scholarly journals INHIBITION OF GROWTH BY AMBER SUPPRESSORS IN YEAST

Genetics ◽  
1976 ◽  
Vol 82 (2) ◽  
pp. 233-249
Author(s):  
Susan W Liebman ◽  
Fred Sherman

ABSTRACT Strains of the yeast Saccharomyces cerevisiae that contain highly efficient amber (UAG) suppressors grow poorly on nutrient medium, while normal or nearly normal growth rates are observed when these strains lose the suppressors or when the suppressors are mutated to lower efficiencies. The different growth rates account for the accumulation of mutants with lowered efficiencies in cultures of strains with highly efficient amber suppressors. Genetic analyses indicate that one of the mutations with a lowered efficiency of suppression is caused by an intragenic mutation of the amber suppressor. The inhibition of growth caused by excessive suppression is expected to be exacerbated when appropriate suppressors are combined together in haploid cells if two suppressors act with a greater efficiency than a single suppressor. Such retardation of growth is observed with combinations of two UAA (ochre) suppressors (Gilmore 1967) and with combinations of two UAG suppressors when the efficiencies of each of the suppressors are within a critical range. In contrast, combinations of a UAA suppressor and a UAG suppressor do not affect growth rate. Apparently while either excessive UAA or excessive UAG suppression is deleterious to yeast, a moderate level of simultaneous UAA and UAG suppression is not.

Genetics ◽  
1978 ◽  
Vol 89 (4) ◽  
pp. 653-665
Author(s):  
Arjun Singh ◽  
Fred Sherman

ABSTRACT Some of the deletions in the yeast Saccharomyces cerevisiae that encompass the CYC1 gene, which determines iso-1-cytochrome c, extend into the OSM1 gene, causing inhibition of growth on hypertonic media, and into the RAD7 gene, causing sensitivity to UV light. Two deletions (cyc1-363 and cyc1-367) encompass only the CYC1 gene, two deletions (cyc1-366 and cyc1-368) encompass the CYC1 and OSM1 genes, three deletions (cyc1-1, cyc1-364 and cyc1-365) encompass the CYC1, OSM1 and RAD7 genes, while none of the deletions extend into the closely linked SUP4 gene.


Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1147-1156 ◽  
Author(s):  
Theodor Hanekamp ◽  
Mary K Thorsness ◽  
Indrani Rebbapragada ◽  
Elizabeth M Fisher ◽  
Corrine Seebart ◽  
...  

Abstract In the yeast Saccharomyces cerevisiae, certain mutant alleles of YME4, YME6, and MDM10 cause an increased rate of mitochondrial DNA migration to the nucleus, carbon-source-dependent alterations in mitochondrial morphology, and increased rates of mitochondrial DNA loss. While single mutants grow on media requiring mitochondrial respiration, any pairwise combination of these mutations causes a respiratory-deficient phenotype. This double-mutant phenotype allowed cloning of YME6, which is identical to MMM1 and encodes an outer mitochondrial membrane protein essential for maintaining normal mitochondrial morphology. Yeast strains bearing null mutations of MMM1 have altered mitochondrial morphology and a slow growth rate on all carbon sources and quantitatively lack mitochondrial DNA. Extragenic suppressors of MMM1 deletion mutants partially restore mitochondrial morphology to the wild-type state and have a corresponding increase in growth rate and mitochondrial DNA stability. A dominant suppressor also suppresses the phenotypes caused by a point mutation in MMM1, as well as by specific mutations in YME4 and MDM10.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Kanhaiya Kumar ◽  
Vishwesh Venkatraman ◽  
Per Bruheim

Abstract Background Saccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the heterologous production of commercially interesting small molecules and proteins. The central metabolism is at the core to provide building blocks and energy to support growth and survival in normal situations as well as during exogenous stresses and forced heterologous protein production. Here, we present a comprehensive study of intracellular central metabolite pool profiling when growing S. cerevisiae on different carbon sources in batch cultivations and at different growth rates in nutrient-limited glucose chemostats. The latest versions of absolute quantitative mass spectrometry-based metabolite profiling methodology were applied to cover glycolytic and pentose phosphate pathway metabolites, tricarboxylic acid cycle (TCA), complete amino acid, and deoxy-/nucleoside phosphate pools. Results Glutamate, glutamine, alanine, and citrate were the four most abundant metabolites for most conditions tested. The amino acid is the dominant metabolite class even though a marked relative reduction compared to the other metabolite classes was observed for nitrogen and phosphate limited chemostats. Interestingly, glycolytic and pentose phosphate pathway (PPP) metabolites display the largest variation among the cultivation conditions while the nucleoside phosphate pools are more stable and vary within a closer concentration window. The overall trends for glucose and nitrogen-limited chemostats were increased metabolite pools with the increasing growth rate. Next, comparing the chosen chemostat reference growth rate (0.12 h−1, approximate one-fourth of maximal unlimited growth rate) illuminates an interesting pattern: almost all pools are lower in nitrogen and phosphate limited conditions compared to glucose limitation, except for the TCA metabolites citrate, isocitrate and α-ketoglutarate. Conclusions This study provides new knowledge-how the central metabolism is adapting to various cultivations conditions and growth rates which is essential for expanding our understanding of cellular metabolism and the development of improved phenotypes in metabolic engineering.


