scholarly journals PSXII-13 The influence of trace mineral source on copper, manganese, and zinc binding strength to rumen digesta in cattle fed a high forage-based diet

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 443-443
Author(s):  
Octavio Guimaraes ◽  
Huey Yi Loh ◽  
Meghan P Thorndyke ◽  
Nicole M Tillquist ◽  
Jerry W Spears ◽  
...  
Keyword(s):  
Insoluble Fraction ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Binding Strength ◽  
Pulse Dose ◽  
Copper Manganese ◽  
High Forage ◽  
Mineral Concentrations ◽  
Cu And Zn ◽  
Mn Concentrations

Abstract Twelve crossbred Angus steers fitted with rumen cannulae were used to determine the influence of trace mineral source on ruminal soluble mineral concentrations and relative binding strength of trace minerals in the ruminal insoluble digesta fraction. Steers were adjusted to a low-quality hay (chopped) diet balanced to meet the nutrient requirements for growing steers. For 7 d prior to initiation of the study, steers were fed the diet without supplemental Cu, Mn, and Zn. On day 8 steers received a pulse dose (2 x NRC requirement) of Cu, Mn, and, Zn from sulfate (STM) or hydroxy trace minerals (HTM; Micronutrients LLC, USA). Ruminal samples were obtained at 2 h intervals until 24 h post-dosing for soluble (fraction obtained after centrifugation) Cu, Mn, and Zn concentrations. Binding strength of Cu, Mn, and Zn to ruminal solid digesta (post centrifugation) was estimated at 0, 12, and 24h post-dosing using dialysis against 0.05 M Tris-EDTA. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 16 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post dosing in STM compared to HTM steers. Concentration of Cu, Mn, and Zn remaining in the insoluble fraction were greater (P < 0.05) at 12 h post dosing in HTM vs STM supplemented steers. At 24 h post dosing, Mn concentrations were lesser (P < 0.03) and Zn concentrations were greater (P < 0.001) in HTM vs STM supplemented steers. Following dialysis, the % Cu, and Zn released from the insoluble fraction was greater (P < 0.001) at 12 h and 24h in HTM vs. STM supplemented steers. Results indicate that a greater proportion of Cu and Zn HTM remained loosely bound to the insoluble fraction within the rumen.

scholarly journals PSXII-14 The influence of trace minerals source on copper, manganese, and zinc binding strength to rumen digesta in cattle fed a corn silage-based diet

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 442-443
Author(s):  
Octavio Guimaraes ◽  
Huey Yi Loh ◽  
Meghan P Thorndyke ◽  
Nicole M Tillquist ◽  
Jerry W Spears ◽  
...  
Keyword(s):  
Bolus Dose ◽  
Corn Silage ◽  
Zinc Binding ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Binding Strength ◽  
Relative Binding ◽  
Copper Manganese ◽  
Cu And Zn ◽  
Mn Concentrations

Abstract Twelve crossbred steers fitted with rumen cannulae were used to determine the influence of trace mineral (TM) source on relative binding strength of minerals in the ruminal insoluble digesta fraction (IDF). Steers (n = 4/treatment) were fed a corn silage-based diet containing no supplemental Cu, Mn, and Zn for 7d. On d 8, steers received a bolus dose (2 x NRC requirement) of the respective TM from sulfate (STM), organic (ORG; provided as Zn methionine, Mn methionine, and Cu lysine), or hydroxy TM (HTM; Micronutrients USA LLC) sources. Ruminal samples were obtained at 2 h intervals starting at -4 and ending at 24 h post dosing. Ruminal samples were centrifuged and supernatant and the IDF were collected and frozen separately. Binding strength of Cu, Mn, and Zn to the IDF was estimated at 0, 12, and 24 h using dialysis against 0.05 M Tris-EDTA. At 0 h pre-dosing, IDF concentrations of Cu, Mn, and Zn were similar across treatments. At 12 h post dosing Cu and Zn IDF concentrations were greater (P < 0.05) in HTM vs. STM and ORG supplemented steers, and Mn concentrations were greater (P < 0.05) in HTM and ORG vs. STM supplemented steers. At 24 h post dosing, IDF Zn concentrations were greater (P < 0.05) in HTM vs STM and ORG supplemented steers and Cu and Mn concentrations were similar across treatments. At 12 and 24 h post dosing the percent Cu and Zn released following dialysis was greater (P < 0.05) in HTM vs STM and ORG supplemented steers. The percent released of Cu, Mn, and Zn at 0 h, and Mn at 12 and 24 h were similar across treatments. Data indicate that a greater concentration of Cu and Zn from HTM is loosely bound to the ruminal IDF.

