scholarly journals Characteristics of the photosynthetic apparatus of Pinus sylvestris L. and Larix sibirica Ledeb. from different locations of the Irkutsk region (Eastern Siberia)

2021 ◽  
Vol 908 (1) ◽  
pp. 012026
Author(s):  
M V Oskorbina ◽  
O V Kalugina ◽  
L V Afanasyeva ◽  
T M Kharpukhaeva

Abstract The characteristics of the photosynthetic apparatus of coniferous growing in various forest types and climatic conditions of the Irkutsk region (Taishet, Bratsk, Shelekhov, Irkutsk districts) have been explored. The content of photosynthetic pigments, total, free, and bound water in the needles of P. sylvestris and L. sibirica were studied. Using a pulse fluorimeter PAM 2500 (Waltz, Germany), the chlorophyll fluorescence in needles was measured based on such induction curve indices as Fv/Fm, Y(II), NPQ, and ETR. According to the chlorophyll fluorescence induction curve parameters of Scots pine and Siberian larch needles growing in different forest growth conditions, the most effective potential of primary photochemical processes in PSII was observed in Scots pine. While studying the pigment fund of Scots pine and Siberian larch needles, it was found that the highest values of the content of chlorophyll (Chl) a, b, and carotenoids were observed in P. sylvestris and L. sibirica growing in the climatic and forest growth conditions of the Shelekhov district. It is assumed that the revealed characteristics of the parameters reflect the processes of adaptation of the photosynthetic apparatus to various environmental conditions during vegetation development, as well as its high plasticity in different climatic growth conditions.

Author(s):  
M. Y. Mazura ◽  
N. V. Miroshnyk ◽  
I. K. Teslenko

The informativeness is established of the method of induction of chlorophyll fluorescence for bioindication in the conditions of anthropogenic influence on the example of T. officinale plants, by the parameters: F0, Fm, Fst, Fv, Fv/Fm та k1, k2. The main parameters of the induction curve can be used as test indicators to determine the resistance of plants to adverse factors. Changes in these indicators characterize the processes associated with the influence of the environment on the course of both light and dark phases of photosynthetic processes in chloroplasts. The photosensitivity of T. offic- inale leaves, which indicates the physiological state of plants, decreases with increasing adverse external influences. It is established that the plants of T. officinale have the maximum intensity of photosynthesis, which grow in more favorable ecological conditions control. Therefore, the use of the device «Floratest» to determine the condition of plants at different levels of anthropogenic load is promising and requires further research.


2020 ◽  
Vol 31 (1) ◽  
pp. 11-15
Author(s):  
K. K. Holoborodko ◽  
A. A. Alekseeva ◽  
O. V. Seliutina ◽  
V. A. Gorban

The article presents the results of studies on Cameraria ohridella Deschka & Dimič, 1986 feeding on the state of the photosynthetic apparatus in Aesculus hippocastanum Linnaeus, 1753. The studies were carried out in June 2019 (during the life of C. ohridella first generation) on the territory of the Botanical Garden of the Oles Honchar Dnipro National University. Photosynthesis is one of the processes most susceptible to stress factors; important information on state of the photosynthetic apparatus in plants under influence of various abiotic and biotic factors can be obtained by fluorescence analysis. Chlorophyll fluorescence is an indicator that allow to investigate a behavior of photochemical reactions related to system II (PSII) in living objects as an indicator the most sensitive to effect of environmental factors. One of the effective methods for plant state monitoring is a technique on chlorophyll fluorescence induction (CFI) measurement, which at the present stage can be introduced due to the use of biosensors. The objective of our study was to establish characteristics of C. ohridella vital activity on the photosynthesis process in A. hippocastanum trees using CFI evaluation technique. To diagnose photosynthesis violation of native chlorophyll in a living leaf of A. hippocastanum, we used a portable fluorometer “Floratest” (spectral range for measuring fluorescence intensity 670 to 770 nm) developed by V. M. Glushkov Institute of Cybernetics of National Academy of Sciences of Ukraine. The studies were conducted using alive A. hippocastanum leaves not damaged by C. ohridella and, accordingly, the damaged ones. Analysis of the data obtained showed that the worm feeding affects all CFI parameters (initial CFI value (non-damaged leaf: 582 relative units, damaged: 264); “plateau” CFI value (1460 to 722); maximum CFI (1890 to 940) and stationary CFI value after light adaptation of the leaf (1530 to 746)). Our studies have shown that CFI technique using “Floratest” apparatus allow express determining the general condition of the plant by assessing the main process of vital activity, namely photosynthesis. Analysis of chlorophyll fluorescence parameters is a powerful and effective tool for determining the phytophage effect on the plant.


