scholarly journals Bacillus and azotobacter counts in solid biofertilizer with different concentration of zeolite and liquid inoculant

2021 ◽  
Vol 667 (1) ◽  
pp. 012010
Author(s):  
R Hindersah ◽  
I Rahmadina ◽  
R Harryanto ◽  
P Suryatmana ◽  
M Arifin
Keyword(s):  
Liquid Inoculant

Effects of spray inoculation equipment on the viability of Rhizobium spp. in liquid inoculants for legumes

1982 ◽  
Vol 22 (117) ◽  
pp. 299 ◽  
Author(s):  
RR Gault ◽  
DL Chase ◽  
J Brockwell
Keyword(s):  
Soybean Seed ◽  
Strain Differences ◽  
Significant Loss ◽  
Operating Pressure ◽  
Test Bed ◽  
Spray Inoculation ◽  
Rhizobium Spp ◽  
Set Up ◽  
Pumping Equipment ◽  
Liquid Inoculant

Pumping equipment designed for seedbed inoculation of legumes with liquid inoculants was set up on a test bed in the laboratory. Experiments, in which liquid inoculant was circulated through the equipment, were conducted to determine the effect on inoculant viability of variables likely to be encountered when farmers used the equipment in the field. Provided that peat cultures of Rhizobium spp. were used to make liquid inoculant, neither pump type, operating pressure up to 173 kPa, water temperature up to 35�C, nor water impurity up to a level equivalent to 170 �S/cm conductivity seriously reduced inoculant populations during the first hour of treatment, although a significant decline in numbers occurred in three out of 16 experiments. When inoculant was exposed to the various treatments for long periods, a significant loss of viability occurred, in 7 out of 13 experiments, between 4-8 h. R. meliloti was least affected by treatment and R. leguminosarum most affected, but this may have been due to strain differences as much as to species differences. Liquid inoculants which were made from broth cultures lost viability very quickly. R.. meliloti liquid inoculant, prepared from a peat culture and introduced by spraying into a dry soil of neutral pH in the absence of any host plant, did not lose viability during a period of four weeks. The spray inoculation equipment was also used successfully in a field experiment to add water to the seedbed to aid in the germination of soybean seed sown into drying soil.

scholarly journals Chemical characteristics of organic-based liquid inoculant of Bacillus spp

2019 ◽  
Vol 393 ◽  
pp. 012005
Author(s):  
R Hindersah ◽  
M R Setiawati ◽  
B N Fitriatin ◽  
P Suryatmana ◽  
P Asmiran
Keyword(s):  
Chemical Characteristics ◽  
Bacillus Spp ◽  
Liquid Inoculant

Rhizobial inoculant formulations and soil pH influence field pea nodulation and nitrogen fixation

2000 ◽  
Vol 80 (3) ◽  
pp. 395-400 ◽  
Author(s):  
W. A. Rice ◽  
G. W. Clayton ◽  
P. E. Olsen ◽  
N. Z. Lupwayi
Keyword(s):  
Nitrogen Content ◽  
Soil Ph ◽  
Crop Production ◽  
Plant Biomass ◽  
Acid Soil ◽  
Dry Weight ◽  
Field Pea ◽  
Total Biomass ◽  
Plant Nitrogen ◽  
Liquid Inoculant

Crop production systems that include field pea (Pisum sativum L.) in rotation are important for sustainable agriculture on acid soils in northwestern Canada. Greenhouse experiments were conducted to compare the ability of liquid inoculant applied to the seed, powdered peat inoculant applied to the seed, and granular inoculant applied in a band with the seed to establish effective nodulation on field pea grown at soil pH(H2O) 4.4, 5.4 and 6.6. Plants were grown to the flat pod stage, and then total plant biomass dry weight, dry weight of nodules, number of nodules, plant nitrogen content, and proportion of plant nitrogen derived from the atmosphere (%Ndfa) were measured. Granular and powdered peat inoculants produced greater nodule numbers and weight, plant nitrogen content, %Ndfa and total biomass than liquid inoculant in at least two of the three experiments. Only granular inoculant was effective in establishing nodules at soil pH 4.4, but granular and powdered peat inoculants were effective at pH 5.4, and all three formulations were effective at pH 6.6. The results showed that granular inoculant has potential for effective nodulation of field pea grown on acid soil. Key words: Rhizobium, inoculant formulations, field pea, nodulation, acid soil

scholarly journals In-furrow inoculation of soybean as alternative to fungicide and micronutrient seed treatment

