scholarly journals Combined Lipidomics and Network Pharmacology Study of Protective Effects of Salvia miltiorrhiza against Blood Stasis Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yidian Jin ◽  
Zhiru Xie ◽  
Shasha Li ◽  
Xiangyu Zeng ◽  
Leqi Wang ◽  
...  
Keyword(s):  
Salvia Miltiorrhiza ◽  
Blood Stasis ◽  
Ether Lipid ◽  
Blood Stasis Syndrome ◽  
Network Pharmacology ◽  
Fibrinogen Concentration ◽  
Protective Effects ◽  
Critical Pathways ◽  
Protection Mechanism ◽  
Beneficial Effects

Blood stasis syndrome (BSS) is one of the most common symptoms of cardiovascular diseases (CVDs) in traditional Chinese medicine (TCM) theory. Previous studies have identified that Salvia miltiorrhiza (Danshen) has beneficial effects on BSS, but there is no relevant research from the perspective of lipidomics to study the mechanism of Danshen against BSS since hyperlipidemia has been the widely accepted risk factor of CVDs. In this study, lipidomics technology combined with network pharmacology was applied to investigate the pathological mechanism of BSS and the protective effects of Danshen. The lipidomics profiling based on the UPLC-QTOF-MS analysis method was applied to identify the differential metabolites in the plasma of blood stasis rats. The related pathway and potential targets involved in the anti-BSS effects of Danshen were predicted by pathway analysis and network pharmacology. The biochemical results showed that Danshen intervention significantly reduced whole blood viscosity (WBV) at all the shear rates and fibrinogen concentration (FIB) p < 0.01 and increased activated partial thromboplastin time (APTT) effectively p < 0.01 . We also found that 52 lipid metabolites, including glycerophospholipid, sphingolipid, glycerolipid, plasmalogen, cholesterol ester, and testosterone, were associated with blood stasis. Moreover, Dgka, Hsd17b3, Hsd3b1, Inppl1, Lpl, Pik3ca, Pik3r1, Pla2g1b, Pla2g2a, Soat1, and Soat2 were predicted as potential targets, while glycerophospholipid metabolism, glycerolipid metabolism, steroid and steroid hormone biosynthesis, phosphatidylinositol signaling system, and ether lipid metabolism were involved as shared critical pathways of lipidomics analysis and network pharmacology. Collectively, this study offered a new understanding of the protection mechanism of Danshen against BSS, which provided new insight to explore the protective effects of Danshen.

scholarly journals A Network Pharmacology Approach to Predict the Proangiogenesis Mechanism of Huangqi-Honghua Herb Pair after Cerebral Ischemia

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Jinyi Cao ◽  
Lu Lei ◽  
Kai Wang ◽  
Jing Sun ◽  
Yi Qiao ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Signaling Pathway ◽  
Target Genes ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Network Pharmacology ◽  
Western Blot Assay ◽  
Medicinal Value ◽  
Pharmacological Method

Objective. Huangqi-Honghua herb pair is known for its medicinal value to treat Qi deficiency and blood stasis syndrome with a long history in clinical practice. To understand its possible mechanism in a systematic study, a network pharmacological method was addressed. Methods. Detailed information on the HH compounds was obtained from two public databases, and oral bioavailability (OB) and drug-like (DL) of the compounds were evaluated. A correlation between HH compounds, its potential targets, and known targets was extrapolated, and the herb-compound-target-disease (H-C-T-D) network was established. Next, the pathway enrichment and essential genes were analyzed. Then, three key genes (VEGFA, VEGFR2, and eNOS), highly associated with angiogenesis, were screened and verified through western blot assay. Results. Out of 276 compounds, 21 HH compounds and 78 target genes regulating the major pathways associated with CI in the network are analyzed. The bioactive compounds in HH were active in various signal transduction pathways such as the toll-like receptor signaling pathway, VEGF signaling pathway, TNF signaling pathway, and HIF-1 signaling pathway are important pathways that may regulate anti-inflammatory, antiapoptotic, immune correlation, and antioxidative effects. The core genes are PTGS2, TNF, NOS2, IL6, BCL2, IL1B, SOD2, NOS3, SOD1, MMP9, and VEGFA. The in vitro results suggested that HH treatment could significantly elevate the expression of proangiogenic genes such as VEGFA, VEGFR2, and eNOS compared with OGD groups. Conclusions. Our results predict that HH may regulate the expression of VEGFA, VEGFR2, and eNOS via the VEGF and HIF-1 signaling pathway to promote angiogenesis and alleviate cerebral ischemia injury.

