scholarly journals Effect of Cytokines On The In Vitro of Sweet Kaffir Lime (Citrus hystrix Dc)

2020 ◽  
Vol 3 (2) ◽  
pp. 60
Author(s):  
Rd. Selvy Handayani ◽  
Ismadi Yunus ◽  
Nadia Tillah ◽  
Ira Handayani
Keyword(s):  
Tissue Culture ◽  
Shoot Growth ◽  
Plant Propagation ◽  
Number Of Leaves ◽  
Local Plants ◽  
Propagation Technique ◽  
High Quality Seed ◽  
The Right ◽  
Culture Technology

Sweet kaffir lime (Citrus hystrix Dc), also called "Boh Kruet Mameh" is one of Aceh's local plants, which is currently very difficult to obtain. Sweet kaffir lime is not like the ordinary kaffir lime, which tastes sour. The fruit of sweet kaffir lime has a larger size than the regular kaffir lime, sweet and sour taste, aromatic and fresh, which can be consumed as a fruit table. The problem of developing the propagation technique of sweet kaffir lime plants is the seeds' condition, which are conventionally difficult to germinate and limited in number, making it difficult to get a high-quality seed. Thus, to overcome these problems, suitable solutions to plant propagation techniques should be sought. As for the right plant propagation technique is tissue culture technology or in vitro propagation technique. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. The research was conducted from February to April 2018. This research used a single randomized complete design (CRD) with ten replications. The treatment is a combination of the basic media, which consists of MS0 (MS media without BAP) and MS1 (MS + BAP 4 ppm). The result showed that the adduction of 4 ppm BAP in MS media influenced the in vitro sweet kaffir lime seeds' growth. Sweet kaffir lime seeds that were given 4 ppm BAP grew buds faster with more percentage of shoot growth, the number of shoots, and a number of leaves than the seeds without BAP adduction.

scholarly journals Respon Kunir Putih (Kaempferia rotunda) terhadap Pemberian IBA dan BAP pada Kultur In Vitro

2016 ◽  
Vol 18 (2) ◽  
pp. 44
Author(s):  
Ahmad Yunus ◽  
Muji Rahayu ◽  
Samanhudi Samanhudi ◽  
Bambang Pujiasmanto ◽  
Himawan Joko Riswanda
Keyword(s):  
Tissue Culture ◽  
Gastrointestinal Disorders ◽  
High Quality ◽  
Plant Propagation ◽  
Large Numbers ◽  
Plant Growth Promoting ◽  
Main Observation ◽  
Growth Promoting ◽  
High Quality Seed

<p><em>Kaempferia rotunda </em>that long ago been used to treat gastrointestinal disorders and also treat cancer, has<em> </em>promising opportunities to cultivate. There are several obstacles in cultivation today. Plant propagation by <em>in</em> <em>vitro </em>can be one solution.<em> </em>Basic media and plant growth promoting substance usage is an important factor which<em> </em>has to be concerned. Research aiming to produce high quality seed and in large numbers. Research has been carried out in Tissue Culture Laboratory, Agriculture Faculty, Sebelas Maret University, Surakarta, during July 2014 to August 2015. This research was used MS (Murashige and Skoog) media with combination treatment of each IBA and BAP concentration of 0, 1, 2, 3, and 4 ppm. The number of shoots that appear on the explant is the main observation variables. The results showed IBA and BAP are likely to affect the appearance of buds as much as 2 pieces in each explant at 0 ppm IBA treatment.</p>

scholarly journals In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.)

2017 ◽  
Vol 2 (2) ◽  
pp. 37-42
Author(s):  
Samanhudi Samanhudi ◽  
Ahmad Yunus ◽  
Bambang Pujiasmanto ◽  
Anindya Saras
Keyword(s):  
In Vitro Propagation ◽  
Raw Materials ◽  
Root Number ◽  
Natural Habitats ◽  
Plant Propagation ◽  
Hair Roots ◽  
Number Of Leaves ◽  
Curcuma Xanthorrhiza ◽  
Propagation Technique

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

scholarly journals Kajian Penambahan BahanOrganik Pada Media Tanam VW Pada Organogenesis AnggrekDendrobium SecaraIn Vitro

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Siti Rahmah ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Tissue Culture ◽  
Organic Matter ◽  
Optimization Technique ◽  
Media Composition ◽  
Growth Optimization ◽  
The World ◽  
The Media ◽  
Number Of Leaves ◽  
Bean Sprouts

