Antioxidant Activity, Free Radical Scavenging Potential and Chemical Composition ofLitsea cubebaEssential Oil

2012 ◽  
Vol 15 (1) ◽  
pp. 134-143 ◽  
Author(s):  
Ying Wang ◽  
Zi-Tao Jiang ◽  
Rong Li
2011 ◽  
Vol 183-185 ◽  
pp. 854-858 ◽  
Author(s):  
Chuan Ling Si ◽  
Pan Pan Qin ◽  
Yi Yuan Lu ◽  
Lei Wu ◽  
Hai Hua Wang ◽  
...  

Elaeagnus angustifolia L., a hardwood species in the family of Elaeagnaceae, has long been used as part of traditional medicines to treat various diseases. However, limited study is available on the chemical composition and antioxidant activity of E. angustifolia bark. In this study, we undertook a phytochemical investigation of E. angustifolia bark by gas chromatographic and mass spectrometric (GC-MS) analysis. And antioxidant activity of the crude and following soluble fractions of E. angustifolia bark was evaluated by DPPH free radical scavenging assay. GC-MS investigation of 95% EtOH crude extracts from E. angustifolia bark detected 40 compounds, and DPPH free radical scavenging assay indicated that the bark EtOAc and BuOH soluble fractions exhibited significant antioxidant potential (IC50 values of 12.1 and 12.4 µg/ml, respectively) comparing with α-tocopherol and BHT (IC50 values 12.3 and 13.8 µg/ml, respectively), which were used as positive controls.


2012 ◽  
Vol 554-556 ◽  
pp. 1125-1128 ◽  
Author(s):  
De La Zhang ◽  
Yu Hong Feng ◽  
Zhen Yi Liang ◽  
Qiang Lin ◽  
Jing Xu

The chemical composition of hydrodistilled oil from the fruit residue of Ficus altissima was analyzed for the first time by GC/GC–MS. As a result, a total of seventeen compounds were identified, representing all of the oil. Aliphatic hydrocarbons, esters and alkylenes were found to compose three major chemotype accounted for 64.25%, 26.00% and 9.75% of the oil, respectively. The main components was demonstrated to be dibutyl phthalate(18.14%), n-docosane(7.94%), diisooctyl phthalate(7.86%), n-heptadecane(7.70%), n-tricosane(7.68%), phytane(7.19%). Antioxidant activity of the oil was assessed by the free radical scavenging (DPPH). The study offers theoretic basis for pharmaceutical utilization of the landscape plant F. altissima.


2013 ◽  
Vol 65 (1) ◽  
pp. 321-329 ◽  
Author(s):  
Abdulhamid Giweli ◽  
Ana Dzamic ◽  
Marina Sokovic ◽  
M.S. Ristic ◽  
P. Janackovic ◽  
...  

The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), ?-pinene (7.38%), myrcene (7.38%), ?-pinene (5.15%), ?-caryophyllene (4.13%) and ?-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.


2005 ◽  
Vol 93 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Malgorzata Nogala-Kalucka ◽  
Jozef Korczak ◽  
Małgorzata Dratwia ◽  
Eleonora Lampart-Szczapa ◽  
Aleksander Siger ◽  
...  

2014 ◽  
Vol 1081 ◽  
pp. 110-114 ◽  
Author(s):  
Zhen Zhu ◽  
Hua Yin ◽  
Yan An

This research adopts the pancreatin hydrolysis of silk fibroin active peptide, evaluate the antioxidant activity of hydrolysates. In the process of hydrolysis of silk fibroin, by measuring the amino nitrogen content of neutral formaldehyde titration method. Find the amino nitrogen content gradually stabilized at around 0.37g/L, and superoxide free radical scavenging rate changing with time fluctuation trend, superoxide free radical scavenging rate to a maximum of 65.03% at 220min.The use of silk fibroin hydrolysis process optimization,reaction time 160min, enzyme concentration4% , substrate concentration 20mg/ml, pH 8, temperature 38°C. The hydrolysis process under the hydrolysate on superoxide radical scavenging rate of 72.73%. The scavenging rate of hydroxyl radical is 47.24%. Red blood cell hemolysis induced by H2O2 inhibition rate was 24.30%.


Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents


2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.


2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>


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