Safflower yellow extract inhibits thrombus formation in mouse brain arteriole and exerts protective effects against hemorheology disorders in a rat model of blood stasis syndrome

Blood stasis syndrome (BSS) is one of the most common symptoms of cardiovascular diseases (CVDs) in traditional Chinese medicine (TCM) theory. Previous studies have identified that Salvia miltiorrhiza (Danshen) has beneficial effects on BSS, but there is no relevant research from the perspective of lipidomics to study the mechanism of Danshen against BSS since hyperlipidemia has been the widely accepted risk factor of CVDs. In this study, lipidomics technology combined with network pharmacology was applied to investigate the pathological mechanism of BSS and the protective effects of Danshen. The lipidomics profiling based on the UPLC-QTOF-MS analysis method was applied to identify the differential metabolites in the plasma of blood stasis rats. The related pathway and potential targets involved in the anti-BSS effects of Danshen were predicted by pathway analysis and network pharmacology. The biochemical results showed that Danshen intervention significantly reduced whole blood viscosity (WBV) at all the shear rates and fibrinogen concentration (FIB) p < 0.01 and increased activated partial thromboplastin time (APTT) effectively p < 0.01 . We also found that 52 lipid metabolites, including glycerophospholipid, sphingolipid, glycerolipid, plasmalogen, cholesterol ester, and testosterone, were associated with blood stasis. Moreover, Dgka, Hsd17b3, Hsd3b1, Inppl1, Lpl, Pik3ca, Pik3r1, Pla2g1b, Pla2g2a, Soat1, and Soat2 were predicted as potential targets, while glycerophospholipid metabolism, glycerolipid metabolism, steroid and steroid hormone biosynthesis, phosphatidylinositol signaling system, and ether lipid metabolism were involved as shared critical pathways of lipidomics analysis and network pharmacology. Collectively, this study offered a new understanding of the protection mechanism of Danshen against BSS, which provided new insight to explore the protective effects of Danshen.

Download Full-text

Protective effects of Naoxintong Capsule on rats with blood stasis syndrome

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2020.1820377 ◽

2020 ◽

Vol 34 (1) ◽

pp. 1077-1086

Author(s):

Wei-jian Zhang ◽

Wei-wei Su ◽

Qing-wei Lin ◽

Zeng-hao Yan ◽

Yong-gang Wang ◽

...

Keyword(s):

Blood Stasis ◽

Blood Stasis Syndrome ◽

Protective Effects

Download Full-text

Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model

RSC Advances ◽

10.1039/c7ra11748e ◽

2017 ◽

Vol 7 (89) ◽

pp. 56471-56483

Author(s):

Jing-Jing Xu ◽

Feng Xu ◽

Shu-Jie Shen ◽

Teng Li ◽

Yi-Fan Zhang ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Rat Model ◽

Blood Stasis ◽

Blood Stasis Syndrome ◽

Chinese Medicine Syndrome ◽

Dynamic Changes ◽

Metabolic Alterations ◽

Traditional Chinese Medicine Syndrome ◽

First Time

Dynamic changes of the metabolic network during the evolution of a syndrome based on the toxic heat and blood stasis syndrome (THBSS) rat model have been elucidated for the first time.

Download Full-text

Protective effects of Naoxintong capsule alone and in combination with ticagrelor and atorvastatin in rats with Qi deficiency and blood stasis syndrome

Pharmaceutical Biology ◽

10.1080/13880209.2020.1821066 ◽

2020 ◽

Vol 58 (1) ◽

pp. 1006-1022

Author(s):

Wei-jian Zhang ◽

Wei-wei Su ◽

Qing-wei Lin ◽

Yan He ◽

Zeng-hao Yan ◽

...