In vitro tissue culture is a growth optimization technique of Dendrobium orchid with according to media composition. Nutritions in the media are important for dendrobium orchid. Dendrobium orchid  include plant from orchidaceae family its spread throughout the world like indonesia. Its features are easily planted, intersest is continuous and varied, easily assembled, the flower crown is not easy to fall and wither. Research aimed at obtaining media compositions that are easily available and able to fulfill the needs of orchid plants. The research was conducted using descriptive methods to compare different trearment; Vacin & Went and VW media with adding organic matter; extract bean sprouts, potato extrac, and water coconut; wich is conducted for eight weeks after planting. The result of addition organic matter on VW media was different toward organogenesis of orchid. The average number of shoots is 1.8; the number of leaves average of 6.8 and the number of roots average of 3.6 formed from two until eight weeks after culture.Keywords: tissue culture, growing media, Dendrobium orchid, organogenesis.ABSTRAKKultur jaringan in vitro adalah salah satu teknik optimalisasi pada pertumbuhan tanaman angrek Dendrobium dengan menyesuaikan komposisi medianya. Nutrisi yang terdapat di dalam media sangat penting bagi pertumbuhan anggrek. Anggrek Dendrobium termasuk tanaman dari keluarga Orchidaceae yang penyebarannya sampai ke pelosok dunia seperti Indonesia. Keistimewaanya mudah ditanam, bunganya terus-menerus dan bermacam-macam, mudah disusun, serta mahkota bunga tidak mudah jatuh dan layu. Penelitian yang bertujuan untuk mendapatkan komposisi media yang mudah didapat dan mampu memenuhi kebutuhan tanaman anggrek. Penelitian dilakukan menggunakan metode deskriptif untuk membandingkan perlakuan media yang berbeda yaitu media Vacin & Went, dan VW dengan penambahan bahan organik; ekstrak tauge kacang hijau, ekstrak kentang, dan air kelapa muda; yang dilakukan selama delapan minggu setelah tanam. Hasil penambahan bahan organik pada media VW berbeda terhadap organogenesis eksplan anggrek. Jumlah tunas rata-rata 1,8; Jumlah daun rata-rata 6,8 dan jumlah akar rata-rata 3,6 yang terbentuk dari dua minggu setelah kultur (MSK) sampai minggu terakhir pengamatan delapan MSK.Kata kunci: kultur jaringan, media tanam, angrek Dendrobium,organogenesis.

scholarly journals COMMERCIAL MICROPROPAGATION OF Syringa SPECIES AND HYBRIDS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1096f-1096
Author(s):  
Lynne Edick Caton ◽  
Steve McCulloch
Keyword(s):  
Tissue Culture ◽  
Quantitative Data ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Phenotypic Differences ◽  
Commercial Micropropagation

Briggs Nursery has micropropagated lilacs commercially since 1982. Presently we are producing more than 30 species and hybrids and have observed that media requirements vary significantly. In this study 5 lilacs representing a range of germtypic and phenotypic differences were examined to optimize cytokinin concentrations for shoot growth in vitro. Lilacs were grown on MS salts with BA, 2iP, and zeatin used individually and in combination. Plants were subculture 3 times, at 6 week intervals, at the conclusion of which quantitative data was taken with respect to shoot multiplication, elongation, and quality. These factors help determine the commercial practicality of producing lilacs through tissue culture.

scholarly journals Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fast Time ◽  
Endogenous Auxin ◽  
Seed Technology ◽  
Randomized Design ◽  
Process Plants ◽  
Number Of Leaves ◽  
Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

Perbandingan Metode Sterilisasi Untuk Perbanyakan Rubus rosifolius Secara in Vitro

2021 ◽  
Vol 14 (1) ◽  
pp. 127-137
Author(s):  
Muhammad Imam Surya ◽  
Lily Ismaini
Keyword(s):  
Tissue Culture ◽  
Ascorbic Acid ◽  
In Vitro Propagation ◽  
Povidone Iodine ◽  
Tween 80 ◽  
Botanical Garden ◽  
The Other ◽  
Fruit Crops ◽  
Plant Propagation

