Potentiation of the Antibiotic Activity by the Essential Oils of Eugenia brasiliensis Lam. and Piper mosenii C. DC.

Antimicrobial resistance is recognized as one of the major global health challenges of the 21st century. Synergistic combinations for antimicrobial therapies can be a good strategy for the treatment of multidrug resistant infections. We examined the ability of a group of 29 plant essential oils as substances which enhance the antibiotic activity. We used a modified well diffusion method to establish a high-throughput screening method for easy and rapid identification of high-level enhancement combinations against bacteria. We found that 25 essential oils possessed antibacterial activity against Escherichia Coli ATCC 25922 and methicillin-resistant Staphylococcus aureus (MRSA) 43300 with MICs that ranged from 0.01% to 2.5% v/v. We examined 319 (11 × 29) combinations in a checkerboard assay with E. Coli ATCC 25922 and MRSA 43300, and the result showed that high-level enhancement combinations were 48 and 44, low-level enhancement combinations were 214 and 211, and no effects combinations were 57 and 64, respectively. For further verification we randomly chose six combinations that included orange and Petitgrain essential oils in a standard time-killing assay. The results are in great agreement with those of the well diffusion assays. Therefore, the modified diffusion method was a rapid and effective method to screen high-level enhancement combinations of antibiotics and essential oils.

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Essential oils from Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2011.05.005 ◽

2011 ◽

Vol 38 (4) ◽

pp. 325-330 ◽

Cited By ~ 44

Author(s):

Mariam Fadli ◽

Jacqueline Chevalier ◽

Asmaa Saad ◽

Nour-Eddine Mezrioui ◽

Lahcen Hassani ◽

...

Keyword(s):

Essential Oils ◽

Antibiotic Activity ◽

Gram Negative Bacteria ◽

Gram Negative

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Essential Oils and Their Components as Modulators of Antibiotic Activity against Gram-Negative Bacteria

Medicines ◽

10.3390/medicines3030019 ◽

2016 ◽

Vol 3 (3) ◽

pp. 19 ◽

Cited By ~ 17

Author(s):

Petruta Aelenei ◽

Anca Miron ◽

Adriana Trifan ◽

Alexandra Bujor ◽

Elvira Gille ◽

...

Keyword(s):

Essential Oils ◽

Antibiotic Activity ◽

Gram Negative Bacteria ◽

Gram Negative

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Electrochemical and Optical Microbiological Testing: a Comparative Study on Properties of Essential Oils

Food Processing Techniques and Technology ◽

10.21603/2074-9414-2020-4-650-659 ◽

2020 ◽

Vol 50 (4) ◽

pp. 650-659

Author(s):

Vladimir Sibirtsev ◽

Uliana Nechiporenko ◽

Vladimir Kabanov ◽

Mikhail Kukin

Keyword(s):

Biological Activity ◽

Essential Oils ◽

Food Products ◽

Antibiotic Activity ◽

Raw Material ◽

Exact Nature ◽

Cupressus Sempervirens ◽

Microbiological Methods ◽

Microbiological Testing ◽

Express Methods

Introduction. The national economy is currently developing a system for monitoring the quality and safety of goods. Food products, ingredients, and additives possess various pro- and antibiotic properties. Therefore, available express methods of quantitative assessment of microbiological contamination are a relevant aspect of domestic food industry. Study objects and methods. The study featured ten essential oils of plant origin that can be used as functional additives to various food products. Results and its discussion. The research introduced a new biotesting technique for repetitive recording of changes in the intensity of elastic light dispersion. The technique made it possible to measure pH and electrical conductivity of a liquid nutrient medium incubated in the presence and absence of viable test microorganisms and test samples. The paper describes the results of this technique applied to a comparative analysis of antibiotic activity of various essential oils in different concentrations. As the concentrations of the test extracts decreased, their antibiotic activity monotonically also went down, while the probiotic activity increased. The shortterm biological activity of test samples appeared to be significantly higher than their long-term activity. The medium-term biological activity of the test samples was mostly intermediate in value. Only rarely did it exceed both the long- and short-term biological activity of the same TE. The essential oils obtained from the leaves of Thuja occidentalis, Eucalyptus globulus, and Cupressus sempervirens exhibited the most active and long antibiotic properties. Conclusion. The biological activity of food products, including various plant extracts, depends not only on the raw material and the extraction method, but also on the concentration of the extract in the product. As a rule, the exact nature of these dependencies can only be established empirically and requires a set of various tests. The present article introduces a new highly objective and informative express methodology that simplifies this process. The technique is less labor-, time-, and material-consuming than standard visual microbiological methods. It can be used to assess the effect of test samples on the vital activity of microorganisms in various foods, ingredients, and additives.

