Clinical Significance of an IgM and IgG Test for Diagnosis of Highly Suspected COVID-19

Background: Nucleic acid detection and CT scanning have been reported in COVID-19 diagnosis. Here, we aimed to investigate the clinical significance of IgM and IgG testing for the diagnosis of highly suspected COVID-19.Methods: A total of 63 patients with suspected COVID-19 were observed, 57 of whom were enrolled (24 males and 33 females). The selection was based on the diagnosis and treatment protocol for COVID-19 (trial Sixth Edition) released by the National Health Commission of the People's Republic of China. Patients were divided into positive and negative groups according to the first nucleic acid results from pharyngeal swab tests. Routine blood tests were detected on the second day after each patient was hospitalized. The remaining serum samples were used for detection of novel coronavirus-specific IgM/IgG antibodies.Results: The rate of COVID-19 nucleic acid positivity was 42.10%. The positive detection rates with a combination of IgM and IgG testing for patients with COVID-19 negative and positive nucleic acid test results were 72.73 and 87.50%, respectively.Conclusions: We report a rapid, simple, and accurate detection method for patients with suspected COVID-19 and for on-site screening for close contacts within the population. IgM and IgG antibody detection can identify COVID-19 after a negative nucleic acid test. Diagnostic accuracy of COVID-19 might be improved by nucleic acid testing in patients with a history of epidemic disease or with clinical symptoms, as well as CT scans when necessary, and serum-specific IgM and IgG antibody testing after the window period.

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The positive rate of IgM and IgG antibodies against SARS-CoV-2 is similar in severe and non-severe COVID-19 patients

Revista Romana de Medicina de Laborator ◽

10.2478/rrlm-2021-0010 ◽

2021 ◽

Vol 29 (1) ◽

pp. 85-91

Author(s):

Nan Jiang ◽

Yaoyao Sun ◽

Hongyan Sun ◽

Bo Yang ◽

Juan Tan ◽

...

Keyword(s):

Nucleic Acid ◽

Diagnostic Method ◽

Effective Means ◽

Antibody Detection ◽

Igg Antibodies ◽

Igg Antibody ◽

Nucleic Acid Test ◽

Positive Rate ◽

Antibody Levels ◽

Acid Test

Abstract Background: Coronavirus disease 2019 (COVID-19) has spread rapidly in China and globally. In order to control the spread of the epidemic, it is important to find an efficient diagnostic method. Objectives: The aim of this study was to assess the responses of antibodies during SARS-CoV-2 infection in relation to disease severity and to evaluate the association between the positive rate of antibody detection and nucleic acid test. Methods: Ninety patients with SARS-CoV-2 infection were recruited in this retrospective observational study. Demographic, clinical data, and SARS-CoV-2 IgM and IgG antibodies in serum specimens were detected at 4 and 6 weeks after diagnosis. Results: IgM and IgG antibody levels showed a decreased tendency, the titers at week 4 were higher than the titers at week 6: The positive rates of IgM at week 4 and 6 were 92.9% and 67.9%, respectively. The positive rates of IgG at week 4 and week 6 were 100%. No association was found between the positive rate of antibody detection at week 4 or 6 and that of nucleic acid test (P>0.05). No difference between the positive rate of antibodies against SARS-CoV-2 in severe and non-severe COVID-19 patients was observed. Conclusions: Antibody detection is an effective means in the diagnosis of COVID-19. The titer and positive rate of IgM are lower than those of IgG in the first six weeks after infection. Positive rate of antibodies was not different between severe and non-severe patients.

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IgM/IgG antibody changes in asymptomatic and discharged patients with reoccurring positive nucleic acid test (RP) of Novel Coronavirus Disease 2019 (COVID-19)

10.22541/au.159108671.13858622 ◽

2020 ◽

Author(s):

Junli Liu ◽

Rui Lian ◽

Guochao Zhang ◽

Baojun Hou ◽

Chuming Wang ◽

...