2021 ◽  
Vol 12 (2) ◽  
pp. 346-352
Author(s):  
K. K. Holoborodko ◽  
O. V. Seliutina ◽  
I. A. Ivanko ◽  
A. A. Alexeyeva ◽  
M. V. Shulman ◽  
...  

The complex of invasive species of phytophagous insects that can adapt to novel living conditions is constantly increasing. The ecological effect of their vital activity in the new environment is almost impossible to predict. As a result, invasions undesirable in the economic sense are often observed. The horse -chestnut leaf miner (Cameraria ohridella Deschka & Dimič, 1986, Gracillariidae) is one of these problematic invader species in the introduced range of the horse chestnut (Aesculus hippocastanum (Linnaeus, 1753), Sapindaceae). We studied the effect of C. ohridella on the state of the photosynthetic apparatus in Ae. hippocastanum leaves. Photosynthesis is the one of the processes most vulnerable to stress factors, so information about the state of photosynthetic apparatus in a plant under the influence of phytophage feeding obtained with fluorescence analysis can be significant. The feeding effect of C. ohridella caterpillars on the functional state and activity of Ae. hippocastanum photosynthetic apparatus was studied. We studied critical parameters of chlorophyll fluorescence induction reflecting the effect of a single C. ohridella generation (feeding the caterpillar for the five stages of this species’ development) on the functional links of the photosystem II in Ae. hippocastanum leaves. The data obtained show a decrease in PS II quantum efficiency (inhibition of photosynthetic activity) in the leaves from different parts of the crown; it suggests the destructive effect of C. ohridella caterpillar feeding on Ae. hippocastanum photosynthetic apparatus. Values of all key parameters of chlorophyll fluorescence indication evidence inhibition of photophysical and photochemical processes of photosynthesis and impaired coherence of Calvin cycle reactions. Study of the feeding effect of C. ohridella caterpillars on the efficiency of the main enzyme in the Calvin cycle (which closely correlates with the coefficient of fluorescence induction that characterizes the efficiency of dark photosynthetic processes) showed a significant decrease in its activity in the leaves of both the illuminated and shaded parts of the crown. Our study has shown that the method of chlorophyll fluorescence induction allows one to determine the general state of a plant in an express regime by evaluating the main process of plant life as photosynthesis. Analysis of chlorophyll fluorescence parameters is a powerful and effective tool for determining the effect of phytophages on the plant body. The obtained data allow us to apply the method of analyzing chlorophyll fluorescence induction in practice to establish the physiological state of tree flora in forests and garden farms.


Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1154
Author(s):  
Valentina Spanic ◽  
Selma Mlinaric ◽  
Zvonimir Zdunic ◽  
Zorana Katanic

Wheat is one of the main cereal crops for ensuring food supply. Thus, increasing grain yield is a major target for plant breeders, where insights into wheat productivity can be gained by studying the activity of the components of photosynthetic apparatus. The objectives of this study were to evaluate the agronomical performance of three winter wheat varieties and test photosynthetic efficiency over two different locations. Chlorophyll fluorescence was used to evaluate the maximum quantum yield of photosystem II (PSII) (TR0/ABS) and performance index on absorption basis (PIabs) of flag leaves and glumes of heads at the flowering stage until the mid-senescence stage. The grain yield of all varieties on average was significantly higher at Osijek compared to Tovarnik. Variety Tika Taka exhibited the highest yield reduction (27.1%) at Tovarnik compared to Osijek, followed by El Nino (20.5%) and Vulkan (18.7%), respectively. A higher amount of precipitation in June at Tovarnik provoked higher Fusarium head blight disease intensity, which could be seen as the bleaching of plant heads at the plots and resulted in an earlier decrease in photosynthetic activity. Therefore, earlier senescence and contracted grain fill duration could occur.


