scholarly journals 186 Hormone content of anabolic implants differentially affects plasma and liver trace mineral concentrations

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 154-155
Author(s):  
Elizabeth M Messersmith ◽  
Caleb C Reichhardt ◽  
Kara J Thornton ◽  
Stephanie L Hansen

Abstract Forty-eight commercial Angus steers (327 ± 25 kg) were utilized to determine effects of varying implant hormone composition on liver and plasma trace mineral concentrations. Implant (IMP) treatments (n = 12/treatment) included: no implant (CON), estradiol (E2; Compudose; 25.7 mg estradiol; Elanco Animal Health; Greenfield, IN), trenbolone acetate (TBA; Finaplix H; 200 mg trenbolone acetate; Merck Animal Health; Madison, NJ), and a combination of estradiol and trenbolone acetate (ETBA Revalor-S; 24 mg estradiol + 120 mg trenbolone acetate; Merck Animal Health). Cattle were fed forage-based step up rations throughout the sampling period. Plasma samples were collected on d 0, 2, 10 and 30, with liver biopsies collected on d 2 and 10. Data were analyzed with ProcMixed of SAS with fixed effect of IMP. Contrast statements for each treatment vs CON were developed. Plasma trace mineral analysis utilized repeated measures (repeated effect = day). No IMP × Day effects (P ≥ 0.18) on plasma trace mineral were observed. Day 2 liver Zn and Cu and d 10 liver Mn were lesser (P ≤ 0.04) for TBA implanted steers than CON and d 2 liver Mn tended to be lesser (P = 0.06). The Mn response may reflect changes in protein metabolism due to TBA. However, E2 and ETBA tended to have greater (P ≤ 0.07) d 10 liver Fe concentrations than CON while plasma Fe was unaffected (P ≥ 0.75) by E2, TBA, or ETBA. Both d 2 liver and plasma Zn were lesser for ETBA than CON (P ≤ 0.04) likely driven by the TBA in the implant. Implant contrasts did not affect (P ≥ 0.13) other plasma or liver trace mineral concentrations. These data indicate implant strategies, and thus hormones differentially influence trace mineral metabolism in feedlot steers, which may have implications for diet formulation.

Author(s):  
Elizabeth M Messersmith ◽  
Stephanie L Hansen

Abstract Seventy-two Angus-crossbred steers (411 ± 16 kg) were assigned to a 2 × 3 factorial arrangement of treatments to examine the effects of blended Zn source supplementation on performance, carcass characteristics, and trace mineral parameters of steers administered no implant or a two-implant program. Factors included implant (IMP) strategies and Zn supplementation. During the 126-d study steers were either not implanted (NoIMP) or implanted (IS/200; Elanco, Greenfield, IN) on d 0 (Component TE-IS; 80 mg trenbolone acetate + 16 mg estradiol) and 57 (Component TE-200; 200 mg trenbolone acetate + 20 mg estradiol). All steers were fed 70 mg Zn/kg on a dry matter (DM) basis from ZnSO4 + 30 mg Zn/kg DM from either basic ZnCl (Vistore Zn, Phibro Animal Health, Teaneck, NJ), Zn glycinate (Gemstone Zn, Phibro Animal Health), or ZnSO4 (ZnB, ZnG, or ZnS, respectively). Steers were blocked by weight into pens of 6 and fed a dry rolled corn-based diet via GrowSafe bunks (GrowSafe Systems Ltd.; Airdrie, AB, Canada). Data were analyzed using the Mixed Procedure of SAS, with fixed effects of Zn, IMP, and the interaction. Steer was the experimental unit (n = 12 steers/treatment). Liver and muscle collected on d -5, 14, 71, and 120 were analyzed for Zn concentration, and data were analyzed as repeated measures (repeated effect = Day). An IMP × Zn tendency (P = 0.07) was observed for d 126 body weight with no effects of Zn within NoIMP while ZnS tended to be heavier than ZnB with ZnG intermediate within IS/200. Carcass-adjusted overall feed efficiency (G:F) was greatest for ZnS (Zn; P = 0.02). Implanted cattle had greater DM intake, G:F, and carcass-adjusted performance (P ≤ 0.01). Liver Zn concentrations were greater for IS/200 by d 120 (IMP × Day; P = 0.02). Within IS/200, ZnG tended to have greater muscle Zn than ZnS while ZnB was intermediate (Zn × IMP; P = 0.09). No Zn or IMP × Zn (P ≥ 0.12) effects were observed for carcass data. However, IS/200 had greater hot carcass weight, dressing percentage, and ribeye area than NoIMP (P ≤ 0.001). These data suggest implants improve growth and influence Zn metabolism. Future work should examine Zn sources and supplementation alongside implant strategies.


Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1964
Author(s):  
Caleb C. Reichhardt ◽  
Elizabeth M. Messersmith ◽  
Tevan J. Brady ◽  
Laura A. Motsinger ◽  
Reganne K. Briggs ◽  
...  

Fifty Angus-sired steers were utilized to evaluate the effects of anabolic implants varying in hormone type and concentration on performance, carcass traits, and plasma and liver trace mineral concentrations over 129 d. Steers were stratified by weight into one of four (n = 12 or 13/treatment) implant treatments: (1) estradiol (E2; 25.7 mg E2; Compudose, Elanco Animal Health, Greenfield, IN, USA), (2) trenbolone acetate (TBA; 200 mg TBA; Finaplix-H, Merck Animal Health, Madison, NJ, USA), (3) combination implant (ETBA; 120 mg TBA + 24 mg E2; Revalor-S, Merck Animal Health), or (4) no implant (CON). Steers were randomly assigned to pens equipped with GrowSafe bunks and fed a corn and barley-based finishing ration. Overall average daily gain and body weight were greater for ETBA and TBA than CON (p ≤ 0.04), but not E2 (p ≥ 0.12). Feed efficiency and hot carcass weight were only greater than CON for ETBA (p ≤ 0.03). Plasma and d 2 liver Zn concentrations were lesser for ETBA than CON (p ≤ 0.01) and d 10 liver Mn was lesser (p = 0.0003) for TBA than CON. These data indicate that implants containing TBA influence growth and trace mineral parameters, though more work investigating this relationship is necessary.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 80-81
Author(s):  
Cierrah J Kassetas ◽  
Joel Caton ◽  
James D Kirsch ◽  
Sheri T Dorsam ◽  
Kacie L McCarthy ◽  
...  

Abstract Thirty-six half-sibling yearling Angus bulls [256 ± 8 d; initial BW = 320 ± 2 kg] were assigned one of three diets: 1) 60% concentrate as corn (CON; n = 12); 2) 60% DDGS (60DDGS; n = 12); 3) CON diet + equivalent sulfur of 60DDGS as CaSO4 (SULF; n = 12) to evaluate effects of feeding 60% DDGS or sulfur as CaSO4 on trace mineral (TM) concentrations in serum and seminal plasma. Bulls were fed in Calan gates for 112 days and targeted to gain 1.6 kg/d. Data were analyzed as repeated measures using PROC MIXED in SAS. In serum, treatment × day interactions were observed (P ≤ 0.03) for Cu, Se, and Mo. For Cu, no differences (P > 0.15) were observed at d 0 or 56, but at d 112, 60DDGS was reduced (P < 0.01) compared with SULF and CON. At d 0, no differences (P > 0.09) were observed for Se; however, at d 56 and 112, 60DDGS was greater (P < 0.01) than CON and SULF. For Mo, at d 0, 60DDGS was greater (P = 0.03) than CON, whereas SULF was intermediate, but at d 56 and 112, CON was greater (P < 0.01) than SULF and 60DDGS. In seminal plasma, treatment × day interactions were observed (P ≤ 0.02) for Cu and Mo. For Cu, no differences (P ≥ 0.09) were observed at d 0 or 56, but at d 112, CON and 60DDGS were greater (P < 0.01) compared with SULF. For Mo, at d 0, 60DDGS was greater (P = 0.03) than SULF, whereas CON was intermediate, but on d 56 and 112, CON was greater (P < 0.01) than 60DDGS and SULF. Differences observed for TM may have influenced enzyme activity and semen kinematics which were previously reported.


2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John J Wagner ◽  
Jerry W Spears ◽  
Terry Eugene Engle

