scholarly journals Secondary induction of cytotoxic T lymphocytes with solubilized syngeneic tumor cell plasma membranes.

1978 ◽  
Vol 148 (5) ◽  
pp. 1435-1439 ◽  
Author(s):  
O Alaba ◽  
L W Law
Keyword(s):  
Plasma Membrane ◽  
T Lymphocytes ◽  
Tumor Cell ◽  
Cytotoxic T Lymphocytes ◽  
Plasma Membranes ◽  
Effector Cells ◽  
Syngeneic Tumor ◽  
Cell Plasma

Secondary induction of in vitro cytotoxic T lymphocytes in a syngeneic system has been achieved with plasma membrane, both in the particulate and solubilized forms. Both the induction and the lytic phases were shown to be immunologically specific. The effector cells generated were completely susceptible to treatment with anti-theta antibody and complement, suggesting that they are T lymphocytes.

Suppression of tumor cell growth by allospecific cytotoxic T-lymphocytes in vitro

2007 ◽  
Vol 413 (1) ◽  
pp. 172-175
Author(s):  
T. V. Anfalova ◽  
L. M. Khromykh ◽  
D. B. Kazanskii
Keyword(s):  
Cell Growth ◽  
T Lymphocytes ◽  
Tumor Cell ◽  
Cytotoxic T Lymphocytes ◽  
Tumor Cell Growth

scholarly journals Low dose radiosensitivity of alloimmune cytotoxic T cells.

1982 ◽  
Vol 155 (6) ◽  
pp. 1858-1863 ◽  
Author(s):  
C Spellman ◽  
R E Anderson
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Cell Cultures ◽  
Low Dose ◽  
Cytotoxic T Cells ◽  
X Rays ◽  
Effector Cells ◽  
Chromium Release

Low dose radiosensitivity of in vitro generated alloimmune murine cytotoxic T lymphocytes (CTL) was studied. It appears that a subset of CTL exists that can be killed with 10-25 rad of x rays. These radiosensitive CTL are Lyt-1,2+ T lymphocytes. Analyses of cytotoxicity by chromium release assays indicate that the radiosensitive CTL are present in responder spleen cell cultures from all strains of mice tested. The generation of these effector cells is most pronounced in animals of the C57BL background. The mechanism of low dose radiosensitivity appears to be interphase death.

scholarly journals Loss of the transferrin receptor during the maturation of sheep reticulocytes in vitro. An immunological approach

1983 ◽  
Vol 210 (1) ◽  
pp. 37-47 ◽  
Author(s):  
B T Pan ◽  
R Blostein ◽  
R M Johnstone
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Transferrin Receptor ◽  
Plasma Membranes ◽  
Plasma Membrane Proteins ◽  
Molecular Weights ◽  
Subunit Molecular Weight ◽  
Cell Plasma ◽  
A Subunit

Sheep reticulocyte-specific antiserum absorbed with mature sheep red cells has been used to isolate and identify reticulocyte-specific plasma-membrane proteins and to monitor their loss during incubation in vitro. Specific precipitation of labelled plasma-membrane proteins is obtained when detergent-solubilized extracts of 125I-labelled reticulocyte plasma membranes are incubated with this antiserum and Staphyloccus aureus, but not when mature-cell plasma membranes are treated similarly. During maturation of reticulocytes in vitro (up to 4 days at 37 degrees C), there is a marked decrease in the immunoprecipitable material. The anti-reticulocyte-specific antibodies have been identified as anti-(transferrin receptor) antibodies. By using these antibodies as a probe, the transferrin receptor has been shown to have a subunit molecular weight of 93 000. The data are consistent with reported molecular weights of this receptor and with the proposal that the receptor may exist as a dimer, since [125I]iodotyrosyl-peptide maps of the 93 000- and 186 000-mol.wt. components isolated are shown to be identical. Evidence is presented for the transmembrane nature of the receptor and for the presence of different binding sites for transferrin and these antibodies on the receptor.