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Terpenes, Antibacterial and Modulatory Antibiotic Activity of Essential Oils fromCroton hirtusL’ Hér. (Euphorbiaceae) from Ivory Coast

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2014.958550 ◽

2014 ◽

Vol 17 (4) ◽

pp. 607-616 ◽

Cited By ~ 4

Author(s):

Touré Daouda ◽

Kouamé Prevost ◽

Bedi Gustave ◽

Djaman A. Joseph ◽

Guesssennd Nathalie ◽

...

Keyword(s):

Essential Oils ◽

Ivory Coast ◽

Antibiotic Activity

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Electrochemical biotesting technique as applied to comparative assessment of antimicrobial properties of essential oils

Токсикологический вестник ◽

10.36946/0869-7922-2021-29-3-50-55 ◽

2021 ◽

pp. 50-55

Author(s):

V. S. Sibirtsev ◽

U. Yu. Nechiporenko ◽

V. L. Kabanov ◽

M. Yu. Kukin

Keyword(s):

Essential Oils ◽

Culture Medium ◽

Raw Materials ◽

Antimicrobial Properties ◽

Antibiotic Activity ◽

Visual Methods ◽

Vital Activity ◽

Plant Raw Materials ◽

Liquid Culture Medium ◽

Microbiological Testing

A biotesting technique is described that provides for periodic (every 2 hours) recording of changes in pH, redox potential, and electrical conductivity of a liquid culture medium incubated in the presence and in the absence of viable test microorganisms (TM) and test samples (TS). The results of a comparative analysis using this technique of antibiotic activity against Staphylococcus aureus of different concentrations of «essential oils» obtained from 10 types of plant raw materials are presented. Based on this, we can conclude the following. Using the presented methodology, it is possible to assess the effect on the dynamics of the vital activity of TM of samples of various pharmaceutical, cosmetic, food, feed and other products, much more quickly, objectively and informatively than using standard visual methods of microbiological testing. The initial antibiotic activity of TS in most cases was greater than their prolonged antibiotic activity. At the same time, the mid-term (in terms of the time of interaction of TS with TM) antibiotic activity of TS was usually intermediate in value between their initial and prolonged biological activity.

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Fibrous wound dressings encapsulating essential oils as natural antimicrobial agents

Journal of Materials Chemistry B ◽

10.1039/c4tb01974a ◽

2015 ◽

Vol 3 (8) ◽

pp. 1583-1589 ◽

Cited By ~ 76

Author(s):

I. Liakos ◽

L. Rizzello ◽

H. Hajiali ◽

V. Brunetti ◽

R. Carzino ◽

...

Keyword(s):

Essential Oils ◽

Cellulose Acetate ◽

Antimicrobial Agents ◽

Antibiotic Activity ◽

Wound Dressings ◽

Natural Polymer ◽

Natural Antimicrobial ◽

Electrospinning Technique

Essential oils with high antibiotic activity were incorporated into cellulose acetate natural polymer. By using the electrospinning technique, nanofibrous matrices were prepared to be used as effective antimicrobial wound dressings.

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Gas chromatography coupled to mass spectrometry (GC-MS) characterization and evaluation of antibacterial bioactivities of the essential oils from Piper arboreum Aubl., Piper aduncum L. e Piper gaudichaudianum Kunth

Zeitschrift für Naturforschung C ◽

10.1515/znc-2020-0045 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Ana Cristina A. da Silva ◽

Edinardo F. F. Matias ◽

Janaína E. Rocha ◽

Ana Carolina Justino de Araújo ◽

Thiago S. de Freitas ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oils ◽

Inhibitory Effect ◽

Antibiotic Activity ◽

Chemical Components ◽

Synergistic Activity ◽

Significant Activity ◽

Piper Aduncum ◽

Piper Gaudichaudianum

AbstractThe objective of this study was to determine the chemical profile and to evaluate the antibacterial activity of the essential oils of Piper species and modulation of the antibiotic activity, using the microdilution method to determine the minimum inhibitory concentration. The chemical components were characterized by gas chromatography coupled to mass spectrometry, which revealed β-copaen-4-α-ol (31.38%), spathulenol (25.92%), and germacrene B (21.53%) as major constituents of the essential oils of Piper arboreum, Piper aduncum, and Piper gaudichaudianum, respectively. The essential oils analyzed in this study did not present a clinically relevant activity against standard and multiresistant Escherichia coli. However, in the case of multiresistant Staphylococcus aureus, there was a significant activity, corroborating with reports in the literature, where Gram-positive bacteria are more susceptible to antimicrobial activity. The essential oils modulated the effect of the antibiotics norfloxacin and gentamicin, having on the latter greater modulating effect; however, for erythromycin, no statistically significant effect was observed. In conclusion, the results obtained in this study demonstrated that the essential oils of the analyzed Piper species present an inhibitory effect against S. aureus and modulate antibiotic activity, most of which presents synergistic activity.

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