Serum concentrations of 10 acute-phase proteins in healthy term and preterm infants from birth to age 6 months

Abstract Aiming to define the evolution pattern of 10 acute-phase proteins in early infancy, we measured nephelometrically the serum concentrations of albumin, prealbumin, retinol-binding protein, transferrin, ceruloplasmin, hemopexin, haptoglobin, alpha 1-acid glycoprotein, alpha 2-macroglobulin, and alpha 1-antitrypsin in 395 term and preterm infants (gestational ages 26-41 weeks). Measurements were performed within 24 h after birth and then at the end of 1 (n = 171), 3 (n = 155), and 6 (n = 90) months afterwards. Data obtained from 250 healthy adults were used as adult reference values. All proteins increased progressively with postnatal age, except for alpha 1-antitrypsin, which remained stable from birth to the 6th month. Concentrations of almost all measured proteins were significantly lower in preterm than in term infants in the first 3 months. Compared with adult values, alpha 2-macroglobulin and alpha 1-antitrypsin were higher in infants throughout the 6 months. The other proteins were significantly lower at birth than adult values but after 6 months, only albumin, prealbumin, retinol-binding protein, and alpha 1-acid glycoprotein still remained lower in infants. Thus both gestational and postnatal age should be considered when interpreting concentrations of these proteins in early infancy.

Download Full-text

Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

Clinical Chemistry ◽

10.1093/clinchem/32.5.743 ◽

1986 ◽

Vol 32 (5) ◽

pp. 743-747 ◽

Cited By ~ 13

Author(s):

J Braun ◽

T Schultek ◽

K F Tegtmeier ◽

A Florenz ◽

C Rohde ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Immunoglobulin A ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Coefficients Of Variation ◽

Alpha 1 Antitrypsin ◽

Heel Prick ◽

Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

Download Full-text

Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-7965 ◽

2016 ◽

Vol 68 (4) ◽

pp. 865-872 ◽

Cited By ~ 4

Author(s):

T.G. Rocha ◽

F.D.F. Silva ◽

C. Bortoletto ◽

D.G. Silva ◽

M.G. Buzinaro ◽

...

Keyword(s):

Acute Phase ◽

Serum Proteins ◽

Acute Phase Proteins ◽

Clinical Signs ◽

Least Square ◽

Serum Concentrations ◽

Fecal Samples ◽

Acid Glycoprotein ◽

Group B ◽

Rotavirus Diarrhea

ABSTRACT The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and α1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves.

Download Full-text

Serum concentrations of acute phase proteins in goats and ewes with naturally acquired Staphylococcus aureus mastitis

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-8722 ◽

2017 ◽

Vol 69 (2) ◽

pp. 285-292 ◽

Cited By ~ 5

Author(s):

K.M.M.G. Simplício ◽

T.G. Rocha ◽

D.C.C. Sanchez ◽

F.S. Cotrim ◽

P.C. Silva ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Serum Protein ◽

Acute Phase ◽

Acute Phase Proteins ◽

Blood Sampling ◽

Serum Concentrations ◽

Acid Glycoprotein ◽

Sds Page ◽

Page Electrophoresis

ABSTRACT Serum protein concentrations, including acute phase proteins (APPs), of goats and ewes with naturally acquired Sthaphylococcus aureus mastitis were determined by means of SDS-PAGE electrophoresis to evaluate the relevance of these APPs as biomarkers of the disease in these species. Fifteen healthy goats and 5 goats with naturally acquired staphylococci mastitis, as well as fifteen healthy ewes and 5 ewes with staphylococci mastitis were submitted to daily blood sampling during 7 days. In goats, an increase of 570%, 125%, 621%, and 279% in serum concentrations of ceruloplasmin, fibrinogen, haptoglobin and α1-acid glycoprotein, respectively, was observed. In sheep the increase in serum concentrations of ceruloplasmin, fibrinogen, haptoglobin and α1-acid glycoprotein was of 337%, 90%, 461%, and 225%, respectively. Our results indicate that these APPs have considerable potencial as early and sensible biomarkers of mastitis caused by S. aureus in goats and sheep.

