scholarly journals The effectiveness of the entomopathogenic fungus Metarhizium anisopliae in controlling the green leaf hopper (Nephotettix virescens)

2021 ◽  
Vol 911 (1) ◽  
pp. 012061
Author(s):  
Elisurya Ibrahim ◽  
Firmansyah Firmansyah ◽  
Sumarni Panikkai
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Research Station ◽  
Nephotettix Virescens ◽  
Green Leafhopper ◽  
Application Method ◽  
Death Time ◽  
Randomized Design

Abstract Green leafhopper (Nephotettix virescens) is one of the major pests on rice plants that can be a vector of tungro disease. Current pest control using insecticides by farmers is very worrying because in general it is not as recommended which can cause disturbances in the balance of the ecosystem, so it is necessary to use more environmentally friendly control alternatives, one of which is the use of entomopathogenic fungi that are targeted and do not cause the death of natural enemies. The potential of entomopathogenic fungi as biological control agents needs to be continuously developed to reduce the use of insecticides, one of which is the fungus Metarhizium anisopliae which is a type of entomopathogenic fungus that can kill insect pests. The purpose of this study was to determine the effect of several concentration and application method of M. anisopliae on the mortality of the green leafhopper (Nephotettix virescens). The study was conducted in the Laboratory and Greenhouse of the Tungro Disease Research Station starting from February - April 2020 using a two-factor factorial design in a completely randomized design (CRD). The first factor was the application method consisting of two treatments, namely the spray method (A1) and dip method (A2) while the second factor was the conidia density of Metarhizium anisopliae with 4 levels, namely 0 (C0) as control, conidia density 106 (C1) conidia density 107 (C2) conidia density 108(C3). Each treatment was repeated three times. The results showed that the concentration of 106 by dipping application showed the fastest average death time of green leafhopper imago, which occured after 4 days of application, while the fastest average death time of green leafhopper nymph was 3.67 days after application at a concentration of 10 7 by dipping applications.

scholarly journals Effects of the Entomopathogenic Fungus Metarhizium anisopliae on the Mortality and Immune Response of Locusta migratoria

Insects ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 36 ◽  
Author(s):  
Wuji Jiang ◽  
Yifan Peng ◽  
Jiayi Ye ◽  
Yiyi Wen ◽  
Gexin Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Locusta Migratoria ◽  
Management Strategies ◽  
Sod Activity ◽  
Immune Related Genes ◽  
Locusta Migratoria Manilensis

Entomopathogenic fungi are the key regulators of insect populations and some of them are important biological agents used in integrated pest management strategies. Compared with their ability to become resistant to insecticides, insect pests do not easily become resistant to the infection by entomopathogenic fungi. In this study, we evaluated the mortality and immune response of the serious crop pest Locusta migratoria manilensis after exposure to a new entomopathogenic fungus strain, Metarhizium anisopliae CQMa421. M. anisopliae CQMa421 could effectively infect and kill the L. migratoria adults and nymphs. The locust LT50 under 1 × 108 conidia/mL concentration of M. anisopliae was much lower than that under conidial concentration 1 × 105 conidia/mL (i.e., 6.0 vs. 11.2 and 5.0 vs. 13.8 for adults and nymphs, respectively). The LC50 (log10) of M. anisopliae against locust adults and nymphs after 10 days was 5.2 and 5.6, respectively. Although the number of hemocytes in L. migratoria after exposure to M. anisopliae did not differ with that in the controls, the enzymatic activity of superoxide dismutase (SOD) and prophenoloxidase (ProPO) did differ between the two treatments. The activities of both SOD and ProPO under the M. anisopliae treatment were lower than that in the controls, except for the ProPO activity at 72 h and the SOD activity at 96 h. Further, the expression of the L. migratoria immune-related genes defensin, spaetzle, and attacin differed after exposure to M. anisopliae for 24 h to 96 h. Taken together, this study indicated that infection with M. anisopliae CQMa421 could cause the death of L. migratoria by interacting with the immune responses of the host, demonstrating that this fungal strain of M. anisopliae can be an efficient biocontrol agent against L. migratoria.

scholarly journals Activity of strains of Metarhizium anisopliae (Metsch) Sorokin against white grub (Chiloloba acuta, Scarabaeidae, Coleoptera) under laboratory condition in Nepal

