Nickel Nanoparticles Originated from Cressa Leaf Extract in the Preparation of a Novel Melem@Ni-HPA Photocatalyst for the Synthesis of Some Chromenes and a Preliminary MTT Assay on the Anticancer Activity of the Nanocomposite

2021 ◽  
pp. 1-20
Author(s):  
Zhenli Wei ◽  
Sedighe Abbaspour ◽  
Reza Tayebee
Keyword(s):  
Anticancer Activity ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Nickel Nanoparticles

Enhanced Anticancer Activity and Apoptosis Effect of Bioactive Compound Quercetin Extracted from Ocimum sanctum Leaves

2020 ◽  
Vol 10 ◽  
Author(s):  
Amutha Santhanam ◽  
Naveen Kumar Chandrasekharan ◽  
Rajangam Ilangovan
Keyword(s):  
Cell Cycle ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Leaf Extract ◽  
Nickel Nanoparticles ◽  
Bioactive Compound ◽  
Cell Cycle Analysis ◽  
Ocimum Sanctum ◽  
Sem Images ◽  
Ft Ir

Background: The occurrence of Cancer results in cellular changes that causes the uncontrolled growth and division of cells. Apoptosis removes cells during development and eliminates the potentially cancerous cells. The bioactive compounds present in the herbal plant shows cytotoxic activity that result in apoptosis. The traditional herbal plants are used world-wide both in allopathy and other traditional ways. Objective: The main objective of this study is to extract the bioactive compound Quercetin from the medicinally significant plant Ocimum sanctum and also to develop nanomedicine as Qu-PEG-NiGs. Materials and Methods: Leaf extract of the medicinally significant plant Ocimum sanctum (O. sanctum) has been used for the synthesis of nickel nanoparticles (NiGs) and extraction of quercetin (Qu). The ethanolic extract of Ocimum sanctum is added to 1 mM Nickel Nitrate (Ni(NO3)2) and stirred for 3 hrs at RT and dried at 60°C for 3hrs and calcinated at 400°C for 2hrs and characterized using Uv-Vis Spectrophotometer, FT-IR, SEM, DLS and Zeta potential. The Quercetin is isolated from Ocimum sanctum leaf extract using the reflux condenser method. The bio-polymer is being PEG-coated over NiGs and Quercetin is loaded into it. The apoptosis activity using MCF-7 cells is performed with Qu-PEG-NiGs. The purity of Quercetin is characterized using HPLC. In order to analyse apoptosis efficiency, MTT assay, Reactive Oxygen Species (ROS), Cell cycle analysis has been performed. Results: The NiGs absorption spectrum gives a peak at 408nm. The FT-IR confirms the presence of particular functional groups shifting from the compound NiGs and then coated with PEG-Qu-NiGs. The SEM images show the size of NiGs ranging from 27.3 nm to 40.4 nm with varied morphology such as hexagonal and other irregular shapes. The presence of Quercetin extracted from the leaf powder is approximately 1.5 mg/g. The ROS results show the Qu-PEG-NiGs induced efficiency of the apoptosis, while the increased concentrations promote ROS and lead to activation of the apoptosis. The cell cycle analysis has shown the cytotoxic effect. Conclusion: PEG-coated nickel nanoparticles can be used as a promising chemotherapeutic agent against MCF7 breast cancer cells. It is the evidence to further studies for evaluating Qu-PEG-NiGs anticancer activity on different types of cancer cells.

Bis (Isothiocyanatomethyl) Benzene, A Plant Derived Anti-Neoplastic Compound: Purified from Moringa Oleifera Leaf Extract

2019 ◽  
Vol 19 (5) ◽  
pp. 677-686 ◽  
Author(s):  
Samrat Paul ◽  
Piyali Basak ◽  
Namrata Maity ◽  
Chayan Guha ◽  
Nandan Kumar Jana
Keyword(s):  
Anticancer Activity ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Drug Formulation ◽  
Indigenous Species ◽  
Cancer Cell Growth ◽  
Apoptotic Pathway ◽  
Dimeric Form ◽  
Mcf 7

