Lippia alba (Mill.) N.E. Essential Oil Interfere with Aminoglycosides Effect Against Staphylococcus aureus

In vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL−1 when LA1EO and citral were used. Potential inhibition (100%) of S. aureus biofilm formation at the concentration of 0.5 mg mL−1 of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL−1, 2 mg mL−1, 2 mg mL−1, and 0.5 mg mL−1 for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of L. alba EOs against S. aureus, a species of recognized clinical interest.

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Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

Phytothérapie ◽

10.3166/phyto-2019-0150 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S155-S163 ◽

Cited By ~ 2

Author(s):

S. Mehalaine ◽

O. Belfadel ◽

T. Menasria ◽

A. Messaili

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Rosmarinus Officinalis ◽

Salvia Officinalis ◽

Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

Zeitschrift für Naturforschung C ◽

10.1515/znc-2020-0051 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Nursenem Karaca ◽

Görkem Şener ◽

Betül Demirci ◽

Fatih Demirci

Keyword(s):

Staphylococcus Aureus ◽

Essential Oil ◽

Antibacterial Effect ◽

Synergistic Effects ◽

Pharmaceutical Formulations ◽

Human Pathogens ◽

Broth Microdilution ◽

Antibacterial Effects ◽

Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

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Fungicidal effect of Lippia alba essential oil on a white-rot fungus

Maderas Ciencia y tecnología ◽

10.4067/s0718-221x2015005000003 ◽

2015 ◽

pp. 0-0

Author(s):

K.V.N Geromini ◽

F.B Roratto ◽

F.G Ferreira ◽

J Camilotti ◽

T.M.A Vidigal ◽

...

Keyword(s):

Essential Oil ◽

White Rot ◽

White Rot Fungus ◽

Fungicidal Effect ◽

Lippia Alba

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Lippia alba essential oil improves water quality during transport and accelerates the recovery of Potamotrygon wallacei from the transport-induced stress

Aquaculture ◽

10.1016/j.aquaculture.2021.737176 ◽

2021 ◽

pp. 737176

Author(s):

Karine Ariotti ◽

Jaydione L. Marcon ◽

Isabela A. Finamor ◽

Caroline A. Bressan ◽

Cristiano L. De Lima ◽

...

Keyword(s):

Water Quality ◽

Essential Oil ◽

Lippia Alba ◽

Induced Stress

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Essential Oil Composition and Antioxidant Capacity of Carum copticum and its Antibacterial Effect on Staphylococcus aureus , Enterococcus faecalis and Escherichia coli O157:H7

Journal of Food Processing and Preservation ◽

10.1111/jfpp.12938 ◽

2016 ◽

Vol 41 (3) ◽

pp. e12938 ◽

Cited By ~ 9

Author(s):

Maryam Mahmoudzadeh ◽

Hedayat Hosseini ◽

Farzaneh Shahraz ◽

Afshin Akhondzadeh-Basti ◽

Amin Mousavi Khaneghah ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Essential Oil ◽

Antioxidant Capacity ◽

Enterococcus Faecalis ◽

Antibacterial Effect ◽

Essential Oil Composition ◽

Escherichia Coli O157 ◽

Oil Composition ◽

Carum Copticum

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Modulatory Effect of Lippia alba Essential Oil on the Activity of Clinically Used Antimicrobial Agents on Salmonella typhi and Shigella dysenteriae Biofilm

Scientia Pharmaceutica ◽

10.3390/scipharm86040052 ◽

2018 ◽

Vol 86 (4) ◽

pp. 52

Author(s):

Andressa Batista ◽

Hilania Dodou ◽

Matheus Rodrigues ◽

Pedro Pereira ◽

Gleilton Sales ◽

...

Keyword(s):

Essential Oil ◽

Antimicrobial Agent ◽

Cell Viability ◽

Antimicrobial Agents ◽

Salmonella Typhi ◽

Shigella Dysenteriae ◽

Lippia Alba ◽

Microdilution Method ◽

Pharmacological Potential ◽

Main Components

The essential oil obtained from the leaves of Lippia alba (Mill.) N.E. Brown (Verbenaceae) has shown great pharmacological potential as an analgesic, antispasmodic, and antimicrobial agent. The aim of this study was to evaluate the modulatory effect of Lippia alba essential oil (LaEO I) on the activity of clinically used antimicrobial agents on Salmonella enterica serovar Typhi (Salmonella typhi) and Shigella dysenteriae biofilms. The Minimum Inhibitory Concentration of LaEO I (MICLaEO I) was determined by the microdilution method, and the effect of LaEO I on the activity of clinically used antimicrobials was assessed by the Checkboard method. The values obtained from MICLaEO I and ciprofloxacin were used to evaluate the effect of time of exposure on cell viability. LaEO I main components were geranial (34.2%), neral (25.9%), and myrcene (12.5%). The MICLaEO I was 1 mg/mL for both strains. LaEO I positively modulated the action of ciprofloxacin, cefepime, and ceftriaxone. After the first hour of treatment with MICLaEO I, the cell viability of the strains showed a 5 log10 CFU/mL reduction, and the LaEO I-CIP association was able to inhibit growth during the first 6 h of the test. Regarding the anti-biofilm activity, MICLaEO I was able to reduce the biofilm mass of Salmonella typhi by 61.2% and of Shigella dysenteriae by 38.9%. MICLaEO I was not able to eradicate the preformed biofilm; however, there was a reduction in the biofilm microbial viability. LaEO I has the potential to be used as an antimicrobial agent and interferes with biofilm formation; also, it is able to reduce cell viability in preformed biofilm and synergistically modulate the activity of ciprofloxacin.

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