Keyword(s):

Nucleic Acid ◽

Igg Antibody ◽

Nucleic Acid Test ◽

Discharged Patients ◽

Novel Coronavirus ◽

Acid Test

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Experience with a triplex arbovirus nucleic acid test (NAT) at a Canadian Public Health Laboratory

BMC Infectious Diseases ◽

10.1186/s12879-021-06842-w ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Saugata Choudhury ◽

Raymond Tellier ◽

Kevin Fonseca ◽

Byron M. Berenger

Keyword(s):

Nucleic Acid ◽

Dengue Virus ◽

Symptom Onset ◽

Zika Virus ◽

Clinical Symptoms ◽

Clinical History ◽

Nucleic Acid Test ◽

Virus Rna ◽

Prolonged Duration ◽

Acid Test

Abstract Background Dengue, chikungunya and zika infections occur in tropical and subtropical regions of the world. We describe the utilization of an in-house nucleic acid test (NAT) targeting all three viruses for febrile returning travelers in Alberta, Canada. Methods NAT was performed until 40 days from symptom onset or exposure due to the prolonged duration of zika virus RNA detection. From Sept 1, 2017 to August 31, 2019, 2552 specimens from 1932 patients were tested. Results Approximately 2% of patients tested were NAT positive for dengue virus (n = 42), chikungunya virus (n = 4), and zika virus (n = 1). The majority presented with fever, myalgia and rash. Regions with the most frequent travel included SouthEast Asia (68.5%), South America (25%) and the Caribbean (6.5%). Ct values were stronger (~ 1.5 logs) for patients within 1–3 days following onset of clinical symptoms than those presenting later. Nineteen patients had urine and plasma submitted; 5 were positive for both specimens and 2 were positive only for dengue virus in the urine. Also, Ct values were lower for plasma when compared to the corresponding urine. RNA was detected until 10 days and 5 days post-exposure in plasma and urine respectively for dengue virus. Conclusions Owing to dengue viremia detected beyond the conventional 7 days and low levels of circulating zika virus globally, a cutoff of 14 days from symptom onset to NAT is sufficient to diagnose acute cases. Inclusion of a zoonotic history form that collects appropriate clinical history results in improved test utilization.

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Changes in serum virus-specific IgM/IgG antibody in asymptomatic and discharged patients with reoccurring positive COVID-19 nucleic acid test (RPNAT)

Annals of Medicine ◽

10.1080/07853890.2020.1811887 ◽

2020 ◽

Vol 53 (1) ◽

pp. 34-42 ◽

Cited By ~ 2

Author(s):

Junli Liu ◽

Rui Lian ◽

Guochao Zhang ◽

Baojun Hou ◽

Chuming Wang ◽

...

Keyword(s):

Nucleic Acid ◽

Igg Antibody ◽

Nucleic Acid Test ◽

Discharged Patients ◽

Acid Test

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Metode Nucleic Acid Test untuk Uji Saring Virus Hepatitis B pada Darah Donor dengan Hepatitis B Occult

Jurnal Biotek Medisiana Indonesia ◽

10.22435/jbmi.v4i2.5123.51-58 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Ulfah Suryani ◽

Vivi Setiawaty

Keyword(s):

Nucleic Acid ◽

Hepatitis B ◽

Virus Hepatitis ◽

Nucleic Acid Test ◽

Acid Test

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Clinical characteristics and outcomes of COVID-19 long-term nucleic acid positive patients

Technology and Health Care ◽

10.3233/thc-212921 ◽

2021 ◽

pp. 1-10

Author(s):

Xuan Zhu ◽

Xinxin Zhu ◽

Min Wang ◽

Fang Yang ◽

Zhibing Sun ◽

...

Keyword(s):