Background: Moringa oleifera lam, commonly known as “Sajina”, is an indigenous species to India. In our folk medicine, it is used for the treatment of Canker (cancer). The Moringa oleifera leaf extract contains many phyto-compounds, with some being anti-neoplastic in nature. Objective: Our preliminary study showed that the leaf extract significantly kills cancer cells compared to normal cells. On searching for the new phyto-compound, Bis-isothiocyanatomethyl) benzene was purified and isolated. Methods: The sequential process of fractional distillation, column chromatography, followed by TLC and HPLC is performed for purification. Every fraction from each step was tested on HeLa cell line for evaluating the presence of the phyto-compound. Results and Conclusion: FTIR peak analysis of a single phyto-compound shows the presence of thiocyanate group, aromatic carbon group. 1H & 13C NMR peak analysis along with High-resolution mass spectroscopy (HRMS) calculation confirm the chemical structure with IUPAC name [Bis (Isothiocyanatomethyl) benzene]. Previously, Isothiocyanatomethyl- benzene solely or in conjugation with sugar molecule has been reported, but its dimeric form in nature hasnot yet been published anywhere. It shows anticancer activity by retarding cancer cell growth & inhibits carcinogenesis on HeLa, MCF-7, and MDA-MB-231 cell lines by caspase 3 apoptotic pathway and showed comparatively less cytotoxicity to PBMC cell. It shows anticancer activity almost the same as the market available drug Cis-Platin. Therefore, further extrapolating its activity with different concentrations may result in its use as a drug formulation for the treatment of cancer.

scholarly journals Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity

Crystals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 571
Author(s):  
Ahmed Gaber ◽  
Walaa F. Alsanie ◽  
Majid Alhomrani ◽  
Abdulhakeem S. Alamri ◽  
Ibrahim M. El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carboxylic Acid ◽  
Anticancer Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Anticancer Effect ◽  
Reference Compound ◽  
Anticancer Effects ◽  
Mcf 7

This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b, 7c, 8a, 8b, and 8c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b, 7c, 8a, 8b, and 8c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.

Synthesis and anticancer activity of paclitaxel−coumarin conjugate

2021 ◽  
Vol 18 ◽  
Author(s):  
Zurong Song ◽  
Qin Lu ◽  
Ali Tao ◽  
Tianchen Wu
Keyword(s):  
Flow Cytometry ◽  
Synergistic Effect ◽  
Anticancer Activity ◽  
Mtt Assay ◽  
Scratch Test ◽  
The Novel ◽  
Effective Strategy ◽  
Test Results ◽  
Antibacterial Effects ◽  
Anti Tumor Activity

Background: Paclitaxel, a natural diterpenoid compound, has anti-tumor effect by acting on tubulin; coumarin, another kind of natural product, has anti-tumor, antibacterial effects and so on. Moreover coumarin has fluorescence. Objective: Multi target to combat tumor is an effective strategy in drug design. Therefore, combination of paclitaxel with other acticive moleculer to explore the novel lead with multi-functons is in demand. Materials and Methods: To synthsize paclitaxel-coumarin conjugate via click chemistry and to investigate anticancer activity by MTT assay and the scratch test. Results and Discussion: The results of MTT assay showed that compared with paclitaxel, the anti-tumor activity of the conjugate was significantly improved. The results of flow cytometry showed that the conjugate had stronger ability to induce apoptosis. The scratch test results showed that the conjugate had better anti- metastasis ability than paclitaxel. Conclusion: These findings indicated that paclitaxel and coumarin had synergistic effect, which provided a new idea for the development of paclitaxel monitored by fluorescence.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF CYCLOHEXANE-1,3-DIONE DERIVATIVES AND THEIR IN SILICO AND IN VITRO STUDIES ON ANTIMICROBIAL AND BREAST CANCER ACTIVITY

2019 ◽  
pp. 311-320 ◽  
Author(s):  
RAJA CHINNAMANAYAKAR ◽  
EZHILARASI MR ◽  
PRABHA B ◽  
KULANDHAIVEL M
Keyword(s):  
Breast Cancer ◽  
Antimicrobial Activity ◽  
Anticancer Activity ◽  
Mtt Assay ◽  
In Silico ◽  
Biologically Active ◽  
Diffusion Method ◽  
Breast Adenocarcinoma ◽  
In Silico Docking