Keyword(s):

Blood Stasis ◽

Blood Stasis Syndrome ◽

Protective Effects

Download Full-text

Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia

Journal of Chinese Integrative Medicine ◽

10.3736/jcim20080114 ◽

2008 ◽

Vol 6 (1) ◽

pp. 73-76 ◽

Cited By ~ 1

Author(s):

Hai-feng Wei

Keyword(s):

Brain Ischemia ◽

Rat Model ◽

Blood Stasis ◽

Blood Stasis Syndrome ◽

Tongue Manifestation

Download Full-text

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/1514845 ◽

2019 ◽

Vol 2019 ◽

pp. 1-20 ◽

Cited By ~ 3

Author(s):

Nan Ran ◽

Zhiqiang Pang ◽

Xuewa Guan ◽

Guoqiang Wang ◽

Jinping Liu ◽

...

Keyword(s):

Rat Model ◽

Metabolic Pathways ◽

Acid Metabolism ◽

Blood Stasis ◽

Blood Stasis Syndrome ◽

Inflammatory Factors ◽

Control Group ◽

High Dose ◽

Alpha Linolenic Acid ◽

Model Control

In traditional Chinese medicine theory, blood stasis syndrome (BSS), characterized by blood flow retardation and blood stagnation, is one of the main pathologic mechanisms and clinical syndromes of cardiovascular diseases (CVDs). Rhodiola wallichiana var. cholaensis injection (RWCI) is made from dry roots and stems of RWC via the processes of decoction, alcohol precipitation, filtration, and dilution. Studies indicated the extracts of RWC could alleviate CVDs; however, the mechanism had not been illustrated. In the present study, the acute blood stasis rat model was established to investigate the pathogenesis of BSS and the therapeutic mechanism of RWCI against BSS. Hemorheological parameters (whole blood viscosity and plasma viscosity) and inflammatory factors (TNF-α and IL-6) were used to evaluate the success of the BSS rat model and RWCI efficacy. 14 and 33 differential metabolites were identified from plasma and urine samples using the metabolomics approach based on ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The results of multivariate analysis displayed that there were significant separations among model, control, and treatment groups, but the high-dose RWCI treatment group was closer to the control group. 9 perturbed metabolic pathways were related to BSS’s development and RWCI intervention. 5 metabolic pathways (arachidonic acid metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, retinol metabolism, and steroid hormone biosynthesis) showed apparent correlations. These differential metabolites and perturbed metabolic pathways might provide a novel view to understand the pathogenesis of BSS and the pharmacological mechanism of RWCI.

Download Full-text

Protective Effects of Total Flavones ofElaeagnus rhamnoides(L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/8142562 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Zhicheng Wei ◽

Fang Zuo ◽

Wenqian Wang ◽

Li Wang ◽

Dong Tong ◽

...

Keyword(s):

Rat Model ◽

Caspase 3 ◽

Blood Stasis ◽

Endothelial Injury ◽

Water Bath ◽

High Dose ◽

Vascular Endothelial ◽

Protective Effects ◽

Von Willebrand ◽

Ice Water

The aim was to evaluate the protective effects of total flavones ofElaeagnus rhamnoides(L.) A. Nelson (TFE) against vascular endothelial injury in blood stasis model rats and explore the potential mechanisms preliminarily. The model of blood stasis rat model with vascular endothelial injury was induced by subcutaneous injection of adrenaline combined with ice-water bath. Whole blood viscosity (WBV), histological examination, and prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) were measured. Meanwhile, the levels of Thromboxane B2 (TXB2), 6-keto-PGF1α, von Willebrand factor (vWF), and thrombomodulin (TM) were detected. In addition, Quantitative Real-Time PCR (qPCR) was performed to identify PI3K, Erk2, Bcl-2, and caspase-3 gene expression. The results showed that TFE can relieve WBV, increase PT and APTT, and decrease FIB content obviously. Moreover, TFE might significantly downregulate the levels of TXB2, vWF, and TM in plasma and upregulate the level of 6-keto-PGF1αin plasma. Expressions of PI3K and Bcl-2 were increased and the expression of caspase-3 was decreased by TFE pretreatment in the rat model. Consequently, the study suggested that TFE may have the potential against vascular endothelial injury in blood stasis model rats induced by a high dose of adrenaline with ice-water bath.

Download Full-text