Download Full-text

Serum concentrations of some acute phase proteins in cats with anaemia

Medycyna Weterynaryjna ◽

10.21521/mw.5998 ◽

2018 ◽

Vol 74 (1) ◽

pp. 5998-2018

Author(s):

GÜLTEN EMEK TUNA ◽

CEREN DINLER ◽

GAMZE SEVRI EKREN AŞICI ◽

BÜLENT ULUTAŞ

Keyword(s):

Haemolytic Anaemia ◽

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Serum Amyloid ◽

Serum Concentrations ◽

Healthy Group ◽

Acid Glycoprotein ◽

Amyloid A ◽

Serum Haptoglobin

Serum concentrations of acute phase proteins can provide valuable diagnostic information in the detection and monitoring of disease. The available information on the acute phase response in cats with anaemia is limited. The aim of this study was to retrospectively evaluate serum concentrations of haptoglobin, serum amyloid A, α1 acid glycoprotein and their clinical importance in cats with anaemia. Thirty-four anaemic cats and ten healthy cats were enrolled this study. After individual diagnoses had been established, the cats were divided into three groups (healthy group, haemolytic group and non-haemolytic group). Serum acute phase protein concentrations were analysed using specific commercially available test kits in an ELISA reader device. Serum amyloid A and serum α1 acid glycoprotein concentrations were significantly higher in the anaemic groups compared with the healthy group. Haptoglobin concentrations were significantly higher in cats from the non-haemolytic anaemia group than they were in healthy animals and those from the haemolytic anaemia group. Although serum haptoglobin concentrations were lower than in the healthy group, there was no significant difference between the haemolytic anaemia group and the healthy group. The results of this study suggest that serum amyloid A and α1 acid glycoprotein could be useful in the diagnosis and determination of inflammation in cats with anaemia. Serum haptoglobin depletion may be used for diagnosis of haemolysis in cats with haemolytic anaemia. In addition, this study has contributed to the limited data available on acute phase protein concentrations in cats with anaemia..

Download Full-text

A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence

Dalton Transactions ◽

10.1039/c2dt30143a ◽

2012 ◽

Vol 41 (42) ◽

pp. 13154 ◽

Cited By ~ 30

Author(s):

Rachel Carr ◽

Lorenzo Di Bari ◽

Samuele Lo Piano ◽

David Parker ◽

Robert D. Peacock ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Acid Glycoprotein ◽

Europium Luminescence ◽

Alpha 1 Antitrypsin

Download Full-text

The Acute Phase Proteins Reaction in Children Suffering from Pseudocroup

Mediators of Inflammation ◽

10.1155/2019/6518308 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Beata Pucher ◽

Magdalena Sobieska ◽

Michal Grzegorowski ◽

Jaroslaw Szydlowski

Keyword(s):

Acute Phase ◽

Inflammatory Reaction ◽

Acute Phase Proteins ◽

Control Group ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Persistent Inflammation ◽

Alpha 1 Antitrypsin

The aim of the study was to evaluate the inflammatory reaction in children with pseudocroup and compare it with other laryngological diseases according to the available literature data. The study group included 51 children hospitalized because of pseudocroup. The measurements of the acute phase proteins (APP), such as C-reactive protein (CRP), alpha-1-antitrypsin (AT), alpha-1-antichymotrypsin (ACT), alpha-1-acid glycoprotein (AGP), ceruloplasmin (Cp), transferrin (Tf), alpha-2-macroglobulin (A2M), and haptoglobin (Hp) were obtained at 3 time points. The glycosylation profiles of AGP, ACT, and Tf were completed. An increased AGP level was observed in girls. The AGP glycosylation revealed the advantage of the W0 variant over the W1 variant. W1 and W2 were decreased in boys. W3 emerged in boys. The Tf concentration and T4 variant were lower compared to the control group. The A2M level was lower after treatment. The Hp and AT levels were decreased a few weeks later. The ACT glycosylation revealed a decrease of the A4 variant in boys. In conclusion, the inflammatory reaction during pseudocroup was of low intensity. The APP glycosylation suggested a chronic process. In a follow-up investigation, no normalization of the parameters was noted, but signs of persistent inflammation were observed.