2018 ◽  
Vol 17 ◽  
pp. 82-91 ◽  
Author(s):  
Dipak Khanal
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
White Grub ◽  
White Grubs ◽  
Larval Stages ◽  
Commercial Strain ◽  
Randomized Design ◽  
Completely Randomized Design

Soil insect pests are the major productivity constrains of different crops among which white grubs (Coleoptera: Scarabaeidae), both adult and larval stages, are extremely destructive in nature. Laboratory studies were conducted to evaluate the virulence of an indigenous and a commercial strain of the entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sorokin, against white grubs species Chiloloba acuta by applying the dipping method at Entomology Division, NARC, Nepal. Third instars larvae of C. acuta were dipped in suspensions of indigenous and commercial strains (Pacer) of M. anisopliae at different concentrations ranging from 3.33×104 to 1.04×108 spores/ml for 3-5 seconds which resulted in 97.8% and 89% mortalities with the highest dose of 1.04×108 spores/ml 40 days after inoculation (DAI), respectively. The LC50 values for indigenous and commercial strain were 3.5×105 and 1.88×106 spores/ml, respectively, with the potency of 1:0.28 at 40 DAI. Bioassays were conducted in completely randomized design. From results it is concluded that the tested strains of entomopathogenic fungi have potential as microbial control agents in managing white grubs in laboratory and it is suggested to be tested under farmers field condition.

scholarly journals Patogenisitas Cendawan Metarhizium anisopliae (Metsch) dalam mengendalikan Kepik Hijau (Nezara viridula L.) pada Stadia Perkembangan yang Berbeda di Laboratorium

2020 ◽  
Vol 4 (2) ◽  
pp. 178-187
Author(s):  
Ahmad Alwi Azhari ◽  
Muhammad Sayuthi ◽  
Hasnah Hasnah
Keyword(s):  
Incubation Period ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Biological Control Agent ◽  
Insect Pest ◽  
Instar Nymph ◽  
Nezara Viridula ◽  
Stink Bug ◽  
Death Time

