Screening for Antifungal Activities of Some Medicinal Plants used Traditionally in Saudi Arabia

Abstract To prevent foodborne diseases and extend shelf-life, antimicrobial agents may be used in food to inhibit the growth of undesired microorganisms. The present study was aimed to determine the antimicrobial and antifungal activities of the fermented medicinal plants extract using Lactobacillus acidophilus ATCC 4356. The fermentation kinetic parameters, biochemical composition and the volatile compounds of the fermented plant extract was assessed. The results showed that, the fermented plants beverage exhibited high content in polyphenols, flavonoids, and tannins (152.7 mg AGE/ L; 93.6 mg RE/ L; and 62.1mg CE/ L, respectively) to compare with the extract without fermentation. The GC-MS headspace analyses showed the presence of twenty-four interesting volatile compounds. The richness of the fermented plants extracts of polyphenols content and the bioactive compound such as Eucalyptol, Camphene, α-Phellandrene, α-Terpinene improve their biological activity. In addition, the fermented plants extract exhibited a high antimicrobial potential against resistance pathogenic bacteria and fungi determined by different methods. The maximum inhibition showed in the fermented plants beverage against Escherichia coli 25922/3, Pseudomonas aeruginosa 27853 ATCC, Staphylococcus aureus 29213 ATCC, Enterococcus aerogenes 13048 ATCC, Phytophthora infestans P3 4/91 R+, Phytophthora infestans P4 20/01 R, Phytophthora infestans (GL-1). The obtained results support the hypothesis of using whey as a functional ingredient to improve food preservation. The bioprocesses of fermentation technology enhance antimicrobial and antifungal activities which could be used in different industrial applications.

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ANTICANCER POTENTIAL OF PLANT EXTRACTS FROM RIYADH (SAUDI ARABIA) ON MDA-MB-231 BREAST CANCER CELLS

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcam.v15i4.7 ◽

2018 ◽

Vol 15 (4) ◽

pp. 46 ◽

Cited By ~ 1

Author(s):

Fahd A. Nasr ◽

Nael Abutaha ◽

Mohammad Al-Zahrani ◽

Muhammad Farooq ◽

Mohammad A Wadaan

Keyword(s):

Breast Cancer ◽

Saudi Arabia ◽

Medicinal Plants ◽

Traditional Medicine ◽

Anticancer Activity ◽

Cancer Cells ◽

Anticancer Agents ◽

Breast Cancer Cells ◽

Morphological Changes ◽

Cell Integrity

Background: Medicinal plants have been used in traditional medicine for the treatment of numerous diseases worldwide. There is a dire need for new anticancer agents and plants used in traditional medicine are a particularly useful source. Materials and methods: In this study, extracts of five different plants that grow in the desert of Saudi Arabia were evaluated to assess their cytotoxicity against the MDA-MB-231 breast cancer cell line. Soxhlet extraction was carried out on the leaves and stems using different solvents. The cytotoxicity of these extracts against MDA-MB-231 breast cancer cells was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The apoptotic cellular morphological changes were observed using inverted and fluorescence microscopes. Results: Our results showed that two of the five different medicinal plants (Rumex vesicarius and Malva parviflora) exhibited strong anticancer activity against the breast cancer cells. Specifically, 2 of the 40 extracts (from the five studied plants) showed promising activity. The chloroform extract of the stem of R. vesicarius (RSV CHCL3) exhibited moderate anticancer activity with a half-maximal inhibitory concentration (IC50) of 230 µg/mL while that of the hexane extract of M. parviflora stems (MPS Hex) was 248 µg/mL. Loss of cell integrity, shrinkage of the cytoplasm, and cell detachment were observed in the extract-treated MDA-MB-231 cells. Conclusion: R. vesicarius and M. parviflora chloroform and n-hexane stem extracts showed significant cytotoxicity against MDA-MB-231 human breast carcinoma cells.

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Medicinal plants used in Jeddah, Saudi Arabia: A gender perspective

Journal of Ethnopharmacology ◽

10.1016/j.jep.2020.112899 ◽

2020 ◽

Vol 257 ◽

pp. 112899 ◽

Cited By ~ 2

Author(s):

Afnan Alqethami ◽

Amal Y. Aldhebiani ◽

Irene Teixidor-Toneu

Keyword(s):

Saudi Arabia ◽

Medicinal Plants ◽

Gender Perspective

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Medicinal plants used in Jeddah, Saudi Arabia: Phytochemical screening

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2020.11.013 ◽

2021 ◽

Vol 28 (1) ◽

pp. 805-812

Author(s):

Afnan Alqethami ◽

Amal Y. Aldhebiani

Keyword(s):

Saudi Arabia ◽

Medicinal Plants ◽

Phytochemical Screening

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Study of the in Vitro Antiplasmodial, Antileishmanial and Antitrypanosomal Activities of Medicinal Plants from Saudi Arabia

Molecules ◽

10.3390/molecules171011379 ◽

2012 ◽

Vol 17 (10) ◽

pp. 11379-11390 ◽

Cited By ~ 27

Author(s):

Nawal M. Al-Musayeib ◽

Ramzi A. Mothana ◽

Shaza Al-Massarani ◽

An Matheeussen ◽

Paul Cos ◽

...