Effector cells of low-dose IL-2 immunotherapy in tumor bearing mice: Tumor cell killing by CD8+ cytotoxic T lymphocytes and macrophages

Immunobiology ◽  
1992 ◽  
Vol 186 (3-4) ◽  
pp. 214-229 ◽  
Author(s):  
Riks A. Maas ◽  
Pauline A.M. Roest ◽  
Martin J. Becker ◽  
Iris S. Weimar ◽  
Hub F.J. Dullens ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cell ◽  
Cytotoxic T Lymphocytes ◽  
Low Dose ◽  
Cell Killing ◽  
Effector Cells ◽  
Tumor Bearing ◽  
Tumor Cell Killing

scholarly journals CYTOSTATIC ELIMINATION OF SYNGENEIC RAT TUMOR CELLS IN VITRO BY NONSPECIFICALLY ACTIVATED MACROPHAGES

1973 ◽  
Vol 138 (3) ◽  
pp. 625-644 ◽  
Author(s):  
R. Keller
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Polymorphonuclear Leukocytes ◽  
Morphological Changes ◽  
Substantial Reduction ◽  
Cell Contact ◽  
Target Cells ◽  
Effector Cells ◽  
Syngeneic Tumor

Syngeneic tumor cell lines induced in inbred DA rats by polyoma virus, dimethylbenzanthracene, or methylcholanthrene were interacted in vitro with syngeneic effector cells. Glycogen-induced peritoneal exudate cells, predominantly polymorphonuclear leukocytes, and proteose peptone-induced peritoneal cells, principally macrophages, were the effector cells employed. Activated, nonimmune macrophages or exudative polymorphonuclear leukocytes produced pronounced morphological changes in syngeneic tumor cells as evidenced by a substantial reduction in tumor cell numbers and appearance of shrunken cells, even though there was no increase in cell debris. Polymorphonuclear leukocytes exerted a generally similar but quantitatively much diminished effect. These effector cells constantly produced a decrease in the incorporation by tumor cells of DNA precursors such as [3H]thymidine and of RNA precursors such as [3H]uridine. In this regard, the effector cells were quite refractory to high doses of X-irradiation. Interaction of target cells with activated, nonimmune macrophages yielded low but consistent signs of cytotoxicity, whereas polymorphonuclear leukocytes gave no such effects. Elimination of functional macrophages by silica, an agent specifically toxic for macrophages, resulted in unrestricted tumor cell proliferation despite continued generation of cytotoxicity. Accordingly, cytostatic mechanisms appear to play a predominant role in the elimination of tumor cells by nonimmune phagocytes. Evidence from a variety of experimental approaches suggest that the cytostatic effect is dependent on cell-to-cell contact.

scholarly journals Simultaneous Induction of Multiple Antigen-Specific Cytotoxic T Lymphocytes in Nonhuman Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids

1998 ◽  
Vol 66 (9) ◽  
pp. 4193-4202 ◽  
Author(s):  
Ruobing Wang ◽  
Denise L. Doolan ◽  
Yupin Charoenvit ◽  
Richard C. Hedstrom ◽  
Malcolm J. Gardner ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Peripheral Blood Mononuclear ◽  
Effector Cells ◽  
Cell Responses

ABSTRACT CD8+ T cells have been implicated as critical effector cells in protective immunity against malaria parasites developing within hepatocytes. A vaccine that protects against malaria by inducing CD8+ T cells will probably have to include multiple epitopes on the same protein or different proteins, because of parasite polymorphism and genetic restriction of T-cell responses. To determine if CD8+ T-cell responses against multiple P. falciparum proteins can be induced in primates by immunization with plasmid DNA, rhesus monkeys were immunized intramuscularly with a mixture of DNA plasmids encoding four P. falciparumproteins or with individual plasmids. All six monkeys immunized with PfCSP DNA, seven of nine immunized with PfSSP2 DNA, and five of six immunized with PfExp-1 or PfLSA-1 DNA had detectable antigen-specific cytotoxic T lymphocytes (CTL) after in vitro restimulation of peripheral blood mononuclear cells. CTL activity was genetically restricted and dependent on CD8+ T cells. By providing the first evidence for primates that immunization with a mixture of DNA plasmids induces CD8+ T-cell responses against all the components of the mixture, these studies provide the foundation for multigene immunization of humans.

scholarly journals Self-sparing of long-term in vitro-cloned or uncloned cytotoxic T lymphocytes.

1986 ◽  
Vol 164 (3) ◽  
pp. 962-967 ◽  
Author(s):  
M F Luciani ◽  
J F Brunet ◽  
M Suzan ◽  
F Denizot ◽  
P Golstein
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Cytotoxic T Cell ◽  
Cell Populations ◽  
Con A ◽  
T Cell Clones ◽  
Cell Clones

At least some long-term in vitro-cultured cytotoxic T cell clones and uncloned cell populations are able, in the presence of Con A, to lyse other cells, to be lysed by other cells, but not to lyse themselves. This as-yet-unexplained result may have implications as to the mechanism of T cell-mediated cytotoxicity.

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