scholarly journals The influence of obesity on folate status, DNA methylation and cancer-related gene expression in normal breast tissues from premenopausal women

Epigenetics ◽  
2020 ◽  
pp. 1-10
Author(s):  
Armina-Lyn M. Frederick ◽  
Chi Guo ◽  
Ann Meyer ◽  
Liying Yan ◽  
Sallie S. Schneider ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Premenopausal Women ◽  
Normal Breast ◽  
Related Gene ◽  
Folate Status ◽  
Cancer Related Gene ◽  
Breast Tissues

Repression of protocadherin 17 is correlated with elevated angiogenesis and hypoxia markers in female patients with breast cancer

2021 ◽  
pp. 1-10
Author(s):  
Sanaa A. El-Benhawy ◽  
Samia A. Ebeid ◽  
Nadia A. Abd El Moneim ◽  
Rabie R. Abdel Wahed ◽  
Amal R.R. Arab
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Clinical Stage ◽  
Suppressor Gene ◽  
Normal Breast ◽  
Vital Role ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Cd34 Cells ◽  
Breast Tissues

BACKGROUND: Altered cadherin expression plays a vital role in tumorigenesis, angiogenesis and tumor progression. However, the function of protocadherin 17 (PCDH17) in breast cancer remains unclear. OBJECTIVE: Our target is to explore PCDH17 gene expression in breast carcinoma tissues and its relation to serum angiopoietin-2 (Ang-2), carbonic anhydrase IX (CAIX) and % of circulating CD34+ cells in breast cancer patients (BCPs). METHODS: This study included Fifty female BCPs and 50 healthy females as control group. Cancerous and neighboring normal breast tissues were collected from BCPs as well as blood samples at diagnosis PCDH17 gene expression was evaluated by RT-PCR. Serum Ang-2, CAIX levels were measured by ELISA and % CD34+ cells were assessed by flow cytometry. RESULTS: PCDH17 was downregulated in cancerous breast tissues and its repression was significantly correlated with advanced stage and larger tumor size. Low PCDH17 was significantly correlated with serum Ang-2, % CD34+ cells and serum CAIX levels. Serum CAIX, Ang-2 and % CD34+ cells levels were highly elevated in BCPs and significantly correlated with clinical stage. CONCLUSIONS: PCDH17 downregulation correlated significantly with increased angiogenic and hypoxia biomarkers. These results explore the role of PCDH17 as a tumor suppressor gene inhibiting tumor growth and proliferation.

Age-related gene expression and DNA methylation changes in rhesus macaque

Genomics ◽  
2020 ◽  
Vol 112 (6) ◽  
pp. 5147-5156
Author(s):  
Min Zhou ◽  
Liang Zhang ◽  
Qiao Yang ◽  
Chaochao Yan ◽  
Peng Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Rhesus Macaque ◽  
Related Gene ◽  
Age Related

Effects of in ovo feeding of vitamin C on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens

2020 ◽  
Vol 124 (9) ◽  
pp. 903-911 ◽  
Author(s):  
Yufei Zhu ◽  
Shizhao Li ◽  
Yulan Duan ◽  
Zhouzheng Ren ◽  
Xin Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Broiler Chickens ◽  
Immune Status ◽  
Stimulation Index ◽  
Average Daily Gain ◽  
Related Gene ◽  
In Ovo

AbstractThis study aimed to evaluate the effect of in ovo feeding (IOF) of vitamin C at embryonic age 11 (E11) on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens. A total of 240 Arbor Acres breeder eggs (63 (sem 0·5) g) were randomly divided into two groups: normal saline and vitamin C (VC) groups. After incubation, newly hatched chicks from each group were randomly divided into six replicates with ten chicks per replicate. Hatchability, average daily feed intake (D21–42 and D1–42), and average daily gain and feed conversion ratio (D1–21) were improved by vitamin C treatment (P < 0·05). IOF of vitamin C increased vitamin C content (D1), total antioxidant capacity (D42), IgA (D1), IgM (D1 and D21), stimulation index for T lymphocyte (D35) and lysozyme activity (D21) in plasma (P < 0·05). On D21, vitamin C increased the splenic expression of IL-4 and DNMT1 and decreased IL-1β, Tet2, Tet3 and Gadd45β expression (P < 0·05). On D42, vitamin C increased the splenic expression of IL-4 and DNMT3A and decreased IFN-γ, Tet3, MBD4 and TDG expression (P < 0·05). In conclusion, the vitamin C via in ovo injection can be absorbed by broiler’s embryo and IOF of vitamin C at E11 improves the post-hatch performance and immune status and, to some extent, the antioxidant capacity of broiler chickens. The expression of enzyme-related DNA methylation and demethylation indicates that the level of DNA methylation may increase in spleen in the VC group and whether the fluctuating expression of pro- and anti-inflammatory cytokines is related to DNA methylation change remained to be further investigated.

scholarly journals Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene PIK3R1 after Roux-en-Y Gastric Bypass

2020 ◽  
Vol 21 (12) ◽  
pp. 4476
Author(s):  
Marcela A S Pinhel ◽  
Natália Y Noronha ◽  
Carolina F Nicoletti ◽  
Vanessa AB Pereira ◽  
Bruno AP de Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Weight Loss ◽  
Gastric Bypass ◽  
Related Gene ◽  
Cpg Sites ◽  
Genome Wide ◽  
Genetically Determined ◽  
Methylation Patterns ◽  
Weight Loss Interventions

Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation of PIK3R1, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 ± 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, the PIK3R1 gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that the PI3KR1 gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways.

scholarly journals Detection of cancer-related gene expression profiles in severe cervical neoplasia

2004 ◽  
Vol 112 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Igor Sopov ◽  
Till Sörensen ◽  
Mandy Magbagbeolu ◽  
Lars Jansen ◽  
Katrin Beer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cervical Neoplasia ◽  
Related Gene ◽  
Cancer Related Gene

Gene expression patterns distinguish breast carcinomas from normal breast tissues: The Malaysian context

2010 ◽  
Vol 206 (4) ◽  
pp. 223-228 ◽  
Author(s):  
Ivyna Bong Pau Ni ◽  
Zubaidah Zakaria ◽  
Rohaizak Muhammad ◽  
Norlia Abdullah ◽  
Naqiyah Ibrahim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Normal Breast ◽  
Gene Expression Patterns ◽  
Breast Carcinomas ◽  
Breast Tissues

scholarly journals Integrative Analysis Reveals the Transcriptional Collaboration between EZH2 and E2F1 in the Regulation of Cancer-Related Gene Expression

2015 ◽  
Vol 14 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Han Xu ◽  
Kexin Xu ◽  
Housheng H. He ◽  
Chongzhi Zang ◽  
Chen-Hao Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Integrative Analysis ◽  
Related Gene ◽  
Cancer Related Gene
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