scholarly journals In vitro propagation of Vanda tricolor Lindl. var. suavis protocorm on media containing liquid organic fertilizer as a substitute for MS media

2021 ◽  
Vol 886 (1) ◽  
pp. 012006
Author(s):  
A I Latunra ◽  
M Tuwo ◽  
N Rezky
Keyword(s):  
Low Cost ◽  
Organic Fertilizer ◽  
Economic Value ◽  
Large Numbers ◽  
Important Solution ◽  
Number Of Leaves ◽  
Alternative Sources ◽  
Culture Technology ◽  
Number Of Shoots

Abstract Vanda orchids have a high economic value in the flower industry, so it is necessary to have seeds available at all times. Tissue culture technology offers an important solution to produce plants in large numbers, but it is very costly in media preparation. Hence, it is necessary to have low-cost options for the application of planting media. One way of doing this is to substitute the composition of Murashige and Skoog (MS) media with alternative sources that are more affordable. Liquid organic fertilizer contains macro, micro, and good nutrients for the growth of explants. This study used explants from the protocorm of Vanda tricolor Lindl orchid. var. suavis aged three months planted on media containing liquid organic fertilizer, namely Nasa, Bio88, and Fortune. The parameters observed were the number of shoots and the number of leaves in each treatment. The data were analyzed using the Kruskal-Wallis test at the 5% level, and if there was an effect, it was continued with the Mann-Whitney test. The results showed that liquid organic fertilizer gave different responses to the Vanda tricolor Lindl. var. suavis protocorm. Liquid organic fertilizer has a significant effect on the number of shoots and number of leaves.

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

scholarly journals PENGARUH JENIS AMELIORAN DAN DOSIS PUPUK SERBAGUNA (AGRODYKE) PADA PERTUMBUHAN RUMPUT GAJAH ODOT (Pennisetum Purpureum CV. Mott) DILAHAN GAMBUT

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuliatul Muslimah ◽  
Yusrizal Yusrizal ◽  
Imaniah Refkikan
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Organic Fertilizer ◽  
Leaf Length ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Type Factor ◽  
Number Of Shoots

Odot elephant grass is a tropical forage forage that is easily developed, has high production and can be used as animal feed.This study aims to determine the effect of ameliorant types and organic fertilizer (Agrodyke) doses on the growth of odot elephant grass (Pennisetum purpureum cv. Mott) on peatlands. This study used a 3x3 factorial randomized block design (RBD) with 3 replications consisting of: (1) ameliorant type factor (A) consisting of 3 levels, namely: A1 =dolomite lime, A2 = Shellfish shell, A3 = Eggshell. (2) Agrodyke fertilizer dose factor (D) Consists of 3 levels, namely: D0 = 0 gram / plant, D1 = 10 gram / plant, D2 = 20 gram / plant. Observation parameters are the number of leaves, number of shoots, leaf length, plant height. The results of this study indicate that the type of ameliorant did not significantly affect the number of leaves, number of shoots, leaf length, and plant height at 2 MST, 4 MST, and 6 MST. Versatile fertilizer (Agrodyke) dosage significantly affected the number of leaves and plant height at 6 MST. But no significant effect on the number of leaves, number of shoots and plant height in 2 MST and 4 MST. Keywords: Peat, Ameliorant Species, Odot Elephant Grass, Agrodyke.

scholarly journals PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN SETEK MIKRO KENTANG VARIETAS GRANOLA

2018 ◽  
Vol 3 (01) ◽  
pp. 26-33
Author(s):  
Efriana Jon
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Economic Value ◽  
Agricultural Crop ◽  
Micro Cutting ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Screen House ◽  
Number Of Shoots

Potato (Solanum tuberosum L) isan agricultural crop that has high economic value, it gets priority to be developed. This research at was conducted Screen House of Balai Benih Induk Kentang (BBIK) Kayu Aro subdistrict Kerinci regency.The purpose of this research was to know the effect of planting media On Micro Cutting Growth Of Granola Potato Variety (Solanum Tuberosum L). This study was used Completely Randomized Design (RAL) with 6 Treatment 10 Deuteronomy. The observed parameters were percentage of live micro cuttings potato, the high micro cuttings, number of shoots, numbr of leaves, and length of rootwere analyzed by the variance of investigation and followed by DNMRT advanced test at 5% level. The investigation result of the highest percentage (%)of micro cutting potato live was 90%. The High of micro cuttings, number of shoots and number of leaves of micro cuttings granola varieties was showed that no significant effect on the treatment given. Whereas for the root length of micro cuttings showed thatthere was a significantly different effect.