Objective: The objective of this study was to evaluate in silico and in vitro anticancer activity for synthesized cyclohexane-1,3-dione derivatives. Methods: The new series of cyclohexane-1,3-dione derivatives were synthesized based on the Michael addition reaction. Further, the structures of the synthesized compounds were confirmed by Fourier-transform infrared spectroscopy, 1H nuclear magnetic resonance (NMR), and 13C NMR spectral data. Then, the in silico molecular docking studies were carried out using AutoDock tool version 1.5.6 and AutoDock version 4.2.5.1 docking program. The antimicrobial activity was carried out using the agar disk diffusion method, and the in vitro anticancer activity was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for the synthesized compound. Results: In silico docking study, compound 5c showed good binding score and binding interactions with selected bacterial proteins and breast cancer protein. Further, compound (5a-5h) was tested for their antimicrobial activity and compound 5c was only tested for anticancer activity (human breast adenocarcinoma 3,4-methylenedioxyamphetamine-MB-231 cell line). Compound 5c was found to be the most active one of all the tested compounds. In the MTT assay compound, 5c showed the LC50 value of 10.31±0.003 μg/ml. In antimicrobial activity, the minimum inhibitory concentration of compound 5c is 2.5 mg/ml. Conclusion: An efficient synthesis of biologically active cyclohexane-1, 3-dione derivatives has been developed.

scholarly journals Potential anticancer activity of biogenic silver nanoparticles using leaf extract of Rhynchosia suaveolens: an insight into the mechanism

2018 ◽  
Vol 46 (sup1) ◽  
pp. 104-114 ◽  
Author(s):  
Murali Satyanarayana Bethu ◽  
Vasudeva Reddy Netala ◽  
Latha Domdi ◽  
Vijaya Tartte ◽  
Venkateswara Rao Janapala
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Leaf Extract ◽  
Biogenic Silver Nanoparticles ◽  
Insight Into

scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF PLUMBAGO ZEYLANICA LINN LEAF EXTRACT

2011 ◽  
Vol 1 (2) ◽  
Author(s):  
Sachin Hiradeve ◽  
Kishor Danao ◽  
Vijay Kharabe ◽  
Bibhilesh Mendhe
Keyword(s):  
Anticancer Activity ◽  
Leaf Extract ◽  
Plumbago Zeylanica

scholarly journals IMMUNOMODULATORY, ANTICANCEROUS & ANTIOXIDANTACTIVITIES OF CYCLEA PELTATA (LAM.) HOOK.F. & THOMSON

2019 ◽  
pp. 40-46
Author(s):  
SONY JAYARAMAN ◽  
E. JAYADEVI VARIYAR
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Mtt Assay ◽  
Lymphocyte Proliferation ◽  
Immunomodulatory Activity ◽  
Macrophage Cell ◽  
Nitrite Production ◽  
Total Antioxidant

Introduction: Cyclea peltata (Lam.) Hook.f. & Thomson is an important plant listed in Ayurveda. The plants are used in digestive, diuretic and inflammatory disorders. Aim: The present study was to study the immunomodulatory, anticancerous and antioxidative properties of the fraction isolated from Cyclea peltata. Methods: The immunomodulatory activity was evaluated in lymphocytes and THP-1 macrophage cell lines. The anticancer activity of the fraction was determined by MTT assay. The antioxidant activity was evaluated by DPPH and total antioxidant assays. Results: The fraction showed the presence of flavanoids which induced lymphocyte proliferation and also could induce nitrite production in THP-1 cell lines at 1mg/ml. It also exhibited good anticancerous activity at 100 μg/ml after 48h of incubation.  The antioxidant activity was less at this concentration and was found to be equivalent to 79± 0.03 μg/ml of ascorbic acid. Conclusion: The fraction containing flavonoid, isolated from Cyclea peltata have shown to have good immunomodulatory, antioxidant and anticancerous property.

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