Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity

2021 ◽  
pp. 1-11
Author(s):  
Ling Sun ◽  
Jing-Jing Lu ◽  
Bi-Xu Wang ◽  
Tong Sun ◽  
Bing-Qi Zhu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Tetrastigma Hemsleyanum ◽  
Optimum Extraction

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals Studied on the extraction, response surface analysis and antioxidant activity of flavonoids in the Momordia cochinchinensis seeds

2018 ◽  
Vol 238 ◽  
pp. 04004
Author(s):  
Junyu Jiang ◽  
Aiping Fei ◽  
Yong Wang ◽  
Zhigang Fang ◽  
Yingxue Teng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Surface Analysis ◽  
Ethanol Concentration ◽  
Response Surface Analysis ◽  
Extraction Time ◽  
Average Yield ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

This text was mainly studied the extraction flavonoids technology from Momordia cochinchinensis seeds. The optimum extraction condition was as follows: when the ratio of liquid to solid was 20:1, the ethanol concentration was 90% and the extraction time was 2h. Under this condition, the actual average yield of flavonoids could reach 0.363%.

scholarly journals Effect of extraction temperature and time on the extraction of phenolic compounds and antioxidant capacity of Phaleria macrocarpa fruit

2018 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Peng Yee Lim ◽  
Sook Fun Pang ◽  
Mashitah Mohd Yusoff ◽  
Jolius Gimbun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Pearson Correlation Coefficient ◽  
Flavonoid Content ◽  
Phaleria Macrocarpa ◽  
Optimum Extraction

Phaleria macrocarpa (ver. name: ‘mahkota dewa’) is a plant which has many medically useful antioxidant activities (Anggraini & Lewandowsky, 2015). The polyphenols responsible for this antioxidant activity has to be extracted before it can be routinely used (Shwter et al., 2016). This study investigates the extraction of polyphenols from P. macrocarpa fruits and its antioxidant activity (DPPH-RSA) under influence of extraction time and temperature. By employing maceration technique, the P. macrocarpa fruits extract showed the maximum total phenolic content (TPC), total flavonoid content (TFC) and DPPH-RSA with value of 69.5 mg QE/g DW, 183.2 mg GA/g DW and 171. 8 mg BHA/g DW, respectively at  optimum extraction conditions of 60 min and 80 ºC (Fig. 1). Excellent and positive Pearson correlation coefficient with R2 > 0.91 between the TPC, TFC and antioxidant activities was observed.

scholarly journals Seasonal Variation of Biosynthetic Path Selectivity of Flavonoids, and Content and Antioxidant Activity of Metabolites in Tetrastigma Hemsleyanum Diels Et Gilg

2021 ◽  
Author(s):  
YanShou Shi ◽  
Li Yang ◽  
MinFen Yu ◽  
ZhaoHui Li ◽  
ZhiJun Ke ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Seasonal Variation ◽  
Seasonal Variations ◽  
Reaction Path ◽  
Sunshine Duration ◽  
Reaction Network ◽  
Total Content ◽  
Tetrastigma Hemsleyanum

Abstract This work investigated the seasonal variations in biosynthetic path selectivity of nine individual flavonoids, and content and antioxidant activity of three major metabolites in Tetrastigma hemsleyanum. The results revealed that under conditions of precipitation (2.0~6.6 mm), temperature (17.5~24.1°C), humidity (67.3~80.2%) and sunshine duration (3.4~5.8 h) in April and May, the total content of flavonoids reached higher levels between 282 and 394.5 μg/g; in the second half of April with the highest accumulation of flavonoids, the production selectivity (PS) of isoorientin, orientin, rutin, isoquercitin, kaempferol-3-0-rutinoside, astragalin, quercetin, apigenin and kaempferol were 0.30, 0.06, 0.07, 0.07, 0.00, 0.04, 0.38, 0.05 and 0.03, respectively; also according to the reaction network consisting of three main pathways for flavonoids syntheses in T. hemsleyanum, the selectivity of reaction Path II to synthesize quercetin, isoquercitin and rutin in April, May and June fluctuated between 0.52 and 0.66, much higher than that of reaction Path I or Path III.

scholarly journals Efek Durasi Waktu Ekstraksi dan Fraksinasi terhadap Aktivitas Antioksidan Daun Bakau Api-Api Putih (Avicennia marina)

2020 ◽  
Vol 15 (2) ◽  
pp. 99
Author(s):  
Safrina Dyah Hardiningtyas ◽  
Sri Purwaningsih ◽  
Ekowati Handharyani
Keyword(s):  
Antioxidant Activity ◽  
Synergistic Effect ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Crude Extract ◽  
Day Treatment ◽  
Avicennia Marina ◽  
Natural Antioxidant ◽  
Active Compounds ◽  
Optimum Extraction

