Domestication Strategies of Tinfoil Barb Barbonymus schwanenfeldii (Bleeker, 1854): Potential Candidate for Freshwater Aquaculture Development

Abstract This paper discussed domestication strategies of Tinfoil barb from different populations for evaluation of potential fish candidate for freshwater aquaculture development. Collection and maintenance of wild-caught adult fish was the first step in domestication strategies followed by characterization of fish, broodstocks selection and maturation, control of fish reproduction, and larval rearing. The Tinfoil barb presented an excellent performance to adapt to the new environment. This was indicated by survival rate of the fish acclimated in the captive environment of about 69.28 ± 19.64% for five weeks. Phenotypic characterization revealed that Tinfoil barb collected from Borneo, Java, and Sumatra have different morphological characters. Based on genetic characters, polymorphism percentage and heterozygosity of the Borneo tinfoil barb population provided the highest performance followed by Java and Sumatra. The growth model of all Tinfoil barb populations was allometric negative indicating a faster increase in length than in weight. Reproductive performance presented that the fecundity of Tinfoil barb female from Borneo was the highest (2795-5099 eggs/gram) followed by Sumatra (1822-2976 eggs/gram) and Java (2210 eggs/gram). The artificial reproduction performance of broodstock from Borneo also presented more excellent performance than other population after hormonal treatment. Fertility rate and hatching rate were 94,33+0,29 % and 77,44+1,03%, respectively. Java Tinfoil barb had fertility rate 93+0,05% and hatching rate 73,67+3,15%, while it was no fertility rate and hatching rate obtained from Sumatra brood-stock group. Wild-caught adult Tinfoil barb from Borneo population presented the best performance used for potential brood-stock regarding genetic characters, reproductive performances, and response to artificial reproduction. Future studies need to be concerned with the acceleration of gonad maturation and the improvement of larval rearing technology for improvement of domestication strategies of Tinfoil barb as a potential candidate for freshwater aquaculture development.

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REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra)

Marine Research in Indonesia ◽

10.14203/mri.v43i1.267 ◽

2018 ◽

Vol 43 (1) ◽

pp. 11-17

Author(s):

Sari Budi Moria Sembiring ◽

Gigih Setia Wibawa ◽

I Nyoman Adiasmara Giri ◽

Jhon Harianto Hutapea ◽

Haryanti

Keyword(s):

Body Weight ◽

Growth Performance ◽

Sea Cucumber ◽

Survival Rates ◽

Hatching Rate ◽

Larval Rearing ◽

Holothuria Scabra ◽

Total Length ◽

And Performance ◽

Aquaculture Development

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.

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REPRODUKSI IKAN BAUNG (Hemibagrus nemurus) DENGAN PERLAKUAN DOSIS HORMON GnRH-a BERBEDA

Jurnal Riset Akuakultur ◽

10.15578/jra.13.3.2018.213-218 ◽

2018 ◽

Vol 13 (3) ◽

pp. 213

Author(s):

Jojo Subagja ◽

Vitas Atmadi Prakoso

Keyword(s):

Body Weight ◽

Survival Rate ◽

Larval Survival ◽

Fertilization Rate ◽

Fertility Rate ◽

Time Interval ◽

Hatching Rate ◽

Good Prospect ◽

Hormonal Manipulation ◽

Different Doses

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.

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Infection of highly insecticide-resistant malaria vector Anopheles coluzzii with entomopathogenic bacteria Chromobacterium violaceum reduces its survival, blood feeding propensity and fecundity

10.21203/rs.2.23058/v4 ◽

2020 ◽

Author(s):

Edounou Jacques Gnambani ◽

Etienne Bilgo ◽

Adama Sanou ◽

Roch K. Dabire ◽

Abdoulaye Diabate

Keyword(s):