scholarly journals Trace mineral source impacts rumen trace mineral metabolism and fiber digestion in steers fed a medium-quality grass hay diet

2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John J Wagner ◽  
Jerry W Spears ◽  
Terry Eugene Engle
Keyword(s):  
Mineral Metabolism ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Time Interaction ◽  
Grass Hay ◽  
Medium Quality ◽  
Mineral Concentrations ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 452.8 ± 6.1 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral (TM) source on digestibility, ruminal volatile fatty acid (VFA) composition, ruminal soluble concentrations of Cu, Zn, and Mn, and relative binding strength of trace minerals located in the rumen insoluble digesta fraction. Steers were fed a medium-quality grass hay diet (DM basis: 10.8% CP, 63.1% neutral detergent fiber [NDF], 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment) to provide 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively. Following a 21-d adaptation period, total fecal output was collected for 5 d. Dry matter (P < 0.07) and CP (P < 0.06) digestibility tended to be reduced, and NDF (P < 0.04) and acid detergent fiber (ADF) (P < 0.05) digestibility were reduced in STM- vs. HTM-supplemented steers. On day 6, ruminal fluid was collected at 0, 2, and 4 h post-feeding and analyzed for VFA. There were no treatment x time interactions for VFA. Steers receiving HTM had less (P < 0.02) molar proportions of butyric acid and greater (P < 0.05) total VFA concentrations than STM-supplemented steers. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On day 15 steers received a pulse dose of 20 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either STM or HTM (n = 6 steers/treatment). Ruminal samples were obtained at 2-h intervals starting at −4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.03) for ruminal soluble Cu, Mn, and Zn concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post-dosing in STM compared with HTM-supplemented steers. Copper concentrations were greater (P < 0.05) at 12 and 24 h and Zn concentrations in ruminal solid digesta were greater at 24 h in HTM-supplemented steers. Upon dialysis against Tris-EDTA, the percent Zn released from digesta was greater (P < 0.05) at 12 h (P < 0.03) and 24 h (P < 0.05), and the percent Cu released was greater (P < 0.02) at 24 h post-dosing in HTM steers when compared with STM-supplemented steers. Results indicate that Cu and Zn from HTM have low solubility in the rumen and appear to be less tightly bound to ruminal solid digesta than Cu and Zn from STM. The lower ruminal soluble concentrations of Cu and Zn in steers given HTM were associated with greater fiber digestibility.

