scholarly journals The effect of probiotics on digestive enzyme activity during larvae and juvenile stage of Yellow Fin Tuna (Thunnus albacares)

2021 ◽  
Vol 890 (1) ◽  
pp. 012019
Author(s):  
Haryanti ◽  
Gunawan ◽  
A Setiadi ◽  
S B M Sembiring ◽  
I G N Permana ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Target Genes ◽  
Digestive Enzyme ◽  
Rna Isolation ◽  
Enzyme Synthesis ◽  
Larval Rearing ◽  
Thunnus Albacares ◽  
Bacterial Strains ◽  
Fish Samples

Abstract Yellow fin tuna fry production technology is not successful yet. This was assumed related to the biological and physiological characteristics of the fish. The physiological approach to regulating digestion through probiotics is needs to be studied. The aim of this study was to examine the effect of probiotics bacterial strains of the enzymatic activity in digestion system for the larvae to juvenile stages of yellow fin tuna, Thunnus albacares. The experiment was initiated by culturing 3 probiotic isolates (Bacillus subtilis strain TA-1, Bacillus amyloliquefaciens strain TN-2, Bacillus subtilis strain TO-4) and apply to the larval rearing of yellow fin tuna. In the experiment the treatments applied were supplemented probiotics and without probiotic, each treatment was held with 2 replications. Fish samples were collected every day and analyze the gene expression profile associated with digestion enzyme synthesis by total RNA isolation and cDNA synthesis followed analysis of its by using the RT –qPCR method. The results showed that the growth response of T. albacares tuna larvae reared with probiotics tended to be faster (P<0.05) than the control (without probiotics). The supplementation of probiotic could improved the expressed digestibility of the target genes associated with enzymatic synthesized. Amylase enzymatic synthesized increase 200-1200 times in larvae 10 –17 Day After Hatch (DAH), while in the lipase enzymatic activity started with 13 DAH ( 25 time) and 16 – 21 DAH by 150-300 times. In trypsin the highest enzymatic activity was at 16 DAH, which was 200 times. While in control, the expression of enzymatic synthesis of amylase, lipase, and trypsin was relatively low.

scholarly journals Epiphytic bet-mannanase producing bacterial strains

2021 ◽  
Vol 37 ◽  
pp. 00125
Author(s):  
Ekaterina Epova ◽  
Svetlana Petrova ◽  
Elena Trubnikova ◽  
Maria Danilova
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Bacterial Species ◽  
Maximum Activity ◽  
Plantago Major ◽  
Single Source ◽  
Artemisia Vulgaris ◽  
Bacterial Strains ◽  
Pcr Cloning ◽  
Herbal Species

Dry remains of the herbal species of the plantain (Plantago major), the wormwood (Artemisia vulgaris) and the reed grass (Calamagrostis acutiflora) were used as a natural source for isolation of β- mannanase producing strains. They were isolated by using the carob gum as a single source of carbon and energy. Each chosen plant species was found to be colonized with a single dominant epiphytic group of microorganism, although the plants had been collected in the same location. Bacillus circulans was only found in P. major, Bacillus subtilis on A. vulgaris, whereas Pantoea sp. was found in C. acutiflora. Identification of the taxonomy affiliation of the isolated β-mannanase producers allowed using the formerly proposed primers for PCR cloning of β-mannanase genes previously occurred in the respective bacterial species. This approach let us cloning 330 bp fragment of β-mannanase genes from B. circulans and B. subtilis and 1000 bp fragment of β-mannanase gene from Pantoea sp. Testing the enzymatic activity of the isolated strains by staining the carob gum hydrolysis zones on the plates with Congo Red was carried out. As a result, the maximum activity was found in Pantoea sp.

scholarly journals Feeding Artemia nauplii enriched with the probiotic bacterium Bacillus subtilis improved growth performance, survival and digestive enzyme activity of Clarias batrachus (Linnaeus, 1758) larvae