Burkina Faso ◽

Post Infection ◽

Blood Feeding ◽

Egg Laying ◽

Chromobacterium Violaceum ◽

Hatching Rate ◽

Anopheles Coluzzii ◽

Potential Candidate ◽

Mosquito Survival ◽

The Impact

Abstract Background This is now a concern that malaria eradication will not be achieved without the introduction of novel control tools. Microbiological control might be able to make a greater contribution to vector control in the future. The interactions between bacteria and mosquito make mosquito microbiota really promising from a disease control perspective. Here, we studied the impact of Chromobacterium violaceum infections isolated from both larvae and adult of wild caught Anopheles gambiae s.l. mosquitoes in Burkina Faso on mosquito survival, blood feeding and fecundity propensy. Methods To assess entomopathogenic effects of C. violaceum infection on mosquitoes, three different types of bioassays were performed in laboratory. These bioassays aimed to evaluate the impact of C.violaceum infection on mosquito survival, blood feeding and fecundity, respectively. During bioassays mosquitoes were infected through the well-established system of cotton ball soaked with 6% glucose containing C.violaceum . Results C. violaceum kills pyrethroid resistant mosquitoes An. coluzzii (LT80 of 8.78 days ± 0.18 at 10 8 bacteria cell/ml of sugar meal). Interestingly, this bacterium had other negative effects on mosquito lifespan by significantly reducing (~59%, P<0.001) the mosquito feeding willingness from day 4-post infection (~81% would seek a host to blood feed) to 9- day post infection (22 ± 4.62% would seek a host to blood feed). Moreover, C. violaceum considerably jeopardized the egg laying (~16 eggs laid /mosquitoes with C. violaceum infected mosquitoes vs ~129 eggs laid / mosquitoes with control mosquitoes) and hatching of mosquitoes (A reduction of ~22 % of hatching rate with C. violaceum infected mosquitoes). Compared to the bacterial uninfected mosquitoes, mosquitoes infected with C. violaceum showed indeed significantly higher retention rates of immature eggs and follicles. Conclusion These data showed important properties of Burkina Faso C. violaceum strains , which are highly virulent against insecticide resistant Anopheles coluzzii , and reduce both mosquito blood feeding and fecundity propensities. However, additional studies as the sequencing of C. violaceum genome and the potential toxins secreted will certainly provide useful information render it a potential candidate for the biological control strategies of malaria and other disease vectors.

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ASPEK MORFOLOGI, REPRODUKSI, DAN PERILAKU PENYU HIJAU (Chelonia mydas) Di PANTAI PANGUMBAHAN, KABUPATEN SUKABUMI, JAWA BARAT

BAWAL Widya Riset Perikanan Tangkap ◽

10.15578/bawal.3.2.2010.93-101 ◽

2017 ◽

Vol 3 (2) ◽

pp. 93

Author(s):

Adriani Sri Nastiti Krismono ◽

Achmad Fitriyanto ◽

Ngurah Nyoman Wiadnyana

Keyword(s):

Green Turtle ◽

Carapace Width ◽

Chelonia Mydas ◽

Sea Turtle ◽

Stratified Sampling ◽

Hatching Rate ◽

Brood Stock ◽

Green Turtles ◽

And Behavior ◽

Nesting Season

Penyu hijau (Chelonia mydas) termasuk dalam phylum Chordata dan famili Cheloniideae. Jumlah penyu hijau yang singgah ke Pantai Pangumbahan untuk bertelur semakin menurun karena tidak terkendalinya masyarakat melakukan penangkapan induk penyu dan pengambilan telurnya. Penelitian tentang morfologi, reproduksi, dan perilaku penyu hijau sebagai salah satu dasar pengelolaan telah dilakukan di Pantai Pangumbahan pada bulan Agustus 2008. Metode penelitian yang digunakan pengambilan contoh berstrata. Penelitian ini dilaksanakan pada bulan Agustus 2008 (pada saat puncak peneluran). Parameter yang diamati antara lain ukuran penyu dari 89 ekor jumlah penyu bersarang, jumlah telur dan tingkat penetasan, serta perilaku pada saat penyu bertelur. Hasil penelitian menunjukan yang diamati diperoleh panjang karapas berkisar antara 97-15 cm dan lebar karapas 83,5-108 cm, jumlah penyu naik ke pantai 89 ekor dan penyu yang bertelur 39 ekor. Jumlah telur penyu hijau berhasil dihitung 80-105 butir per induk penyu. Bulan Agustus 2008 merupakan puncak musim peneluran. Kegiatan peneluran penyu hijau dibagi menjadi enam tahap. Upaya konservasi yang sudah dilakukan adalah penetasan telur penyu semi alami, restocking tukik, dan menjaga keamanan sarang telur penyu. Green turtles (Chelonia mydas), including the phylum Chordata and families Cheloniideae. The number of green turtles come to lay eggs Pangumbahan beach to decline because of increasingly unmanageable public do making arrests turtles brood stock and their eggs. Research on the morphology, reproduction, and behavior of green turtles as one of the basic management has been conducted on the Pangumbahan Beach in August 2008. The method used stratified sampling. The experiment was conducted in August 2008 (at the peak of nesting). Other parameters were observed between the size of the 89 tail number of turtles nesting turtles, the number of eggs and hatching rate and behavior during turtle nesting. Results obtained showed that the observed length ranges from 97- 15 cm carapace and carapace width from 83.5-108 cm, the number went up to the beach 89 sea turtle and sea turtle nesting tail as much as 39 tails. The number of green turtle eggs had counted as many as 80-105 eggs per turtles brood stock. Month August 2008 is the peak nesting season of green turtle nesting activities are divided into six stages. Conservation efforts that have been done is semi natural turtle hatchery, restocking hatchlings, and nest of turtle eggs to maintain security.