scholarly journals 226 Influence of trace mineral source on digestion, ruminal volatile fatty acid and soluble mineral on steers fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 133-134
Author(s):  
Octavio Guimaraes ◽  
John Wagner ◽  
Jerry Spears ◽  
Terry Engle
Keyword(s):  
Dairy Cow ◽  
Rumen Fluid ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Specific Amino Acid ◽  
Fiber Digestion ◽  
Post Feeding ◽  
Pulse Dose ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 530.9 ± 22.7kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on digestibility, ruminal VFA, and soluble concentrations of Cu, Zn, and Mn. Steers were fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow (DM basis: 16.8% CP, 29.2 Mcal NEm, 30.3 Mcal NEl, 33.6% NDF) for 21 d. Treatments consisted of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxy (HTM; IntelliBond) or organic (ORG; metal specific amino acid complexes) sources (n=4 steers/treatment). Following a 21-d adaptation period, total fecal output was collected for 5 d. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding and analyzed for VFA. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (cannulae) of 100 mg Cu, 400 mg Mn, and 60 mg Zn from either STM, HTM, or ORG. Ruminal samples were obtained at 2-h intervals starting at -4 and ending at 24 h post dosing and analyzed for soluble Cu, Mn, and Zn. Digestibility of NDF and ADF were lesser (P < 0.05) in STM vs. HTM and ORG supplemented steers. Steers receiving HTM and ORG had greater (P < 0.05) total VFA concentrations than STM supplemented steers at 2 and 4h post feeding. Ruminal soluble Cu and Zn concentrations were greater (P < 0.001) post dosing in STM and ORG supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. Results indicate that ruminal solubility of Cu and Zn differs between STM, HTM and ORG sources and trace mineral source impacts fiber digestion and ruminal VFA concentrations.

scholarly journals 393 Awardee Talk: Effect of trace mineral source on rumen fermentation and trace mineral distribution in the rumen

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 171-172
Author(s):  
Terry E Engle
Keyword(s):  
Small Intestine ◽  
Insoluble Fraction ◽  
Absorption Efficiency ◽  
Future Research ◽  
Microbial Fermentation ◽  
Trace Mineral ◽  
Passage Rate ◽  
Rumen Microorganisms ◽  
Fiber Digestion ◽  
Cu And Zn

Abstract The role that Cu and Zn play in rumen microbial fermentation is not well understood. Microorganisms use small proportions of dietary Cu and Zn for catalytic, structural, and stabilizing functions. In order for microorganisms to acquire Cu and Zn, the elements must be soluble in the rumen environment. Data would indicate that practical diets fed to ruminants without Cu and Zn supplementation are able to meet the microbial Cu and Zn requirements. Therefore, rumen solubility of supplemental Cu and Zn can impact rumen microbial fermentation characteristics. Numerous factors can impact rumen solubility of minerals, such as the pH of the rumen, the concentration of dietary antagonists (Mo, Fe, S, fiber, etc.), and mineral source. Earlier research has indicated that high soluble concentrations of Cu and Zn in the rumen environment can become toxic to certain rumen microorganisms and decrease fiber digestion. Recent research from our laboratory has indicated that hydroxy trace mineral (HTM) forms of Cu and Zn are relatively insoluble in the rumen and that a greater proportion of HTM remained loosely bound to the insoluble fraction within the rumen when compared to sulfate trace mineral (STM) sources of Cu and Zn. We have also reported that supplemental sources of Cu and Zn that are highly soluble in the rumen environment (STM) can decrease fiber digestion, reduce total VFA concentrations, and can become more tightly bound to rumen solid digesta than HTM sources of Cu and Zn. The stronger binding of STM relative to HTM to the solid rumen digesta fraction may reduce absorption in the small intestine. Future research investigating the flow and passage rate of different trace mineral sources through the abomasum into the duodenum and duodenal absorption efficiency of Cu and Zn from different TM sources is needed.

scholarly journals Comparison of multiple single-use, pulse-dose trace mineral products provided as injectable, oral drench, oral paste, or bolus on circulating and liver trace mineral concentrations of beef steers

2020 ◽  
Vol 36 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Trey D. Jackson ◽  
Remy N. Carmichael ◽  
Erin L. Deters ◽  
Elizabeth M. Messersmith ◽  
Katherine R. VanValin ◽  
...  
Keyword(s):  
Beef Steers ◽  
Trace Mineral ◽  
Pulse Dose ◽  
Single Use ◽  
Mineral Concentrations

scholarly journals Methods of Trace Mineral Supplementation

EDIS ◽  
2018 ◽  
Vol 2018 (5) ◽  
Author(s):  
Matthew J. Hersom ◽  
Todd A. Thrift
Keyword(s):  
Beef Cattle ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Delivery Strategy ◽  
Advantages And Disadvantages ◽  
Mineral Supplementation ◽  
Total Diet ◽  
Animal Growth ◽  
Cattle Producer ◽  
Mineral Concentrations

