Headspace Analyses of Leaf and Flower ofCitrus limetta(Lemon),Citrus maxima(Pomelo),Citrus sinensis(Orange), andCitrus medica(Cedrum) for Volatile Compounds by CombiPAL System Technique

Objective: Total investigation of the macro-and micromorphological features of Citrus maxima (Burm.) Merrill. In addition, DNA fingerprinting in this study was to estimate genetic polymorphism and relationships with another citrus viz: Citrus paradise and Citrus sinensis based on RAPD and ISSR markers.Methods: For the establishment of macro-and micromorphological investigation and genetic estimation, this study presents a total investigation of the macro-and micromorphological features of the roots, stems, leaves, flowers, fruits and seeds of the plants through microscopical investigation of the prepared entire, transverse sections and powdered forms of different organs under study. Furthermore, the DNA of the three plants was extracted from leaf samples and two types of DNA markers RAPD and ISSR were assayed.Results: Macro-and micromorphological features of different organs were investigated. On the other hand Genetic polymorphism and relationships among Citrus maxima, Citrus paradisi and Citrus sinensis was revealed. The highest genetic similarity was between Citrus maxima and Citrus paradisi while the lowest similarity was between Citrus paradisi and Citrus sinensis as revealed by RAPD, ISSR and combined data.Conclusion: For the present study, macro and micro-morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters for authentication of Citrus maxima (Burm.) Merrill.

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First report of Xanthomonas citri Subsp. Citri causing citrus canker on grape fruit (Citrus paradisi), washington naval (Citrus sinensis), kaghzi limon (Citrus aurantifolia Swingle), lemon (Citrus lim

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/21.9701 ◽

2021 ◽

Vol 58 (04) ◽

pp. 1373-1377

Author(s):

Muhammad Shah Nawaz ul Rehman

Keyword(s):

Citrus Sinensis ◽

Sequence Similarity ◽

Citrus Canker ◽

Citrus Limon ◽

Evolutionary Analysis ◽

Citrus Paradisi ◽

Xanthomonas Citri ◽

Citrus Aurantifolia ◽

Grape Fruit ◽

Citrus Maxima

Citrus fruit production is largely affected by different bacterial and fungal pathogens. In Pakistan bacterial diseases like citrus bacterial canker (CBC) pose severe risk to citrus economy. Diagnoses of such diseases could be helpful to avoid the epidemics in nurseries or orchids. In 2011-12, citrus canker symptoms i.e., callus-like outgrowths on leaves and fruits of grape fruit (Citrus paradisi), Washington naval (Citrus sinensis), Kaghzi Limon (Citrus aurantifolia swingle), lemon (Citrus Limon) and pomelo (Citrus maxima) were noticed in Sargodha district of Punjab, Pakistan. Bacteria i.e., yellow mucoid, Xanthomonas- like isolates, were isolated from these lesions. Bacteria isolated from these lesions were cultured and total DNA was isolated. A diagnostic fragment of 581 bp based on rpf genes of Xanthomonas citri pv. citri was amplified, cloned and completely sequenced. BLAST and evolutionary analysis revealed that these isolates show 100% sequence similarity and group with Xanthomonas citri subsp. citri from Argentina (CP023285) and Reunion (CP018858), (CP018854). To our knowledge, this is the first formal report of X. campestris pv. citri pathotypes A on Citrus paradise, Citrus sinensis, Citrus maxima, Citrus Limon and Citrus aurantifolia swingle in Pakistan

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Antioxidant activities and glycemic indices of ice creams enriched with orange ( Citrus sinensis ) and shaddock ( Citrus maxima ) peels and effects on rat lipid profiles

Journal of Food Biochemistry ◽

10.1111/jfbc.13813 ◽

2021 ◽

Author(s):

Ayokunle Olubode Ademosun ◽

Ganiyu Oboh ◽

Olufunke Florence Ajeigbe

Keyword(s):

Citrus Sinensis ◽

Antioxidant Activities ◽

Lipid Profiles ◽

Glycemic Indices ◽

Citrus Maxima ◽

Ice Creams

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HUBUNGAN KEKERABATAN FENETIK 7 SPESIES JERUK DI DATARAN TINGGI BENER MERIAH