scholarly journals Protective effects of Naoxintong Capsule on rats with blood stasis syndrome

2020 ◽  
Vol 34 (1) ◽  
pp. 1077-1086
Author(s):  
Wei-jian Zhang ◽  
Wei-wei Su ◽  
Qing-wei Lin ◽  
Zeng-hao Yan ◽  
Yong-gang Wang ◽  
...  
Keyword(s):  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Protective Effects

scholarly journals Protective effects of Naoxintong capsule alone and in combination with ticagrelor and atorvastatin in rats with Qi deficiency and blood stasis syndrome

2020 ◽  
Vol 58 (1) ◽  
pp. 1006-1022
Author(s):  
Wei-jian Zhang ◽  
Wei-wei Su ◽  
Qing-wei Lin ◽  
Yan He ◽  
Zeng-hao Yan ◽  
...  
Keyword(s):  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Protective Effects

scholarly journals Deciphering the Active Compounds and Mechanisms of Qixuehe Capsule on Qi Stagnation and Blood Stasis Syndrome: A Network Pharmacology Study

2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Yu-Xi Huang ◽  
Ding-Qiao Xu ◽  
Shi-Jun Yue ◽  
Yan-Yan Chen ◽  
Hui-Juan Tao ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Network Pharmacology ◽  
Thrombin Time ◽  
Pharmacodynamic Effect ◽  
Menstrual Disorders ◽  
Active Compounds ◽  
Qi Stagnation ◽  
Underlying Mechanisms

Background. Qixuehe capsule (QXH), a Chinese patent medicine, has been demonstrated to be effective in the treatment of menstrual disorders. In traditional Chinese medicine (TCM) theory, qi stagnation and blood stasis syndrome (QS-BSS) is the main syndrome type of menstrual disorders. However, the pharmacodynamic effect of QXH in treating QS-BSS is not clear, and the main active compounds and underlying mechanisms remain unknown. Methods. A rat model of QS-BSS was established to evaluate the pharmacodynamic effect of QXH. Thereafter, a network pharmacology approach was performed to decipher the active compounds and underlying mechanisms of QXH. Results. QXH could significantly reduce the rising whole blood viscosity (WBV) and plasma viscosity (PV) but also normalize prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB) content in QS-BSS rats. Based on partial least-squares-discriminant analysis (PLS-DA), the low-dose QXH-intervened (QXH-L) and the high-dose QXH-intervened (QXH-H) groups seemed the most effective by calculating the relative distance to normality. Through network pharmacology, QXH may improve hemorheological abnormality mainly via 185 compounds-51 targets-28 pathways, whereas 184 compounds-68 targets-28 pathways were associated with QXH in improving coagulopathy. Subsequently, 25 active compounds of QXH were verified by UPLC-Q/TOF-MS. Furthermore, 174 active compounds of QXH were shared in improving hemorheological abnormality and coagulopathy in QS-BSS, each of which can act on multiple targets to be mainly involved in complement and coagulation cascades, leukocyte transendothelial migration, PPAR signaling pathway, VEGF signaling pathway, and arachidonic acid metabolism. The attribution of active compounds indicated that Angelicae Sinensis Radix (DG), Paeoniae Radix Rubra (CS), Carthami Flos (HH), Persicae Semen (TR), and Corydalis Rhizoma (YHS) were the vital herbs of QXH in treating QS-BSS. Conclusion. QXH can improve the hemorheology abnormality and coagulopathy of QS-BSS, which may result from the synergy of multiple compounds, targets, and pathways.

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