2020 ◽  
Author(s):  
James Diana

Abstract Efficient supplementary feeding will utilize not only the feed added to the pond, but also natural food. Feeding improves the growth rate of fish in a pond only when the fish are food limited. Natural foods may provide all of the necessary nutrition for normal growth of small fish, and if growth begins to decline at a larger size, supplementary feeding should yield higher growth rates. One experiment compared the use of fertilizer alone, feed alone, and a combination of feed and fertilizer for growth and production of Nile tilapia (Oreochromis niloticus). Ponds receiving feed and fertilizer had higher growth rates than ponds with feed alone, and both showed considerably higher growth rate than ponds with fertilizer alone. Feeding was necessary to grow fish to a size of over 200 g in a reasonable amount of time. A second experiment tested the possibility that feeding at reduced ration and allowing the consumption of natural food might give a comparable growth rate to feeding alone. In this experiment, full satiation ration, 75% satiation ration, or even 50% satiation ration resulted in similar mean weights at harvest, about 400 g after 150 days. These results indicate that it may be efficient to simply feed the fish at about half of their maximum ration and allow them to eat natural food for the other half of their growth, if the pond is managed well. Economic analysis showed that fertilization resulted in $1,891 ha-1 year-1 of profit, feed only lost almost $3,000 ha-1 year-1, 75% feeding lost $258, 50% feeding yielded $1,765 ha-1 year-1, and 25% feeding yielded $155 ha-1 year-1. If the growth rate were linearly extrapolated until the fish reached 500 g and were then harvested, the 75% feeding would yield $4,835 ha-1 year-1 and the 50% feeding $5,865 ha-1 year-1. A third experiment was conducted to evaluate the timing when first feeding should occur. In this case, fish in ponds were fed once they reached 50, 100, 150, 200 or 250 g in size. The fish grew similarly before they were fed, and then grew again similarly after feeding, with a growth rate under fertilization of about 1 g day-1, and a growth rate under feeding of about 3 g day-1. Fish fed first at 50 or 100 g reached the same ultimate size by the end of the experiment and had similar production levels. Fish first fed at 150 or 200 g also showed similar results and, finally, fish fed at 250 g never reached the targeted size. These results indicate that it is most efficient to wait until the fish reach 100 g before commencing artificial feeding. Economic evaluation showed an annual net profit of $3,700 ha-1 year-1 when the fish were fed first at 50 g, $6,160 at 100 g, $4,800 at 150 g, $1,800 at 200 g and $3,600 at 250 g. Clearly, efficient feeding systems produce higher incomes than less efficient systems, and fertilization alone, if the fish reach a targeted size that has a higher economic value.


1970 ◽  
Vol 25 (9) ◽  
pp. 1020-1023 ◽  
Author(s):  
Wolfram Thiemann ◽  
Erich Wagner

The influence of strong homogeneous magnetic fields in the range of 5000 to 8000 Gauss on the growth of Saccharomyces cerevisiae and Micrococcus denitrificans was studied. In the case of yeast growing under nearly anaerobic conditions an inhibition of growth rate was observed in the beginning of incubaton while some hours later the growth accelerated and surpassed the control. M. denitrificans on the other hand grew with the same rate as the controls during the first 2 - 3 hours of experiment; thereafter the magnetic field resulted in a significant acceleration of growth rate measured by a 5.8 to 13.3% increase of oxygen consumption after 5 - 6 hours run of experiment. Until now only inhibition of bacterial growths by magnetic fields is reported elsewhere in the literature.