Jurnal Jeumpa ◽

10.33059/jj.v8i2.4398 ◽

2021 ◽

Vol 8 (2) ◽

pp. 545-553

Author(s):

Aulia Agustina ◽

Hasanuddin Hasanuddin

Keyword(s):

Citrus Sinensis ◽

Similarity Index ◽

Morphological Characteristics ◽

Morphological Characterization ◽

Dissimilarity Index ◽

Citrus Aurantifolia ◽

Citrus Maxima ◽

Phenetic Relationship ◽

Relationship Of

The study about “Phenetic Relationship of 7 Species of Orange Plants in BenerMeriah Highlands” was inducted back in August until September 2017. The aim of this study is to describe the morphological characterization of 7 species of orange plants in BenerMeriah highlands and to understand the phenetic relationship of 7 species of oranges plant in BenerMeriah highlands. The method used in this study was observation with the type of mixture between qualitative and quantitative. The subjects of this study are 7 species of orange plants, namely: a) Citrus reticulata; b) Citrus amblycarpa; c) Citrus hystrix; d) Citrus nobilis; e) Citrus maxima; f) Citrus sinensis; and g) Citrus aurantifolia. The parameters of this study were the morphological characteristic of stems, leaves, fruits and flowers. The collected data were analyzed descriptively, where the similarities were measured using Similarity Index (SI) and the dissimilarities were measured using Dissimilarity Index (DI) to classify the total of 7 species of orange plants using cluster analysis. According to the morphological characteristics, it could be concluded that there are several differences and similarities among the 7 species of orange plants found in BenerMeriah Regency. The combination of Citrus hystrix with Citrus aurantifolia have the closest phenetic relationship, which have 66 of SI. The other combinations that also have close phenetic relationship are Citrus hystrix with Citrus maxima and Citrus nobilis with Citrus sinensis. The pheneticrelationship that have the highest DI is the combination of Citrus amblycarpa with Citrus maxima, which have 88 of DI

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Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis

10.1101/2021.11.27.470207 ◽

2021 ◽

Author(s):

Valerie Amber Buijs ◽

Johannes Z. Groenewald ◽

Sajeet Haridas ◽

Kurt LaButti ◽

Anna Lipzen ◽

...

Keyword(s):

Citrus Sinensis ◽

Black Spot ◽

Economic Losses ◽

Citrus Limon ◽

Carbohydrate Active Enzymes ◽

Genomic Comparison ◽

Citrus Black Spot ◽

Genomic Approach ◽

Citrus Maxima ◽

Fungal Genus

Members of the fungal genus Phyllosticta can colonize a variety of plant hosts, including several Citrus species such as Citrus sinensis (orange), Citrus limon (lemon), and Citrus maxima (pomelo). Some Phyllosticta species have the capacity to cause disease, such as Citrus Black Spot, while others have only been observed as endophytes. Thus far, genomic differences underlying lifestyle adaptations of Phyllosticta species have not yet been studied. Furthermore, the lifestyle of Phyllosticta citrichinaensis is ambiguous, as it has been described as a weak pathogen but Kochs postulates may not have been established and the presence of this species was never reported to cause any crop or economic losses. Here, we examined the genomic differences between pathogenic and endophytic Phyllosticta spp. colonizing Citrus and specifically aimed to elucidate the lifestyle of Phyllosticta citrichinaensis. We found several genomic differences between species of different lifestyles, including groups of genes that were only present in pathogens or endophytes. We also observed that species, based on their carbohydrate active enzymes, group independent of their phylogenetic association, and this clustering correlated with trophy prediction. Phyllosticta citrichinaensis shows an intermediate lifestyle, sharing genomic and phenotypic attributes of both pathogens and endophytes. We thus present the first genomic comparison of multiple citrus-colonizing pathogens and endophytes of the genus Phyllosticta, and therefore provide the basis for further comparative studies into the lifestyle adaptations within this genus.

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