Keyword(s):

Saudi Arabia ◽

Medicinal Plants

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Screening antifungal activities of selected medicinal plants

Journal of Ethnopharmacology ◽

10.1016/s0378-8741(00)00350-0 ◽

2001 ◽

Vol 74 (1) ◽

pp. 89-96 ◽

Cited By ~ 125

Author(s):

Emma Nelly Quiroga ◽

Antonio Rodolfo Sampietro ◽

Marta Amelia Vattuone

Keyword(s):

Medicinal Plants ◽

Antifungal Activities

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Comparative Study of the Antimicrobial Efficacy of Three Medicinal Plants against Dermatophytic Pathogens

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i1030423 ◽

2021 ◽

pp. 32-48

Author(s):

Temitayo Veronica Folorunso ◽

Anthony Kayode Onifade ◽

Kolawole Oladunmoye ◽

Bamidele Juliet Akinyele

Keyword(s):

Medicinal Plants ◽

Jatropha Curcas ◽

Fungal Infections ◽

Dilution Method ◽

Agar Dilution Method ◽

Bixa Orellana ◽

Cross Sectional ◽

Antifungal Activities ◽

Cassia Alata ◽

Ethanol Extracts

Bixa orellana, Jatropha curcas and Cassia alata are three of the prominent plants used for traditional medicine in Nigeria. Dermatophytosis also known as tinea or ringworm is the most frequent superficial fungal infections in Nigeria. Objective: In this Present Study, We Aimed at Comparing The Phytochemical Components and the Antifungal Efficacy of these Medicinal against Selected Dermatophytes. Study Design: Cross Sectional Study among a Particular Population. Place and Duration of Study: Department of Microbiology, Federal University of Technology, Akure, Ondo State. Between March 2019 and September 2019. Methods: The phytochemical contents of the plants were determined and the in-vitro antifungal activities of Bixa orellana, Jatropha curcas and Cassia alata were screened against seven species of Trichophyton (T. ajelloi, T. mentagrophytes, T. rubrum, M.gypseum T. soudenensis, T. tonsurans and T. verrucosum) using agar dilution method. Results: The phytochemical screening revealed the presence of flavonoid, saponin, phenol, steroids, glycoside, phytosteroids, alkaloids, terpenoid, tannin, and cardiac glycoside in various quantities. The findings from our study showed that the ethanol extracts of these medicinal plants have more antifungal activities than other solvents. However, the hexane and ethanol extracts of Jatropha curcas was observed to be significantly higher than other extracts. The zone of inhibition recorded ranges from 22 mm-32 mm and the minimum inhibitory concentration (MIC) of 12.5 mg/ml was recorded. Conclusion: The ethanolic extract of Jatropha curcas showed broad effectiveness against the tested pathogens when compared to other plants and we conclude that the plants antifungal property is concentration dependent. However, we recommend further studies on these plants extracts using a large number of different isolates and solvents.

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Antibacterial and Antifungal Activities of Ethiopian Medicinal Plants: A Systematic Review

Frontiers in Pharmacology ◽

10.3389/fphar.2021.633921 ◽

2021 ◽

Vol 12 ◽

Author(s):

Dereje Nigussie ◽

Gail Davey ◽

Takele Beyene Tufa ◽

Malcolm Brewster ◽

Belete Adefris Legesse ◽

...

Keyword(s):

Medicinal Plants ◽

Plant Species ◽

Meta Analysis ◽

Fungal Species ◽

Antifungal Activities ◽

Traditional Medicines ◽

Antibacterial And Antifungal Activities ◽

Wide Range ◽

Antibacterial And Antifungal

Background: Podoconiosis and lymphatic filariasis are the most common causes of lower limb lymphoedema in the tropics. Many sufferers experience frequent painful episodes of acute bacterial infection. Plant based traditional medicines are used to treat infections in many countries and are culturally established in Ethiopia. Ethiopian medicinal plants found to have antibacterial and antifungal activities were reviewed with the aim of increasing information about the treatment of wound infections in patients with lymphoedema.Methods: This study collates data from published articles on medicinal plants with antibacterial and antifungal activities in Ethiopia. A systematic search of Scopus, EMBASE, PUBMED/MEDLINE and Google Scholar was undertaken. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines were followed. The protocol was registered on PROSPERO with registration number CRD42019127471. All controlled studies of in vitro antibacterial and antifungal activities were considered. All articles containing the descriptors published until June 28, 2019 were included. The outcome was measured as percent inhibition of microbial growth. For quality assessment of individual in vitro studies, OECD guidelines and the WHO-Good Laboratory Practice (GLP) handbook were used.Results: Seventy-nine studies met the inclusion criteria. A total of 150 plant species and three compounds had been tested against 42 species of bacteria, while 43 plant species had been tested against 22 species of fungus.Conclusion: Materials derived from several Ethiopian medicinal plants have been shown to have promising activity against a variety of bacteria and fungi. Those derived from Azadiractha indica A. Juss. and Lawsonia inerms L. are the most extensively studied against a wide range of gram-negative and positive bacteria, and fungal species.

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