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Utilization Efficiency of Yolk Egg on Maanvis (Pterophyllum scalare) Embryos and Larvae in Different Incubation Temperatures

Jurnal Akuakultur Indonesia ◽

10.19027/jai.4.57-61 ◽

2007 ◽

Vol 4 (1) ◽

pp. 57 ◽

Cited By ~ 1

Author(s):

T. Budiardi ◽

W. Cahyaningrum ◽

I. Effendi

Keyword(s):

Growth Rate ◽

Incubation Time ◽

Egg Yolk ◽

Utilization Efficiency ◽

Hatching Rate ◽

Larval Rearing ◽

Optimum Temperature ◽

Egg Hatching ◽

Incubation Temperatures ◽

Pterophyllum Scalare

This study was performed to determine the efficiency of yolk egg utilization in embryos and larvae, hatching rate, incubation time to hatch, and growth rate of maanvis (Pterophyllum scalare) larvae incubated at room remperature, 27oC, and 30oC. Results of study showed that yolk egg utilization efficiency of embryos and larvae incubated at 30oC was 73.70% and 0,18%, respectively, and no different with that of room and 27oC incubation temperatures. Hatching rate of eggs incubated at 30oC (84.75%) was also same with that of other treatments. However, incubation time to hatch (27.41 hours) was shorter than that of other treatments. The growth rate by length of larvae (2.16%) and survival rate (75.28%) incubated at 30oC was also higher compared with that of other treatments. Thus, in general, optimum temperature for egg hatching and larval rearing of maanvis was 30oC. Keywords: maanvis, Pterophyllum scalare, egg yolk, larvae, embryo, temperature ABSTRAK Penelitian ini dilakukan untuk mengetahui efisiensi pemanfaatan kuning telur pada embrio dan larva, derajat penetasan, lama inkubasi telur hingga menetas, dan laju pertumbuhan serta kelangsungan hidup larva ikan maanvis (Pterophyllum scalare) yang diinkubasi pada suhu ruang, 27oC dan 30oC. Hasil penelitian menunjukkan bahwa nilai efisiensi pemanfaatan kuning telur bila diinkubasi pada suhu 30oCsebesar 73,70% pada fase embrio dan 0,18% pada fase larva, dan tidak berbeda dengan suhu ruang dan 27oC. Demikian juga dengan derajat penetasan telur (84,75%) tidak berbeda dengan perlakuan lainnya. Sementara itu, lama inkubasi telur hingga menetas (27,41 jam) lebih cepat dibandingkan dengan suhu inkubasi perlakuan lainnya. Demikian juga dengan laju pertumbuhan panjang (2,16%) dan kelangsungan hidup larva (75,28%) lebih tinggi dibandingkan dengan perlakuan lainnya. Dengan demikian, secara umum suhu optimal untuk penetasan dan pemeliharaan larva ikan maanvis adalah 30°C. Kata kunci: ikan maanvis, Pterophyllum scalare, kuning telur, larva, embrio, suhu

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Infection of highly insecticide-resistant malaria vector Anopheles coluzzii with entomopathogenic bacteria Chromobacterium violaceum reduces its survival, blood feeding propensity and fecundity

10.21203/rs.2.23058/v3 ◽

2020 ◽

Author(s):

Edounou Jacques Gnambani ◽

Etienne Bilgo ◽

Adama Sanou ◽

Roch K. Dabire ◽

Abdoulaye Diabate

Keyword(s):

