 patients relapsed during the period of self-quarantine, leading to an extended disease course. All of four patients were historically healthy without known underlying diseases (diabetes, hypertension, cancer, i.e.) which may influence the disease course. Conclusion: The persistent course of SARS-COV-2 nucleic acid test positive in these 4 cases was prolonged, which provided some ideas for the long-term existence of SARS-COV-2 RNA in convalescent patients.

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Management strategy of Corona Virus Disease-2019 in quarantine zones outside hospitals: Analysis of 1232 cases from a district in Wuhan

10.21203/rs.3.rs-28749/v1 ◽

2020 ◽

Author(s):

Xiaojian Zhu ◽

Jue Wang ◽

Hao Wang ◽

Yutong Tang ◽

Shu Zhou ◽

...

Keyword(s):

Nucleic Acid ◽

Management Strategy ◽

Virus Disease ◽

Cardinal Number ◽

Nucleic Acid Test ◽

Corona Virus ◽

Global Pandemic ◽

Positive Rate ◽

Discharged Patients ◽

Acid Test

Abstract The Corona Virus Disease 2019 (COVID-19) has evolved into a global pandemic in the early 2020. Management strategy outside hospitals of the suspected cases, close contacts and discharged patients might be as important as treatment in hospital. We analyzed information from 1232 cases at 14 hotels (requisitioned as quarantine zones) in Qiaokou district, Wuhan during Feb 8th to Mar 4th 2020. Abide by the unquarantine and hospitalization standard, 603 (48.94%) cases were released from quarantine zones; 540 (43.83%) cases were sent to hospital for further medical care. 89 (7.22%) cases remained on quarantine up to the end of the analysis. The reasons for cases sent to the hospital for treatment were either positive for COVID-19 nucleic acid test, progression in pulmonary CT scan, or aggravation of symptoms. 11/59 patients switched from negative to positive for nucleic acid test during stayed in quarantine zones after being discharged from the hospitals. In total, hospitalization and positive rate for COVID-19 nucleic acid test both decreased over time. The quarantine measures were important and played a pivotal role in identification of cardinal number, cutting off the transmission, reducing the scope of prevention and rehabilitation therapy. This protocol adopted in Wuhan provided countries worldwide with valuable experience.

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Clinical Significance of an IgM and IgG Test for Diagnosis of Highly Suspected COVID-19

Frontiers in Medicine ◽

10.3389/fmed.2021.569266 ◽

2021 ◽

Vol 8 ◽

Author(s):

Xingwang Jia ◽

Pengjun Zhang ◽

Yaping Tian ◽

Junli Wang ◽

Huadong Zeng ◽

...

Keyword(s):

Nucleic Acid ◽

Clinical Significance ◽

Clinical Symptoms ◽

Treatment Protocol ◽

Antibody Detection ◽

Igg Antibody ◽

Epidemic Disease ◽

Detection Rates ◽

Nucleic Acid Test ◽

Acid Test

Background: Nucleic acid detection and CT scanning have been reported in COVID-19 diagnosis. Here, we aimed to investigate the clinical significance of IgM and IgG testing for the diagnosis of highly suspected COVID-19.Methods: A total of 63 patients with suspected COVID-19 were observed, 57 of whom were enrolled (24 males and 33 females). The selection was based on the diagnosis and treatment protocol for COVID-19 (trial Sixth Edition) released by the National Health Commission of the People's Republic of China. Patients were divided into positive and negative groups according to the first nucleic acid results from pharyngeal swab tests. Routine blood tests were detected on the second day after each patient was hospitalized. The remaining serum samples were used for detection of novel coronavirus-specific IgM/IgG antibodies.Results: The rate of COVID-19 nucleic acid positivity was 42.10%. The positive detection rates with a combination of IgM and IgG testing for patients with COVID-19 negative and positive nucleic acid test results were 72.73 and 87.50%, respectively.Conclusions: We report a rapid, simple, and accurate detection method for patients with suspected COVID-19 and for on-site screening for close contacts within the population. IgM and IgG antibody detection can identify COVID-19 after a negative nucleic acid test. Diagnostic accuracy of COVID-19 might be improved by nucleic acid testing in patients with a history of epidemic disease or with clinical symptoms, as well as CT scans when necessary, and serum-specific IgM and IgG antibody testing after the window period.