2010 ◽  
Vol 34 (4) ◽  
pp. 1103-1112 ◽  
Author(s):  
Rubens José Campo ◽  
Ricardo Silva Araujo ◽  
Fábio Luís Mostasso ◽  
Mariangela Hungria
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Attractive Alternative ◽  
Negative Effects ◽  
Major Barrier ◽  
Biological N2 Fixation ◽  
Growing Seasons ◽  
Grain Crop ◽  
First Time ◽  
Liquid Inoculant

Soybean is a major grain crop in Brazil, and yields can be considerably improved by inoculation with selected Bradyrhizobium strains. However, the incompatibility between inoculation and seed treatments with fungicides and micronutrients represents a major barrier to the achievement of high rates of biological N2 fixation. Inoculation practices that can alleviate the negative effects of agrochemicals must therefore be found and in-furrow inoculation seems to be an attractive alternative. This study reports the results of seven field experiments conducted in three growing seasons in Brazil; three in soils previously cropped with inoculated soybean (> 10(4) cells g-1 of soil of Bradyrhizobium), and four in areas where the crop was sown for the first time (< 10² cells g-1 of soil of Bradyrhizobium). The compatibility with fungicides and micronutrients was compared in seeds inoculated with peat or liquid inoculants, or treated with different doses of liquid inoculant in-furrow. In areas with established Bradyrhizobium populations, seed-applied agrochemicals did generally not affect nodulation, but also did not increase yields, while inoculation always increased N grain accumulation or yield, and N fertilizer decreased both nodulation and yield. Where soybean was sown for the first time, the seed treatment with agrochemicals affected nodulation when applied together with peat or liquid inoculant. In-furrow inoculation alleviated the effects of seed treatment with agrochemicals; the best performance was achieved with high Bradyrhizobium cell concentrations, with up to 2.5 million cells seed-1.

scholarly journals EFFECT OF NITROGEN-FIXING BACTERIA ON GRAIN YIELD AND DEVELOPMENT OF FLOODED IRRIGATED RICE

2016 ◽  
Vol 29 (1) ◽  
pp. 11-17 ◽  
Author(s):  
AMAURI NELSON BEUTLER ◽  
GIOVANE MATIAS BURG ◽  
EVANDRO ADEMIR DEAK ◽  
MARCELO RAUL SCHMIDT ◽  
LEANDRO GALON
Keyword(s):  
Grain Yield ◽  
Rice Grain ◽  
Rice Seed ◽  
Nitrogen Fixing ◽  
Irrigated Rice ◽  
Randomized Design ◽  
Four Levels ◽  
Set Up ◽  
Nitrogen Rates ◽  
Liquid Inoculant

ABSTRACT: This study aimed at evaluating the effect of Azospirillum brasilense, a nitrogen-fixing bacterium, on flooded irrigated rice yield. Evaluations were carried out in a shaded nursery, with seedlings grown on an Alfisol. Were performed two sets of experiments. In the first, were carried out four experiments using the flooded rice cultivars INIA Olimar, Puitá Inta-CL, Br Irga 409 and Irga 424; these trials were set up as completely randomized design in a 5x4 factorial scheme, with four replications. Treatments consisted of five nitrogen rates (0, 40, 80, 120 and 160 kg ha-1) and four levels of liquid inoculant Ab-V5 and Ab-V6 - A. brasilense (0, 1, 2 and 4 times the manufacturer's recommendation) without seed treatment. In second set, were performed two experiments using the cultivars Puitá Inta-CL and Br Irga 409, arranged in the same design, but using a 4x2 factorial. In this set, treatments were composed of four levels of Ab-V5 and Ab-V6 - A. brasilense liquid inoculant (0, 1, 2 and 4 times the recommendation of 100 mL ha-1), using rice seeds with and without insecticide and fungicide treatment. Shoot dry matter, number of panicles, and rice grain yield per pot were the assessed variables. The results showed that rice seed inoculation with A. brasilense had no effects on rice grain yield of the cultivars INIA Olimar, Puitá Inta-CL, Br Irga 409 and Irga 424.