Abstract Twelve Angus steers (BW 452.8 ± 6.1 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral (TM) source on digestibility, ruminal volatile fatty acid (VFA) composition, ruminal soluble concentrations of Cu, Zn, and Mn, and relative binding strength of trace minerals located in the rumen insoluble digesta fraction. Steers were fed a medium-quality grass hay diet (DM basis: 10.8% CP, 63.1% neutral detergent fiber [NDF], 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment) to provide 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively. Following a 21-d adaptation period, total fecal output was collected for 5 d. Dry matter (P < 0.07) and CP (P < 0.06) digestibility tended to be reduced, and NDF (P < 0.04) and acid detergent fiber (ADF) (P < 0.05) digestibility were reduced in STM- vs. HTM-supplemented steers. On day 6, ruminal fluid was collected at 0, 2, and 4 h post-feeding and analyzed for VFA. There were no treatment x time interactions for VFA. Steers receiving HTM had less (P < 0.02) molar proportions of butyric acid and greater (P < 0.05) total VFA concentrations than STM-supplemented steers. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On day 15 steers received a pulse dose of 20 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either STM or HTM (n = 6 steers/treatment). Ruminal samples were obtained at 2-h intervals starting at −4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.03) for ruminal soluble Cu, Mn, and Zn concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post-dosing in STM compared with HTM-supplemented steers. Copper concentrations were greater (P < 0.05) at 12 and 24 h and Zn concentrations in ruminal solid digesta were greater at 24 h in HTM-supplemented steers. Upon dialysis against Tris-EDTA, the percent Zn released from digesta was greater (P < 0.05) at 12 h (P < 0.03) and 24 h (P < 0.05), and the percent Cu released was greater (P < 0.02) at 24 h post-dosing in HTM steers when compared with STM-supplemented steers. Results indicate that Cu and Zn from HTM have low solubility in the rumen and appear to be less tightly bound to ruminal solid digesta than Cu and Zn from STM. The lower ruminal soluble concentrations of Cu and Zn in steers given HTM were associated with greater fiber digestibility.


2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 12-13
Author(s):  
Jordan Adams ◽  
Rodney Farris ◽  
Scott Clawson ◽  
Earl Ward ◽  
Paul Beck

Abstract We evaluated the effects of supplementing dried distillers’ grains cubes (DDGS) and re-implantation of steers (n = 149; BW = 238 ± 13.8 kg) grazing tall fescue (Festuca arundinacea)/bermudagrass (Cynodon dactylon) pastures (n = 9 pastures, 7.2 ± 2.90 ha) from 14 April to 17 September 2020 (n = 155 d) in a split-plot design on steer performance and forage production. Main plot supplemental treatments (n = 3 pastures/treatment) included 1) Fertilized Control (FC), no supplementation on fertilized pastures (112 kg N/ha); 2) Fertilized Supplement (FS), supplemental DDGS fed at 2.9 kg 3-d/wk on fertilized pastures; and 3) Supplement (S), supplemented DDGS at 0.75% BW/d on unfertilized pastures prorated for 5-d/wk feeding. Steers were previously implanted during receiving with 40 mg trenbolone acetate and 8 mg estradiol (REV-G; Revalor G, Merck Animal Health). On July 7, steers in each pasture were randomly assigned to one of three re-implant treatments: 1) no re-implant; 2) REV-G; or 3) 200 mg progesterone and 20 mg estradiol (Synovex S, Zoetis Animal Health). Steers in FS and S gained more (P < 0.01) than FC throughout the trial and final BW was greater (P < 0.01) for FS and S compared with FC. Unexpectedly, re-implanting had no effect on ADG (P = 0.57) or BW (P = 0.34), but statistical power may be lacking. Supplemental efficiency was greater in the late summer for FS (P = 0.05) compared to S. Fertilizing pastures in FS and FC did not affect biomass (P = 0.39), however, CP was increased (P = 0.01) and acid and neutral detergent fibers tended to decrease (P = 0.06) relative to S in the early summer (April, May, June, and July), but did not differ in late summer (August and September). Based upon our analysis, DDGS is a suitable supplement and can replace N fertilizer for steers grazing introduced pastures.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 5-5
Author(s):  
Katie J Heiderscheit ◽  
Erin Deters ◽  
Alyssa Freestone ◽  
Joshua Peschel ◽  
Stephanie L Hansen

Abstract The objective was to investigate effects of 18 h feed and water restriction or transit on cattle behavior. Angus-cross steers (36; 353 ± 33 kg) were housed in pens of 6 and assigned to treatments: control (CON), full access to feed and water; deprived (DEPR), no feed or water for 18 h; or transported (TRANS), trucked for 18 h. Individual BW (n = 12 steers/treatment) was recorded on d 0, 1, 3, 8, and 14, and individual dry matter intake (DMI) was determined via GrowSafe bunks. Bunk displacements on d 1 were recorded for each pen (n = 2 pens/treatment) by one trained observer continuously for 2 h in 10 min intervals via video analysis. Steer need preferences were assessed as time individuals took to perform behaviors (eat, drink, lay) after treatments ended on d 1. Data were analyzed using Proc Mixed of SAS with fixed effect of treatment; displacements, BW, and DMI were analyzed as repeated measures. Upon return to pens, time to eat or drink did not differ between DEPR and TRANS (P ≥ 0.17), but time to lay was 70.5 min for DEPR vs. 16.5 min for TRANS (P = 0.01). Displacements were greater for DEPR than CON or TRANS during the first 90 min after accessing feed, while CON displaced more frequently than TRANS for the first 30 min (treatment × time; P = 0.02). While DMI for TRANS was not recovered until d 2, DEPR and CON had similar DMI on d 1 (treatment × day; P < 0.01). Similarly, TRANS BW were, and DEPR tended to be, lesser than CON on d 1; however, BW among treatments were not different on other days (treatment × day; P < 0.01). Thus, restricting feed increases aggressive interactions at the bunk and cattle trucked long distances are quick to lay down when allowed. These behaviors should be considered when managing an unintentional feed restriction event or receiving cattle into the feedlot.


