scholarly journals 393 Awardee Talk: Effect of trace mineral source on rumen fermentation and trace mineral distribution in the rumen

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 171-172
Author(s):  
Terry E Engle
Keyword(s):  
Small Intestine ◽  
Insoluble Fraction ◽  
Absorption Efficiency ◽  
Future Research ◽  
Microbial Fermentation ◽  
Trace Mineral ◽  
Passage Rate ◽  
Rumen Microorganisms ◽  
Fiber Digestion ◽  
Cu And Zn

Abstract The role that Cu and Zn play in rumen microbial fermentation is not well understood. Microorganisms use small proportions of dietary Cu and Zn for catalytic, structural, and stabilizing functions. In order for microorganisms to acquire Cu and Zn, the elements must be soluble in the rumen environment. Data would indicate that practical diets fed to ruminants without Cu and Zn supplementation are able to meet the microbial Cu and Zn requirements. Therefore, rumen solubility of supplemental Cu and Zn can impact rumen microbial fermentation characteristics. Numerous factors can impact rumen solubility of minerals, such as the pH of the rumen, the concentration of dietary antagonists (Mo, Fe, S, fiber, etc.), and mineral source. Earlier research has indicated that high soluble concentrations of Cu and Zn in the rumen environment can become toxic to certain rumen microorganisms and decrease fiber digestion. Recent research from our laboratory has indicated that hydroxy trace mineral (HTM) forms of Cu and Zn are relatively insoluble in the rumen and that a greater proportion of HTM remained loosely bound to the insoluble fraction within the rumen when compared to sulfate trace mineral (STM) sources of Cu and Zn. We have also reported that supplemental sources of Cu and Zn that are highly soluble in the rumen environment (STM) can decrease fiber digestion, reduce total VFA concentrations, and can become more tightly bound to rumen solid digesta than HTM sources of Cu and Zn. The stronger binding of STM relative to HTM to the solid rumen digesta fraction may reduce absorption in the small intestine. Future research investigating the flow and passage rate of different trace mineral sources through the abomasum into the duodenum and duodenal absorption efficiency of Cu and Zn from different TM sources is needed.

scholarly journals PSXII-13 The influence of trace mineral source on copper, manganese, and zinc binding strength to rumen digesta in cattle fed a high forage-based diet

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 443-443
Author(s):  
Octavio Guimaraes ◽  
Huey Yi Loh ◽  
Meghan P Thorndyke ◽  
Nicole M Tillquist ◽  
Jerry W Spears ◽  
...  
Keyword(s):  
Insoluble Fraction ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Binding Strength ◽  
Pulse Dose ◽  
Copper Manganese ◽  
High Forage ◽  
Mineral Concentrations ◽  
Cu And Zn ◽  
Mn Concentrations

Abstract Twelve crossbred Angus steers fitted with rumen cannulae were used to determine the influence of trace mineral source on ruminal soluble mineral concentrations and relative binding strength of trace minerals in the ruminal insoluble digesta fraction. Steers were adjusted to a low-quality hay (chopped) diet balanced to meet the nutrient requirements for growing steers. For 7 d prior to initiation of the study, steers were fed the diet without supplemental Cu, Mn, and Zn. On day 8 steers received a pulse dose (2 x NRC requirement) of Cu, Mn, and, Zn from sulfate (STM) or hydroxy trace minerals (HTM; Micronutrients LLC, USA). Ruminal samples were obtained at 2 h intervals until 24 h post-dosing for soluble (fraction obtained after centrifugation) Cu, Mn, and Zn concentrations. Binding strength of Cu, Mn, and Zn to ruminal solid digesta (post centrifugation) was estimated at 0, 12, and 24h post-dosing using dialysis against 0.05 M Tris-EDTA. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 16 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post dosing in STM compared to HTM steers. Concentration of Cu, Mn, and Zn remaining in the insoluble fraction were greater (P < 0.05) at 12 h post dosing in HTM vs STM supplemented steers. At 24 h post dosing, Mn concentrations were lesser (P < 0.03) and Zn concentrations were greater (P < 0.001) in HTM vs STM supplemented steers. Following dialysis, the % Cu, and Zn released from the insoluble fraction was greater (P < 0.001) at 12 h and 24h in HTM vs. STM supplemented steers. Results indicate that a greater proportion of Cu and Zn HTM remained loosely bound to the insoluble fraction within the rumen.