Trace minerals supplementation is an integral component of the total diet for beef cattle. Trace minerals from the diet function in many of the metabolic processes associated with animal growth, health, and reproduction. It is essential that cattle have access to trace minerals in their diet. However, the forage cattle consume as the bulk of their diet is often deficient in trace mineral concentrations. Therefore, cattle need to be supplemented with trace minerals on a regular basis. Cattle producers can choose from a number of different methods to supplement trace minerals to cattle. Each method has characteristic advantages and disadvantages. The value of the characteristics should be evaluated against management activities of each cattle producer to determine the optimal trace mineral delivery strategy.

Evaluation of iron, copper and zinc concentrations in commercial foods formulated for healthy cats

2021 ◽  
pp. 1098612X2110262
Author(s):  
Stacie Summers ◽  
Jonathan Stockman ◽  
Jennifer A Larsen
Keyword(s):  
Food Products ◽  
Metabolizable Energy ◽  
Life Stages ◽  
Trace Mineral ◽  
Iron Copper ◽  
Copper And Zinc ◽  
Zinc Concentrations ◽  
Dry Food ◽  
Raw Foods ◽  
Mineral Concentrations

Objectives The aim of this study was to analyze iron, copper and zinc concentrations in commercial foods and compare among food formats (dry, canned, raw), foods marketed by age category (adult 1+ years and senior 7+ years) and foods intended for adult maintenance vs all life stages. Methods In total, 112 commercial non-therapeutic food products marketed for healthy adult and senior cats were purchased in the USA. Foods were analyzed for their proximate composition. Trace mineral concentrations were measured using inductively coupled argon plasma–optical emission spectroscopy and described on a calculated metabolizable energy basis using standard modified Atwater values. Results Measured iron (median 58.4 mg/1000 kcal [range 15.7–379.0]), copper (median 5.6 mg/1000 kcal [range 0.8–13.3]) and zinc (median 47.6 mg/1000 kcal [range 7.6–128.1]) concentrations were highly variable among cat foods. When all food products – regardless of their nutritional adequacy substantiation method – were compared with the Association of American Feed Control Officials regulatory minimums, 13/112 food products had a mineral deficiency, of which a majority (n = 11/13) were raw food products. Raw foods had significantly lower trace mineral concentrations compared with dry food products and, except for copper, canned food products. Cat foods marketed for senior cats had higher iron ( P = 0.019) and zinc ( P <0.0001) concentrations than foods marketed for adult cats. Foods intended for adult maintenance had higher iron ( P = 0.003) and zinc concentrations than foods intended for all life stages ( P <0.0001). Conclusions and relevance Iron, copper and zinc concentrations in commercial non-therapeutic foods for adult and senior cats are highly variable. A minority of foods – mainly raw food products – were deficient in these minerals. It is unknown if some foods with high trace mineral concentrations could have adverse effects as studies are needed to establish safe upper limits for dietary intake of trace minerals in healthy cats.

scholarly journals PSXII-17 Trace minerals source influences the performance of Nellore cattle in the growing phase during the transition between dry to rainy season in Brazil

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 445-446
Author(s):  
Iorrano A Cidrini ◽  
Josiane F Lage ◽  
Igor Ferreira ◽  
Karla Oliveira ◽  
Laura F Prados ◽  
...  
Keyword(s):  
Rainy Season ◽  
Relative Bioavailability ◽  
Trace Minerals ◽  
Experimental Unit ◽  
Liver Concentration ◽  
Nellore Cattle ◽  
And Performance ◽  
Urochloa Brizantha ◽  
Cu And Zn ◽  
Matter Basis

