Experimental design applications in chemistry: A case study on Central composite design for optimization of extraction of olive seeds in a Soxhlet

2019 ◽  
Vol 5 (3) ◽  
pp. 189-199
Author(s):  
Oğuz Akpolat
Keyword(s):  
Experimental Design ◽  
Central Composite Design ◽  
Olive Seeds ◽  
Central Composite

Optimization of Linoleic Acid Monoepoxidation Condition from Malaysian Jatropha curcas Using Central Composite Design (CCD)

2015 ◽  
Vol 754-755 ◽  
pp. 1107-1112
Author(s):  
Rozaini Abdullah ◽  
Jumat Salimon ◽  
Anis Atikah Ahmad
Keyword(s):  
Linoleic Acid ◽  
Reaction Time ◽  
Experimental Design ◽  
Central Composite Design ◽  
Reaction Temperature ◽  
Jatropha Curcas ◽  
Catalyst Loading ◽  
Time Saving ◽  
Independent Variable ◽  
Central Composite

The aim of this study was to optimize the monoepoxidation process of linoleic acid obtained from Malaysian Jatropha curcas oil using central composite design (CCD). There were four independent variable factors had been studied which involved reaction temperature (X1), reaction time (X2), catalyst loading (X3) and H2O2 concentration (X4). Thirty experiments were carried out based on the experimental design responses obtained. The results showed that the optimum condition was obtained at catalyst loading of 0.11% (w/w) methyltrioxorhernium (VII) (MTO), H2O2 mole of 99%, reaction temperature of 58.41oC for 5 hours. The central composite design was proven to be simpler method, time saving and required less samples compared to the conventional method.

Optimization of Critical Medium Components for the Expression of Recombinant Human Transferrin in Insect Cells Baculovirus System

2012 ◽  
Author(s):  
Clarence M. Ongkudon ◽  
Badarulhisam Abdul Rahman ◽  
Azila Abd. Aziz
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Central Composite Design ◽  
Response Surface ◽  
Insect Cells ◽  
Expression System ◽  
Proliferating Cells ◽  
Human Transferrin ◽  
Lipid Mixtures ◽  
Central Composite

Transferin manusia (hTf) memainkan peranan yang penting dalam fungsi bakteriostatik dan pengangkutan ferum dari bahagian penyimpanan ke sel–sel yang membiak melalui proses endositosis janaan reseptor. Sistem ekspresi bakulovirus sel serangga telah dipakai secara meluas sebagai sistem alternatif dalam penghasilan Transferin manusia rekombinan (rhTf). Kajian ini ditumpukan ke atas pengoptimuman glutamina, glukosa dan campuran lipid 1000x yang dapat meningkatkan penghasilan rhTf. Reka bentuk eksperimen yang melibatkan 17 eksperimen reka bentuk komposit berpusat (CCD) telah digunakan dan hasil kajian dianalisis oleh Statistika (Statsoft v. 5.0). Metodologi permukaan tindak balas (RSM) telah mengenalpasti nilai optimum parameterparameter yang dikaji iaitu glutamina=2211.20 mg/L, glukosa=1291.95 mg/L, dan campuran lipid 1000x=0.64 %v/v. Hasil optimasi menunjukkan peningkatan hasil rhTf sebanyak tiga kali ganda, iaitu daripada 19.89 μg/ml kepada 65.12 μg/ml. Kata kunci: Transferin manusia; bakulovirus sel serangga; reka bentuk eksperimen; reka bentuk komposit berpusat; metodologi permukaan tindak balas Human Transferrin (hTf) plays a big role in providing bacteriostatic functions as well as to transport iron from the storage part to all proliferating cells by receptor mediated endocytosis. Insect cells baculovirus expression system has been widely used as an alternative expression system for the production of recombinant human Transferrin (rhTf). This work focused mainly on the optimization of glutamine, glucose and lipid mixtures 1000x to increase rhTf yield. An experimental design involving 17 central composite design (CCD) experiments was employed and results were analyzed by Statistica (Statsoft v. 5.0). The response surface methodology (RSM) had identified the optimum values where glutamine=2211.20 mg/L, glucose=1291.95 mg/L, and lipid mixtures 1000x=0.64 %v/v. Using the optimized parameters, the studies demonstrated an increase in the rhTf yield by three–fold from 19.89 μg/ml to 65.12 μg/ml. Key words: Human transferrin; insect cells baculovirus; experimental design; central composite design; response surface methodology

scholarly journals Optimization of chlorite and talc flotation using experimental design methodology: Case study of the MCG plant, Morocco

