scholarly journals The Discovery of a Potential Antimicrobial Agent: the Novel Compound Natural Medicinal Plant Fermentation Extracts against Candida albicans

2018 ◽  
Vol 301 ◽  
pp. 012026 ◽  
Author(s):  
Mingzhu Song ◽  
Xirui Wang ◽  
Canquan Mao ◽  
Wei Yao
Keyword(s):  
Candida Albicans ◽  
Medicinal Plant ◽  
Antimicrobial Agent ◽  
The Novel

scholarly journals The Novel Arylamidine T-2307 MaintainsIn VitroandIn VivoActivity against Echinocandin-Resistant Candida albicans

2014 ◽  
Vol 59 (2) ◽  
pp. 1341-1343 ◽  
Author(s):  
Nathan P. Wiederhold ◽  
Laura K. Najvar ◽  
Annette W. Fothergill ◽  
Rosie Bocanegra ◽  
Marcos Olivo ◽  
...  
Keyword(s):  
Body Weight ◽  
Candida Albicans ◽  
Invasive Candidiasis ◽  
Placebo Control ◽  
The Novel ◽  
Content Type ◽  
Fungal Burden ◽  
Potential Use

ABSTRACTWe evaluated thein vitroandin vivoactivities of the investigational arylamidine T-2307 against echinocandin-resistantCandida albicans. T-2307 demonstrated potentin vitroactivity, and daily subcutaneous doses between 0.75 and 6 mg/kg of body weight significantly improved survival and reduced fungal burden compared to placebo control and caspofungin (10 mg/kg/day) in mice with invasive candidiasis caused by an echinocandin-resistant strain. Thus, T-2307 may have potential use in the treatment of echinocandin-resistantC. albicansinfections.

scholarly journals Sensitive Assay for Antifungal Activity of Glucan Synthase Inhibitors That Uses Germ Tube Formation in Candida albicans as an End Point

2003 ◽  
Vol 47 (10) ◽  
pp. 3305-3310 ◽  
Author(s):  
Timothy G. Brayman ◽  
John W. Wilks
Keyword(s):  
Candida Albicans ◽  
Drug Discovery ◽  
Antifungal Activity ◽  
Germ Tube ◽  
Early Stage ◽  
Tube Formation ◽  
The Novel ◽  
Glucan Synthase ◽  
Drug Concentrations ◽  
Coefficients Of Variation

ABSTRACT We implemented a simple, sensitive, objective, and rapid cellular assay to reveal the antifungal activity of a novel class of glucan synthase inhibitors. The assay, especially useful for early drug discovery, measures the transformation of Candida albicans from the yeast form to the hyphal form. Test compounds were ranked by potency (50% inhibitory concentration) and efficacy (percent inhibition of germ tube formation); the intra-assay coefficients of variation for these parameters were 17 and 5%, respectively. The germ tube formation assay proved useful for the early-stage antifungal characterization of a novel class of glucan synthase inhibitors discovered at Pharmacia. Drug concentrations required in this assay to inhibit germ tube formation were lower for 90% of the novel compounds than the concentrations required to determine MICs. The method may have utility for other mechanistic classes of antifungal compounds during the hit-to-lead transition of drug discovery.

scholarly journals In VitroActivity against Staphylococcus aureus of a Novel Antimicrobial Agent, PRF-119, a Recombinant Chimeric Bacteriophage Endolysin

2011 ◽  
Vol 55 (9) ◽  
pp. 4416-4419 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Christof von Eiff ◽  
Alexander W. Friedrich ◽  
Domenico Iannelli ◽  
Guoqing Xia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agent ◽  
The Novel ◽  
Good Activity ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Content Type ◽  
Microdilution Method ◽  
Antistaphylococcal Activity ◽  
Bacteriophage Endolysin

ABSTRACTAntistaphylococcal activity of the novel chimeric endolysin PRF-119 was evaluated with the microdilution method. The MIC50and MIC90of 398 methicillin-susceptibleStaphylococcus aureusisolates were 0.098 μg/ml and 0.391 μg/ml, respectively (range, 0.024 to 0.780 μg/ml). Both the MIC50and MIC90values of 776 methicillin-resistantS. aureusisolates were 0.391 μg/ml (range, 0.024 to 1.563 μg/ml). All 192 clinical isolates of coagulase-negative staphylococci exhibited MIC values of >50 μg/ml. In conclusion, PRF-119 exhibited very good activity specifically againstS. aureus.

scholarly journals Candida albicans Antimicrobial and Antibiofilm Activity of Novel Endodontic Solvents

2021 ◽  
Vol 11 (16) ◽  
pp. 7748
Author(s):  
Inês Ferreira ◽  
Maria Elisa Rodrigues ◽  
Liliana Fernandes ◽  
Mariana Henriques ◽  
Irene Pina-Vaz
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Treatment ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Solvent Mixtures ◽  
The Novel ◽  
Disk Diffusion Method ◽  
Colony Forming Units ◽  
Endodontic Infections ◽  
Additional Exposure

Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures’ exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol.

scholarly journals PERBANDINGAN KADAR FENOLIK DAN AKTIVITAS ANTIMIKROBA RIMPANG JERINGAU (Acorus calamus) SEGAR DAN TERFERMENTASI