Burkina Faso ◽

Post Infection ◽

Blood Feeding ◽

Egg Laying ◽

Chromobacterium Violaceum ◽

Hatching Rate ◽

Anopheles Coluzzii ◽

Potential Candidate ◽

Mosquito Survival ◽

The Impact

Abstract Background This is now a concern that malaria eradication will not be achieved without the introduction of novel control tools. Microbiological control might be able to make a greater contribution to vector control in the future. The interactions between bacteria and mosquito make mosquito microbiota really promising from a disease control perspective. Here, we studied the impact of Chromobacterium violaceum infections isolated from both larvae and adult of wild caught Anopheles gambiae s.l. mosquitoes in Burkina Faso on mosquito survival, blood feeding and fecundity propensy. Methods To assess entomopathogenic effects of C. violaceum infection on mosquitoes, three different types of bioassays were performed in laboratory. These bioassays aimed to evaluate the impact of C.violaceum infection on mosquito survival, blood feeding and fecundity, respectively. During bioassays mosquitoes were infected through the well-established system of cotton ball soaked with 6% glucose containing C.violaceum.Results C. violaceum kills pyrethroid resistant mosquitoes An. coluzzii (LT80 of 8.78 days ± 0.18 at 108 bacteria cell/ml of sugar meal). Interestingly, this bacterium had other negative effects on mosquito lifespan by significantly reducing (~59%, P<0.001) the mosquito feeding willingness from day 4-post infection (~81% would seek a host to blood feed) to 9- day post infection (22 ± 4.62% would seek a host to blood feed). Moreover, C. violaceum considerably jeopardized the egg laying (~16 eggs laid /mosquitoes with C. violaceum infected mosquitoes vs ~129 eggs laid / mosquitoes with control mosquitoes) and hatching of mosquitoes (A reduction of ~22 % of hatching rate with C. violaceum infected mosquitoes). Compared to the bacterial uninfected mosquitoes, mosquitoes infected with C. violaceum showed indeed significantly higher retention rates of immature eggs and follicles. Conclusion These data showed important properties of Burkina Faso C. violaceum strains, which are highly virulent against insecticide resistant Anopheles coluzzii, and reduce both mosquito blood feeding and fecundity propensities. However, additional studies as the sequencing of C. violaceum genome and the potential toxins secreted will certainly provide useful information render it a potential candidate for the biological control strategies of malaria and other disease vectors.

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FLUORESCENT LAMP LIGHT INTENSITY ON THE EMBRYOGENESIS DEVELOPMENT AND THE SURVIVAL OF PEARL OYSTER (Pinctada maxima) LARVAE

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v5i2.7567 ◽

2013 ◽

Vol 5 (2) ◽

Author(s):

Mat Sardi Hamzah

Keyword(s):

Survival Rate ◽

Light Intensity ◽

Pearl Oyster ◽

Larval Rearing ◽

Fluorescent Lamp ◽

Brood Stock ◽

Dark Treatment ◽

Light Intensities ◽

Significant Difference ◽

Pinctada Maxima

One of the important factors in determining the success of pearl oyster (Pinctada maxima) culture is the quality and quantity of larvae produced in brood stock spawning process in the laboratory. Problems were often found in larval rearing and larvae attachment to the substrate that were low in quality and little number of larvae. The study purposes were observe the embryogenesis development and the survival rate of pearl oyster larvae under different fluorescent lamp light intensities. The study was conducted in August 1nd – 30th, 2011 in Sambelia Bumi Gemilang Hamparan Mutiara laboratory, East Lombok. Results revealed that different in light intensities effected the survivorship of the pearl oyster larvae significantly (p<0.01). Honest Significant Difference (HSD) test indicated that the highest survival rate occurred in dark condition (dark treatment) of 38%, followed by intensity of 10 watts (34.67%), 5 watts (30.67%) and 15 watts (4.66%) resfectively Keywords: embryogenesis development, survivorship, pearl oyster (Pinctada maxima) larvae, fluorescent lamp light intensity

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FLUORESCENT LAMP LIGHT INTENSITY ON THE EMBRYOGENESIS DEVELOPMENT AND THE SURVIVAL OF PEARL OYSTER (Pinctada maxima) LARVAE

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.28930/jitkt.v5i2.7567 ◽

2013 ◽

Vol 5 (2) ◽

Author(s):

Mat Sardi Hamzah

Keyword(s):