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Metode Nucleic Acid Test untuk Uji Saring Virus Hepatitis B pada Darah Donor dengan Hepatitis B Occult

Jurnal Biotek Medisiana Indonesia ◽

10.22435/jbmi.v4i2.5123.51-58 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Ulfah Suryani ◽

Vivi Setiawaty

Keyword(s):

Nucleic Acid ◽

Hepatitis B ◽

Virus Hepatitis ◽

Nucleic Acid Test ◽

Acid Test

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Cost-effectiveness of nucleic acid test screening of volunteer blood donations for hepatitis B, hepatitis C and human immunodeficiency virus in the United States

Vox Sanguinis ◽

10.1111/j.0042-9007.2004.00379.x ◽

2004 ◽

Vol 86 (1) ◽

pp. 28-40 ◽

Cited By ~ 99

Author(s):

D. A. Marshall ◽

S. H. Kleinman ◽

J. B. Wong ◽

J. P. AuBuchon ◽

D. T. Grima ◽

...

Keyword(s):

United States ◽

Human Immunodeficiency Virus ◽

Cost Effectiveness ◽

Hepatitis C ◽

Nucleic Acid ◽

Hepatitis B ◽

The United States ◽

Nucleic Acid Test ◽

Immunodeficiency Virus ◽

Acid Test

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What is the meaning of a nonresolved viral nucleic acid test-reactive minipool?

Transfusion ◽

10.1111/trf.12818 ◽

2014 ◽

Vol 55 (2) ◽

pp. 395-404 ◽

Cited By ~ 4

Author(s):

Lunan Wang ◽

Le Chang ◽

Yunzheng Xie ◽

Chengyin Huang ◽

Lei Xu ◽

...

Keyword(s):

Nucleic Acid ◽

Viral Nucleic Acid ◽

Nucleic Acid Test ◽

Acid Test

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Cases Report of Cured Novel Coronavirus-infected Pneumonia Patients with Viral Nucleic Acid Test Positive in Fecal Specimens

10.21203/rs.3.rs-20799/v1 ◽

2020 ◽

Author(s):

Mei Han ◽

Jingbo Zou ◽

Wenguang Tian ◽

Xiaoyu Wei ◽

Yang Zhou ◽

...

Keyword(s):

Nucleic Acid ◽

Molecular Diagnostic ◽

Clinical Practices ◽

Rt Pcr ◽

Viral Nucleic Acid ◽

Nucleic Acid Test ◽

Assessment Standard ◽

Novel Coronavirus ◽

Acid Test ◽

Respiratory Specimens

Abstract Background: The outbreak of the novel coronavirus in China (COVID-19) represents a significant and urgent threat to global health. We report here five cases of COVID-19 infection patients in our clinical practices who are medically stable and presumed to successfully “cleared” the virus after antiviral treatments. Case presentation: The clinical evaluation depends on the viral nucleic acid test in respiratory specimens by real-time PCR reverse transcription (RT-PCR) assays according to the authorized guidance. We found that the stool samples of these cured patients remain positive in RT-PCR assay while the virus is undetectable in respiratory specimens. RT-PCR molecular diagnostic assay was designed to specifically detect the presence of viral RNA. Thus, the positive result in the fecal specimens implies the existence of viable virions with the patients. Conclusions: This highlights the importance to look closely at the assessment standard of medical treatment, as well as the need for reevaluation of the criteria for the initial screening, prevention, and care of patients with this emerging infection.

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