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 46-46
Author(s):  
Zane N Grigsby ◽  
Paul A Beck ◽  
Stacey A Gunter

Abstract This research was conducted to determine effects of supplementation and implanting on BW gain by steers grazing mixed grass prairie (n = 12 pastures, 19.9 ± 0.7 ha) in northwest Oklahoma. Three main plot treatments were: 1) Negative Control (NC), no supplementation, 2) Positive Control (PC), supplemented with DDGS cubes, 1.8 kg/steer on alternate days in late summer, 3) High Supplement (HS), 1/3 increase in stocking rate with 0.75% BW supplemental DDGS cubes all season. Steers (n = 125, BW = 223.1 ± 23.2 kg) were stocked at 2.2 ha/steer for PC and NC, 1.3 ha/steer for HS. Grazing was from May 17 – September 27 (132 d). All steers were implanted with 200 mg progesterone and 20 mg estradiol benzoate (SYN, Synonvex S, Zoetis Animal Health) on May 17. On July 18 three reimplant treatments were applied: 1) no reimplant; 2) SYN; or 3) 40 mg trenbolone acetate and 8 mg estradiol (Revalor G, Merck Animal Health). Data were analyzed using the PROC MIXED in SAS as a split-plot experimental design. In early summer HS had 0.26 kg greater (P < 0.01) ADG than NC and PC. Late summer gains of PC were 0.33 kg/d more (P ≤ 0.01) than NC; and HS gained 0.49 and 0.16 kg/day more (P ≤ 0.04) than NC and PC, respectively. Gain per hectare for PC (46 kg/ha) were greater (P < 0.01) than NC (35 kg/ha) and more than doubled (P < 0.01) with HS (89 kg/ha). Reimplanting had no effect on ADG (P ≥ 0.28). Late season supplementation with PC resulted in supplemental efficiency of 2.7 kg supplement/kg added gain compared with NC. Increased stocking rates with season long supplementation in HS resulted in supplemental efficiency of 3.8 kg supplement/kg added gain per hectare. Based on these data, a 100% DDGS cube is an effective supplement option to increase BW gain during the late summer or increase carrying capacity and gain during the summer grazing period in northwestern Oklahoma.


2021 ◽  
pp. 1098612X2110262
Author(s):  
Stacie Summers ◽  
Jonathan Stockman ◽  
Jennifer A Larsen

Objectives The aim of this study was to analyze iron, copper and zinc concentrations in commercial foods and compare among food formats (dry, canned, raw), foods marketed by age category (adult 1+ years and senior 7+ years) and foods intended for adult maintenance vs all life stages. Methods In total, 112 commercial non-therapeutic food products marketed for healthy adult and senior cats were purchased in the USA. Foods were analyzed for their proximate composition. Trace mineral concentrations were measured using inductively coupled argon plasma–optical emission spectroscopy and described on a calculated metabolizable energy basis using standard modified Atwater values. Results Measured iron (median 58.4 mg/1000 kcal [range 15.7–379.0]), copper (median 5.6 mg/1000 kcal [range 0.8–13.3]) and zinc (median 47.6 mg/1000 kcal [range 7.6–128.1]) concentrations were highly variable among cat foods. When all food products – regardless of their nutritional adequacy substantiation method – were compared with the Association of American Feed Control Officials regulatory minimums, 13/112 food products had a mineral deficiency, of which a majority (n = 11/13) were raw food products. Raw foods had significantly lower trace mineral concentrations compared with dry food products and, except for copper, canned food products. Cat foods marketed for senior cats had higher iron ( P = 0.019) and zinc ( P <0.0001) concentrations than foods marketed for adult cats. Foods intended for adult maintenance had higher iron ( P = 0.003) and zinc concentrations than foods intended for all life stages ( P <0.0001). Conclusions and relevance Iron, copper and zinc concentrations in commercial non-therapeutic foods for adult and senior cats are highly variable. A minority of foods – mainly raw food products – were deficient in these minerals. It is unknown if some foods with high trace mineral concentrations could have adverse effects as studies are needed to establish safe upper limits for dietary intake of trace minerals in healthy cats.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Pedro H V Carvalho ◽  
Mariana F Westphalen ◽  
Jonathan A Campbell ◽  
Tara L Felix