scholarly journals 226 Influence of trace mineral source on digestion, ruminal volatile fatty acid and soluble mineral on steers fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 133-134
Author(s):  
Octavio Guimaraes ◽  
John Wagner ◽  
Jerry Spears ◽  
Terry Engle
Keyword(s):  
Dairy Cow ◽  
Rumen Fluid ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Specific Amino Acid ◽  
Fiber Digestion ◽  
Post Feeding ◽  
Pulse Dose ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 530.9 ± 22.7kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on digestibility, ruminal VFA, and soluble concentrations of Cu, Zn, and Mn. Steers were fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow (DM basis: 16.8% CP, 29.2 Mcal NEm, 30.3 Mcal NEl, 33.6% NDF) for 21 d. Treatments consisted of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxy (HTM; IntelliBond) or organic (ORG; metal specific amino acid complexes) sources (n=4 steers/treatment). Following a 21-d adaptation period, total fecal output was collected for 5 d. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding and analyzed for VFA. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (cannulae) of 100 mg Cu, 400 mg Mn, and 60 mg Zn from either STM, HTM, or ORG. Ruminal samples were obtained at 2-h intervals starting at -4 and ending at 24 h post dosing and analyzed for soluble Cu, Mn, and Zn. Digestibility of NDF and ADF were lesser (P < 0.05) in STM vs. HTM and ORG supplemented steers. Steers receiving HTM and ORG had greater (P < 0.05) total VFA concentrations than STM supplemented steers at 2 and 4h post feeding. Ruminal soluble Cu and Zn concentrations were greater (P < 0.001) post dosing in STM and ORG supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. Results indicate that ruminal solubility of Cu and Zn differs between STM, HTM and ORG sources and trace mineral source impacts fiber digestion and ruminal VFA concentrations.

scholarly journals Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review

2021 ◽  
Vol 22 (7) ◽  
pp. 3800
Author(s):  
Ingmar A. J. van Hengel ◽  
Melissa W. A. M. Tierolf ◽  
Lidy E. Fratila-Apachitei ◽  
Iulian Apachitei ◽  
Amir A. Zadpoor
Keyword(s):  
Systematic Review ◽  
Antibacterial Activity ◽  
Plasma Electrolytic Oxidation ◽  
Bacterial Load ◽  
Titanium Implants ◽  
Future Research ◽  
Resistant Bacteria ◽  
Electrolytic Oxidation ◽  
Plasma Electrolytic ◽  
Cu And Zn

Patients receiving orthopedic implants are at risk of implant-associated infections (IAI). A growing number of antibiotic-resistant bacteria threaten to hamper the treatment of IAI. The focus has, therefore, shifted towards the development of implants with intrinsic antibacterial activity to prevent the occurrence of infection. The use of Ag, Cu, and Zn has gained momentum as these elements display strong antibacterial behavior and target a wide spectrum of bacteria. In order to incorporate these elements into the surface of titanium-based bone implants, plasma electrolytic oxidation (PEO) has been widely investigated as a single-step process that can biofunctionalize these (highly porous) implant surfaces. Here, we present a systematic review of the studies published between 2009 until 2020 on the biomaterial properties, antibacterial behavior, and biocompatibility of titanium implants biofunctionalized by PEO using Ag, Cu, and Zn. We observed that 100% of surfaces bearing Ag (Ag-surfaces), 93% of surfaces bearing Cu (Cu-surfaces), 73% of surfaces bearing Zn (Zn-surfaces), and 100% of surfaces combining Ag, Cu, and Zn resulted in a significant (i.e., >50%) reduction of bacterial load, while 13% of Ag-surfaces, 10% of Cu-surfaces, and none of Zn or combined Ag, Cu, and Zn surfaces reported cytotoxicity against osteoblasts, stem cells, and immune cells. A majority of the studies investigated the antibacterial activity against S. aureus. Important areas for future research include the biofunctionalization of additively manufactured porous implants and surfaces combining Ag, Cu, and Zn. Furthermore, the antibacterial activity of such implants should be determined in assays focused on prevention, rather than the treatment of IAIs. These implants should be tested using appropriate in vivo bone infection models capable of assessing whether titanium implants biofunctionalized by PEO with Ag, Cu, and Zn can contribute to protect patients against IAI.

scholarly journals Independent somatic evolution underlies multifocal small intestine neuroendocrine tumors

2020 ◽  
Author(s):  
Erik Elias ◽  
Arman Ardalan ◽  
Markus Lindberg ◽  
Susanne Reinsbach ◽  
Andreas Muth ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lymph Node ◽  
Regional Lymph Node ◽  
Tumor Development ◽  
Research Direction ◽  
Metastatic Spread ◽  
Future Research ◽  
Intestinal Tumors ◽  
Somatic Evolution ◽  
Clonal Origin

AbstractSmall intestine neuroendocrine tumor (SI-NET), the most common cancer of the small bowel, often displays a curious multifocal phenotype with several intestinal tumors centered around a regional lymph node metastasis, yet the typical path of evolution of these lesions remains unclear. Here, we determined the complete genome sequences of 37 tumors from 5 patients with multifocal SI-NET, allowing elucidation of phylogenetic relationships between multiple intestinal tumors and metastases in individual patients. Notably, lack of shared somatic mutational events strongly supported that the intestinal tumors were of independent clonal origin. Furthermore, adjacent lymph node metastases arose from a single, typically centrally located, intestinal tumor. Thus, we propose that multifocal SI-NETs form primarily by independent somatic evolution rather than local metastatic spread, suggesting a contribution from of a cancer-priming local environmental factor.Significance statementIn multifocal cancer, several tumors arise in the same tissue area. Typically, this is due local metastatic spread, eventually leading to multiple tumors arising from a unique precursor clone, or due to a common heritable oncogenic genetic variant shared among all cells in an individual. Here, we find tumor development is independently initiated in multiple separate cells in a cancer of the small intestine, leading to a development of a polyclonal group of tumors that are genetically unrelated in terms of somatic evolution. This implies a contribution from a yet unknown non-genetic factor, thus providing an important future research direction for an enigmatic form of cancer in which genetic driver events are generally lacking.