2020 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Fitri Hardiansi ◽  
Dwi Afriliana ◽  
Anita Munteira ◽  
Ernanin Dyah Wijayanti
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Antimicrobial Agent ◽  
Phenolic Content ◽  
Plant Cells ◽  
Acorus Calamus ◽  
Antimicrobial Test

ABSTRACTCalamus rhizome contain phenolic as antimicrobial agent. Fermentation can increase thereleasing of phenolic content from plant cells. The aim of this research was to observe thecomparison of phenolic content and antimicrobial activity between fresh and fermentedcalamus rhizome extract. Phenolic content was determined by using Folin-Ciocalteu method,and antimicrobial test by agar well diffusion. Phenolic content of fresh and fermentedcalamus rhizome extract respectively are 97,272±0,525 and 223,553±3,542 mgGAE/gram.Inhibiton zone against Staphylococcus aureus are 4,04±0,05 and 8,24±0,58 mm, whileagainst Candida albicans are 14,90±0,57 and 18,16±1,47 mm. Fermentation increase phenoliccontent and antimicrobial activity of calamus rhizome.Key words: antimicrobial, calamus rhozome, fermentation, phenolicABSTRAKRimpang jeringau memiliki kandungan senyawa fenolik sebagai antimikroba. Fermentasidapat meningkatkan pelepasan senyawa fenolik pada sel tanaman. Penelitian ini bertujuanuntuk mengetahui perbandingan kadar fenolik dan aktivitas antimikoba ekstrak rimpangjeringau segar dan terfermentasi. Penetapan kadar fenolik dilakukan dengan metode Folin-Ciocalteu dan uji antimikroba dengan metode difusi sumuran. Kadar fenolik ekstrak jeringausegar dan fermentasi secara berurutan sebesar 97,272±0,525 dan 223,553±3,542mgGAE/gram. Daya hambat rimpang jeringau segar dan terfermentasi terhadapStaphylococcus aureus sebesar 4,04±0,05 dan 8,24±0,58 mm, sedangkan terhadap Candidaalbicans sebesar 14,90±0,57 dan 18,16±1,47 mm. Fermentasi dapat meningkatkan kadarfenolik dan aktivitas antimikroba rimpang jeringau.Kata kunci: antimikroba, fenolik, fermentasi, rimpang jeringau

Isolation and Diagnosis of Candida Albicans from the Vaginal Canal and Test the Effect of Water Extract of Turmeric Inhibitor for Growth outside the Organism

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Safaa Sultan Alhadedy ◽  
Maha Irzoqy Al-Doory
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Agent ◽  
Water Extract ◽  
Growth Effect ◽  
Main Study ◽  
Turmeric Extract ◽  
Inflammatory Drugs ◽  
Vaginal Canal ◽  
Growth Of Fungi ◽  
Herbal Plant

The curcumin found in the herbal plant Turmeric is the active ingredient for the treatment of many diseases as they have a set of useful properties that enabled them to be used as anti-inflammatory drugs and also used as antioxidants and based on recent studies have shown that curcumin is influential in the growth of fungi and bacteria was the goal. The main study of the current study is to test the effect of curcumin in the growth of yeast albicans Candida has been used in this research one way is the method of tablets soaked Turmeric extract water for two periods of time (2 hours - 24 hours) and then took the results that showed that turmeric has an anti-growth effect albicans Candida and increases this effect by increasing concentrations and duration of soaking. We can conclude that the turmeric extract can be used as an antimicrobial agent for the growth of vaginal albicans Candida.

scholarly journals Defining pheromone-receptor signaling inCandida albicansand related asexualCandidaspecies

2011 ◽  
Vol 22 (24) ◽  
pp. 4918-4930 ◽  
Author(s):  
Ching-Hsuan Lin ◽  
Anthony Choi ◽  
Richard J. Bennett
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Cellular Response ◽  
Fungal Species ◽  
The Novel ◽  
Pheromone Receptor ◽  
Human Fungal Pathogen ◽  
Α Pheromone ◽  
Candida Lusitaniae ◽  
Sexual Mating

Candida albicans is an important human fungal pathogen in which sexual reproduction is under the control of the novel white–opaque switch. Opaque cells are the mating-competent form, whereas white cells do not mate but can still respond to pheromones, resulting in biofilm formation. In this study, we first define the domains of the α-pheromone receptor Ste2 that are necessary for signaling in both white and opaque forms. Both cell states require the IC loop 3 (IC3) and the C-terminal tail of Ste2 for the cellular response, whereas the first IC loop (IC1) of Ste2 is dispensable for signaling. To also address pheromone-receptor interactions in related species, including apparently asexual Candida species, Ste2 orthologues were heterologously expressed in Candida albicans. Ste2 receptors from multiple Candida clade species were functional when expressed in C. albicans, whereas the Ste2 receptor of Candida lusitaniae was nonfunctional. Significantly, however, expression of a chimeric C. lusitaniae Ste2 receptor containing the C-terminal tail of Ste2 from C. albicans generated a productive response to C. lusitaniae pheromone. This system has allowed us to characterize pheromones from multiple Candida species and indicates that functional pheromone-receptor couples exist in fungal species that have yet to be shown to undergo sexual mating.

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