Abstract The relative bioavailability of trace minerals may vary between the sources and impact the animal performance. The study was carried out to evaluate the effect of TM source (Cu and Zn) on the liver concentration and performance of Nellore cattle supplemented in the growing phase during the transition between dry to rainy season. A hundred and twenty Nellore young bulls (BW = 349.72 ± 24 kg; 24 months), blocked by BW, were assigned randomly to twelve paddocks (six paddocks/treatment and 10 animals/paddock). Animals grazing Urochloa brizantha cv. Marandu and supplemented at 5 g/kg BW with soybean meal, corn and mineral mix (250 g/kg of CP and 650 g/kg de NDT, natural matter basis). Supplements consisted of 40 mg Cu and 148 mg Zn/kg DM from either inorganic TM (ITM) or hydroxy TM (HTM) Intellibond® sources. Liver samples were collected at the beginning and end of the study (three animals per paddock). Animals were weighed at each period (30 days) during three periods. Period was used as a REPEATED measurement for BW and values of initial BW as independent covariates for all variables. Paddock was considered experimental unit. Data were analyzed by ANOVA using PROC MIXED, SAS 9.4. The animals supplemented with HTM had higher (+0.037 kg/day) general ADG (day 0 to 90; P = 0.012), with 0.469 and 0.506 kg/day to ITM and HTM, respectively. Final BW (day 90) from animals fed HTM was 5.13 kg higher than ITM (391.97 vs. 397.11 kg BW; P = 0.030). No differences were found in the liver concentrations of Cu and Zn between ITM or HTM (P ≥ 0.288). Cu and Zn from HTM improve the performance of animals supplemented in pasture compared to ITM sources.

scholarly journals 99 Effects of feeding 60% dried corn distillers grains plus solubles or the equivalent sulfur as calcium sulfate on trace mineral concentrations in serum and seminal plasma

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 80-81
Author(s):  
Cierrah J Kassetas ◽  
Joel Caton ◽  
James D Kirsch ◽  
Sheri T Dorsam ◽  
Kacie L McCarthy ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Plasma Treatment ◽  
Seminal Plasma ◽  
Repeated Measures ◽  
Calcium Sulfate ◽  
Distillers Grains ◽  
Trace Mineral ◽  
Corn Distillers ◽  
Mineral Concentrations ◽  
Half Sibling

Abstract Thirty-six half-sibling yearling Angus bulls [256 ± 8 d; initial BW = 320 ± 2 kg] were assigned one of three diets: 1) 60% concentrate as corn (CON; n = 12); 2) 60% DDGS (60DDGS; n = 12); 3) CON diet + equivalent sulfur of 60DDGS as CaSO4 (SULF; n = 12) to evaluate effects of feeding 60% DDGS or sulfur as CaSO4 on trace mineral (TM) concentrations in serum and seminal plasma. Bulls were fed in Calan gates for 112 days and targeted to gain 1.6 kg/d. Data were analyzed as repeated measures using PROC MIXED in SAS. In serum, treatment × day interactions were observed (P ≤ 0.03) for Cu, Se, and Mo. For Cu, no differences (P &gt; 0.15) were observed at d 0 or 56, but at d 112, 60DDGS was reduced (P &lt; 0.01) compared with SULF and CON. At d 0, no differences (P &gt; 0.09) were observed for Se; however, at d 56 and 112, 60DDGS was greater (P &lt; 0.01) than CON and SULF. For Mo, at d 0, 60DDGS was greater (P = 0.03) than CON, whereas SULF was intermediate, but at d 56 and 112, CON was greater (P &lt; 0.01) than SULF and 60DDGS. In seminal plasma, treatment × day interactions were observed (P ≤ 0.02) for Cu and Mo. For Cu, no differences (P ≥ 0.09) were observed at d 0 or 56, but at d 112, CON and 60DDGS were greater (P &lt; 0.01) compared with SULF. For Mo, at d 0, 60DDGS was greater (P = 0.03) than SULF, whereas CON was intermediate, but on d 56 and 112, CON was greater (P &lt; 0.01) than 60DDGS and SULF. Differences observed for TM may have influenced enzyme activity and semen kinematics which were previously reported.

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