Abstrak. Nezara viridula (L.) (Hemiptera: Pentatomidae) adalah serangga hama yang bersifat kosmopolit dan mempunyai banyak tanaman inang (polifagus) antara lain kacang-kacangan dan kubis-kubisan. Serangan serangan hama ini dapat menurunkan kualitas dan kuantitas hasil tanaman. Salah satu metoda pengendalian hama yang ramah lingkungan yaitu penggunaan cendawan entomopatogen Metarhizium anisopliae sebagai agen pengendalian hayati yang dapat menyebabkan penyakit green muscardin fungus pada serangga. Keefektifan cendawan entomopatogen sangat dipengaruhi oleh stadia perkembangan inang dan kerapatan konidia cendawan yang diaplikasikan. Tujuan dari penelitian ini adalah untuk mendapatkan konsentrasi yang efektif dari cendawan M. anisopliae dan stadia perkembangan serangga yang paling rentan terhadap cendawan M. anisopliae. Rancangan yang digunakan dalam penelitian adalah Rancangan Acak Lengkap faktorial yang terdiri dari 2 faktor yaitu konsentrasi cendawan M. anisopliae: K1 (4g/100 ml), K2 (6g/100 ml) dan K3 (8g/100 ml) serta stadia perkembangan serangga: N1 (Nimfa instar 2), N2 (Nimfa instar 4) dan N3 (Imago). Menghasilkan jumlah kombinasi perlakuan 9 dengan 3 ulangan sehingga terdapat 27 unit percobaan. Hasil penelitian menunjukkan bahwa aplikasi cendawan entomopatogen M. anisopliae berpengaruh nyata terhadap masa inkubasi, mortalitas, dan waktu kematian dari N. viridula. Masa inkubasi tercepat pada 8g/ 100 ml yaitu 1,33 hari dan terlama pada 4g/ 100 ml yaitu 1,44 hari. Masa inkubasi tercepat pada nimfa instar 2 yaitu 1,00 hari dan terlama pada imago yaitu 2,11 hari. Mortalitas tertinggi yaitu pada 8g/ 100 ml sebesar 97,67% dan terendah pada 4g/ 100 ml sebesar 87,78% pengamatan 8 HSA. Mortalitas tertinggi terjadi pada nimfa instar 2 sebesar 98,89% dan terendah pada imago sebesar 86,67% pengamatan 8 HSA. Waktu kematian tercepat pada perlakuan 8g/ 100 ml sebesar 4,49 hari dan terendah pada perlakuan 4g/ 100 ml sebesar 5,50 hari. Waktu kematian tercepat pada nimfa instar 2 sebesar 4,31 hari dan terendah pada imago sebesar 6,00 hari. Cendawan entomopatogen M. anisopliae efektif dalam mengendalikan N. viridula pada nimfa instar 2 dengan konsentrasi 8g/ 100 ml.Pathogenicity of Fungi Metarhizium anisopliae (Metsch) in controlling Green Stink bug (Nezara viridula L.) at Different Developmental Stages in LaboratoryAbstract. Nezara viridula (L.) (Hemiptera: Pentatomidae) is a cosmopolitan insect pest and has many host plants (polyphagus) such as beans and cabbage. Attack of the pests can reduce the quality and quantity of crops. One of the most environmentally friendly methods of pest control is the use of entomopathogenic fungus Metarhizium anisopliae as a biological control agent can cause green muscard fungus disease in insects. The effectiveness of entomopathogenic fungi is strongly influenced by the stadia of host development and the density of conidial fungi applied. The aim of this study is to obtain an effective concentration of the fungus M. anisopliae and the stages of development of the most vulnerable insects against the fungus M. anisopliae. The design used in the study was a Factorial Randomized Complete Random consisting of two factors: the concentration of fungus M. anisopliae: K1 (4g / 100 ml), K2 (6g / 100 ml) and K3 (8g / 100 ml) and stages insect development: N1 (instar nymph 2), N2 (instar nymph 4) and N3 (Adult). Produced a combined number of treatments 9 with 3 replications so that there were 27 experimental units. The result showed that the application of entomopathogenic fungi M. anisopliae had significant effect on the incubation period, mortality, and death time of N. viridula. The fastest incubation period at 8g / 100ml is 1.33 days and the longest at 4g / 100ml is 1.44 days. The fastest incubation period in nymph instar 2 is 1.00 days and the longest in adult is 2.11 days. The highest mortality is at 8g / 100 ml of 97,67% and the lowest at 4g / 100 ml equal to 87,78% observation 8 HSA. The highest mortality occurred in instar nymph 2 of 98.89% and the lowest in adult of 86,67% observation 8 HSA. The fastest death time in the 8g / 100 ml treatment was 4.49 days and the lowest in the 4g / 100 ml treatment was 5.50 days. The fastest death time in instar nymph 2 is 4.31 days and the lowest is adult at 6.00 days. The entomopathogenic fungus M. anisopliae is effective in controlling N. viridula in instar nymph 2 with a concentration of 8g / 100 ml.

Different strategies to kill the host presented byMetarhizium anisopliaeandBeauveria bassiana

2018 ◽  
Vol 64 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Cynthia Barbosa Rustiguel ◽  
María Fernández-Bravo ◽  
Luis Henrique Souza Guimarães ◽  
Enrique Quesada-Moraga
Keyword(s):  
Secondary Metabolites ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Fungal Growth ◽  
Growth Strategy

Studies conducted over the last decades have shown the potential of entomopathogenic fungi for the biocontrol of some insect pests. Entomopathogenic fungi infect their host through the cuticle, so they do not need to be ingested to be effective. These fungi also secrete secondary metabolites and proteins that are toxic to insect pests. In this context, we analyzed the pathogenicity of Metarhizium anisopliae (Metschn.) strains IBCB 384 and IBCB 425 and Beauveria bassiana (Bals.-Criv.) Vuill. strains E 1764 and E 3158 against Galleria mellonella (Linn.) larvae, during pre-invasion and post-invasion phases. The results showed M. anisopliae, especially strain IBCB 384, was most virulent in the pre-invasion phase against G. mellonella, whereas B. bassiana, especially strain E 1764, was most virulent in the post-invasion phase. During in vivo development and in the production of toxic serum, B. bassiana E 3158 was the most virulent. Different fungal growth (or toxin) strategies were observed for studied strains. Metarhizium anisopliae IBCB 425 prioritizes the growth strategy, whereas strain IBCB 384 and B. bassiana strains E 1764 and E 3158 have a toxic strategy. All strains have pathogenicity against G. mellonella, indicating their possible use for biocontrol.