2014 ◽  
Vol 25 (1-2) ◽  
pp. 92-100
Author(s):  
P. M. Bujanov

The town of Oleshky, renamed as Tsiurupinsk in 1928, is located in the Kherson region of Ukraine, near the railway station Tsiurupinsk. The Nizhnyodniprovsky Research Station "Afforestation of sands and viticulture" is located here. The total area ​​of forest fund in Kherson region is 221.7 thousand hectares. The percentage of forest land is 3.3 %. 45 thousand hectares of this area belong to Tiurupinsk forest. The tree composition involves 74 % of coniferous and 26 % of deciduous breeds. 62 % of Cherson forests were created by man. The pine forests on the Oleshkovsky (Nizhnyodniprovsky) sands were created in the middle of the XIX сentury. This was dictated by the urgent task of fixing the sands by the black storms, using the fertility of sands in forest managment and agriculture. Completely joining the authors of papers devoted Oleshkovsky sands, their afforestation, recreation, conservation and management of the southern pine forest complexes, we consider important to mention: in harsh growing conditions of pine trees it is extremely necessary to strive for a complex biogeocenological research, to a comprehensive in-depth knowledge of pine ecosystems, at which typological approach is obligatory not from the standpoint of common assessments of forest growth conditions, but using typological principles of Professor A. L. Belgard established for the conditions of geographical and often environmental inadequacy of forest to habitat conditions; the typology provides diversity of soil types of Oleshkovsky forest growth conditions where there are two variants of soils – with and without salinity, with different gradations of humidification – from very dry to wet soils; it is necessary to take into account the extent of the influence of planted forests on the environment, which depends primarily on the ecological forest structure, which refers to the light structure of the stands and the duration of their habitat transforming influence. Light structure, in its turn depends on the architectonics of the tree crowns forming part of the forest (Belgard, 1971); using the special equipment it is necessary to create or improve the network of hydrological monitoring wells covering all environmental profiles, catens and plots, to conduct large-scale monitoring studies of the cyclic and successional forms of dynamics of forest hydrology: groundwater level, the chemistry, radioecology, organic matter, biota and also flow direction of groundwater movement (hydraulically interconnected), their degree of contamination, sanitary toxicological and other features; to explore sandy soils for content and quality of humus to evaluate soil fertility (Orlov, 1981); to explore microclimatic regimes to identify critical data to the vitality of pine plantations; with all indicators of systematic characteristics of a pine (Pinus silvestris L.), it has about 100 species. In the culture of Ukraine there are about 35 species. But, as foresters observe, not every pine (Pinus silvestris L.). gives a good effect of growth and development in every kind of environmental ecotope. It is necessary to consider the differences between hereditary traits of burned 350 years old samples of the eternal pinewood in Samarsky forest and artificial pine plantations grown from seed material taken from a completely different habitat conditions. Oak acorns, collected in the floodplain of River Dniester and planted in the watershed of Gyrnetsovy forest in Moldavia, dieback at the age of 30 years, but oak acorns, collected in plakor conditions and landed next to the first, have high vitality, intensive growth and development. It is well known that the Scots pine (Pinus silvestris) and Cretaceous pine (Pinus cretacea) do not differ in systematics. But Scots pine planted on chalk mountains near Scots pine are different. The first pine does not give seed regeneration, and the second one has acquired the ability to reproduce itself easily on Cretaceous and to hоld on barren rock outcrops (Milkov, 1959); in the study of sandy habitats it is necessary to establish consort links in biogeocenoses, their horizontal and vertical structures, ecomorphic features of the forest, its age population type, and as a result - to establish the viability and sustainability of pine plantations to the conditions; finally, it should be emphasized that only a comprehensive and integrated approach to the study of forest ecosystems in the steppe (horizontal and vertical structure) can give a reliable information about the successfully constructed plantation, its stability and durability.


2017 ◽  
Vol 9 (3) ◽  
pp. 433-442
Author(s):  
Ali Asghar CHITBAND ◽  
Reza GHORBANI ◽  
Mohammad Hassan RASHED MOHASSEL ◽  
Mahbubeh NABIZADE

Chlorophyll fluorescence analysis is a simple and rapid method for detecting herbicide effects after a short time following their application in photosynthetic apparatus in plants. Chlorophyll fluorescence measurements were carried out against two broad of weeds to describe how the Kautsky curve and its parameters were affected by herbicides. Desmedipham + phenmedipham + ethofumesate changed the chlorophyll fluorescence induction curve at all time intervals except four hours after spring (HAS) in Amaranthus retroflexus L. and at all doses of Portulaca oleracea L. 4 HAS. In contrast, chlorophyll fluorescence inhibition was evident by chloridazon at doses of 650 and 325 g a.i. ha-1 in P. oleracea and A. retroflexus respectively, for all time intervals. Furthermore, chlorophyll fluorescence decays only occurred by clopyralid in A. retroflexus at the highest dose. A biomass effective dose (ED50 and/or ED90) based on log-logistic dose-response curves for A. retroflexus were considerably higher than that of P. oleracea. The maximum quantum efficiency (FV/Fm) was stable, whereas the relative changes at the J step (Fvj) and area (the area between the Kautsky curve and the maximum fluorescence (Fm)) was more sensitive to all three herbicides. There was a relatively good correlation between fluorescence parameters taken 24 hours after the spraying and the dry matter taken three weeks later, for both species under study.


Sign in / Sign up

Export Citation Format

Share Document