scholarly journals Viability of liquid medium-inoculation of Rhizobium etli in planting furrows with common bean

2018 ◽  
Vol 53 (3) ◽  
pp. 394-398
Author(s):  
Dâmiany Pádua Oliveira ◽  
Bruno Lima Soares ◽  
Fábio Aurélio Dias Martins ◽  
Leandro Alex Franceschini ◽  
Bruno Ewerton da Silveira Cardillo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Liquid Medium ◽  
Common Bean ◽  
Bean Seed ◽  
Rhizobium Etli ◽  
Application Method ◽  
Application Rates ◽  
Reduced Costs ◽  
Liquid Inoculant

Abstract: The objective of this work was to evaluate the viability of liquid medium inoculation of Rhizobium etli in the planting furrow and to certify the efficiency of its strain UFLA 02-100 as a potential inoculant for common bean (Phaseolus vulgaris). The treatments consisted of three application rates of liquid inoculant applied in the planting furrows or in common bean seed, besides two controls without inoculation. The inoculant contained 109 cells of Rhizobium etli mL-1. Regardless of the application method, the yield obtained with the inoculation was equivalent to that of N from urea; however, the application in the furrows, at 0.6 L ha-1, is more advantageous due to the operational practicality and reduced costs.

Positive effect of reduced aeration rate on growth and stereospecificity of dl-malic acid consumption by Azospirillum brasilense: Improving the shelf life of a liquid inoculant formulation

2015 ◽  
Vol 195 ◽  
pp. 74-81 ◽  
Author(s):  
Karen Carrasco-Espinosa ◽  
Ramsés I. García-Cabrera ◽  
Andrea Bedoya-López ◽  
Mauricio A. Trujillo-Roldán ◽  
Norma A. Valdez-Cruz
Keyword(s):  
Shelf Life ◽  
Malic Acid ◽  
Azospirillum Brasilense ◽  
Aeration Rate ◽  
Acid Consumption ◽  
Positive Effect ◽  
Inoculant Formulation ◽  
Liquid Inoculant

Rhizobium inoculant and seed-applied fungicide effects on field pea production

2002 ◽  
Vol 82 (4) ◽  
pp. 645-661 ◽  
Author(s):  
H. R. Kutcher ◽  
G. Lafond ◽  
A. M. Johnston ◽  
P. R. Miller ◽  
K. S. Gill ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Previous History ◽  
Field Pea ◽  
Negative Effects ◽  
Rhizobium Inoculation ◽  
Beneficial Effects ◽  
Positive Effects ◽  
Local Soil ◽  
Liquid Inoculant

Field pea has been shown to benefit from the use of rhizobium inoculation and seed-applied fungicides under intensive production. The objective of this research was to determine the effect of seed- or soil-applied rhizobium (Rhizobium leguminosarum bv. viciae) inoculants and seed-applied fungicides on field pea (Pisum sativum “Carneval”) production on fields with no previous history of the crop. The study was conducted at seven locations in Saskatchewan in each of 2 yr. Fungicide treatments were Apron FL (metalaxyl), Apron FL + Thiram 75WP (dithiocarbamate) and an untreated check in 1997 and a fourth treatment, Thiram 75WP, was added in 1998. Rhizobium treatments were seed-applied liquid inoculant, soil-applied granular inoculant and a non-inoculated check. Inoculation with rhizobium increased nodulation (5 of 10 sites), seed yield (6 of 13 sites), and protein content (3 of 8 sites), but occasionally appeared to reduce emergence (3 of 14 sites). Granular inoculant had more beneficial effects than the liquid inoculant. Fungicide treatments had few positive effects on production, and occasionally negative effects such as reduced emergence. Interaction effects were rare between seed-applied fungicide and seed-applied liquid or soil-applied granular rhizobium inoculants, which indicated compatibility of these products. Variability in the effects of inoculant types and fungicides suggests that responses are dependent on local soil and environmental conditions. Key words: Apron (metalaxyl), Thiram (dithiocarbamate), Pisum sativum, zero tillage

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