scholarly journals Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2365-2373 ◽  
Author(s):  
AYU SAFITRI ◽  
SITI HERLINDA ◽  
ARUM SETIAWAN
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Vegetation Type ◽  
Tenebrio Molitor ◽  
Soil Samples ◽  
Fungal Isolates

Safitri A, Herlinda S, Setiawan A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365-2373. Ecosystems of lowlands and highlands in South Sumatra have specific characteristics of soils and vegetations that can affect the availability of entomopathogenic fungi. This study aimed to explore and identify species and to determine inoculum potentials of the entomopathogenic fungi from soils of freshwater swamps, tidal lowlands, peatlands, and highlands. Baiting of entomopathogenic fungi on soil samples used the larvae of Tenebrio molitor. The entomopathogenic fungi species found in this research were Beauveria bassiana and Metarhizium anisopliae. The number of the fungal isolates were 30 isolates consisting of nine isolates of B. bassiana and 21 isolates of M. anisopliae.The highest number of isolates was found in the highland ecosystem (11 isolates) and the lowest was found in peatland ecosystem (4 isolates). The highest percentage average of inoculum potentials of the fungi was found in the high land ecosystem (4.04%) and the lowest one was found in freshwater swamps ecosystem (2.11%). Based on the vegetation type, the soil planted with mustard in Talang Patai-Pagaralam (highland ecosystem) had the highest inoculum potentials (9.33%). These fungi will make an important contribution to the biological control for insect pests in lowland to highland ecosystems in Indonesia.

scholarly journals Aplikasi IJEN (Infeksi Jamur Entomopatogen pada Nyamuk) : Jamur Metarhizium anisopliae pada Nyamuk Aedes aegypti

2019 ◽  
Vol 13 (1) ◽  
pp. 33-48
Author(s):  
Liestiana Indriyati ◽  
Salamiah Salamiah ◽  
Lutfhi Fatah ◽  
Eko Suhartono ◽  
Muhammad Rasyid Ridha ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Olive Oil ◽  
Experimental Research ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Growth ◽  
Hemorrhagic Fever ◽  
Small Risk ◽  
Randomized Design ◽  
Eggs And Larvae

Abstract Dengue hemorrhagic fever is one of the main problems in Indonesia, its handling on chemical insecticides with insecticide resistance constraints that can inhibit vector control efforts. Entomopathogenic fungi especially Metarhizium anisopliae is one of bioinsecticides that has been widely used in agriculture for controlling insect attacks, effective in killing mosquitoes, safe for vertebrates, humans and the environment and has a small risk of resistance. Appropriate methods of formulation and application for M. anisopliae infection to mosquitoes suitable for mosquito bionomic and fungus characteristics are required. Experimental research with complete randomized design was conducted to test the efficacy of.  M.anisopliae solution mixed olive oil and honey formula applied to ovitrap, plant trap and black cotton trap in infecting adult female Ae. aegypti. The death of Ae.a egypti and fungal growth on kadaver was observed everyday. 100% Ae. aegypti death was obtained in the ovitrap method with mixed formulation of M.anisopliae and olive oil. This method was also capable of infecting Ae.aegypti in various phases of both adults, eggs and larvae so that in this study ovitrap olive oil method was the most effective method for infecting M.anisopliae on Ae.aegypti. Abstrak Demam berdarah dengue merupakan salah satu masalah utama di Indoneisa. Penggunaan insektisida kimia sebagai upaya pengendalian DBD saat ini memiliki kendala resistensi insektisida yang dapat menghambat upaya pengendalian vektor. Jamur entomopatogen khususnya Metarhizium anisopliae adalah salah satu bioinsektisida yang telah digunakan secara luas di bidang pertanian untuk pengendalian serangan serangga efektif membunuh nyamuk, aman bagi vertebrata, manusia dan lingkungan serta memiliki risiko resistensi yang kecil. Diperlukan metode yang tepat baik dari formulasi maupun cara aplikasi untuk penginfeksian M.anisopliae kepada nyamuk yang sesuai dengan bionomik nyamuk dan karakteristik jamur. Penelitian eksperimental dengan rancangan acak lengkap dilakukan untuk menguji efektivitas formula M.anisopliae. Larutan  M.anisopliae dicampur minyak zaitun dan madu yang diaplikasikan pada ovitrap, plant trap dan black cotton trap dalam menginfeksi Ae.aegypti betina dewasa. Pengamatan dilakukan pada  kematian Ae.aegypti dan pertumbuhan jamur pada kadaver setiap hari. Kematian Ae.aegypti 100% didapatkan pada metode ovitrap dengan formulasi campuran M.anisopliae dan minyak zaitun. Metode ini juga mampu menginfeksi Ae.aegypti pada berbagai fase baik dewasa, telur maupun larva sehingga pada penelitian ini metode zaitun ovitrap dinyatakan metode yang paling efektif untuk penginfeksian M.anisopliae pada Ae.aegypti.