Api-api putih (Avicennia marina) merupakan salah satu jenis bakau yang melimpah di Indonesia dan dapat dimanfaatkan sebagai sumber antioksidan alami. Penelitian ini bertujuan untuk menentukan pengaruh waktu ekstraksi dan fraksinasi daun A. marina terhadap aktivitas antioksidannya. Serbuk daun A. marina dimaserasi dengan pelarut etil asetat pada durasi waktu ekstraksi selama 1, 2, 3, dan 4 hari. Sementara itu, fraksinasi ekstrak etil asetat dilakukan dengan kolom kromatografi secara isokratik. Aktivitas antioksidan dari ekstrak kasar dan fraksi-fraksi dianalisis dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Hasil analisis menunjukkan perlakuan selama satu hari merupakan durasi waktu ekstraksi terbaik untuk mendapatkan rendemen ekstrak (1,65±0,01 %) dengan aktivitas antioksidan tertinggi (IC50 186,67±2,82 ppm). Eluen pengembang terbaik untuk fraksinasi ekstrak etil asetat adalah campuran eluen etil asetat: n-heksana (3:7), yang menghasilkan delapan fraksi. Namun, fraksinasi menurunkan aktivitas antioksidan pada fraksi-fraksi, dibandingkan dengan ekstraknya. Hal ini diduga disebabkan oleh efek sinergis antar senyawa dalam ekstrak. Penelitian lebih lanjut dapat dilakukan untuk isolasi senyawa aktif yang terdapat ekstrak etil asetat daun bakau A. marina. ABSTRACTGrey mangrove (Avicenia marina) is one of the abundant types of mangrove in Indonesia, which can be used as a natural antioxidant source. This study aimed to determine the effect of extraction duration and fractionation on the antioxidant activity of A. Marina leaves. A. marina leaves powder was macerated by ethyl acetate solvent for 1, 2, 3, and 4 days. Meanwhile, fractionation was carried out using column chromatography with an isocratic method. The antioxidant activity of crude extracts and fractions was analyzed by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The result showed that one-day treatment was the optimum extraction duration to obtain the extract’s yield (1.65±0.01%) with the highest antioxidant activity (IC50 186.67±2.82 ppm). The best eluent in fractionation was a mixture of ethyl acetate: n-hexane (3:7) eluents, which produced eight fractions. However, fractionation reduced the antioxidant activity of fractions, compared to the crude extract. This might have happened because of the synergistic effect among compounds in the extract. Further research can be carried out to isolate the active compounds in the A. marina leaves extract.

scholarly journals Antioxidant Activity In Vitro Guided Screening and Identification of Flavonoids Antioxidants in the Extract from Tetrastigma hemsleyanum Diels et Gilg

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Li Ding ◽  
Xiaomin Zhang ◽  
Jiajia Zhang
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Free Radical ◽  
High Performance ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Tetrastigma Hemsleyanum ◽  
High Antioxidant Activity ◽  
Radical Scavenging Ability

This study aimed to investigate the extract with high antioxidant activity of Tetrastigma hemsleyanum Diels et Gilg and identify the antioxidant components in vitro. α, α-Diphenyl-β-picrylhydrazyl (DPPH) radical assay, Trolox equivalent antioxidant capacity (TEAC) assay, ferric reducing antioxidant power (FRAP), and hydroxyl radical scavenging method were used to screen the extract with high antioxidant activity. The antioxidant capacity of the extracts was evaluated by the free radical scavenging ability of DPPH. The ability of extracts to scavenge 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical was evaluated by TEAC assay. The FRAP method was used to evaluate the ability of extracts to reduce Fe3+. The ability to scavenge hydroxyl radicals produced by the interaction of hydrogen peroxide and Fe2+ was measured by monitoring the change in the absorbance of the reaction mixture at 536 nm. Then, high-performance liquid chromatography-DPPH (HPLC-DPPH) and HPLC-hydroxyl radical scavenging methods were used to screen the antioxidant components in the extract. The molecular weight of the above antioxidant components was investigated using the qualitative analytical method of high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF LC/MS). Based on the concentrations of the samples (0.2–4 mg/mL), the DPPH free radical scavenging ability, ABTS+ free radical scavenging ability, hydroxyl free radical scavenging ability, and Fe3+ reducing ability of the ethyl acetate extract (EAE) were stronger than that of the crude extract (CE), petroleum ether extract (PEE), and n-butanol extract (BE). The EAE has higher antioxidant activity than CE, PEE, and BE. Six antioxidant components, rutin, quercetin, isoquercetin, astragalin, kaempferol, and kaempferol-3-o-rutoside, were identified in the EAE.

scholarly journals Extraction Process Optimization, Identification, and Profiling of Phenolic Antioxidant Compounds from the Fruits of Ficus auriculata

2020 ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Process ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Experimental Findings ◽  
Optimum Extraction

Abstract In this study, the extraction conditions to maximize the antioxidant activity and total phenolic content of Ficus auriculata were optimized using response surface methodology. For the purpose of extraction, the Ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P<0.0001) and total phenolic content (R2 = 0.961, P<0.0001), indicating a significant correlation between the experimental and expected value. The highest antioxidant activity (85.20 ± 0.96 %) for DPPH were achieved at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC-ESI-MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

Study on Extraction of Polyphenols from Black Soybeans and Antioxidant Activity

2014 ◽  
Vol 675-677 ◽  
pp. 1629-1633 ◽  
Author(s):  
Chun Ling Xiao ◽  
Ying Ren ◽  
Hui Ling Ding
Keyword(s):  
Antioxidant Activity ◽  
Power System ◽  
Ethanol Concentration ◽  
Raw Materials ◽  
Reducing Power ◽  
Extraction Yield ◽  
Black Beans ◽  
Polyphenol Extraction ◽  
Optimum Extraction

In this experiment black soybeans as raw materials,different factor on black beans polyphenol extraction yield influence,and studied the polyphenols antioxidant activity through establishing DPPH· system,·OH system and reducing power system,and compared with Vc. The result showed that:the optimum extraction conditions were the ratio of liquid to Solid was 32:1,ethanol concentration 48%,time 95min,temperature 90 °C.In these conditions, the yield of polyphenols was 8.953mg/g. The scavenging effects of polyphenols and Vc on DPPH·, ·OH force increased with increasing concentration,and the scavenging effects of polyphenols on DPPH· and ·OH higher than Vc when between in 11~31 μg/mL.When concentration is more than 12μg/mL,the scavenging effects of polyphenols on ·OH is higher than Vc.

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