Nucleic Acid ◽

Clinical Characteristics ◽

Clinical Symptoms ◽

Control Group ◽

Viral Shedding ◽

Imaging Characteristics ◽

And Gender ◽

Acid Test ◽

Pulmonary Ct

OBJECTIVE: This study aimed to investigate the clinical characteristics and outcomes of coronavirus disease-19 (COVID-19) long-term nucleic acid positive patients (hereinafter referred to as CLTAPs). METHODS: Patients were recruited from the Xiaogan Central Hospital between 16 January 2020 and 28 March 2020. Among the 562 cases of patients with laboratory-identified COVID-19 infection by real-time polymerase chain reaction (qtPCR), 19 cases of COVID-19 patients with more than 41 days from the first to the last time of nucleic acid test were selected as the study group, and 76 cases of age- and gender-matched COVID-19 patients were selected as the control group (hereinafter referred to as C-CLTAPs). Demographic characteristics, clinical symptoms, laboratory examination and computed tomography (CT) imaging characteristics were retrospectively analyzed. RESULTS: On admission, among the 562 cases of patients with COVID-19, there were 398 cases of ordinary COVID-19 patients, 99 cases of severe COVID-19 patients and 99 cases of critical COVID-19 patients. CLTAPs had milder clinical symptoms and longer viral shedding time in comparison to C-CLTAPs. Compared to C-CLTAPs, CLTAPs had a lower infection index at admission. CLTAPs used less oxygen therapy and a higher proportion of hydroxychloroquine treatment in comparison to C-CLTAPs. In comparison to C-CLTAPs, CLTAPs showed slower pulmonary CT progression and faster pulmonary CT absorption. CONCLUSION: In this study, out of the 562 cases, we found 19 CLTAPs. The clinical differences between CLTAPs and C-CLTAPs were compared and analyzed. We hope that these finding can provide a theoretical basis for the treatment of CLTAPs.

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Cost-effectiveness of nucleic acid test screening of volunteer blood donations for hepatitis B, hepatitis C and human immunodeficiency virus in the United States

Vox Sanguinis ◽

10.1111/j.0042-9007.2004.00379.x ◽

2004 ◽

Vol 86 (1) ◽

pp. 28-40 ◽

Cited By ~ 99

Author(s):

D. A. Marshall ◽

S. H. Kleinman ◽

J. B. Wong ◽

J. P. AuBuchon ◽

D. T. Grima ◽

...

Keyword(s):

United States ◽

Human Immunodeficiency Virus ◽

Cost Effectiveness ◽

Hepatitis C ◽

Nucleic Acid ◽

Hepatitis B ◽

The United States ◽

Nucleic Acid Test ◽

Immunodeficiency Virus ◽

Acid Test

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What is the meaning of a nonresolved viral nucleic acid test-reactive minipool?

Transfusion ◽

10.1111/trf.12818 ◽

2014 ◽

Vol 55 (2) ◽

pp. 395-404 ◽

Cited By ~ 4

Author(s):

Lunan Wang ◽

Le Chang ◽

Yunzheng Xie ◽

Chengyin Huang ◽

Lei Xu ◽

...

Keyword(s):

Nucleic Acid ◽

Viral Nucleic Acid ◽

Nucleic Acid Test ◽

Acid Test

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Cases Report of Cured Novel Coronavirus-infected Pneumonia Patients with Viral Nucleic Acid Test Positive in Fecal Specimens

10.21203/rs.3.rs-20799/v1 ◽

2020 ◽

Author(s):

Mei Han ◽

Jingbo Zou ◽

Wenguang Tian ◽

Xiaoyu Wei ◽

Yang Zhou ◽

...

Keyword(s):

Nucleic Acid ◽

Molecular Diagnostic ◽

Clinical Practices ◽

Rt Pcr ◽

Viral Nucleic Acid ◽

Nucleic Acid Test ◽

Assessment Standard ◽

Novel Coronavirus ◽

Acid Test ◽

Respiratory Specimens

Abstract Background: The outbreak of the novel coronavirus in China (COVID-19) represents a significant and urgent threat to global health. We report here five cases of COVID-19 infection patients in our clinical practices who are medically stable and presumed to successfully “cleared” the virus after antiviral treatments. Case presentation: The clinical evaluation depends on the viral nucleic acid test in respiratory specimens by real-time PCR reverse transcription (RT-PCR) assays according to the authorized guidance. We found that the stool samples of these cured patients remain positive in RT-PCR assay while the virus is undetectable in respiratory specimens. RT-PCR molecular diagnostic assay was designed to specifically detect the presence of viral RNA. Thus, the positive result in the fecal specimens implies the existence of viable virions with the patients. Conclusions: This highlights the importance to look closely at the assessment standard of medical treatment, as well as the need for reevaluation of the criteria for the initial screening, prevention, and care of patients with this emerging infection.

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