Abstract The objectives of the study were to determine the effect of coated or noncoated hormone implants on growth performance, carcass characteristics, and serum estradiol-17β (E2) concentrations of Holstein steers fed a grain-based diet for 112 d. Seventy-nine Holstein steers [average initial body weight (BW) = 452 ± 5.5 kg] were stratified by BW and allotted to one of two treatments: 1) Holstein steers implanted with a coated implant containing 200 mg of trenbolone acetate (TBA) and 40 mg E2 (Revalor-XS (Merck Animal Health; Summit, NJ)] on day 0 (XS) or 2) Holstein steers implanted two times (days 0 and 56) with a noncoated implant containing 80 mg of TBA and 16 mg of E2 [(2IS) Revalor-IS (Merck Animal Health)]. Data were analyzed using the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC). There was no effect (P ≥ 0.71) of implant strategy on initial, middle, and final BW. No effect (P ≥ 0.12) of implant strategy was observed on average daily gain, dry matter intake, or gain-to-feed ratio. There were no effects (P ≥ 0.11) of implant strategy on carcass characteristics. There was an implant × day interaction (P &lt; 0.01) for the circulation of serum E2 concentrations. Serum E2 concentration increased similarly 14 d after Holstein steers were implanted, regardless of implant strategy. At 28 d, after steers were implanted, steers in the XS group had less serum E2 concentration than Holstein steers in the 2IS group. However, at 56 d after the first implantation, both groups, once again, had similar serum E2 concentrations and E2 concentrations were less on day 56 than day 28 for both strategies. Holstein steers implanted with 2IS had greater serum E2 concentration on day 70 and E2 concentrations remained greater than serum E2 of Holstein steers implanted XS for the duration of the trial (day 112). In summary, there was no effect of coated or two doses of noncoated implant on growth performance or carcass characteristics of Holstein steers.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 411-411
Author(s):  
Jun Chen ◽  
Jung Wook Lee ◽  
Jim J Monegue ◽  
Richard Niblett ◽  
Kim Williams ◽  
...  

Abstract A cooperative study evaluated effects of supplemental dietary OmniGen AF (OG; Phibro Animal Health Corporation) on reproductive characteristics of gilts. Crossbred gilts (n = 56; initial body weight = 152.3 ± 9.7 kg) were assigned to treatments in a 2 x 2 factorial design [diet (OG or control) and PG600 (Merck Animal Health, El Dora, KS) injection (Yes or No)]. Dietary treatments were: 1) corn-SBM basal diet (control) that met NRC (2012) requirement estimates, or 2) control plus 0.75% OG and were fed 35 to 40 d prior to breeding. A common diet formulation was used by both stations. Gilts were estrous-synchronized by 14-d feeding of Matrix (Merck Animal Health). PG600 injections occurred 24 hours after Matrix withdrawal. Gilts were weighed at breeding and preslaughter (at d 39 to 48 postbreeding) and were euthanized to evaluate reproductive characteristics. There were no OG x PG600 interactions on any response measures (P &gt; 0.15). Mean responses did not differ (P &gt; 0.20) when gilts were fed control or OG diets, respectively, for total uterus weight (6,065 vs. 5,883 g), uterine horn length (129.2 vs. 123.9 cm), ovary weight (9.66 vs. 9.94 g), total corpora lutea (CL) number (25.0 vs. 25.3), individual CL weight (0.41 vs. 0.43 g), total follicular fluid weight (3.17 vs. 3.03 g), individual placenta weight (86.3 vs. 86.4 g), total fetuses (14.2 vs. 13.0), and fetus crown-rump length (7.97 vs. 7.66 cm). Supplementation with OG reduced (P = 0.04) individual placenta fluid volume of gilts (150.6 vs. 115.4 mL). PG600 injection increased (P &lt; 0.001) ovary weight (9.03 vs. 10.57 g), total CL (18.09 vs 32.27) and reduced CL weight (0.46 vs. 0.38 g). The results demonstrated classic PG600 responses on reproductive characteristics but no effects of 0.75% OG supplementation nor an interaction between the two factors.


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