1978 ◽  
Vol 24 (1) ◽  
pp. 28-30 ◽  
Author(s):  
Adrian P. Wills ◽  
E. C. S. Chan

When deprived of biotin, Arthrobacter globiformis 425 exhibits abnormal morphology (large, branched forms of variable size) and a retardation of its normal growth rate. In chemostat cultures, when cells were grown under glucose limitation, the morphology was normal (coccoids or rods) at specific growth rates between 0.05 and 0.125 h−1 (doubling times between 14 and 5.5 h, respectively) at 25 °C. The coccoid-to-rod morphogenesis occurs at a specific growth rate of 0.11 h−1. At the same specific growth rates and temperature, but under biotin limitation, abnormal morphology was observed.


1993 ◽  
Vol 290 (2) ◽  
pp. 369-374 ◽  
Author(s):  
S Mandrup ◽  
R Jepsen ◽  
H Skøtt ◽  
J Rosendal ◽  
P Højrup ◽  
...  

We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.


Genetics ◽  
1994 ◽  
Vol 136 (1) ◽  
pp. 93-105
Author(s):  
G Natsoulis ◽  
F Winston ◽  
J D Boeke

Abstract Mutations in the SPT10 and SPT21 genes were originally isolated as suppressors of Ty and LTR (delta) insertion mutations in Saccharomyces cerevisiae, and the genes were shown to be required for normal transcription at a number of loci in yeast. Now we have cloned, sequenced, mapped and mutagenized SPT10 and SPT21. Since the spt10 mutation used to clone SPT10 resulted in very poor transformation efficiency, a novel method making use of the kar1-1 mutation was used. Neither SPT gene is essential for growth, and constructed null alleles cause phenotypes similar to those caused by spontaneous mutations in the genes. spt10 null alleles are strong suppressor mutations and cause extremely slow growth. Certain spt10 spontaneous alleles are good suppressors but have a normal growth rate, suggesting that the SPT10 protein may have two distinct functions. An amino acid sequence motif that is similar to the Zn-finger motif was found in SPT10. Mutation of the second Cys residue in this motif resulted in loss of complementation of the suppression phenotype but a normal growth rate. Thus, this motif may reside in a part of the SPT10 protein that is important for transcriptional regulation but not for normal growth. Both the SPT10 and SPT21 proteins are relatively tolerant of large deletions; in both cases deletions of the C-terminus resulted in at least partially functional proteins; also, a large internal deletion in SPT21 was phenotypically wild type.


Author(s):  
T. V. Meledina ◽  
V. A. Ivanova ◽  
H. Razan ◽  
O. V. Golovinskaya ◽  
I. V. Novikova ◽  
...  

The growth and multiplication of the industrially significant yeast Saccharomyces cerevisiae is primarily determined by the balance of the composition of the nutrient medium used. In order to prevent a decrease in the speed of the biotechnology process and to achieve the optimal yield of the desired biosynthesis product (biomass), it is necessary to introduce such nutrient deficiencies as vitamins and trace elements into the nutrient medium. At present, there is much information about the influence of different environmental factors on the growth and multiplication of microorganisms. However, the potential possibilities of microbial cultures have not been fully used. Researchers during the preparation of nutrient media until recent time mainly use the methods of establishing one-factor dependence that are based on the principle of alternating change of each nutrient media factor with the others being constant. In this work, the impact on the biomass yield of various process parameters in their interactions is investigated. A multifactor dependence is established using the methods of mathematical design of an experiment. These methods allow both to study the effects of a large number of factors and to construct a mathematical model of the process revealing the quantitative value of each individual factor and to take into account the interfactor interactions in the system .The cultivation of the yeast Saccharomyces cerevisiae was conducted in a simple periodic culture. The factors used were: the amount of inoculum, the content of nitrogen, phosphorus and biotin in the medium. Using experimental data and multifactor analysis, it was found that under these conditions, the content of biotin in the medium mostly affects the biomass synthesis. It was established that, in an optimally composed media, the economic coefficient was independent of the size of the seeding. Moreover, it was shown that the size of the fund of free amino acids is inversely related to the consumption of the seeding: with an increase in the initial density of the population, the number of amino acids in cells decreases.


2019 ◽  
Vol 18 (3) ◽  
pp. 9-15
Author(s):  
Mary Ann Keogh Hoss, PhD, CTRS, FACHE, FDRT

This article explores why there is a decline in the growth rate of recreational therapy while there are substantial increases in other therapy professions and recreation workers. The growth numbers of the recreational therapy profession are at a normal growth rate of 7 percent for all occupations according to the Bureau of Labor Statistics (BLS). The growth rate from the Occupational Handbook for 2010-2011 projected a growth rate of 15 percent. The growth rates for occupational therapy, “therapists, all others” and recreation workers are higher than recreational therapy projected by BLS at 20 percent or higher. Possible considerations as to causes for this change in growth are proposed.


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