Comparison of surface properties, cell behaviors, bone regeneration and osseointegration between nano tantalum/PEEK composite and nano silicon nitride/PEEK composite

Polyetheretherketone (PEEK) is a potential substitute for conventional metallic biomedical implants owing to its superior mechanical and chemical properties, as well as biocompatibility. However, its inherent bio-inertness and poor osseointegration limit its use in clinical applications. Herein, thin titanium films were deposited on the PEEK substrate by plasma sputtering, and porous nanonetwork structures were incorporated on the PEEK surface by alkali treatment (PEEK-TNS). Changes in the physical and chemical characteristics of the PEEK surface were analyzed to establish the interactions with cell behaviors. The osteoimmunomodulatory properties were evaluated using macrophage cells and osteoblast lineage cells. The functionalized nanostructured surface of PEEK-TNS effectively promoted initial cell adhesion and proliferation, suppressed inflammatory responses, and induced macrophages to anti-inflammatory M2 polarization. Compared with PEEK, PEEK-TNS provided a more beneficial osteoimmune environment, including increased levels of osteogenic, angiogenic, and fibrogenic gene expression, and balanced osteoclast activities. Furthermore, the crosstalk between macrophages and osteoblast cells showed that PEEK-TNS could provide favorable osteoimmunodulatory environment for bone regeneration. PEEK-TNS exhibited high osteogenic activity, as indicated by alkaline phosphatase activity, osteogenic factor production, and the osteogenesis/osteoclastogenesis-related gene expression of osteoblasts. The study establishes that the fabrication of titanate nanonetwork structures on PEEK surfaces could extract an adequate immune response and favorable osteogenesis for functional bone regeneration. Furthermore, it indicates the potential of PEEK-TNS in implant applications.

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Prepared and Surface Analyzed of Nano-Silicon Nitride Thin Films

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.233-235.2015 ◽

2011 ◽

Vol 233-235 ◽

pp. 2015-2018

Author(s):

Gui Wen Yu ◽

Jing Dong ◽

Ye Tian ◽

Wen Xin Li ◽

Xue Gong

Keyword(s):

Thin Films ◽

Silicon Nitride ◽

Deposition Rate ◽

Room Temperature ◽

Atomic Ratio ◽

Sputtering Deposition ◽

Depth Profiles ◽

Silicon Nitride Films ◽

Nano Silicon ◽

Temperature Depth

Thin silicon nitride films were prepared on PET by r.f. reactive sputtering. Deposition Rate, reactive mechanisms, the thickness attribution, chemical stoichiometry and impurity were studied by means of RBS, XPS, and ellipsometer. Results show that chemical stoichiometric films with N-to-Si atomic ratio of 4:3 were achieved even at room temperature. Depth profiles of XPS and SIMS reveal that oxide exists only at the interface between nitride and substrate and Ar atoms are buried in the films.

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Bioengineered mussel glue incorporated with a cell recognition motif as an osteostimulating bone adhesive for titanium implants

Journal of Materials Chemistry B ◽

10.1039/c5tb01230a ◽

2015 ◽

Vol 3 (41) ◽

pp. 8102-8114 ◽

Cited By ~ 17

Author(s):

Yun Kee Jo ◽

Bong-Hyuk Choi ◽

Cong Zhou ◽

Jin-Soo Ahn ◽

Sang Ho Jun ◽

...

Keyword(s):

Bone Regeneration ◽

Titanium Implants ◽

Osteoblastic Cell ◽

Cell Recognition ◽

Cell Behaviors ◽

Recognition Motif ◽

A Cell

An engineered mussel glue MAP-RGD can be successfully used as a novel functional osteostimulating bone adhesive for titanium implants through improved osteoblastic cell behaviors, blood responses, and eventually enhanced bone regeneration.

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Enhancement of Bone Regeneration on Calcium-Phosphate-Coated Magnesium Mesh: Using the Rat Calvarial Model

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.652334 ◽

2021 ◽

Vol 9 ◽

Author(s):

Shuang Wu ◽

Yong-Seok Jang ◽

Min-Ho Lee

Keyword(s):

Bone Regeneration ◽

Surface Properties ◽

Critical Size ◽

Calcium Phosphates ◽

Cell Seeding ◽

Critical Size Defect ◽

Direct Cell ◽

Cell Affinity ◽

Biodegradable Magnesium

Metallic biodegradable magnesium (Mg) is a promising material in the biomedical field owing to its excellent biocompatibility, bioabsorbability, and biomechanical characteristics. Calcium phosphates (CaPs) were coated on the surface of pure Mg through a simple alkali-hydrothermal treatment. The surface properties of CaP coatings formed on Mg were identified through wettability, direct cell seeding, and release tests since the surface properties of biomaterials can affect the reaction of the host tissue. The effect of CaP-coated Mg mesh on guided bone regeneration in rat calvaria with the critical-size defect was also evaluated in vivo using several comprehensive analyses in comparison with untreated Mg mesh. Following the application of protective CaP coating, the surface energy of Mg improved with higher hydrophilicity and cell affinity. At the same time, the CaP coating endowed Mg with higher Ca affinity and lower degradation. The Mg mesh with CaP coating had higher osteointegration and bone affinity than pristine Mg mesh.

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