scholarly journals KOMPATIBILITAS CENDAWAN METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN DENGAN NEMATODA ENTOMOPATOGEN STEINERNEMA SP.

2017 ◽  
Vol 17 (2) ◽  
pp. 111
Author(s):  
Imron Rosyidi ◽  
Hari Purnomo ◽  
Nanang Tri Haryadi ◽  
Mohammad Hoesain
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Research Result ◽  
Symbiotic Bacteria ◽  
Randomized Design ◽  
Completely Randomized Design

Compatibility Metarhizium anisopliae (Metschn.) Sorokin with entomopathogenic nematode Steinernema sp. Metarhizium anisopliae (Metschn.) Sorokin and entomopathogenic nematode Steinernema sp. can be used as biological control of insect pests. The objective of this research was to identify compatibility between fungus M. anisopliae with entomopathogenic nematode Steinernema sp. This research was conducted using a completely randomized design (CRD) consisting of 5 treatments and repeated 5 times. The treatment used was M. anisopliae isolates Jombang 1 with Steinernema sp. (M1), M. anisopliae Jombang 2 with Steinernema sp. (M2), M. anisopliae isolates Kediri with Steinernema sp. (M3), M. anisopliae isolates Bondowoso with Steinernema sp. (M4), and M. anisopliae isolates Banyuwangi with Steinernema sp. (M5). Based on this research result, compatibility between the fungus M. anisopliae with entomopathogenic nematode Steinernema sp. was antagonistic. This occurs due to the symbiotic bacteria Xenorhabdus sp. that produced compounds antimycotic and capable to inhibit the growth of fungus M. anisopliae.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

scholarly journals COMPATIBILITY OF NEUTRAL DETERGENT WITH THE ENTOMOPATHOGENIC FUNGUS METARHIZIUM ANISOPLIAE (METSCH.) SOROK.

2011 ◽  
Vol 78 (3) ◽  
pp. 471-474
Author(s):  
E.S. Harterreiten-Souza ◽  
L.G.A. Pessoa ◽  
E. de S. Loureiro
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Vegetative Growth ◽  
Entomopathogenic Fungus ◽  
Tween 80 ◽  
Genetic Composition ◽  
Fungus Species ◽  
Hydrophobic Character ◽  
Colony Forming Units ◽  
Conidia Production

ABSTRACT Entomopathogenic fungi have been widely used in pests control. The main diluent for its application in the field is water, and due to its hydrophobic character, its dispersion in the environment is complicated. Several products may be mixed to the sprays, allowing the dissolution and the dispersion of the fungus in the spray. Yet, some of these products may influence in the viability, sporulation or even in its genetic composition, changing its virulence. This paper aimed to evaluate the effect of a neutral detergent about the viability of the entomopathogenic fungus Metarhizium anisopliae. Three different concentrations of a neutral detergent (Ipê®) were used: 0.01%, 0.02%, 0.03% and an adhesive spreader (Tween 80) was used in the control. The percentage of conidia germination, the number of colony forming units (CFU), vegetative growth and the number of produced conidia were evaluated. There was no effect of the different concentrations of the neutral detergent on the germination. All detergent concentrations affected negatively in the vegetative growth of the fungus. Only the detergent at 0.01% of concentration did not affect the CFU number and the conidia production of the fungus. So, this is the only concentration classified as compatible for M. anisopliae, which may be recommended to promote the conidia dispersion of this fungus species in water.

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