Survival Rate ◽

Light Intensity ◽

Pearl Oyster ◽

Larval Rearing ◽

Fluorescent Lamp ◽

Brood Stock ◽

Dark Treatment ◽

Light Intensities ◽

Significant Difference ◽

Pinctada Maxima

One of the important factors in determining the success of pearl oyster (Pinctada maxima) culture is the quality and quantity of larvae produced in brood stock spawning process in the laboratory. Problems were often found in larval rearing and larvae attachment to the substrate that were low in quality and little number of larvae. The study purposes were observe the embryogenesis development and the survival rate of pearl oyster larvae under different fluorescent lamp light intensities. The study was conducted in August 1nd – 30th, 2011 in Sambelia Bumi Gemilang Hamparan Mutiara laboratory, East Lombok. Results revealed that different in light intensities effected the survivorship of the pearl oyster larvae significantly (p<0.01). Honest Significant Difference (HSD) test indicated that the highest survival rate occurred in dark condition (dark treatment) of 38%, followed by intensity of 10 watts (34.67%), 5 watts (30.67%) and 15 watts (4.66%) resfectively Keywords: embryogenesis development, survivorship, pearl oyster (Pinctada maxima) larvae, fluorescent lamp light intensity

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Kelangsungan Hidup Larva Ikan Nila yang Berasal Dari Induk yang Diberi Pakan Berimunostimulan

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.2.3.2014.5699 ◽

2014 ◽

Vol 2 (3) ◽

Author(s):

Ayu Maleko ◽

Hengky J Sinjal ◽

Henky Manoppo

Keyword(s):

Survival Rate ◽

Nile Tilapia ◽

Research Result ◽

Baker’S Yeast ◽

Larval Rearing ◽

Baker's Yeast ◽

Brood Stock ◽

Larval Quality ◽

Different Doses ◽

Increase Survival Rate

The objective of this research was to evaluate the effect of feed supplemented with baker’s yeast on survival rate of nile tilapia larvae and to determine the effective dose of baker’s yeast that could optimally improve larval quality. The research was conducted at Pathology and Clinic of Fish Disease from June – September 2014. Fish as brood stock candidates was cultivated at five concrete tanks (2x1x1 m3) at a density of 30 fish per tank. Each tank was equipped with water inlet, outlet and water pump for recirculating and aerating. After seven days of adaptation, fish was fed pellet supplemented with baker’s yeast at five different doses (0, 10, 20, 30, 40 g/kg food) for four consecutive weeks at 4%/bb/day, twice daily. Larva was captured with seser and moved into five glass aquaria with density of 20 larvae/aquarium and reared for two weeks. Data collected was survival rate of larvae achieved until the end of larval rearing. Research result showed that larvae produced by brood stock fed food supplemented with 10 g baker’s yeast per kg food had the highest survival rate namely 60%. As conclusion, supplementation of baker’s yeast into brood stock feed might increase survival rate of larvae. Keywords: nile tilapia, baker’s yeast, larvae, survival rate

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EXPERIMENT ON ARTIFICIAL REPRODUCTION OF BROCCOLI (Trichogaster Lalius) WITH HCG AT DIFFERENT DOSAGES

Scientific Journal of Tra Vinh University ◽

10.35382/18594816.1.33.2019.143 ◽

2019 ◽

Vol 1 (33) ◽

pp. 68-76

Author(s):

Son Phuoc Lai ◽

Tu Thi Cam Le

Keyword(s):

Survival Rate ◽

Environmental Factors ◽

Fertilization Rate ◽

Reproductive Efficiency ◽

Hatching Rate ◽

Maturation Process ◽

Artificial Reproduction ◽

Conditioning Process ◽

Maturation Rate ◽

Different Doses

The trail on artificial reproduction of dwarf gourami(Trichogaster lalius) with HCG at different dosages is to identify the maturation ability of Gophers (Trichogaster lalius) and compare the influence of HCG at different doses on the reproductive efficiency of dwarf gourami.The experiment consisted of 4 treatments (NT): 3000UI(NT1), 4000UI(NT2), 5000UI(NT3), 6000UI/kg(NT4) females. Each treatment was repeated 3 times. The results showed that during the conditioning process, environmental factors such as temperature and pH were in the appropriate range for the maturation process of broodstock. The highest maturation rate is 40% in females and 45.83% in males. The survival rate in the conditioning process was quite low at 88.33% in males and 66.67% in females, which however did not affect the results of the experiment. For stimulation to reproduction by HCG at different doses, the fertility rate fluctuates from 33.33% - 66.67% fertility ability ranges from 268.56 to 389.72 eggs / g female, fertilization rate 61.50%, and hatching rate 98.71%. The most appropriate dose of HCG for broccoli is 4000UI and the highest fertility of the four treatments is 389.72 eggs/g females.

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