scholarly journals Trace mineral source impacts rumen trace mineral metabolism and fiber digestion in steers fed a medium-quality grass hay diet

2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John J Wagner ◽  
Jerry W Spears ◽  
Terry Eugene Engle
Keyword(s):  
Mineral Metabolism ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Time Interaction ◽  
Grass Hay ◽  
Medium Quality ◽  
Mineral Concentrations ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 452.8 ± 6.1 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral (TM) source on digestibility, ruminal volatile fatty acid (VFA) composition, ruminal soluble concentrations of Cu, Zn, and Mn, and relative binding strength of trace minerals located in the rumen insoluble digesta fraction. Steers were fed a medium-quality grass hay diet (DM basis: 10.8% CP, 63.1% neutral detergent fiber [NDF], 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment) to provide 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively. Following a 21-d adaptation period, total fecal output was collected for 5 d. Dry matter (P < 0.07) and CP (P < 0.06) digestibility tended to be reduced, and NDF (P < 0.04) and acid detergent fiber (ADF) (P < 0.05) digestibility were reduced in STM- vs. HTM-supplemented steers. On day 6, ruminal fluid was collected at 0, 2, and 4 h post-feeding and analyzed for VFA. There were no treatment x time interactions for VFA. Steers receiving HTM had less (P < 0.02) molar proportions of butyric acid and greater (P < 0.05) total VFA concentrations than STM-supplemented steers. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On day 15 steers received a pulse dose of 20 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either STM or HTM (n = 6 steers/treatment). Ruminal samples were obtained at 2-h intervals starting at −4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.03) for ruminal soluble Cu, Mn, and Zn concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post-dosing in STM compared with HTM-supplemented steers. Copper concentrations were greater (P < 0.05) at 12 and 24 h and Zn concentrations in ruminal solid digesta were greater at 24 h in HTM-supplemented steers. Upon dialysis against Tris-EDTA, the percent Zn released from digesta was greater (P < 0.05) at 12 h (P < 0.03) and 24 h (P < 0.05), and the percent Cu released was greater (P < 0.02) at 24 h post-dosing in HTM steers when compared with STM-supplemented steers. Results indicate that Cu and Zn from HTM have low solubility in the rumen and appear to be less tightly bound to ruminal solid digesta than Cu and Zn from STM. The lower ruminal soluble concentrations of Cu and Zn in steers given HTM were associated with greater fiber digestibility.

scholarly journals Increasing intake of dietary soluble nutrients affects digesta passage rate in the stomach of growing pigs

2019 ◽  
Vol 121 (5) ◽  
pp. 529-537 ◽  
Author(s):  
Marijke Schop ◽  
Alfons J. M. Jansman ◽  
Sonja de Vries ◽  
Walter J. J. Gerrits
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Feed Intake ◽  
Growing Pigs ◽  
Passage Rate ◽  
Intake Level ◽  
High Feed ◽  
Dietary Nutrient ◽  
Digesta Passage ◽  
Nutrient Solubility

AbstractThe passage rate of solids and liquids through the gastrointestinal tract differs. Increased dietary nutrient solubility causes nutrients to shift from the solid to the liquid digesta fraction and potentially affect digesta passage kinetics. We quantified: (1) the effect of three levels of dietary nutrient solubility (8, 19 and 31 % of soluble protein and sucrose in the diet) at high feed intake level (S) and (2) the effect of lowv.high feed intake level (F), on digesta passage kinetics in forty male growing pigs. The mean retention time (MRT) of solids and liquids in the stomach and small intestine was assessed using TiO2and Cr-EDTA, respectively. In addition, physicochemical properties of digesta were evaluated. Overall, solids were retained longer than liquids in the stomach (2·0 h,P<0·0001) and stomach+small intestine (1·6 h,P<0·001). When S increased, MRT in stomach decreased by 1·3 h for solids (P=0·01) and 0·7 h for liquids (P=0·002) but only at the highest level of S. When F increased using low-soluble nutrients, MRT in stomach increased by 0·8 h for solids (P=0·041) and 0·7 h for liquids (P=0·0001). Dietary treatments did not affect water-binding capacity and viscosity of digesta. In the stomach of growing pigs, dietary nutrient solubility affects digesta MRT in a non-linear manner, while feed intake level increases digesta MRT depending on dietary nutrient solubility. Results can be used to improve predictions on the kinetics of nutrient passage and thereby of nutrient digestion and absorption in the gastrointestinal tract.

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