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals Propagation of Lily Arumsari using Generic Media through In Vitro Culture

2020 ◽  
Vol 11 (2) ◽  
pp. 140-148
Author(s):  
Ridho Kurniati ◽  
Fauziah Khairatunnisa ◽  
Reni Indrayanti
Keyword(s):  
Plant Growth Regulator ◽  
Low Cost ◽  
Ms Medium ◽  
Single Factor ◽  
Mass Propagation ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
The Cost

Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.

scholarly journals Improved in vitro rooting and acclimatization of “Violetta” Artichoke and “Green Globe” Artichoke

2021 ◽  
Vol 19 (1) ◽  
pp. 129-145
Author(s):  
Hoang Dac Khai ◽  
Nguyen Thi Nhu Mai ◽  
Hoang Le Lan Anh ◽  
Nguyen Nhu Minh Nguyet ◽  
Ho Viet Long ◽  
...  
Keyword(s):  
Large Scale ◽  
Economic Value ◽  
Shoot Multiplication ◽  
In Vitro Rooting ◽  
Globe Artichoke ◽  
Plantlet Production ◽  
Plantlet Quality ◽  
Number Of Shoots

Artichoke (Cynara scolymus L.), a medicinal plant with high economic value, contains high levels of phenolic compounds; especially cynarine, which plays an important role in preventing cancer, cardiovascular disease, osteoporosis, diabetes and neurodegeneration, etc. Currently, Artichoke micropropagation has achieved some success; however, the rooting efficiency and plantlet quality are still limited. In this study, improving the quality of Artichoke plantlet related to the shoot quality and suitable substrates in in vitro rooting stage was studied on “Violetta” Artichoke (VA) and “Green Globe” Artichoke (GA). The results showed that shoots (1.5 cm) cultured on MS medium supplemented 0.5 mg/L KIN were most suitable to shoot multiplication of VA with the number of shoots/explant (3.67 shoots), number of shoots ≥ 2 cm (3 shoots); while, 1.0 mg/L BA was suitable to shoot multiplication of GA (5.33 shoots; 5.00 shoots; respectively) after 4 weeks of culture. Besides, the in vitro rooting was improved using 8 g/L commercial agar for VA; meanwwhile, 3 g/L gelrite for GA. In addition, the nylon bag culture system (120 mm × 250 mm) has potential in plantlet production (15 plants/bag) and can be applied for large scale micropropagation. In addition, VA and GA plantlets derived from in vitro culture gave the good acclimatization, growth and development after 8, 12 and 20 weeks cultivating at the green house conditions.

scholarly journals Organic fertilizer on the in vitro cultivation of the Cattleya labiata orchid

2017 ◽  
Vol 2 (2) ◽  
pp. 62
Author(s):  
Rodrigo Thibes Hoshino ◽  
Ronan Carlos Colombo ◽  
Ana Paula Zandoná ◽  
Guilherme Augusto Cito Alves ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Organic Fertilizer ◽  
Dry Mass ◽  
In Vitro Cultivation ◽  
Shoot Height ◽  
Organic Products ◽  
Number Of Leaves

Propagation of in vitro plants through other culture media rather than the traditional ones has been widely researched, with satisfactory results. However, to increase the effectiveness of these media, the addition of organic products has been presented satisfactory results. Therefore, the objective of this work was to evaluate the influence of the FishFértil® organic fertilizer on the in vitro cultivation of the Brazilian orchid Cattleya labiata. Treatments included FishFértil® fertilizer concentrations of 0; 1; 2; 3; 4; 5 and 6 mL L-1 in a simplified culture medium. At 180 days, shoot height, number of leaves, leaf area, number of roots, root average length, shoot and root dry mass and the shoot: root ratio were evaluated. The experimental design was entirely randomized, with 10 replications, each one containing 10 plantules. Data were submitted to an analysis of variance and regression analysis, at 5% of significance. The FishFértil® organic fertilizer at the concentration of 6 mL L-1 promoted better plantule growth of Cattleya labiata, subcultivated in vitro, in a simplified culture medium.