2019 ◽  
Vol 66 (2) ◽  
Author(s):  
S.P. Kamble ◽  
A.K. Sahu ◽  
S. Mohanty ◽  
S.K. Sahoo ◽  
K. Murmu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Digestive Enzyme ◽  
Bacterial Count ◽  
Probiotic Bacterium ◽  
Clarias Batrachus ◽  
Digestive Enzyme Activity ◽  
Control Group ◽  
Larval Rearing ◽  
Artemia Nauplii

Clarias batrachus (Linnaeus, 1758) larvae were subjected to different modes of probiotic (Bacillus subtilis) administration such as:  T1: feeding Artemia nauplii enriched with B. subtilis; T2: feeding enriched Artemianauplii + addition of B. subtilis in water  and T3: addition of B. subtilis in water alone, for a period of 14 days. A control group (T0) with no probiotic administration was also maintained. On termination of the experiment, probiotic supplemented through enriched Artemia nauplii along with addition in  water (T2) yielded significantly (p<0.05) higher length,  weight, weight gain, specific growth rate and survival compared to control (T0) and T3 groups. All these parameters did not differ significantly (p>0.05) between T1 and T2. Similar trend was observed in the case of total gut bacterial count and digestive enzyme activity. The highest (p<0.05) digestive enzyme activity of protease, lipase and amylase   were recorded in T2, while no significant (p>0.05) difference was observed between T2 and T1. The present study reiterated the advantage of B. subtilis, and its administration through enriched Artemia nauplii which could be a promising supplementation mode during C. batrachus larval rearing.

THE EFFECT OF BACTERIAL STRAINS ON THE ENZYMATIC ACTIVITY OF SOILS IN IMPACT ZONE OF ATAMAN LAKE

2020 ◽  
Author(s):  
V.V. Zinchenko ◽  
◽  
E.S Fedorenko ◽  
A.V Gorovtsov ◽  
T.M Minkina ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Dehydrogenase Activity ◽  
Contaminated Soil ◽  
Model Experiment ◽  
Impact Zone ◽  
Bacterial Strains ◽  
The Impact

As a result of the model experiment, an increase in the enzymatic activity of meadow chernozem of the impact zone of Ataman Lake with the introduction of a strains mixture of metal-resistant microorganisms into the soil was established. The experiment has shown that the application of bacterial strains increases the dehydrogenase activity of contaminated soil by 51.8% compared to the variant without remediation

Enzymatic activity of strains of bacteria Bacillus subtilis isolated from frozen soils

2020 ◽  
Vol 1 (1) ◽  
pp. 73-79
Author(s):  
M.P. Scriabina ◽  
◽  
A.M. Stepanova ◽  
N.P. Tarabukina ◽  
M.P. Neustroev ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Frozen Soils

scholarly journals Effects of Bacillus subtilis on growth performance, serum parameters, digestive enzyme, intestinal morphology, and colonic microbiota in piglets

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo Deng ◽  
Jie Wu ◽  
Xiaohui Li ◽  
Cheng Zhang ◽  
Xiaoming Men ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Digestive Enzyme ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Promising Alternative ◽  
Serum Parameters ◽  
Colonic Microbiota ◽  
Diet Supplementation

AbstractThe present study was conducted to investigate effects of Bacillus subtilis on growth performance, serum parameters, digestive enzymes, intestinal morphology, and colonic microbiota in piglets. A total of 72 piglets were weighed and randomly allotted into three treatments (four replication pens per treatment with six piglets/pen) for a 28-day experiment. The dietary treatments were as follows: basal diet (control group, CTR), basal diet supplementation with antibiotic (antibiotic group, ABT), and basal diet supplementation with 0.1% Bacillus subtilis (probiotic group, PBT). The average daily gain of body weight increased in both the ABT and PBT groups, and dietary antibiotics decreased the feed:gain ratio (F:G), as compared to the CTR group (P < 0.05). Both ABT and PBT piglets had increased serum triglycerides and lipase, amylase, maltase activities and villus height:crypt depth ratio (V/C) in ileum (P < 0.05). The PBT group also showed an increase in serum glucose and villus height in the ileum (P < 0.05). Dietary antibiotics increased Lactobacillus johnsonii, as compared to the CTR group, but decreased bacterial diversity and increased Escherichia coli, as compared to the PBT group (P < 0.05). Piglets dietary with B. subtilis modulated the microbiota by increasing the abundance of Firmicutes (L. johnsonii, L. reuteri) and decreasing the abundance of E. coli, as compared to the control group (P < 0.05). These results indicate that dietary of B. subtilis improves growth performance and intestinal health and can be a promising alternative to antibiotics in piglets diet.

