Providencia stuartii: a review of a multiply antibiotic-resistant bacterium

Ultraviolet disinfection could effectively inactivate the antibiotic resistant bacterium vancomycin resistant Enterococcus faecalis, but had a limited removal efficiency for the antibiotic resistance gene–vanB gene.

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Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model

Antibiotics ◽

10.3390/antibiotics10040375 ◽

2021 ◽

Vol 10 (4) ◽

pp. 375

Author(s):

Chani Rakov ◽

Shira Ben Porat ◽

Sivan Alkalay-Oren ◽

Ortal Yerushalmy ◽

Mohanad Abdalrhman ◽

...

Keyword(s):

Urinary Tract ◽

Urinary Tract Infections ◽

Phage Therapy ◽

Multiple Drug ◽

Bacterial Strains ◽

Antibiotic Resistant ◽

Isolation And Characterization ◽

Wide Range ◽

Providencia Stuartii ◽

Tract Infections

Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular.

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Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii

Frontiers in Microbiology ◽

10.3389/fmicb.2021.742114 ◽

2021 ◽

Vol 12 ◽

Author(s):

Dan Wang ◽

Hong Li ◽

Xiang Ma ◽

Yanqiong Tang ◽

Hongqian Tang ◽

...

Keyword(s):

Stress Resistance ◽

Efflux Pump ◽

Wild Type ◽

Antibiotic Resistant ◽

Zoonotic Pathogen ◽

Transcriptomic Data ◽

Aeromonas Veronii ◽

Metabolic Genes ◽

Antibiotic Resistant Bacterium ◽

Growth Metabolism

Aeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage Qβ replication) participates in the regulations of the virulence, adhesion, and nitrogen fixation, effecting on the growth, metabolism synthesis and stress resistance in bacteria. The deletion of hfq gene in A. veronii showed more sensitivity to trimethoprim, accompanying by the upregulations of purine metabolic genes and downregulations of efflux pump genes by transcriptomic data analysis. Coherently, the complementation of efflux pump-related genes acrA and acrB recovered the trimethoprim resistance in Δhfq. Besides, the accumulations of adenosine and guanosine were increased in Δhfq in metabonomic data. The strain Δhfq conferred more sensitive to trimethoprim after appending 1 mM guanosine to M9 medium, while wild type was not altered. These results demonstrated that Hfq mediated trimethoprim resistance by elevating efflux pump expression and degrading adenosine, and guanosine metabolites. Collectively, Hfq is a potential target to tackle trimethoprim resistance in A. veronii infection.

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Total synthesis of the O-antigen repeating unit of Providencia stuartii O49 serotype through linear and one-pot assemblies

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.17.199 ◽

2021 ◽

Vol 17 ◽

pp. 2915-2921

Author(s):

Tanmoy Halder ◽

Somnath Yadav

Keyword(s):

Total Synthesis ◽

Pathogenic Bacteria ◽

Capsular Polysaccharides ◽

One Pot ◽

Antibiotic Resistant ◽

O Antigen ◽

The Family ◽

Providencia Stuartii ◽

The One ◽

Tract Infections

Capsular polysaccharides of pathogenic bacteria have been reported to be effective vaccines against diseases caused by them. Providencia stuartii is a class of enterobacteria of the family Providencia that is responsible for several antibiotic resistant infections, particularly urinary tract infections of patients with prolonged catheterization in hospital settings. Towards the goal of development of vaccine candidates against this pathogen, we herein report the total synthesis of a trisaccharide repeating unit of the O-antigen polysaccharide of the P. stuartii O49 serotype containing the →6)-β-ᴅ-Galp-(1→3)-β-ᴅ-GalpNAc(1→4)-α-ᴅ-Galp(1→ linkage. The synthesis of the trisaccharide repeating unit was carried out first by a linear strategy involving the [1 + (1 + 1 = 2)] assembly, followed by a one-pot synthesis involving [1 + 1 + 1] strategy from the corresponding monosaccharides. The one-pot method provided a higher yield of the protected trisaccharide intermediate (73%) compared to the two step synthesis (66%). The protected trisaccharide was then deprotected and N-acetylated to finally afford the desired trisaccharide repeating unit as its α-p-methoxyphenyl glycoside.

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PREVALENCE OF MULTIPLE ANTIBIOTIC RESISTANT AND HAEMOLYSIN PRODUCING BACTERIA IN MARKETED FISH SAMPLES

Kongunadu Research Journal ◽

10.26524/krj239 ◽

2017 ◽

Vol 4 (3) ◽

pp. 94-97

Author(s):

Subashkumar R

Keyword(s):

Food Poisoning ◽

Developed Countries ◽

Resistance Pattern ◽

Antibiotic Resistant ◽

Food Borne ◽

Fish Samples ◽

Antibiotic Resistant Bacterium ◽

Mar Index ◽

Future Demands ◽

Excellent Source

Sea foods hold one of the greatest potentials to meet current and future demands of proteins to feed the world’s ever-burgeoning population. Fresh seafood is an excellent source of proteins, a good source of minerals, and some vitamins, and it is low in fats, cholesterol, and sodium. Fishery products which are of great importance for human nutrition worldwide and provide clear health benefits. Food borne pathogens are the leading causes of illness and death in less developed countries killing approximately 1.8 million people annually. Bacteria are the most important cause of seafood spoilage. Percentage prevalence of bacterial population in fish samples collected from Ukkadam fish market, Coimbatore was significantly higher during the study period. About 7 fish intestinal samples were then enriched in nutrient broth and cultured. Biochemical test were performed to determine their phenotypic characteristics. The antibiotic resistance pattern and MAR index showed an increased antibiotic resistant bacterium in fish which may severe food borneillness in human. Thehemolytic activity and extracellular protein reveals the frequency of virulence and strong pathogenecity. On this basis, we came to the conclusion that all the isolates are highly pathogenic and cause various food poisoning in humans.

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