scholarly journals Effect of Cytokines On The In Vitro of Sweet Kaffir Lime (Citrus hystrix Dc)

2020 ◽  
Vol 3 (2) ◽  
pp. 60
Author(s):  
Rd. Selvy Handayani ◽  
Ismadi Yunus ◽  
Nadia Tillah ◽  
Ira Handayani
Keyword(s):  
Tissue Culture ◽  
Shoot Growth ◽  
Plant Propagation ◽  
Number Of Leaves ◽  
Local Plants ◽  
Propagation Technique ◽  
High Quality Seed ◽  
The Right ◽  
Culture Technology

Sweet kaffir lime (Citrus hystrix Dc), also called "Boh Kruet Mameh" is one of Aceh's local plants, which is currently very difficult to obtain. Sweet kaffir lime is not like the ordinary kaffir lime, which tastes sour. The fruit of sweet kaffir lime has a larger size than the regular kaffir lime, sweet and sour taste, aromatic and fresh, which can be consumed as a fruit table. The problem of developing the propagation technique of sweet kaffir lime plants is the seeds' condition, which are conventionally difficult to germinate and limited in number, making it difficult to get a high-quality seed. Thus, to overcome these problems, suitable solutions to plant propagation techniques should be sought. As for the right plant propagation technique is tissue culture technology or in vitro propagation technique. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. The research was conducted from February to April 2018. This research used a single randomized complete design (CRD) with ten replications. The treatment is a combination of the basic media, which consists of MS0 (MS media without BAP) and MS1 (MS + BAP 4 ppm). The result showed that the adduction of 4 ppm BAP in MS media influenced the in vitro sweet kaffir lime seeds' growth. Sweet kaffir lime seeds that were given 4 ppm BAP grew buds faster with more percentage of shoot growth, the number of shoots, and a number of leaves than the seeds without BAP adduction.

Regeneration of Medicinal Plant Paederia foetida through Direct Organogenesis using Nodal Explants

2021 ◽  
Vol 24 (1) ◽  
pp. 89-98
Author(s):  
SH Binto ◽  
JU Ahmed ◽  
TK Ghosh
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Surface Sterilization ◽  
Paederia Foetida ◽  
Number Of Leaves ◽  
Number Of Shoots

Chinese fever vine (Paederia foetida L.), a valuable medicinal plant has been greatly utilized in therapeutic purposes throughout the world. Since conventional propagation techniques of P. foetida are very slow, inefficient and cannot cope with the increasing demand, in-vitro regeneration through tissue culture could be an alternative means of rapid propagation. Therefore, the efforts were made to develop a suitable protocol through direct organogenesis of P. foetida. After surface sterilization, the nodal explants were cultured in Murashigue and Skoog (MS) medium and MS medium supplemented with different concentrations and combinations of plant growth regulators. MS medium supplemented with 6-benzylaminopurine; BAP (2.0 mg L-1) produced the maximum number of shoots; 4.40 ± 0.98 and 5.40±1.12 after 15 and 30 days of culture respectively. The number of shoots gained by 15 days was found to be the highest; 1.20±0.80 at BAP (4.0 mg L-1) followed by 1.00±0.55 at BAP (2.0 mg L-1). Although the combination of BAP + Kinetin (2 mg L-1 +2 mg L-1) showed the highest shoot growth (3.40 ± 1.08 cm) by 15 days, sole application of BAP (2.0 mg L-1) or Kn (0.5, 1.0, 2.0 and 3.0 mg L-1) showed similar responses. BAP (2.0 mg L-1) showed the best responses for developing the highest number of leaves; 18.60 ± 2.42 and 29.20 ± 2.73 respectively after 15 and 30 days of culture. Similarly, development of the maximum number of leaves (10.60 ± 0.68) was reported by 15 days at BAP (2.0 mg L-1). Rooting was significantly induced in indole-3-acetic acid (IAA) supplemented to 1/2 strength MS medium as compared to control (only ½ strength MS medium). The best performance of rooting was observed by 0.5 mg L-1 IAA which produced average 4.33 roots per shoot after 21 days of culture. The regenerated plants showed similar morphology to the mother plants. Thus, a suitable protocol for successful multiplication of P. foetida in vitro was established using nodal explants. Ann. Bangladesh Agric. (2020) 24(1) : 88-98

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