PRODUCTION OF CYTASES ACTIVE ON BARLEY GUM BY BACTERIA OF THE GENUS BACILLUS

1953 ◽  
Vol 31 (1) ◽  
pp. 28-32 ◽  
Author(s):  
A. C. Blackwood
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Nitrogen Source ◽  
Freeze Drying ◽  
Shake Flask ◽  
Sole Nitrogen Source ◽  
Genus Bacillus ◽  
Original Activity ◽  
Bacterial Cultures ◽  
Bacillus Genus

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.

scholarly journals Intracellular serine proteinase of Bacillus subtilis strain Marburg 168. Comparison with the homologous enzyme from Bacillus subtilis strain A-50

1979 ◽  
Vol 179 (2) ◽  
pp. 333-339 ◽  
Author(s):  
A Y Strongin ◽  
D I Gorodetsky ◽  
I A Kuznetsova ◽  
V V Yanonis ◽  
Z T Abramov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Terminal Sequence ◽  
Gramicidin S ◽  
Intracellular Enzymes ◽  
Sepharose 4B ◽  
Homologous Enzyme ◽  
Strict Selection

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

scholarly journals Regulation of Poly(ADP-ribose) Polymerase-1-dependent Gene Expression through Promoter-directed Recruitment of a Nuclear NAD+ Synthase

2012 ◽  
Vol 287 (15) ◽  
pp. 12405-12416 ◽  
Author(s):  
Tong Zhang ◽  
Jhoanna G. Berrocal ◽  
Jie Yao ◽  
Michelle E. DuMond ◽  
Raga Krishnakumar ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Target Gene ◽  
Target Genes ◽  
Enzymatic Activities ◽  
Gene Promoters ◽  
Protein Coding ◽  
Photon Excitation ◽  
Gene Sets ◽  
Cellular Compartments ◽  
Parp 1

NMNAT-1 and PARP-1, two key enzymes in the NAD+ metabolic pathway, localize to the nucleus where integration of their enzymatic activities has the potential to control a variety of nuclear processes. Using a variety of biochemical, molecular, cell-based, and genomic assays, we show that NMNAT-1 and PARP-1 physically and functionally interact at target gene promoters in MCF-7 cells. Specifically, we show that PARP-1 recruits NMNAT-1 to promoters where it produces NAD+ to support PARP-1 catalytic activity, but also enhances the enzymatic activity of PARP-1 independently of NAD+ production. Furthermore, using two-photon excitation microscopy, we show that NMNAT-1 catalyzes the production of NAD+ in a nuclear pool that may be distinct from other cellular compartments. In expression microarray experiments, depletion of NMNAT-1 or PARP-1 alters the expression of about 200 protein-coding genes each, with about 10% overlap between the two gene sets. NMNAT-1 enzymatic activity is required for PARP-1-dependent poly(ADP-ribosyl)ation at the promoters of commonly regulated target genes, as well as the expression of those target genes. Collectively, our studies link the enzymatic activities of NMNAT-1 and PARP-1 to the regulation of a set of common target genes through functional interactions at target gene promoters.

scholarly journals Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus)

2018 ◽  
Vol 6 (2) ◽  
pp. 500-508
Author(s):  
Julie Ann A. Arcales ◽  
Garner Algo L.Alolod
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzymatic Activity ◽  
Antimicrobial Property ◽  
Food Products ◽  
The Philippines ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Isolation And Characterization

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

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