2020 ◽  
Vol 120 (12) ◽  
Author(s):  
K. Boujounoui ◽  
A. Abidi ◽  
A. Baçaoui ◽  
K. El Amari ◽  
A. Yaacoubi
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Desirability Functions ◽  
Sulphide Ore ◽  
Experimental Values ◽  
Experimental Design Methodology ◽  
Central Composite ◽  
Good Agreement

SYNOPSIS Response surface methodology (RSM), central composite design (CCD), and desirability functions were used for modelling and optimization of the operating factors in chlorite and talc (collectively termed 'mica') flotation. The influence of pulp pH, cyanide (NaCN) consumption, and particle size was studied with the aim of optimizing ssilicate flotation while minimizing recoveries of galena, chalcopyrite, and sphalerite. Flotation tests were carried out on a representative sample of a complex sulphide ore from Draa Sfar mine (Morocco). The model predictions for the flotation of each of the minerals concerned were found to be in good agreement with experimental values, with R2 values of 0.91, 0.98, 0.99, and 0.90 for mica, galena, chalcopyrite, and sphalerite recoveries, respectively. RSM combined with desirability functions and CCD was successfully applied for the modelling of mica flotation, considering simultaneously the four flotation responses to achieve the maximum recovery of mica and minimal loss of Pb, Cu, and Zn to the flotation concentrate. Keywords: chlorite, talc, flotation, response surface methodology, central composite design, optimization.

scholarly journals Central Composite Design for Response Surface Methodology and Its Application in Pharmacy

2021 ◽  
Author(s):  
Sankha Bhattacharya
Keyword(s):  
Central Composite Design ◽  
Response Surface ◽  
Response Surface Model ◽  
Fractional Factorial ◽  
Surface Model ◽  
Experimental Conditions ◽  
Design Models ◽  
Central Composite

The central composite design is the most commonly used fractional factorial design used in the response surface model. In this design, the center points are augmented with a group of axial points called star points. With this design, quickly first-order and second-order terms can be estimated. In this book chapter, different types of central composite design and their significance in various experimental design were clearly explained. Nevertheless, a calculation based on alpha (α) determination and axial points were clearly described. This book chapter also amalgamates recently incepted central composite design models in various experimental conditions. Finally, one case study was also discussed to understand the actual inside of the central composite design.

Gelatin/poly(vinyl alcohol) based hydrogel film – A potential biomaterial for wound dressing: Experimental design and optimization followed by rotatable central composite design

2021 ◽  
pp. 088532822199226
Author(s):  
Patricia Hubner ◽  
Nilson Romeu Marcilio ◽  
Isabel Cristina Tessaro
Keyword(s):  
Experimental Design ◽  
Central Composite Design ◽  
Vinyl Alcohol ◽  
Biomedical Applications ◽  
Poly Vinyl Alcohol ◽  
Swelling Kinetics ◽  
Mechanical Resistance ◽  
Hydrogel Film ◽  
Central Composite ◽  
Hydrogel Films

The development of hydrogel films for biomedical applications is interesting due to their characteristics. Hydrogel films based on gelatin and poly(vinyl alcohol) (PVA) are developed and characterized using a rotatable central composite design. The optimized hydrogel film is obtained by the function desirability of the Statistica® software and is also characterized by swelling kinetics, oxygen permeability, adhesiveness, TGA, DSC, and XRD. The results of the experimental design show that gelatin and PVA concentrations have a significant influence on the response variables, and the exposure doses to UV light show no significant effect. The optimized hydrogel film is elastic, presents good mechanical resistance and swelling capacity in water and exudate solution, is permeable to oxygen, and is capable of adjusting itself and maintains contact close to the skin. In this way, considering all the properties evaluated, the optimized film has characteristics suitable for biomedical applications as wound dressings.

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