Download Full-text

Association of Retinol-Binding Protein 4 with Arteriovenous Fistula Dysfunction in Hemodialysis Patients

Blood Purification ◽

10.1159/000513418 ◽

2021 ◽

pp. 1-8

Author(s):

Yuanhao Wu ◽

Fan Wang ◽

Tingting Wang ◽

Yin Zheng ◽

Li You ◽

...

Keyword(s):

Risk Factor ◽

Arteriovenous Fistula ◽

Independent Risk Factor ◽

Binding Protein ◽

Retinol Binding Protein ◽

Youden Index ◽

Serum Concentrations ◽

Retinol Binding Protein 4 ◽

Kaplan Meier ◽

Increased Risk

Background: Arteriovenous fistula (AVF) is the most common vascular access for patients undergoing hemodialysis (HD). Neointimal hyperplasia (NIH) might be a potential mechanism of AVF dysfunction. Retinol-binding protein 4 (RBP4) may play an important role in the pathogenesis of NIH. The aim of this study was to investigate whether AVF dysfunction is associated with serum concentrations of RBP4 in HD subjects. Methods: A cohort of 65 Chinese patients undergoing maintenance HD was recruited between November 2017 and June 2019. The serum concentrations of RBP4 of each patient were measured with the ELISA method. Multivariate logistic regression was used to analyze data on demographics, biochemical parameters, and serum RBP4 level to predict AVF dysfunction events. The cutoff for serum RBP4 level was derived from the highest score obtained on the Youden index. Survival data were analyzed with the Cox proportional hazards regression analysis and Kaplan-Meier method. Results: Higher serum RBP4 level was observed in patients with AVF dysfunction compared to those without AVF dysfunction events (174.3 vs. 168.4 mg/L, p = 0.001). The prevalence of AVF dysfunction events was greatly higher among the high RBP4 group (37.5 vs. 4.88%, p = 0.001). In univariate analysis, serum RBP4 level was statistically significantly associated with the risk of AVF dysfunction (OR = 1.015, 95% CI 1.002–1.030, p = 0.030). In multivariate analysis, each 1.0 mg/L increase in RBP4 level was associated with a 1.023-fold-increased risk of AVF dysfunction (95% CI for OR: 1.002–1.045; p = 0.032). The Kaplan-Meier survival analysis indicated that the incidence of AVF dysfunction events in the high RBP4 group was significantly higher than that in the low-RBP4 group (p = 0.0007). Multivariate Cox regressions demonstrated that RBP4 was an independent risk factor for AVF dysfunction events in HD patients (HR = 1.015, 95% CI 1.001–1.028, p = 0.033). Conclusions: HD patients with higher serum RBP4 concentrations had a relevant higher incidence of arteriovenous dysfunction events. Serum RBP4 level was an independent risk factor for AVF dysfunction events in HD patients.

Download Full-text

Experimental Ehrlichia canis infection changes acute-phase proteins

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612012000300006 ◽

2012 ◽

Vol 21 (3) ◽

pp. 206-212 ◽

Cited By ~ 13

Author(s):

Thiago Demarchi Munhoz ◽

Joice Lara Maia Faria ◽

Giovanni Vargas-Hérnandez ◽

José Jurandir Fagliari ◽

Áureo Evangelista Santana ◽

...

Keyword(s):

Early Detection ◽

Early Diagnosis ◽

Acute Phase ◽

Acute Phase Proteins ◽

Ehrlichia Canis ◽

Laboratory Findings ◽

Early Detection Of Disease ◽

Acid Glycoprotein ◽

The Third ◽

Canine Ehrlichiosis

Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.

Download Full-text