AbstrakRubus rosifolius adalah salah satu jenis rasberi liar yang memiliki potensi cukup tinggi untuk dikembangkan sebagai tanaman buah. Selain itu, metode perbanyakan tanaman merupakan salah satu faktor yang berpengaruh dalam pembudidayaan. Lebih lanjut, informasi terkait upaya perbanyakan R. rosifolius secara in vitro masih sangat terbatas. Percobaan ini ditujukan untuk mengetahui metode sterilisasi yang tepat pada eksplan R. rosifolius. Sebanyak 17 metode sterilisasi telah diujicobakan di Laboratorium Kultur Jaringan BKT Kebun Raya Cibodas-LIPI. Bahan sterilisasi yang digunakan, yaitu detergen, tween 80, bakterisida, fungisida, clorox/pemutih (NaClO), alkohol 70%, larutan Plant Preservative Mixture (PPM), vitamin C/asam askorbat, dan povidone iodine/antiseptik. Hasil percobaan menunjukkan bahwa metode sembilan merupakan metode sterilisasi yang cukup optimum untuk sterilisasi eksplan R. rosifolius. Metode sembilan mampu menghambat munculnya mikroorganisme endofitik hingga 8 hari dan tidak menyebabkan warna eksplan menjadi cokelat/browning. Tahapan sterilisasi pada metode sembilan meliputi pencucian dengan detergen, perendaman dengan bakterisida + fungisida selama +30 menit, perendaman dengan clorox 10% + tween 80 selama +15 menit, pencucian dengan larutan PPM selama +15 menit.  AbstractRubus rosifolius is one of the species from wild raspberries, which is has high potential to develop as a fruit crops. In the other hand, the technique of plant propagation became an important factor for cultivation. Moreover, the information related to the in vitro propagation of R. rosifolius is very limited. This experiment was aimed to determine the best method to sterilize an explants of R. rosifolius. About 17 methods of sterilization have been tried in the laboratorium of tissue culture at Cibodas Botanical Garden-Indonesian Institute of Sciences. The combination of detergent, tween 80, bactericide, fungicide, sodium hypochlorite (NaClO), alcohol 70%, plant preservative mixture (PPM), ascorbic acid, and povidone iodine were used during the experiment. The results show that the method of sterilization number nine could be inhibit the emergence of endophytic organisms for eight days and keep an explant in green with a little brownish compared by the others methods. The method of sterilization number nine was consist of several steps i.e. wash by detergent, soak in bactericide + fungicide for +30 minutes, soak in sodium hypoclorite 10% + tween 80 for +15 minutes, wash by PPM solution for +15 minutes.

scholarly journals Pertumbuhan Tunas Pisang Barangan (Musa Acuminata Colla.) akibat Pemberian Benzyl Amino Purin dan Arang Aktif secara In Vitro.

2019 ◽  
Vol 4 (1) ◽  
pp. 73-90
Author(s):  
Mulia Saputri ◽  
Marai Rahmawati ◽  
Elly Kesumawati
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Shoot Growth ◽  
Growth Time ◽  
Shoot Height ◽  
High Productivity ◽  
Horticultural Crops ◽  
Randomized Design ◽  
And Control

Pertumbuhan Tunas Pisang Barangan Akibat Pemberian Benzyl AminoPurin dan Arang Aktif secara In Vitro Accretion of Barangan Banana Shoot Effect of  (BAP)  and Activated Charcoal Explant by In VitroAbstrak. Banana as a superior product of horticultural crops, has not achieved high productivity and has several obstacles in its multiplication. Tissue culture is one solution to overcome this problem. This study aims to determine the composition of PGRBenzil Amino Purine (BAP) and activated charcoal that are appropriate in the multiplication of barangan banana shoots. This research was conducted at the Tissue Culture Laboratory Faculty of Agriculture Syiah Kuala University, Darussalam Banda Aceh. The design used was a Completely Randomized Design (CRD) with two treatment factors. The first factor is BAP concentration consisting of 3 levels, namely 4 mg/L, 6 mg /L, and 8 mg/L. The second factor is activated charcoal concentration consisting of 3 levels, namely control, 1 g/L and 2 g/L. In this study, from 9 treatment combinations, only 4 treatment combinations were not contaminated. Of the 4 treatment combinations the combination of BAP concentration of 6 mg/L and control (without activated charcoal) showed the fastest shoot growth time of 29 days after multiplication, the most shoot growth was 6 shoots and the average shoot height was 15.9 mm

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